gfp rnai over expressing pcrl uida constructs and transgenic plants

... was the leader of the project SY and WZ amplified the complete genome and analyzed the genome profile QS and FH went on the sequence alignment All authors read and approved the final manuscript ... SaKaeo-15/Thailand Sapovirus Mc2/Japan Sapovirus Ehime1107/2002/JP Sapovirus Mc114/Japan Sapovirus Hu/Dresden/pJG-Sap01/DE Sapovirus NongKhai-24/Thailand Porcine enteric sapovirus/USA Norovirus mouse/Hannover1/2007/DEU ... genomic and predicted subgenomic RNAs The Kozak context, favorable for translation initiation, is underlined C Aligned nucleotide and predicted amino acid sequences at the junction between ORF1 and...

Ngày tải lên: 12/08/2014, 04:21

... procedure, and the modified process is highly specific, low-cost and highly efficient [32] However, the high-throughput GLGI procedure still involves many steps, and is thus very time-consuming Moreover, ... shake, and place at °C; after 2–3 h shake again; after another 2–3 h, aspirate aqueous layer; aliquot and store at )20 °C] and high-concentration ethanol precipitate (see SAGE protocol), and resuspend ... product isolation and sequencing After visualizing specific PCR product on an agarose gel, excise and purify individual bands Sequencing of purified PCR product can be performed by the standard dideoxynucleotide...

Ngày tải lên: 07/03/2014, 00:20

... contributions DYC and CCK designed and performed all the experiments and drafted this manuscript together DYC participated in the sequence alignment and statistical analysis JHH and YCW helped with ... hypotheses need not to be nested, and it does not rely on standard likelihood assumptions In other words, the starting trees in Bayesian method are randomly chosen, and multiple runs of the same ... the Thailand isolates into genotype II Genotype III of DENV-3 consists of the strains from Sri Lanka, India, Africa and Samoa that were recently introduced into Central and South America and caused...

Ngày tải lên: 20/06/2014, 01:20

... 5¢-GATATCATGTCAATGTCCTATACGTGGAC-3¢ and NS5Bj4r 5¢-AAACTCGAGGCGGGGTCGGGCACGAGA CAGG-3¢ The PCR fragment was cleaved with restriction enzymes EcoRV and Xho1 and inserted between the Nde1 site (blunted by Klenow enzyme) and the ... GCATGAGCTACCACCTGGACC-3¢ and VB3: 5¢-CTCG AGCTTCACAAGAAACTTCTGC-3¢ The PCR fragment was cleaved by the restriction enzymes Nde1 and Xho1 and inserted between the Nde1 and the Xho1 sites of the ... 3¢-end of the HCV minus-strand RNA in vitro and that deletion of the 3¢ 45 nt greatly impaired RNA synthesis [17] Oh et al [18] showed that RNA synthesis from the HCV minus-strand RNA required a minimum...

Ngày tải lên: 20/02/2014, 01:20

... recognition of long noun sequences, and in Section we present our proposed solution and describe its implementation Sections and are devoted to related work and to our conclusions, respectively ... given string of nouns See (Finin, 19~0) and (Reimold, 1976) for extensive work in this area, and also (Brachman, 1978) and (Borgida, 1975) Such work by and large assumes that the noun sequence ... sentence and determining its boundaries For the purpose of describing design specifications and requirements a declarative representation language was devised, called SRL (Specification and Requirements...

Ngày tải lên: 08/03/2014, 18:20

... with and without mm EGTA, respectively, gave identical results and therefore suggest that this interaction is calcium independent Interaction between cardosin A and PLDa is mediated through RGD and ... [55] and de novo sequencing were used to identify the protein Extract preparation and immunoprecipitation Mature pistils (200 mg) were ground in a mortar and pestle under liquid nitrogen, and ... resuspended in 50 mm Tris ⁄ 50 mm NaCl (pH 7.4) and lysed with lysozyme (100 lgÆmL)1) After freezing and thawing, DNase (100 lgÆmL)1) and MgCl2 (100 mm) were added, and the reaction mixture was incubated...

Ngày tải lên: 30/03/2014, 11:20

Ngày tải lên: 12/06/2014, 16:07

Ngày tải lên: 12/06/2014, 16:13

... 2.1 when the random |x|p variables Xn are replaced by Xn /an and φn x Theorem 2.3 Let {Xn , n ≥ 1} be a sequence of arbitrary random variables Let Fn and F0 {Ω, Φ}, n ≥ Let φn and hn be defined ... the main theorems of this paper extend the main results by Wang and Guo in 2009, Qiu and Yang in 1999, and the result by Wang and Yang in 2005, respectively The remainder of this paper is organized ... Inequalities and Applications sequences in 2003 In 2008, W Yang and X Yang proved two strong limit theorems for stochastic sequences, which generalized results by Freedman , Isaac, 10 and Petrov Qiu and...

Ngày tải lên: 21/06/2014, 07:20

... signal and therefore Z(n) and W(n) are statistically independent of ea (n) and moreover E{ea (n)eT (n)} = E{ea (n)} · S where S ≈ IP×P for small μ a T ) for large μ where 1T = [1, 0, , 0] and ... steady-state MSE and the simulated steady-state MSE is observed In Figures 16 and 17, we presented the results for N = 3, and Simulation results show good agreement for both colored and uniform input ... input signals Figures 18 and 19 show the steady-state MSE of SPUAPA as a function of step size for M = 16, and different input signals The parameters K, and P were set to 4, and the step size changes...

Ngày tải lên: 21/06/2014, 08:20

... (n) búp bê Cheek: R O R and rewrite the vocabs * Pre- questions - Ask students to look at the pictures - Look at the Guessing on page 16 and answer the following pictures and questions answer the ... 1 Pre - listening *New words: - Listen and guess - announcement: the the meanings New words: (translation): Thông báo of the words from ... Listening: While - listening - Listen to the *Answer the question - Ask Ss to listen to the tape and tape to check the How old is she? check the answers answers Where was she last Feed back - evaluation...

Ngày tải lên: 03/07/2014, 19:20

... objects,chararct ers - Listen and show on the characters - Listen and choral repetition Asking groups a crayon, crayons; a pencil case, pencil cases; a table, tables; ask and a cassette, cassettes; ... Jenny: Scott: 22: pageIt’s a pencil case Andy: What are these?  They’re cassettes Kate: Devidin g class into 02 groups Showin g teacher’ s cards answer - Listen and choral repetition - Group A: What ... notebooks.answer Vocabulary Practice:  What’s this? It’s a crayon - Listen and show on the objects, page 23 - Listen and choral Hanging repetition the wall chart What are these? They’re caryons...

Ngày tải lên: 23/07/2014, 15:20

... uncertain unclear ungrateful unwilling 6 .and you have a strong desire to be of them and go to sleep riddance ridden rid ride 7.you could be subtle and up the sitting room clock wind stir turn ... middle of the conversation restrictions inhibitions prohibitions cprohibitions 10 .And if all else go upstairs and come down again in your pyjamas falls fell fails feels ... sack, which need be no of the softness of the mattress criterion critique criticism critical 3 .And very important people are not described as simply going to bed but rather retiring for the...

Ngày tải lên: 25/07/2014, 07:20

... uncertain unclear ungrateful unwilling 6 .and you have a strong desire to be of them and go to sleep riddance ridden rid ride 7.you could be subtle and up the sitting room clock wind stir turn ... middle of the conversation restrictions inhibitions prohibitions cprohibitions 10 .And if all else go upstairs and come down again in your pyjamas falls fell fails feels ... sack, which need be no of the softness of the mattress criterion critique criticism critical 3 .And very important people are not described as simply going to bed but rather retiring for the...

Ngày tải lên: 25/07/2014, 11:21

... AR and EMa carried out the molecular genetic studies AF and JMaydt performed sequence analysis and wrote and adopted software PM participated in the computer analysis WS, TL, AM, CLM, EMe, and ... sequences from HML-2 proviruses on chromosomes and 11 Recombination events were predicted to have occured between nt 415 and 422, and between nt 583 and 651, resulting in an explanation with zero ... binding regions, and therefore are, for HERV-KX sequences B262, B12 and 93, below the

Ngày tải lên: 13/08/2014, 05:22

... of over- prediction, genesplitting and incorporation of random and transposon-based ORFs into gene models Masking of repeat sequences before gene prediction reduced the number of gene models and ... to develop and improve it deposited research Manual annotation and RT-PCR Gene models (see Table 2) were predicted only if they had supporting EST and comparative evidence and did not overlap with ... DNA and/ or gaps or were present at the end of a supercontig http://genomebiology.com/2007/8/5/R88 Orthology and synteny When compared with the Anopheles and Drosophila gene sets (Table 3), 50 and...

Ngày tải lên: 14/08/2014, 07:21

... Australia and China a Whalley Island and a Condor Reef? On that dangerous coral formation the celebrated clipper had stranded for three days, her captain and crew throwing her cargo overboard ... on the beach And so on, in and out, picking up coastwise cargo here and there, and finishing with a hundred miles' steady steaming through the maze of an archipelago of small islands up to a ... the tide, or seven against Then you steered straight for the land, and by -and- by three palms would appear on the sky, tall and slim, and with their disheveled heads in a bunch, as if in confidential...

Ngày tải lên: 06/11/2012, 17:32

... ………………………………………………………… …………………………………… Tom is trying to learn English He wants to travel to England ………………………………………………………… …………………………………… My class was happy We won the football match last Saturday ... conservationist arrived ( cleaned/ cleaning/ are cleaning/ were cleaning) …….are used to kill inserts and weeds so as to help crops grow better.( Pesticides / Dynamite/ Treasures / Creatures) III/ Put...

Ngày tải lên: 20/07/2013, 01:28

... performance of candidates from occasion to occasion, and the reliability of the scoring Therefore, he suggests several ways of achieving consistent performances from candidates and scorer reliability: ... not allow candidates too much freedom - Write unambiguous items - Provide clear and explicit instructions - Ensure that tests are well laid out and perfectly legible - Make sure candidates are ... of candidates Meanwhile, in a subjective test, candidates must think of what to say and then express their ideas as well as possible Since subjective tests allow for much greater freedom and...

Ngày tải lên: 07/09/2013, 13:19

Ngày tải lên: 25/10/2013, 19:20