genomic dna end sequencing bac fosmid and paired end

... and c¢¢), spermidine (lanes d, d¢ and d¢¢) or putrescine (lanes e, e¢ and e¢¢) and then exposed to DNase I Whole genomic DNA (lane a) and DNase I-digested genomic DNA (lane b) were controls (B) ... whole genomic DNA (lane a) and the DNA fully digested by DNase I (lane b) The DNA kb ladder marker was in lane m Fig Electrophoresis of genomic DNA preincubated with single polyamines and then ... of genomic DNA preincubated with 600 lM spermine (lane c), spermidine (lane d) or putrescine (lane e) and exposed to DNase I Controls were in lanes a (whole genomic DNA) and b (DNA exposed to DNase...

Ngày tải lên: 23/03/2014, 15:20

... section in Of Pandas and People, the ID textbook supplement for junior high and high school biology courses that tries to make the case that ID is legitimate science (Hartwig and Meyer [1989] ... reason, the existence of a real and uniformly ordered universe, and the ability present in a creative and ordered human intellect to know that universe Both the Old and New Testaments define these ... “nothing less than the overthrow of materialism and its cultural legacies” and the renewal of “a broadly theistic understanding of nature.”10 Thaxton and Meyer say that according to the modern view,...

Ngày tải lên: 01/11/2013, 07:20

... expression by DNA methylation Cytosine methylation at the DNA sequence d(GGCGCC)2 triggers an extended eccentric double-helix structure called E -DNA Like B -DNA, E -DNA has a long helical rise and the ... DNA methylation-mediated nucleosome dynamics S K Patra and M Szyf stretches of DNA repeats, replicates in late S phase and contains lower levels of acetylated histones and higher levels of DNA ... chaperones (ASF1A and NPM2), DNMT1 and several Polycomb group proteins (BMI1, EED and EZH2) Several recent studies have linked Polycomb proteins to abnormal DNA methylation and DNA methylation-mediated...

Ngày tải lên: 18/02/2014, 14:20

... including DNA repair kinase DNA- PK, is mobilized onto DNA with ends in a cell-free system, and that it produces the characteristic ‘coacervate-like organization’, which greatly stimulates DNA end- joining ... (lanes and 8) ssDNA induced a noticeable accumulation of 95 k and nucleolinD proteins in addition to DNA- PKcs, Ku80 and Ku70 (lane 9) On the other hand, a relaxed form of closed circular doublestrand ... those that aggregate with nicked DNA, and that some of them also assemble with ssDNA Interestingly, the components of DNA- dependent protein kinase, DNA- PKcs, Ku80 and Ku70, were the common factors...

Ngày tải lên: 19/02/2014, 06:20

Ngày tải lên: 05/03/2014, 16:20

... complete cDNA sequence coding for zebrafish FABP4 was determined by aligning and combining all 3¢- and 5¢-cDNA end sequences Phylogenetic analysis Phylogenetic analysis of zebrafish fabp4 and fabp3 and ... threedimensional structure and ligand-binding specificity and affinity of FABP3 and FABP4, the transcripts and proteins of the two paralogous genes exist in multiple tissues and are colocalized in ... analysis resolved fish FABP4 (and H6-FABPs) and FABP3 (and H8-FABPs) as proteins encoded by distinct genes, and suggested that these genes are orthologs of mammalian FABP4 and FABP3, respectively To...

Ngày tải lên: 07/03/2014, 10:20

... synthesized strand between the nick and the mismatch is carried out by four redundant single-strand DNAspecific exonucleases: the 3¢ fi 5¢ exonucleases ExoI and ExoX and the 5¢ fi 3¢ exonucleases RecJ and ... ExoVII [22] DNA polymerase III, single-stranded DNA- binding protein and DNA ligase carry out repair synthesis [3] Eukaryotic mismatch repair All eukaryotic organisms, including yeast, mouse and human, ... single-stranded DNA to produce UG mismatches (B) MutSa and MutLa are recruited to the mismatched DNA, and activate ExoI (C) The gaps generated by the activity of ExoI are refilled by error-prone DNA...

Ngày tải lên: 07/03/2014, 12:20

... analyse DNA induced conformational changes on hRad51 protein hRad51 (25 lM) was incubated in binding buffer in the absence (lanes and 3) or presence of 75 lM ssDNA (lanes and 5) and mM ATP (lanes and ... states of hRad51 and hRad52 in the presence and absence of ssDNA Our studies indicate that human Rad51, like its bacterial homologue RecA exists in multiple aggregation states [14,32] DNA seems to ... Disassembly and recruitment of hRad51 and hRad52 proteins shorter and thicker nucleoprotein filaments when ssDNA is added [28] Tumour suppressors BRCA2 and p53 interact with the...

Ngày tải lên: 16/03/2014, 14:20

Ngày tải lên: 23/03/2014, 03:20

... protein DnaA and this interaction is essential for DNA replication in Helicobacter [14,15] One protein that is central to the DNA replication, repair and recombination is single-stranded DNA binding ... majority of the active bacillary forms show clear distinct foci of HpDnaB and HpSSB (wherever B C Fig Immunolocalization of HpDnaB and SSB proteins in H pylori bacillary and coccoid forms (A) ... regarding bacterial physiology and growth These results further enhance our knowledge on SSB proteins from a slow growing pathogenic bacteria and offer great potential to study DNA protein and protein–protein...

Ngày tải lên: 30/03/2014, 02:20

... induced substitutions Bacterial strains and plasmids The Escherichia coli strains DH5a [32] and JM105 [33] were used for DNA manipulation and sequencing, and BL21 was used [34] for recombinant ... metallothionein L Villarreal et al Fig Structure and synteny of the chicken, mouse and human genomic regions containing the MT genes The chromosome number and bands enclosing the MT cluster are indicated ... lm, 300 lm and 500 lm, respectively Cloning of the chicken MT cDNA and its separate a and b domains for recombinant expression The ckMT coding sequence, kindly provided by Dr G.K Andrews of the...

Ngày tải lên: 30/03/2014, 11:20

... PFU/cell Circular and square symbols represent quantities of DNA from RC2620 and the repaired virus respectively while solid and open symbols represent DNA isolated from HEL cells and HL114 cells ... 72 hpi and images of FITC, Texas Red, and a merged are shown (D-F) All images were captured digitally and prepared in Adobe Photoshop DNA synthesis should be apparent throughout the viral DNA replication ... viral DNA synthesis and suggests that this gene product functions as part of the viral DNA replication machinery Discussion Figure in Defects DNA synthesis associated with pUL114 Defects in DNA...

Ngày tải lên: 19/06/2014, 08:20

... PFU/cell Circular and square symbols represent quantities of DNA from RC2620 and the repaired virus respectively while solid and open symbols represent DNA isolated from HEL cells and HL114 cells ... 72 hpi and images of FITC, Texas Red, and a merged are shown (D-F) All images were captured digitally and prepared in Adobe Photoshop DNA synthesis should be apparent throughout the viral DNA replication ... viral DNA synthesis and suggests that this gene product functions as part of the viral DNA replication machinery Discussion Figure in Defects DNA synthesis associated with pUL114 Defects in DNA...

Ngày tải lên: 20/06/2014, 04:20

... microfluidics device, and easy to handle after washing using a permanent magnet Figure depicts the optical absorbance spectrum of the as-prepared and dye-attached DNA- tagged Au and multilayer NWs ... attached DNA, (iv) Au/Ni/Au NWs with tagging of cy3-dye attached DNA PL images of (v) Au NWs and (vi) distinct difference of Au and Ni in Au/Ni/Au multilayer NWs Figure Measurement of dark current and ... nanowires VS, HFL and LYH participated in the design of the study and performed the optical and fluorescence analysis WSL conceived of the study, and participated in its design and coordination...

Ngày tải lên: 20/06/2014, 22:20

... would amplify genomic regions within the HC-Pro and P1 cistrons There was a lack of negative genomic strands (or very faint bands) on all stems (S): the sugarcane, host for SCMV-CAM6 and the nonhost ... positive and negative genomic strands depending on the primer used in the reverse transcription: the reverse oligonucleotide (3’primer), for the amplification of the positive strand, and the forward ... absence of the negative amplified strands of SCMV-VER1 and SCMV-CAM6 in maize (L3 and L5) and sugarcane (L1 and L3), in the inoculated and systemic leaves, respectively SCMV-susceptible (SL2)...

Ngày tải lên: 11/08/2014, 21:21

... the HC-II test First, double stranded DNA was denatured by using a strong alkaline denaturation solution and converted into single stranded DNA (ssDNA) This ssDNA was then hybridized in-solution ... molecular weight genomic DNA was extracted from oral biopsies of patients by using the Qiagen QIAamp DNA tissue Kit (Qiagen Inc USA) The extracted genomic DNA was quantified and checked for purity ... (HC-II) and approved by the US food and drug administration (FDA) The performance characteristics of HC-II assay and the PCR for detection of HPV DNA have been compared in cervical lesions and results...

Ngày tải lên: 12/08/2014, 01:22

... Brassicaceae, Fabaceae and Poaceae SSP promoters; (ii) Brassicaceae and Fabaceae SSP promoters have one RY motif localized in close proximity upstream of the TATA-box, and one additional RY motif and one ... (a) Brassicaceae, (b) Fabaceae and (c) Poaceae are mapped onto promoter sequences (Motifs in Brassicaceae (B1-3), Fabaceae (F1-5) and Poaceae (P1-P7), respectively) genes and the top-ten scoring ... a seed length of six and matches on both strands [7] For motif discovery in Brassicaceae, we used a background model based on Arabidopsis promoters, for Fabaceae we used a background model based...

Ngày tải lên: 12/08/2014, 03:21

... [4,43] and glossy 15 in maize [44] Aberrant expression of miR156 and miR172 in plants disrupts normal leaf and flower development Compared with miR156 and miR172, the expression levels of miR157 and ... (miR156, miR157, miR162, miR172, and miR396) and peanutspecific miRNAs (miRn1, miRn2 and miRn2*, miRn3, and miRn4) were validated using qRT-PCR (Table 3) The primer and probe sequences for the conserved ... technique to validate and measure the expression of novel miRNAs (miRn1, miRn2 and miRn2*, miRn3, and miRn4) as well as conserved miRNAs (miR156, miR157, miR162, miR172, and miR396) All of these...

Ngày tải lên: 12/08/2014, 03:21

... distinct groups - group I and II Group I consisted of PVM isolates detected and characterized in Italy, Germany, China, Poland and Russia and group II consisted of Canadian and US isolates Isolates ... further divided into two sub-groups - Ia, and Ib PVM isolates from China and Poland formed the group Ia and group Ib consisted of isolates from Italy, Germany and Russia Isolates in group II could ... sub-groups - IIa and IIb PVM isolates Idaho and Ca128 were considered to represent IIa and IIb, respectively Group IIa consisted of isolates Idaho, Ca508, Ca513, CL1 and CL3 and group IIb consisted...

Ngày tải lên: 12/08/2014, 04:21

... online tool [31,44] The maximum depth and probability cutoff parameters were Dmax = and pc = 0.01 in Figs 3, 4, 5, 6, Dmax = and pc = 0.02 in Figure 7, and Dmax = and pc = 0.0001 in Figs 8, 9, 10 ... probability functions [Eqs (1), (2), (5), and (6)] Using Eq (1), a binary tree Ψx and a set of 1D peaks Px is computed, and using Eq (2), a binary tree Ψy and a set of 1D peaks Py is computed The ... D(a')

Ngày tải lên: 12/08/2014, 17:20