glycogen synthase kinase 3 β

... Diabetes 43, 9 03 907 21 Orena, S.J., Torchia, A.J & Garofalo, R.S (2000) Inhibition of glycogen- synthase kinase stimulates glycogen synthase and glucose transport by distinct mechanisms in 3T3-L1 ... PKBSer4 73, p70S6K, p42/p44 MAP kinases or GSK3 in L6 myotubes or 3T3-L1 adipocytes (Fig 2) It is noteworthy that in L6 myotubes insulin induces phosphorylation of both GSK3 a and b, whereas in 3T3-L1 ... protein) from L6 myoblasts, L6 myotubes, 3T3-L1 fibroblasts and 3T3-L1 adipocytes were immunoblotted using antibodies against GSK3a and b Ó FEBS 20 03 3 832 K MacAulay et al (Eur J Biochem 270)...

Ngày tải lên: 08/03/2014, 08:20

... Rhythms 2007, 5 :3 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 Hoeflich KP, Luo J, Rubie EA, Tsao MS, Jin O, Woodgett JR: Requirement for glycogen synthase kinase- 3beta in cell ... Chronobiol Int 19 93, 10(5) :32 1 -33 0 Grimes CA, Jope RS: CREB DNA binding activity is inhibited by glycogen synthase kinase- 3 beta and facilitated by lithium J Neurochem 2001, 78(6):1219-1 232 Yin L, Wang ... clock Neuron 2004, 43( 4):527- 537 Iitaka C, Miyazaki K, Akaike T, Ishida N: A role for glycogen synthase kinase- 3beta in the mammalian circadian clock J Biol Chem 2005, 280 (33 ):2 939 7-402 Takahashi...

Ngày tải lên: 10/08/2014, 09:20

Ngày tải lên: 14/11/2014, 09:42

... complementarity determinant 133 (CD 133 )31 , stage-specific embryonic antigen (SSEA-1 )32 , Nestin 33, aldehyde dehydrogenase (ALDH )33 and the Side Population (SP )34 , 35 Many of these markers are ... xiii Figure -33 Transduction of NNI-4 and NNI-8 GPCs with GSK 3β shRNA 106 results in an ablation of total GSK 3β and activating phosphorylated Tyr216 (GSK 3β) Figure -34 Ablation of GSK 3β reduces cell ... expression levels 111 Figure -37 GSK 3β downregulation by shGSK 3β reduces stemness expression and induces differentiation in NNI-4 1 13 Figure -38 GSK 3β downregulation by shGSK 3β reduces stemness expression...

Ngày tải lên: 03/10/2015, 21:57

... 0.8 pGSK 3β β 0.4 GSK 3β 0 60 120 180 240 30 0 LPS Time (min) 50 100 500 LPS (ng·mL–1) C 10 1.8 1 .3 0.7 0.5 0.9 0.7 0.6 (fold) C Nox1 pGSK 3β β -actin 0.5 C3H/HeN (TLR4 WT) 0.5 LPS (h) C3H/HeJ (TLR4 ... GSK3b-b-catenin is involved in Nox1 expression A β- catenin β- actin 15 30 45 60 LPS (min) B β- catenin β- actin 10 50 100 500 LPS (ng·mL–1) C β- catenin (short) β- catenin (long) β- tubulin 15 30 45 ... ª 2010 FEBS 2 831 GSK3b-b-catenin is involved in Nox1 expression J.-S Kim et al A A 1.6 1.9 1.4 0.7 (fold) Nox1 pGSK 3β ROS production (RLU) β- actin 1.2 GSK 3β LPS (h) B Con LPS 15 30 45 60 LPS (min)...

Ngày tải lên: 22/03/2014, 21:21

... FEBS 14 -3- 3 Controls cardiomyocyte hypertrophy through GSK3b NE α1-AR Hypertrophic stimuli Cytoplasm PI3K PKC 14 -3- 3 PKB Inactive Active NFAT P P 14 GSK 3β GSK3 -ser-9-P GSK 3β GSK3 NFAT 14 -3- 3 W Liao ... 18sRNA 14 -3- 3 protein B 12 24 48 time (h) 14 -3- 3β 14 -3- 3 ζ 14 -3- 3 ε 14 -3- 3 γ Fig (A) Different isoforms of 14 -3- 3 s were expressed in cardiomyocytes 14 -3- 3f, e, c and b isoforms expressed in isolated ... hypertrophy ERK1/2 Phospho-PKB PKB Phospho-GSK 3β GSK 3β NE AdR18 LY Phospho-PKB PKB Phospho-GSK 3β GSK 3β GSK 3β phosphorylation fold of control GSK 3β phosphorylation fold of control B * * CON NE...

Ngày tải lên: 16/03/2014, 18:20

... GFP–HsMGS 1a 1b 22a 3abc 45 22a3abc45 22a3abc451ab R1 R2 R1 ⁄ R2 20.7 47 .3 21.2 31 .3 34.4 39 .4 36 .5 30 .7 22.8 60.0 32 .4 51 .3 104.4 54.4 67.7 60 .3 96.0 74.6 53. 9 34 .8 52.9 36 .1 0.40 0.45 0 .39 0.46 0.57 ... Fletterick RJ (1989) Human muscle glycogen synthase cDNA sequence: a negatively charged protein with an asym- FEBS Journal 272 (2005) 31 97 32 13 ª 2005 FEBS E Cid et al 35 36 37 38 metric charge distribution ... FEBS Journal 272 (2005) 31 97 32 13 ª 2005 FEBS 32 09 Nucleocytoplasmic translocation of muscle glycogen synthase Cell culture and transfection 2 93 Fibroblasts (ATCC No CRL-15 73, Manassas, VA, USA)...

Ngày tải lên: 16/03/2014, 22:20

... phosphorylation of GST–TSSK3 with [32 P]ATP[cP] The phosphorylation reactions of GST–TSSK3K39R by Myc-PDK1 [32 ] immunoprecipitated from 293T cells and of HA–TSSK3K39R immunoprecipitated from 293T cells, by ... of TSSK3 M Bucko-Justyna et al Myc-PDK1 immunoprecipitated from 293T cells efficiently phosphorylated GST–TSSK3K39R (Fig 3E, upper) and haemagglutinin epitope tagged (HA)– TSSK3K39R (Fig 3E, middle) ... signal-transduction pathway J Biol Chem 279, 33 759 33 767 28 Burgering BM & Coffer PJ (1995) Protein kinase B (c-Akt) in phosphatidylinositol -3- OH kinase signal transduction Nature 37 6, 599–602 29 Dong LQ, Ramos...

Ngày tải lên: 23/03/2014, 11:20

... cyclin for p35cdk3 For this purpose, we cotransfected COS7 cells with expression vectors for ik3-1 and cdk3 A kinase assay with p35cdk3 immunoprecipitated from COS7 cells indicated that p70ik3-1 did ... tyrosine phosphorylation, kinase upregulation, and neurite outgrowth Neuron 26, 633 – 646 Lew, J & Wang, J.H (1995) Neuronal cdc2-like kinase Trends Biochem Sci 20, 33 37 Matsuoka, M., Tani, K ... of ik3-1 increased only in lysates from cells where cyclin A and wild-type cdk3, not cdk3 dn, were expressed, supporting the theory that cyclin/ cdk3 actually phosphorylates ik3-1 P70ik3-1 is...

Ngày tải lên: 24/03/2014, 04:21

... control animals were unremarkable and had a mean damage score of 3. 7 ± 0 .3 (Figure 3) In contrast, in both no pump (12.7 ± 1.4) and PEG 30 0 vehicle alone (10.7 ± 1.4) treatment groups, portions of ... membrane and induction of TNFα production [32 ,33 ] A soluble fragment of the murine IL-15Rα chain inhibited development of collagen-induced arthritis in DBA/1 mice [34 ] Administration of an IL-15 mutant/Fcγ2c ... rejection by a specific janus kinase inhibitor Science 20 03, 30 2:875-878 Kudlacz E, Perry B, Sawyer P, Conklyn M, McCurdy S, Brissette W, Flanagan M, Changelian P: The novel JAK -3 inhibitor CP690550 is...

Ngày tải lên: 09/08/2014, 10:22

... – 12 .3 12 .3 – 48 48.7 – 12.2 12.2 – 48.7 33 .8 – 33 33 .8 – 33 2.4 – 17.8 17.8 – 2.4 16.2 – 2.5 2.5 – 16.2 6.4 – 6.4 6.4 – 6.4 17.7 – 2.4 2.4 – 17.7 13 – 3. 6 3. 6 – 13. 9 3. 6 – 13. 9 13. 9 – 3. 6 7–7 ... 7–7 1 .3 – 1 .3 1 .3 – 1 .34 1 .34 – 1 .34 1 .34 – 1 .34 1 .34 – 1 .34 1 .34 – 1 .34 1 .34 – 1 .34 1 .34 – 1 .34 0.5 – 50 0.07 – 70 0.04 – 0.11 – 11 – 100 0.5 – 50 1.5 – 150 0.012 – 1.2 49 – 13. 6 40.5 – 27 32 – ... 33 .8 – 33 .8 33 .8 – 33 .8 11.6 – 2.7 3. 9 – 19.4 6–9 16 .3 – 2.5 6.4 – 6.4 6.4 – 6.4 3. 8 – 16 – 7.8 5.9 – 12.9 18 – 2.6 11.9 – 3. 1 11.4 – 8.8 7–7 7–7 7–7 7–7 1.85 – 1.2 1.85 – 1.1 1 .34 – 1 .34 1 .34 ...

Ngày tải lên: 13/08/2014, 22:22

... protein GK Glucokinase GKRP Glucokinase regulatory protein GPh Glycogen phosphorylase GS Glycogen synthase GSD Glycogen storage disorder GSK3 Glycogen synthase kinase Gsy2p Glycogen synthase isoform ... receptor,βAR-βAdrenergic Receptor, GR- Glucagon receptor, PK – Protein Kinase, PI3K – Phosphotidyl inositol kinase, GSK3- Glycogen Synthase Kinase, PP1G- Protein Phosphatase, Gs- Glycogen Synthase, ... REGULATION OF YEAST GLYCOGEN SYNTHASE Glycogen is a major energy reserve in most eukaryotes and its rate of synthesis is controlled by glycogen synthase The activity of eukaryotic glycogen synthase is...

Ngày tải lên: 24/08/2014, 12:49

... GAPDH–10aa–citrine 37 .1 42.2 43. 4 43. 8 31 .2 41.1 1 .39 1.19 1 .32 1 .33 1 .37 1.24 ± ± ± ± ± ± a2 (%) 0.05a 0.07a 0.05c 0.05c 0.06b 0.12b 62.9 58.8 56.6 56.2 68.8 58.9 3. 62 3. 27 3. 46 3. 39 3. 56 3. 31 n s2 (ns) ... Cerulean–GAPDH Citrine–GAPDH PGK 36 .8 39 .6 39 .9 41.4 1.41 1.06 1. 43 1.29 ± ± ± ± a2 (%) 0.21a 0.09a,b 0.20 0.05b 63. 2 60.4 60.1 58.6 3. 59 3. 13 3. 63 3.44 n s2 (ns) ± ± ± ± 0.13a 0.07a,b 0.19 0.10b v2 ... Fraction no 25 30 35 WB : anti-GFP 25 35 45 WB : anti-GAPDH Fraction no PGK activity / mU C 138 50 69 44 kDa Fraction no 40 45 55 WB : anti-GFP 20 10 40 50 60 Fraction no 131 2 50 30 WB : anti-PGK...

Ngày tải lên: 16/02/2014, 09:20

... in single, human platelets Regulation by 38 4 30 31 32 33 34 35 36 37 38 39 40 41 modulation of the inositol trisphosphate receptors Eur J Biochem 269, 15 43 1552 Fischer L, Gukovskaya AS, Young ... thrombin; *P < 0.05 (n = 4–6) 37 4 ± ± ± ± ± ± 38 (100%) 69 (91%)* 142 (100%) 131 (119%) 1 03 ( 93% )* 131 (87%)* ± ± ± ± ± ± 30 32 2660 4098 7418 2784 34 21 (100%) (66%)* (72%)** ( 135 %)* (64%)* (59%)* This ... hand, pretreatment with the PI3-Kc-specific inhibitor, AS252424 [21], was without effect As protein kinase B ⁄ Akt is a downstream mediator of PI3-K in platelets [33 ,34 ], the effects of the isoform-specific...

Ngày tải lên: 18/02/2014, 16:20

... neuraminidase Structure 6, 735 –746 13 Ely B & Pittard J (1979) Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12 J Bacteriol 138 , 933 –9 43 14 Hennig M, Darimont ... compilation ª 2008 FEBS 1 53 Inhibitor of indole -3- glycerol phosphate synthase H Shen et al erol-phosphate synthase from Escherichia coli Methods Enzymol 142, 38 6 39 7 37 Anderotti G, Cubellis MV, ... Mycobacterium tuberculosis indole -3- glycerol phosphate synthase Biochemistry (Moscow) 71, S38– S 43 Amzel L (1998) Structure-based drug design Curr Opin Biotechnol 9, 36 6 36 9 10 Greer J, Erickson J,...

Ngày tải lên: 07/03/2014, 03:20

... BL21(DE3) Escherichia coli strain pET3a pET3a pET3a BL21-CodonPlus (DE3) BL21(DE3)plysS BL21(DE3)plysS pET3a pET3a BL21-CodonPlus (DE3) BL21(DE3)plysS pET15b pET3a pET15b Vector LB LB LB M9 (AA)b ... 274 (2007) 436 0– 437 4 ª 2007 The Authors Journal compilation ª 2007 FEBS 436 3 Bacterial fructosamine 3 -kinase homologues R Gemayel et al Fig Alignment of human fructosamine 3 -kinase (FN3K) and fructosamine ... Journal 274 (2007) 436 0– 437 4 ª 2007 The Authors Journal compilation ª 2007 FEBS 436 1 Bacterial fructosamine 3 -kinase homologues R Gemayel et al Ribulosamine Associated Associated 3 -kinase LMW-PTP...

Ngày tải lên: 07/03/2014, 05:20

... Downward J & Evan G (1997) FEBS Journal 2 73 (2006) 234 5– 235 6 ª 2006 The Authors Journal compilation ª 2006 FEBS E.M Harrison et al 28 29 30 31 32 33 34 35 36 37 38 39 Suppression of c-Myc-induced apoptosis ... 30 (A) Other groups were pretreated with the PI3K inhibitor LY294002 (B), or its inactive analog LY3 035 11 (C) for 30 min, and then treated with insulin (200 nM) for 30 to determine glycogen synthase ... HO-1 is PI3K dependent In our model, phosphorylation of glycogen synthase kinase (GSK3b) was used as an indicator of PI3K ⁄ Akt axis activity GSK3b phosphorylation was observed after 30 of insulin...

Ngày tải lên: 07/03/2014, 12:20

... the brain kinase that phosphorylated 14 -3- 3f on Thr 233 [33 ] 14 -3- 3s and yeast 14 -3- 3s (BMH1 and 2) were also phosphorylated on the equivalent sites In vivo phosphorylation of 14 -3- 3f at this ... antibodies that recognize all 14 -3- 3 isoforms (PAN) FEBS Journal 272 (2005) 37 67 37 76 ª 2005 FEBS B 37 69 BCR kinase phosphorylates 14 -3- 3s on residue 233 S J Clokie et al 14 -3- 3b, c, e, f, s and g isoforms ... SJ & Cantley LC FEBS Journal 272 (2005) 37 67 37 76 ª 2005 FEBS S J Clokie et al 23 24 25 26 27 28 29 30 31 32 33 34 35 (1997) The structural basis for 14 -3- 3: phosphopeptide binding specificity Cell...

Ngày tải lên: 07/03/2014, 21:20

... major PKC substrate, 80K/MARCKS, 36 4 G Wein et al (Eur J Biochem 270) 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 increases sharply when Swiss 3T3 cells move out of cycle and enter ... MARCKS 3 -UTR were driven by a CMV-promoter and used for transfection of Swiss 3T3 fibroblasts Length of the MARCKS 3 -UTR sequences [38 ] of pDK1: 131 0–2597 bp (complete 3 -UTR of MARCKS); pDK2: 131 0– ... RNA element in Swiss 3T3 cells 36 0 G Wein et al (Eur J Biochem 270) Ó FEBS 20 03 Fig HuR is expressed in the nucleus and binds the 52 nt CU-rich RNA (A) Quiescent Swiss 3T3 cells were harvested...

Ngày tải lên: 08/03/2014, 08:20