g allele typing and the identi fi cation of the 14 bp fragment ins del polymorphism in 3 apos utr
Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
Ngày tải lên :
23/03/2014, 09:21
... Antisense CTGGTGTGGCCCACAGAACTCAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGAGTTCTGTGGGCCACACCAG CTGGTGTGGCCCACAGAATACAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGTATTCTGTGGGCCACACCAG CTGGTGTGGGCAGCAGAACTCAGCCATTGGATGAATATAAAG ... CTGGTGTGGGCAGCAGAACTCAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGAGTTCTGCTGCCCACACCAG CTGGTGTGGGCAGCAGAATACAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGTATTCTGCTGCCCACACCAG 2B4F33Y 2B7Y 33 L 2B7Y33F 1262 FEBS ... consisted of 50 lg of microsomal proteins for UGT2B7, UGT2B7Y33L, UGT2B7Y33F and UGT2B4 and 200 lg for UGT2B4F33Y and UGT2B4F33L in 100 mm Tris ⁄ HCl buffer (pH 7.4), 10 mm MgCl2 containing 0.02...
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Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot
Ngày tải lên :
23/03/2014, 21:20
... extracts of E coli showing the overexpression (A) and insolubility (B) of the three genes pduCDE coding for the diol dehydratase of L collinoides (A) Protein content of E coli M15[pREP4] carrying the ... synthesis of the three subunits of the protein was controlled by SDS/PAGE (Fig 1A) Despite the high level of synthesis of the three proteins at 61, 24.7 and 19.1 kDa, Fig SDS/PAGE of cell-free ... (MS/MS) of the most abundant ion The collision energy for the MS/MS scan was preset at the value of 35 % Data were analysed using the SEQUEST algorithm (version 2) incorporated with the ThermoFinnigan...
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Full-Scale Dynamic Testing And Modal Identification Of A Coupled floor Slab System
Ngày tải lên :
25/11/2016, 21:53
... 13 3708 14 18,19 35 09 12 36 08 37 06 17 34 09 35 08 36 06 33 09 10 11 35 07 12 18,19 34 08 35 06 H 36 05 13 3407 11 32 09 33 08 34 06 35 05 12 15 37 01 35 04 36 02 36 01 34 05 17 32 08 33 07 10 31 09 33 06 35 03 16 34 04 ... sufficiently small Dt and long 170 S.K Au et al / Engineering Structures 37 (2012) 167–178 TM54 31 02 31 01 31 03 3202 34 02 34 01 34 03 3502 35 01 36 03 3702 37 03 16 36 04 37 04 13 3705 14 36 07 37 07 17 36 09 ... 34 04 15 32 07 31 08 32 06 33 05 10,11 31 07 33 04 31 06 32 05 33 03 16 TM57 32 04 33 02 31 05 32 03 15 33 01 31 04 32 01 TM56 TM55 18 37 09 14 19 Fig Ambient test setup (3/ F); hollow circles-columns; mega truss...
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Full-Scale Dynamic Testing And Modal Identification Of A Coupled floor Slab System
Ngày tải lên :
09/12/2016, 23:02
... 13 3708 14 18,19 35 09 12 36 08 37 06 17 34 09 35 08 36 06 33 09 10 11 35 07 12 18,19 34 08 35 06 H 36 05 13 3407 11 32 09 33 08 34 06 35 05 12 15 37 01 35 04 36 02 36 01 34 05 17 32 08 33 07 10 31 09 33 06 35 03 16 34 04 ... sufficiently small Dt and long 170 S.K Au et al / Engineering Structures 37 (2012) 167–178 TM54 31 02 31 01 31 03 3202 34 02 34 01 34 03 3502 35 01 36 03 3702 37 03 16 36 04 37 04 13 3705 14 36 07 37 07 17 36 09 ... 34 04 15 32 07 31 08 32 06 33 05 10,11 31 07 33 04 31 06 32 05 33 03 16 TM57 32 04 33 02 31 05 32 03 15 33 01 31 04 32 01 TM56 TM55 18 37 09 14 19 Fig Ambient test setup (3/ F); hollow circles-columns; mega truss...
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Báo cáo Y học: Identification of novel membrane proteins by searching for patterns in hydropathy profiles potx
Ngày tải lên :
17/03/2014, 23:20
... Subunits of both connexins and innexins contain four transmembrane domains, and combine to form dodecamers [14, 16–19] In contrast to ligandgated channels, the four transmembrane domains of connexin and ... containing six membrane-associated domains (Fig 1B) [14] In voltagegated Na+ and Ca2+ channels the four subunits are linked together as a single protein with a series of internal repeats In the ... features for browsing and organizing the large number of profiles generated by the search algorithm RESULTS A search strategy was designed for LGICs The strategy was refined by applying it to custom...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
Ngày tải lên :
15/02/2014, 01:20
... (UUAGGG) r[ UUAGGG(UUAGUG) UUAGGG] Table Amino acid sequences of RGG1 and RGG3 RGG1 RGG3 PGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMG SAGERGGFNKPGGPMDEGPDLDLGPPVDP APKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGP ... RGG3 + + C 656 + – 587 RGG3 KGG3-2 KGG3-4 KGG3-6 KGG3-4 KGG3-6 – + + + KGG3-2 – RGG3 B A AdoMet 610 MF RGG RGG D RGG F RGG RGMD RGG F MF KGG KGG D RGG F RGG RGMD RGG F MF KGG KGG D KGG F KGG ... d(CATTCCCACCGGGACCACCAC) d(CATTCCCACCGGGACCACCACCATTCCCACCGGGACCACCAC) d(TCTCTCGGTGGCCGGGGCTCGTCGGGGTTTTGGGTCCGTCC) d[ AGGG(TTAGGG) ] d[ AGGG(TTAGGG) ]⁄ d[CCCTAA) CCCT] ( 3 d[ AGGG(TTAGTG) TTAGGGJ r...
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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identification of new components and synergies between complexes ppt
Ngày tải lên :
18/02/2014, 08:20
... coverage identi cation was carried out using the big three program included in the data acquisition XcaliburÔ Finnigan proteomex 2.0 software program Protein identi cation was performed using the ... function of new proteins is based on the GH family of the catalytic module and the modular organization of the protein, or on the identity of the catalytic domain with another characterized protein ... 220927 835 585 234 1200 736 5 121824 110588924 220928189 121802 220929 230 S-GH9-CBM3-doc S-GH5-CBM32-X2-X2-Doc S-GH9-CBM3-doc S-GH9-CBM3-doc S-GH5-doc S-GH26-doc S-GH5-doc S-GH5-doc S-doc-Chagasin 83. 3...
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc
Ngày tải lên :
19/02/2014, 12:20
... is underlined): Glu291Ala (5¢-CGG ATGGGCGGCTGCGAGCGCCCTCAAGAC -3 ) and Asp394Ala (5¢-GTGGTCACGCTCGCCGGCGAGAAC CCGTGGGAG -3 ) After mutagenesis and veri cation by DNA sequencing using a MEGABACE 1000 ... understanding concerning its bifunctionality and to establish links between this GH-57 amylopullulanase and other non pullulandegrading GH-57 and GH- 13 amylolytic enzymes (F Chang-Pi-Hin, L Greffe, ... Gln14 and Pro15 in O32462_THELI and to residues Gln16 and Pro17 in Q9Y8I8_THEHY) that might correspond to the Gln-Pro dipeptide Importantly, together with the Glu79 (O32462_THELI numbering) from...
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Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx
Ngày tải lên :
07/03/2014, 00:20
... Cellular actions of the insulin growth factor binding proteins Endocr Rev 23, 824–854 Baxter RC (2000) Insulin-like growth factor (IGF-) binding proteins: interactions with IGFs and intrinsic bioactivities ... elevation of insulin-like growth factor (IGF) binding protein-1 decreases plasma free IGF-I and muscle protein synthesis Endocrinology 144 , 39 22 39 33 Westwood M (1999) Role of insulin-like growth ... human insulin-like growth factor binding protein-5 Mol Cell Proteomics 6, 139 2 140 5 43 Hoech WG & Mukku VR (1994) Identi cation of the major sites of phosphorylation in IGF binding protein3 J Cell...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
Ngày tải lên :
07/03/2014, 00:20
... (25 ng per oocyte) together with the appropriate RNA (amounts in pg per oocyte): m2R: 1500; GIRK1F 137 S: 37 .5; GIRK1F 137 SS385C: 37 .5; GIRK1F 137 SS401C: 37 .5; GIRK1F 137 ST407C: 37 .5; GIRK1F 137 SS385CS401CT407C: ... by mutating seven S ⁄ Ts in GIRK1, including the structural determinants identi ed in the present study We suggest that also in this case the contribution of GIRK1 was masked by the indirect ... amount of protein) in the different cytosolic regions of the GIRK1 G1 C-T, C-terminus (B) Upper: Autoradiogram of GIRK1 C-terminal fragments Lower: statistics of relative specific radioactivity in the...
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Báo cáo khoa học: Two CYP17 genes in the South African Angora goat (Capra hircus) – the identification of three genotypes that differ in copy number and steroidogenic output pptx
Ngày tải lên :
07/03/2014, 06:20
... CYP17 sensor TTCTGAGCAAGGAAATTCTGTTAGAC-FL probe CYP17 anchor 640-TATTCCCTGCGCTGAAGGTGAGGA-p probe Real-time 3bHSD CTGCAAGTTCTCCAGAGTC LP (sense) Real-time 3bHSD ATTGGACTGAGCAGGAAGC 39 40 RP (antisense) ... confirming that the two ovine CYP17 sequences in GenBank (GenBank accession nos L4 033 5 and AF25 138 8) are two alleles of the same gene (Fig 3) These data reveal the novel finding that, in both the ... (20 03) Rapid detection of aneuploidy (Trisomy 21) by allele quanti cation Two CYP17 genes in the South African Angora goat 30 31 32 33 34 35 36 37 38 39 40 combined with melting curves analysis of...
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Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
Ngày tải lên :
08/03/2014, 16:20
... 5¢-TGAACCTCAGCT GGCACATCTA -3 for I282Q (reverse), 5¢-ACTGCTG GGTATGCTGGGCGT -3 for Q404H (reverse), 5¢-ACT GCTGGGTTGCCTGGGCGT -3 for Q404A (reverse) Mutations were introduced by PCR mutagenesis ... corresponding to the hPXR mutations were designed: 5¢-TGAGATGTGCCAGCTGAGGTTCA -3 for I282Q (forward), 5¢-CAACGCCCAGCATACCCAGCAGT -3 for Q404H (forward), 5¢-CAACGCCCAGGCAACCCAG CAGT -3 for Q404A ... mutagenesis in a two step reaction The pCDNA3 vector primers used were na 614 5¢-CTGCTTACTGGCTTATCGAA -3 (forward) and na1106 5¢-GGGTCAAGGAAGGCACGG -3 (reverse) The mutants were subcloned into the pCDNA3...
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Báo cáo khoa học: Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases pptx
Ngày tải lên :
16/03/2014, 04:20
... bottom of a long cleft running across the width of the molecule, dividing it into the L and R domains [30 ] The binding region of chagasin formed by the loops L2 (N29–F34), L4 (P59 G6 8) and L6 ... N-terminus) (B) Zoom -in view of the interactions of papain with loop L6 of chagasin and loop L2 of cystatin B (C) Zoom -in view of the interactions of papain with loop L4 of chagasin and the N-terminal ... (loop L2) The s angle in the chagasin–papain complex is 5.8°, indicating that the chagasin molecule is slanting towards the R domain The angle of approach of cystatin B is )12.7°, and the inhibitor...
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Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
Ngày tải lên :
16/03/2014, 18:20
... (5¢-ATCGTCGGG CTCAGGATCCTTAGTGATGGTGATGGTGATGAGA TGAAGATGAAGCTGAAGA -3 ), for Gas15 23- H, or His-XH-Sdown (5¢-GTCGTCGAGCTCAGGATCCTTA GTGATGGTGATGGTGATGATCAACACTACCTGAT GCAGA -3 ), for sGas1482-H (the ... Gas1523p (lacking the C-terminal GPI-attachment signal) and Gas1482p (also lacking the Serbox region) – were obtained using the forward primer XHup (5¢-GCATATTCGACTGACTCGAGACGATGT TCCAGCGATTGAA -3 ) ... (5¢-GCATATTCGACTGACTCGAGACGATGT TCCAGCGATTGAA -3 ) and the reverse primers XHdown (5¢-ATCGTCGGGCTCAGGATCCTTAAGATGAAGA TGAAGCTGAAGA -3 ) or XH-Sdown (5¢-GTCGTCG AGCTCAGGATCCTTAATCAACACTACCTGATGC AGA -3 ), respectively XHup...
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Báo cáo khoa học: Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom Identification of the origin of a new viper population based on ammodytin I1 heterogeneity docx
Ngày tải lên :
17/03/2014, 03:20
... GACCGgtaag/tccagCTGCT CTGTGgtgag/tgcagGAGGC (110) AGGCGgtgag/tccagTTGAA GACCGgtaag/tccagCTGCT GTGCGgtgag/tgtagGAGAC (110) AGGCGgtgag/tttagTTGAG GACCGgtaag/tccagCTGCT CTGCGgtgag/tgcagGAAAA (110) ... CAGGAAACAGCTATGAC CGGAATTCTGAAGGTGGCCCGCC AGGTGACAG CGCGGATCCAATCTTGATGGGGC AGCCGGAGAGG AGGAYTCTCTGGATAGTGG CTCACCACAGACGATWTCC CGGTAAGCCCATAACGCCCA CAGGCCAGGATTTGCAGCC CATAAACAYGAGCCAGTTGCC GAGTGGATGCACAGTCGTTG ... 1 63 241–2 43 667–674 239 CAGCTgtaag/tccagGTCTG A B C D 1 63 2 43 671 261 CAGCTgtaag/tccagGTCTG GGGCGgtgag/tccagTTGAA AGGCGgtgag/caaagCTGAA GACCGgtaag/tccagCTGCT CTGTGgtgag/tgcagGAGGC (110) GACCGgtaag/tccagCTGCT...
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Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
Ngày tải lên :
17/03/2014, 17:20
... 15 63 (1562) Da 2 733 (2 732 ) Da 5122 (5120) Da 1 533 (1 533 ) Da 2705 (27 03) Da 5094 (5091) Da 31 7DFMTLTAESGAIGGVPAGGVRFGASYNA344 286GAIELEKDVAVNLGVGAPEYVASVADEEG IVDFMTLTAESGAIGGVPAGGVR 337 Despite the ... topology is found in both subunits and differs considerably from other a helix/b sheet proteins including nucleotide-binding domains In this communication, we report the cloning and sequencing of the ... YIAGHWATVPALGK GTYADESGNITFEKEVAPLEGTSV-QA Antisense: Not used 5¢-ATGMGNAARGTNCCNATHATHACN GCNGAYGCTGC -3 5¢)CCNARNGCNGGNCANATNGCC -3 37 6 T Selmer et al (Eur J Biochem 269) Ó FEBS 2002 Fig Sequence...
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Báo cáo Y học: Identification of mammalian-type transglutaminase in Physarum polycephalum Evidence from the cDNA sequence and involvement of GTP in the regulation of transamidating activity potx
Ngày tải lên :
17/03/2014, 23:20
... with gene-specific primers: 5¢-GGTC ATTCAGTCGATCGATTTAC -3 (sense, 616– 638 ) and 5¢-TGGAACAACTGGAACGGGTGCTG -3 (antisense, 522–544) For the nested PCR, the primers 5¢-CAAGTCG AGAAGAATAGAGC -3 (sense, ... hydrolysis by TGase 2, TGase 3, and PpTGase GTP-hydrolysing activities of guinea pig TGase 2, mouse TGase 3, and PpTGase were determined using lg, lg, and lg proteins, respectively The reaction ... fusion protein as an antigen PCR was performed using the PpTGase cDNA as a template with primers 5¢-CGGGATCCATATGGGACC CGTGCCTATTTCTGCT -3 and 5¢-CCGGATCCTTAA ACGACAATAACTTGGGCTTG -3 The PCR product...
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Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
Ngày tải lên :
23/03/2014, 13:20
... 5¢-GGTTAGGGTTAGGGTTAG -3 5¢-GTTAGGGTTAGGGTTAGG -3 5¢-CCTAACCCTAACCCTAAC -3 5¢-GUUAGGGUUAGGGUUAGG -3 5¢-GTTGGGGTTGGGGTTGG -3 5¢-AATTAACCCTCACTAAAGGG -3 5¢-GTAATACGACTCACTATAGGG -3 5¢- AATCCGTCGAGCAGAGTT -3 ... Tel3 Tel4 Tel5 Tel6 Tel6-Rev Tel6RNA Tet-tel T3 T7 TS OvhF OvhR CSB1 5¢ biotinTel6 5¢-TTAGGGTTAGGGTTAGGG -3 5¢-TAGGGTTAGGGTTAGGGT -3 5¢-AGGGTTAGGGTTAGGGTT -3 5¢-GGGTTAGGGTTAGGGTTA -3 5¢-GGTTAGGGTTAGGGTTAG -3 ... 5¢-CTGGCCGTCGTTTTACTTAGGGTTAGGGTT AGG -3 5¢- GTAAAACGACGGCCAG -3 5¢-GTACAGTGTACAGTGTACAGT -3 5¢ biotin-GTAATACGACTCGTTAGGGTTAGGGT TAGG -3 0.9, 2.25, 4.5, 9, 18 and 36 pmol As the order of addition...
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Báo cáo khoa học: Isolation and identification of antimicrobial components from the epidermal mucus of Atlantic cod (Gadus morhua) docx
Ngày tải lên :
23/03/2014, 15:21
... monitored against E coli including medium E in the agarose The height of columns represents the magnitude of antimicrobial activity and can be read on the right Y axis scale in mm The initial step ... 50 in Fig 3A) was active against both E coli and B megaterium This component was purified by elution at 37 % acetonitrile in the initial RP-HPLC step (fraction 25 in Fig 3B) and at 42% in the final ... outdoor seawater aquarium at the Marine Research Institute of Iceland and 50 specimens were caught randomly After killing the fish by a concussion of the brain by striking of the cranium, mucus samples...
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Báo cáo khoa học: Identification of the N-termini of NADPH : protochlorophyllide oxidoreductase A and B from barley etioplasts (Hordeum vulgare L.) ppt
Ngày tải lên :
30/03/2014, 02:20
... time of 6.55 as in (A) the standard method by decreasing the cone voltage to 35 V and increasing the capillary voltage to 35 00 V (see Experimental procedures) Identi cation of the N-terminal amino ... proteins [25] We used acetic anhydride for in- gel acetylation of primary amino groups of the proteins and OMX-SÒ reaction tubes for efficient in- gel digestion The a-amino group at the N-termini and ... The N-terminus of mature PORA from Hordeum vulgare L was first determined by Edman degradation [ 23] , and resulted in identi cation of G7 5 as the first amino acid of the mature protein Shortly thereafter,...
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