functional approaches of developmental competence of the oocyte embryo

... interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in coordinating ... product of PRPP from the crystallization solution ing 81 compounds of substrates, products and regulators of other enzymes in the human nucleotide metabolism (Table S1) The library can then be ... conclusion can be made based on the lack of thermal shifts when only the bases were added, whereas the addition of PRPP led to a significant thermal shift Kinetic studies of PRTFDC1 showed that this...

Ngày tải lên: 15/02/2014, 01:20

... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III proteins The ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the ... [14] The side-chains of the first pair of residues, i.e K135 and R148, matched almost exactly; those of the second pair were in close proximity The R148 residue of the Pfu-primase is part of a...

Ngày tải lên: 18/02/2014, 18:20

... that the basic region, particularly the conserved Arg65, but not His57, is essential for the suppressive activity of DEC1 Determination of the binding domain of DEC1 to BMAL1 The interaction of ... interaction of 4418 F Sato et al (Eur J Biochem 271) DEC1 with BMAL1 and the binding to the E-box Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the ... ng of each) Transfection of 0.1 ng of the DEC1 expression vector did not significantly decrease the transcriptional activity of CLOCK/BMAL1 (Fig 1B) Transfection of ng of the expression vector resulted...

Ngày tải lên: 19/02/2014, 16:20

... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs [19], which is reminiscent of the structure of ... profoundly the activity of the protein However, interactions with helix could play an important functional role, such as the correct positioning of the SAP motifs in relation to the rest of the ... centre of the junctions [19] Detailed understanding of the functional role of these interactions would require the crystal structure of the Ydc2–junction complex In this study the function of several...

Ngày tải lên: 20/02/2014, 11:20

... On the basis of the results with antibodies, a series of PrP mutants were made to assess whether deletions of certain domains of the protein decrease the SOD-like activity of the protein The ... assay The results of these experiments are shown in Fig Several of the antibodies and antisera caused a concentrationdependent inhibition of the SOD-like activity of PrP The ability of the antibodies ... to the conformation of the C terminus indicated that the conformation of the C terminus is important to the activity of the protein Deletion of the whole C terminus rendered the protein inactive...

Ngày tải lên: 21/02/2014, 00:20

... illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top of the hollow (Fig 1A) The ... the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain ... established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL...

Ngày tải lên: 21/02/2014, 15:20

... rule out that the AAA peroxins also form separate subcomplexes, the presence of the components of the importomer in the precipitates of the AAA peroxins as well as the appearance of the typical ... interestingly, in contrast with the lack of other components of the peroxisomal protein import machinery, the deficiency of Pex4p does not interfere with the assembly of the components of the importomer In ... Moreover, these data indicate that at least one of the contact sites of the AAA complex and the importomer is made by Pex15p To investigate the influence of individual peroxins of the importomer on the...

Ngày tải lên: 07/03/2014, 12:20

... the binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP-binding to the two AAA-cassettes of Pex1p and Pex6p for their interaction The ... comparable to that of the full-length Pex1p (Fig 1A) These findings indicate that binding of Pex6p is best in the presence of both AAA-cassettes of Pex1p However, the data also indicate that the first AAA-cassette ... additional presence of either the N-terminal fragment or more drastically by the presence of the second AAA-cassette This is consistent with the observation that a mutation in the beginning of Pex1pD2...

Ngày tải lên: 07/03/2014, 16:20

... glycosaminoglycans [37] As the myc-his tag of the protein was present at the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was ... respectively Purification of CCR5-myc-his For the purification of CCR5-myc-his we tested two different methods based on the presence of the six histidines at the C-terminus of the protein The first involved ... One of the consequences of signalling is a down-modulation of the expression of CCR5 [38] CCR5-myc-his or wtCCR5 expressing cells were incubated at 37 °C for 30 with 10 nM of MIP-1a and then the...

Ngày tải lên: 08/03/2014, 09:20

... Analysis of the phase of each of the xMGP introns located within the coding region revealed that introns and are of phase I while intron is of phase II [29] The same phases are found in the corresponding ... this study, we present the molecular organization of the rst nonmammalian MGP gene and the functional analysis of its promoter We identied a region within the rst 70 bp of the xMGP promoter that ... representation of the xMGP promoter constructs used for transient transfections of A6 cells is shown to the left The nomenclature of the promoter deletions is based on the transcription start of the xMGP...

Ngày tải lên: 08/03/2014, 10:20

... of protons attached to the basic sites of the amino-acid sequence The m/z values for the ions have the general form [M + zH]/z, where z equals the number of protons attached It follows that the ... typical positive ion spectrum of a single protein consists of a series of peaks, each of which represents a multiply charged ion of the intact Injection of the total mixture of phycobilisomes from Synechocystis ... differ from each other by the percentage and type of bilin proteins they contain In order to study the influence of certain environmental changes on the supramolecular organization of the 1538 L Zolla...

Ngày tải lên: 08/03/2014, 16:20

... features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic properties of the mutants, and simulations of the substrate–enzyme interactions of the wild-type ... analysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding The amino acid sequence of Zm-p60.1 was compared with the ... in the wild-type, whereas the proportions of a helices appear to be lower in F461L, F200K and P2 Furthermore, the F193A and W373K mutations not result in any change in the thermostability of the...

Ngày tải lên: 16/03/2014, 04:20

... the presence of the nucleotide These experiments indicate that the binding and/or hydrolysis of ATP not have any effect on the conformation of PfPDO, possibly because of the localization of the ... participation of both N-terminal and C-terminal active sites Refolding of the scrambled RNase in the presence of PDI was used as a positive control, and the absence of the recovery of the RNase activity ... using PDI However, the temperature of the assay, which is limited by the stability of the protein substrate RNase, is very far from the optimal growth temperature of the hyperthermopilic micro-organism...

Ngày tải lên: 16/03/2014, 16:20

... The amplitude of the fast phase of the latter represented up to 40% of the total amplitude, with a rate constant (kobs) independent of Pc concentration (data not shown) From the kobs values of ... spinach Pc/fern PSI system are of the same order of magnitude as those observed for the spinach Pc/PSI system [15], the distance and/or orientation of the redox centers in the fern Pc/PSI complex seems ... extend the functional characterization of fern Pc, we have also compared the reactivity of this protein with that of other Pcs towards several soluble c-type Cyts (including Cyt f) by using the...

Ngày tải lên: 16/03/2014, 16:20

... in cells of the inner hypodermis and the epithelium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms Discussion A bundle of four a-helices constitutes the secondary ... staining of the pseudocoel (ps) especially in the vicinity of the uterus (ut) with developing eggs (C) Strong labeling of the oviduct (ovi) next to the unstained ovary (ov) in A suum (D) Section of the ... determined by the mutual spatial orientation of the transition dipole moments of the donor and acceptor As no data on the spatial orientation of the transition dipole moments of the chromophores...

Ngày tải lên: 16/03/2014, 18:20

... retrieved from the Golgi and the underglycosylation of proteins normally modified in the Golgi [10] Neither the precise function of ERD1 nor the mechanism of ERS1mediated suppression of erd1D is ... with which these CTNS mutant alleles can complement ers1D Importantly, the inability of these mutant ctns alleles to complement ers1D provides the proof of principle for the utility of yeast as ... suppression of ers1D by MEH1, and the role of Meh1 and Gtr1 in regulating vacuolar function The results we have presented validate the utility of yeast as a model system for the functional analysis of...

Ngày tải lên: 16/03/2014, 18:20

... significantly in the presence or absence of the b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b1 subunit The effects of b1B were further assessed ... that the intracellular segment of the b1 subunit is required for the interaction with the a subunit, probably a crucial step for the regulation of channel properties The tissue distribution of the ... currents for the number of oocytes indicated (G) Effect of b1B on the current amplitude of NaV1.2 Each individual data point in the histogram represents the peak inward current for a single oocyte Also...

Ngày tải lên: 16/03/2014, 23:20

... estimated from Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined ... catalyse the synthesis and insertion of a cytidine dinucleotide cofactor into recombinant Qor as suggested by the release of CMP after hydrolysis of the enzyme, it appears that the assembly of intact ... microwave power of mW Trace B is multiplied by a factor of six to show the small signals of the rapid species in this sample The arrows indicate the position of contribution of the resting species...

Ngày tải lên: 17/03/2014, 10:20

... Analysis of the 5¢ flanking region sequence of the Bsep gene A total of 2488 bp upstream from the 5¢-end of the Bsep cDNA was sequenced in both directions; 1583 bp are shown in Fig Identity with the ... even below that of the wild-type p-126 Luc Analysis of the binding affinity of the putative FXRE site for the orphan nuclear receptor FXR To characterize the FXRE site identified in the Bsep promoter ... Thus, we present the nucleotide sequence, as well as a systematic structural and functional analysis of the 5¢ flanking region of the Bsep gene The 5¢ deletional analysis of the Bsep promoter...

Ngày tải lên: 17/03/2014, 23:20

... of the extracellular part of the receptor [28] Therefore, analysis of the ability of the mutants gp130D4 and gp130D4 to form complexes with the LIFR by coprecipitation was not possible with the ... compensate for the shift of the ligand-binding epitopes of the receptors The domain architecture of GCSFR is identical to that of gp130; moreover these receptors share 46% sequence homology The gp130 ... to the CBM that play specific roles in activation of the receptor by these two cytokines The ligand independent activation of the D5-GCSFR chimera occurs in both the absence and the presence of...

Ngày tải lên: 18/03/2014, 01:20