genetics of host range in lepidoptera

... (14) complexity of the sumoundings (indicating the degree of simplicity of the orography of the surrounding area; Blanco et al, 1989), (15) wind protection coefficient (according to the fluid ... previously been summarized in orthogonal components by an ordination method (see Escudero et al, 1994), since of the limitations of COR lies in the number of variables in each of the two data sets, ... Silvicultural prescriptions to sustain HF in Extremadura may result in the long-term in a loss of diversity, in spite of a high and temporally short peak of species richness following management activities...

Ngày tải lên: 08/08/2014, 18:22

... boxes (four in G1 and ten in G2) hosted snails from only three (Gl) or two (G2) families, to obtain full-sib matings and to study inbreeding effects on adult weight and age However, offspring from ... from those matings were not used for reproduction Frequencies of the different types of matings and egg-layings obtained in each generation are shown in table I Growth As room was lacking to raise ... of its offspring Since there is no contact between the layer and its offspring, it is likely that the effect is passed on through the eggs Bride and Gomot (1991) showed that, often in pairs of...

Ngày tải lên: 09/08/2014, 18:22

... sequence to Domain B The later domain may have a host- anchoring function A worm algorithm representation of the model is shown in Fig 11 Domain A, the larger of the two domains, includes residues ... supergroup A tailspike protein with endosialidase activity is implicated in allowing a specific enteroinvasive E.coli host range Insertion of such an endosialidase gene into a nonpolyvalent virulent ... for the welfare of both man and animals Our results may inform new ways of genetic manipulation of phages to alter their host binding profile Materials and methods Purification of phage particles...

Ngày tải lên: 12/08/2014, 01:22

... chromodomain implicated in chromatin binding, and a catalytic domain involved in acylation reactions (Lahn and Page, 1997; 1999b) The predicted coding regions of CDY1 and CDY2 were 98% identical in amino ... method in isolating genes that are found within the AZF locus and the euchromatic region of Yq This study, in predominantly ethnic Chinese patients in Singapore, defines the subgroup at risk of Y ... Treatment of Infertility, with the objective of creating a standard protocol for the investigation of infertile couples Normal semen was classified as containing a sperm concentration of at least...

Ngày tải lên: 16/09/2015, 15:53

... Mutations  in TRPC6  resulted in a gain of function.  Mutations in WT1 affect the DNA‐binding affinity of WT1  to the target gene. Heterozygous de novo mutations in WT1 resulting in the inability of ... C‐terminal  domain of podocin (Huber et al., 2001). The phosphorylation of nephrin also increases  the binding of nephrin and podocin (Li et al., 2004).     TRPC6  TRPC6  is  a  member  of the  ... transduction(Huber et al., 2003b).  Podocin has been found to increase the efficiency of nephrin signaling without the recruitment of other signaling molecules. The cytoplasmic  tail  of nephrin  binds  to  podocin  and  this  interaction ...

Ngày tải lên: 07/10/2015, 10:02

... glomerulonephritis.GN: Glomerulonephritis. MCNS: Minimal change nephrotic syndrome.   *Patient progressed to end‐stage renal disease. **Patient is resistant to calcineurin inhibitor.                              151 Table 2: Genotyping profile of the significant SNPs for the patients.  ... pT (p.Ala22Val)    ... FSGS with a cellular crescent  MCNS  FGS  FGS   Not performed  Glomerular minor  abnormalities  MCNS  IgM nephropathy, MCNS  Minimal change. Findings  Poor  Prognosis  No  No  Steroid  Resistant  No  No  On CNI ...

Ngày tải lên: 12/10/2015, 17:35

... important roles in interaction with its host Point mutations in the putative calcium-binding site (CBS) or hinge connecting shell and protruding domains of TCV CP appear to alter virus-ion interactions, ... 106 against virion antibody at X 105 in detection of TCV in infected N benthamiana Lanes to 10 contained equal loading of total protein of N benthamiana M: BenchMark® Pre-tained Protein Ladder ... affecting interactions between CP and plant hosts (Lin and Heaton, 1999) Moreover, Nterminus of the TCV CP was shown to be involved in eliciting resistant responses in Di-17 Arabidopsis, suggesting...

Ngày tải lên: 26/11/2015, 12:14

... post infection [36] Persistence of cowpox virus DNA in infected individuals increases the likelihood of recombination during co-infection with a poxvirus-vectored vaccine Thus homologous recombination ... homologous recombination into another OPV with broad host range during mixed infection Indeed we have shown in this work that the CEV and EEV of hybrid viruses incorporated the transgenic protein on their ... lines The stability of the influenza virus transgene inserted into MVA and hybrid progenies was assayed indirectly by monitoring the HA phenotype Serial passage of transgenic virus strains in...

Ngày tải lên: 12/08/2014, 04:21

... post infection [36] Persistence of cowpox virus DNA in infected individuals increases the likelihood of recombination during co-infection with a poxvirus-vectored vaccine Thus homologous recombination ... homologous recombination into another OPV with broad host range during mixed infection Indeed we have shown in this work that the CEV and EEV of hybrid viruses incorporated the transgenic protein on their ... lines The stability of the influenza virus transgene inserted into MVA and hybrid progenies was assayed indirectly by monitoring the HA phenotype Serial passage of transgenic virus strains in...

Ngày tải lên: 12/08/2014, 04:21

... Tseng L, Cunningham JM: Envelope-binding domain in the cationic amino acid transporter determines the host range of ecotropic murine retroviruses J Virol 1993, 67:2091-2096 Page 10 of 11 (page ... study of epizoonotic infections Conclusion We used six natural variants of the rodent XPR1 receptor to define six distinct host range types among naturally occurring X/PMVs The host range types include ... Figure infection RBD region of the deduced amino the X/PMVs used the Comparison of the viral env gene of acids sequences offor Comparison of the deduced amino acids sequences of the RBD region of...

Ngày tải lên: 12/08/2014, 23:22

... containing syncytia in response to focal areas of virus infected cells Plates were fixed and stained days later and examined for plaques of syncytia Syncytium formation and inhibitors of N-linked ... formation of multinucleated syncytium in virus infected cells, × 104 cells in six-well tissue culture plates or 105 cells in 60 mm plates were infected with virus-containing medium in the presence of ... 68:626-631 Albritton LM, Kim JW, Tseng L, Cunningham JM: Envelope-binding domain in the cationic amino acid transporter determines the host range of ecotropic murine retroviruses J Virol 1993, 67:2091-2096...

Ngày tải lên: 13/08/2014, 06:20

... rash and itching may occur; the skin may become scaly; the skin may heal by scarring; hair loss may accompany the skin injury Look for dry eyes: no tears, constant rubbing and blinking; sensitivity ... cells can initiate a response against autoantigens leading to B-cell hyperre­ activity and production of autoantibodies, causing targetorgan damage, including skin, mucosa, eyes, joints, and ... 2003) Once GVHD is initiated, T-cells produce additional proinflammatory cytokines, including tumor necrosis fac­ tor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-2, which in turn attract more...

Ngày tải lên: 12/02/2014, 19:20

... An account of the Chalcidoidea (Hymenoptera) parasitising leafmining insects of deciduous trees in Britain Biol J Linnean Soc 6: 289–335 Askew, R.R 1994 Parasitoids of leaf mining Lepidoptera: ... species of this genus Regarding the effect of taxa sampling on the reconstruction of ancestral host use, most of our species are European, reflecting the most detailed host data, but we included ... communication) Incorporation of a wider range of species into the phylogenetic and brood composition data sets would allow further testing of the number and pattern of changes in brood production...

Ngày tải lên: 14/03/2014, 16:20

... contains a putative coding region of 1059 bp, encoding a 353 amino-acid protein with a theoretical molecular mass of 41 400 Da and an isoelectric point of 5.35, as determined using MWCALC (Infobiogen) ... Subcloning in pZERO (Invitrogen) using EcoRI and BamHI resulted in loss of a part of the amplified product due to a BamHI internal site So, cloning was then performed using a TA vector from Invitrogen, ... typical of the Ó FEBS 2002 A lepidopteran Gq protein a subunit (Eur J Biochem 269) 2137 Fig Alignment using CLUSTAL W of G protein a subunit of the q family from different species, including invertebrates...

Ngày tải lên: 17/03/2014, 23:20

... Bank of the Chinese Academy of Science (Shanghai, China) and maintained in 5% CO2 at 37°C in Dulbecco’s minimum essential medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin ... markers of liver and kidney and the histological examination of hematoxylin and eosin stained major organs (Figure 5) Taking all these data into consideration, it appears that combination therapy of ... highly active in murine tumor cells, but quiescent in normal murine cells and tissues These findings indicated that hTERT promoter should be useful for targeting the pharmaceutical effects of a therapeutic...

Ngày tải lên: 18/06/2014, 19:20

... Bank of the Chinese Academy of Science (Shanghai, China) and maintained in 5% CO2 at 37°C in Dulbecco’s minimum essential medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin ... markers of liver and kidney and the histological examination of hematoxylin and eosin stained major organs (Figure 5) Taking all these data into consideration, it appears that combination therapy of ... highly active in murine tumor cells, but quiescent in normal murine cells and tissues These findings indicated that hTERT promoter should be useful for targeting the pharmaceutical effects of a therapeutic...

Ngày tải lên: 20/06/2014, 03:20

... leucine 138 in the fusion peptide region of the F protein to an arginine (pL138R) in pHRSVFOptA2 Plasmids pBD-NFκB encoding the activation domain of NFκB fused to the GAL4 DNA binding domain under ... Dissemination of HRSV to other organs is not observed even in immunocompromised individuals Similarly, disseminated infection with bovine RSV is not observed in infected cattle In contrast, in vitro ... labeled by incubation in DMEM without L-methionine and L-cysteine containing 5% dialyzed FBS and Redivue Pro-mix in vitro cell labeling mix containing (100 µCi/ml, 1.5 mls./well) [35S]-methionine and...

Ngày tải lên: 20/06/2014, 04:20

... set D, including the dinucleotide SSRs; set T, including the trinucleotide SSRs; set C, with the chloroplast SSRs This allowed us to study the effects of the repeat type and of the mode of inheritance ... the importance of testing the power of genetic markers in detecting differentiation, and the need for assessing the statistical significance of results, in studies of population genetics, evolution ... the patterns of differentiation of single alleles may stand out against an indistinct background determined by the other alleles and loci Single-allele analyses may therefore be of some help [30]...

Ngày tải lên: 07/08/2014, 16:20

... populations in Europe [17, 20] It is bivoltine [23]; in this study its overwintering generation was associated with P tabaniformis Parasitoids of P tabaniformis in Sofia During the years of investigation, ... portion (32.5–55.6%) of overwintering larvae of P tabaniformis in Sofia No studies have been conducted on the parasitoids during the summer months, but in other habitats of the host in Bulgaria three ... Faculty of “St Kl Ohridski” University in Sofia, for identifying of the species of Ichneumonidae, Dr Z Hubenov, Institute of Zoology at the Bulgarian Academy of Sciences, for identifying of the...

Ngày tải lên: 08/08/2014, 14:21

Ngày tải lên: 09/08/2014, 01:22