function constant term of eisenstein series and residual spectrum

... 28 Figure 3-2: Morphology of STMN1 KD and SC cells 29 Figure 3-3: Morphology of STMN1 OE and vector control cells 30 Figure 3-4: Panel of stable STMN1 KD and OE cell lines 31 Figure ... accessibility of barium sulfate to early lesions and three-dimensional reconstruction of the inner colon surface Since these often still require verification by standard colonoscopy, the utility of virtual ... STMN1-mediated dissolution of the mitotic spindle and reconstitution of the interphase MTs57 Consistent with the critical role of STMN1 in MT regulation, both high and low expressions of STMN1 were reported...

Ngày tải lên: 09/09/2015, 11:25

... binding determinants in the C-terminus of hOR2AG1 by spotting peptides that correspond to mutated or shortened receptor C-termini Truncation of the last amino acids abolished binding of the C-terminus ... interaction partner of olfactory receptors and have elucidated the molecular details of this interaction 7286 The primary source of olfactory signaling and the sites of expression of ORs are the ciliary ... 3¢ primers and pCDNA3_OR2AG1 as a template GST fusion constructs of the C-terminus of OR2AG1, and mutant thereof (trunc8) were created by cloning the region between amino acids 293 and 316 from...

Ngày tải lên: 18/02/2014, 13:20

... and A8B8-GAPDH We examined the effect of these modifications on the enzyme activities, their reversibility in the presence of reductants, and the protective effect of the substrate BPGA and cofactors ... against oxidative damage of chloroplastic A4-GAPDH Results Inactivation of A4-GAPDH by GSSG and other oxidants, and protection by substrate and cofactors Incubation of recombinant Arabidopsis ... Inactivation of A4-GAPDH in the presence of H2O2 and GSH (A) Protective effect of NADPH and BPGA A4-GAPDH was incubated with 0.1 mM H2O2 and 0.5 mM GSH alone (open triangles), or in the presence of 0.2...

Ngày tải lên: 19/02/2014, 05:20

... the b11-strand of the C-terminal tail of NBD2 is necessary and sufficient to confer the different nucleotide binding and functional properties of SUR2A and 2B [39] As the sequence of the TAP-NBD2 ... Chimera 1C2 encoded residues 1–505 and 653–725 of TAP1 and residues 494–639 of TAP2 and chimera 2C1 encoded residues 1–493 and 640–703 of TAP2 and residues 506–652 of TAP1 The exchange was performed ... that of wild-type TAP and of the original nonfunctional TAP variants 1–2N1 and 2–1N2 (Fig 3A, top) and showed normal subunit assembly (Fig 3A, bottom) We measured the peptide transport function of...

Ngày tải lên: 20/02/2014, 02:21

... Phosphorimager (+ and ++ in the case of TFIIIB represents and lg of protein) The quantification of the transcripts (done in Phosphorimager) in each of the lanes are shown on the right-hand side of the panels ... PC-B and PC-C as well as with the heparin–Sepharose fractions The reactions were maximally active at lg of both PC-B and PC-C (Fig 2B; lane 4) and at lg of TFIIIB and RNA pol III fractions (0.3 and ... and termination sites of the gene [22] Fractionation of the B mori posterior silk glands nuclear extract Transcription factors TFIIIB and TFIIIC were partially purified from posterior silk gland...

Ngày tải lên: 20/02/2014, 02:21

... Materials and methods Fig N-Terminal fragments of LBR expressed as GST fusion proteins, and the binding of beads bearing these fragments to chromatin (A) Schematic diagrams of N-terminal fragments of ... proteins The line numbered and 211 shows the N-terminal portion of the LBR molecule, and these numbers are those of amino-acid residues from the N-terminal of LBR ÔRSÕ is the site of arginine-serine ... varied binding of the N-terminal portion of LBR to chromatin is regulated through the RS region, not the NM- and SK-regions The directions of the changes in the affinity of NK and chromatin on...

Ngày tải lên: 22/02/2014, 04:20

... footprinting of the 5¢ upstream region of the a-F1-ATPase gene and found that the combination of GAF and Adf-1 binding sites is present both in other Drosophila species (D yakuba and D pseudoobscura) and ... of the gene (a-RT1 and a-RT2; see Materials and methods) and an oligo(dT) primer With the combination of oligo(dT)/a-RT1 primers two 700 and 320 bp fragments were amplified both from embryos and ... was mutated and the activity of the mutant constructs was assessed Wild type and mutated GAGA and Adf-1 binding sites are shown by open and filled symbols, respectively (C) Activation of the a-F1-ATPase...

Ngày tải lên: 07/03/2014, 16:20

... aggregates offers new possibilities in the investigation of the kinetic parameters and mechanism of violaxanthin de-epoxidation One of the advantages of such system is the defined orientation of violaxanthin ... parameters of violaxanthin into zeaxanthin conversion and flip-flop rate of antheraxanthin A possible molecular mechanism of violaxanthin de-epoxidation is proposed MATERIALS AND METHODS Preparation of ... buffer (pH 5.1) In another series of experiments, liposomes with constant concentration of monogalactosyldiacylglycerol (12.9 lM) and violaxanthin (0.33 lM) were used and PtdCho was changed in...

Ngày tải lên: 08/03/2014, 10:20

... found in cells of the gland ducts and mucous glands [9] In the adult lung, the expression of MUC5B follows a restricted pattern, with a positive gradient from the surface to the glands and a decrease ... of )478 ⁄ )1 with )478 ⁄ +47 and of )717 ⁄ +47 with )747 ⁄ )1) TTF-1 is a strong repressor of Muc5b expression Overexpression of TTF-1 in CMT-93 cells led to a strong decrease in the amount of ... 10) and cold T84 (lane 16); supershift analysis with anti-GATA-4 (lanes 5, 11 and 17), anti-GATA-5 (lanes 6, 12 and 18) and anti-GATA-6 (lane 7, 13 and 19) IgGs The DNA–protein complex (GATA) and...

Ngày tải lên: 15/03/2014, 00:20

... conformation with the N-terminus folded on the C-terminus and that ICAP-1 binding to the N-terminal part disrupts Krit1 N- and C-termini interaction Fig Krit1 N- and C-terminal parts associate ... analyses of these residues will help to determine whether the binding site of the polar head of PIP2 on Krit1 is similar to that of radixin Many members of the FERM family undergo intra and ⁄ or ... inter molecular folding of their C-terminus onto their N-terminal FERM domain [21] Consistently, we demonstrate the interaction of the N-terminus of Krit1 with its C-terminus, with both parts...

Ngày tải lên: 16/03/2014, 05:20

... variant of the most recently discovered PDE family [13–16] The four PDE11 isoforms differ in terms of the length of their N-terminal domains Only PDE11A4 contains a complete N-terminal tandem GAF ... the tandem GAF domain of PDE10, and by cGMP when linked to the tandem GAF domain of PDE2, )5 or )11 The biochemical properties of signalling by human tandem GAF domains to cyaB1 AC were by and ... N-terminus of the PDE11A4 GAF tandem may constitute a mechanism regulating the cGMP affinity of the PDE11A4 tandem GAF domain and thus allosterically affect PDE11 activity The role of the N-terminus...

Ngày tải lên: 16/03/2014, 06:20

... catalytic domain of p25rum1 is located in its C-terminal portion and deletion of 74 N-terminal amino acids does not affect the activity of p25rum1 as an inhibitor of Cdc2 kinase Effect of N-terminal ... Phosphorylation of wild-type and A13A19 mutant of p25rum1 by MAPK (A) GST fusion proteins of wild-type (WT) (lanes and 3), the A13 deletion mutant (lane 2), and the A19 mutant (lane 4) of p25rum1 ... was examined in the presence or absence (lane 8) of GST fusion proteins of wild-type (lane 2) and C- and N-terminal deletion mutants (lanes 1, 3–6) of p25rum1, as indicated In lane 7, GST protein...

Ngày tải lên: 17/03/2014, 23:20

... of the tail of injected shrimps (Fig 9D and E) Confrontation of haemocytes and bacteria The recruitment of penaeidin-positive haemocytes around the site of injection confirmed the importance of ... site of microbial injection precluded both clarification of the mechanisms of penaeidin release from haemocytes, and determination of any potential involvement of penaeidin in the elimination of ... decrease in numbers of both circulating and tissue infiltrating haemocytes within h of injection To study haemocyte behaviour and changes, sections of the last abdominal segments (site of injection)...

Ngày tải lên: 18/03/2014, 01:20

... Innotech, San Leandro, CA, USA) The relationship between the inverse of band intensity and the number of PCR cycles was linear The number of PCR cycles was 45 for ATF3, AdipoR2 and GAPDH Western ... and/ or depletion of calcium ions [6–8], and this perturbation of ER homeostasis occurs in diet-induced and genetic models of obesity [9] Studies of the regulation of obesity-linked insulin resistance ... AdipoR2 Fig Changes in expression of AdipoR2 under conditions of ER stress and ATF3 over-expression (A) Relative levels of GRP78, ATF3 and AdipoR2 protein in C57BL6/J and ob/ob mice (n = for each...

Ngày tải lên: 22/03/2014, 21:21

... intensity of both bands and normalizing them by adding both bands and taking the fractional intensity belonging to the particular (substrate, product) band Such measured intensity of bands was ... (BioRad) and the image and the band intensities measured and quantified with a BioRad PhosphoImager FX and quantity one software All enzyme kinetic experiments were repeated in triplicate and Kobs ... solutions of known concentration of phosphorus and sulfur and other elements for references, in parts per billion concentration The absolute concentration of sulfur and phosphorus was determined...

Ngày tải lên: 23/03/2014, 03:20

... the effect of cAMP signaling on SUMO pathway is complex and cell type dependent Notwithstanding, the up regulation of E1, the down regulation of E2 or E3 and the increased expression of SENP2 lead ... incubating lL of in vitro 35S-labeled product with lg of Aos1 ⁄ Uba2, 150 ng of UBC9 and lg of SUMO-1 in sumoylation buffer (20 mm Hepes, pH 7.5, mm MgCl2 and mm ATP) SAE1 ⁄ SAE2, UBC9 and SUMO-1 ... lL of serum-free medium were placed in a mm cuvette (Bio-Rad, Hercules, CA, USA) A total amount of lg of DNA (2 lg of Myc-tagged LRH-1 expression plasmid, 1.5 lg of pDsRed-SUMO-1 and 1.5 lg of...

Ngày tải lên: 23/03/2014, 06:20

... 20 lL of the cell lysate was mixed with 20 lL of reagent (A + S), and 160 lL of reagent B was then added Absorbance was measured at 750 nm, and compared to the absorbance of dilutions of a protein ... approximatively half (53 ± 2%) of the amount of cadmium found in cells grown in the presence of cadmium is present in cells grown in the presence of emetine and cadmium Thus, Q = · QE, and Ro · t = 2Ro · ... corresponds to the measurement of loss of cell integrity following total lysis of the cells with detergent (C) Graphical representation of the phenotypic modification of cells from the experiment...

Ngày tải lên: 23/03/2014, 06:20

... Proteasome-dependent cleavage of Suc-LLVY-NH2-Mec constituted up to 95% of total cleavage in untreated cells, and 90 and 70% of total cleavage in cells treated with PP1 for 72 and 96 h, respectively ... (average of triplicates) Shown is a representative of three independent experiments organisms resulted in stabilization of specific groups of proteins [25,59–61], and overexpression of S5a induced ... in frame with a N-terminus (HA)3 tag in vector pcDNA3 Expression of this construct was measured in Saos-2 and HeLa tet-off cells Saos-2 (1.7 · 106 cells per plate) and HeLa tet-off cells (2 · 106...

Ngày tải lên: 23/03/2014, 09:20

... normal tissues and cancers To determine the distribution of this putative MBP-1 transcript in normal tissues and cancer cells, commercially available cDNAs of a variety of normal tissues, and the cDNAs ... Expression and regulation of MBP-1 A A B Fig Abundance of putative MBP-1 and ENO1 transcripts in various tissues and cell lines (A) The ENO1 and putative MBP-1 transcript levels were determined ... cytoplasmic (C) and nuclear (N) fractions of BEAS-2B and A549 cells were fractionated as described in the Materials and methods Western blot analysis of one-tenth of the original volume of each fractionated...

Ngày tải lên: 29/03/2014, 21:20

... study of the mechanism of action of insulin on human skeletal muscle and liver glycogen synthesis and the pathophysiology of human type diabetes Materials and methods Amplification of PPP1R3E and ... Subcloning and sequencing of the PCR products confirmed the identity of the testis and brain cDNAs for PPP1R3E and showed that the protein translated from this sequence was composed of 279 amino ... coding exon specifying a protein of 358 amino acids and a molecular mass of 38 kDa (data not shown) Comparison of the glycogen-targeting subunits of PP1 Searching mouse and rat genomic sequences in...

Ngày tải lên: 30/03/2014, 16:20