from drug discovery to quality control
modern HPLC for practicing scientists
Ngày tải lên :
06/10/2016, 09:27
... 136 6.1.2 Overview: From Drug Discovery to Quality Control / 136 6.1.3 Sample Preparation Perspectives in Drug Product Analysis / 137 6.1.4 High-Throughput LC/MS in Drug Discovery Support / 137 ... in Absorbance Detectors / 89 Photodiode Array Detectors / 91 4.6.1 Operating Principles / 91 4.6.2 Trends in PDA Detectors / 93 Other Detectors / 93 4.7.1 Fluorescence Detector (FLD) / 93 4.7.2 ... α-tocopherol α-tocotrienol β-tocotrienol γ-tocotrienol Minutes Figure 1.4 A normal-phase HPLC chromatogram of a palm olein sample showing the separation of various isomers of vitamin E Chromatogram...
- 306
- 983
- 0
In silico methodologies for selection and prioritization of compounds in drug discovery
Ngày tải lên :
08/09/2015, 22:12
... converted into a vector of descriptors Hence the input from the library of compounds into RFC is a matrix of descriptors with each compound represented as a vector This matrix of descriptors is labelled ... years and up to US$1.3 billion from the conception phase to the market Technologies such as combinatorial chemistry 3, and high-throughput screening 5, were intended to speed up drug discovery significantly ... past few decades, drug discovery continues to suffer from low efficiency and high failure rate Hence the emphasis has been on applying approaches that are able to expedite the drug discovery cycle,...
- 185
- 380
- 0
Biointormatics of targeted therapeutics and applications in drug discovery
Ngày tải lên :
12/09/2015, 09:10
... facilitate target and drug discovery The aim of updating TTD is to make it into a more useful target and drug discovery resource in complement to other related databases such as drugbank (40) Continuous ... identified from large chemical databases A small percentage of virtual hits validated by wet-lab experiments will enter the next phase of drug discovery From hit to lead to clinical trial drugs and to ... of cancer drug development focus from the traditional cytotoxic chemotherapy to molecularly targeted therapeutics The mechanisms of action and toxicity profiles of molecularly targeted drugs are...
- 232
- 496
- 0
lc ms applications in drug development
Ngày tải lên :
11/04/2014, 07:01
... Significant events are highlighted with respect to their relationship to analysis requirements Drug Discovery The goal of the drug discovery stage is to generate a novel lead candidate with suitable ... cost of analysis from $125/sample to $25/sample (Courtesy of Milestone Development Services, Newtown, Pa., USA.) itative process approaches used to eliminate candidates from the drug development ... characterized in humans Subsequent analyses continue to be performed under strict protocol and regulatory compliance to register the drug for NDA/MAA 14 DRUG DEVELOPMENT OVERVIEW Once the NDA/MAA is...
- 255
- 298
- 0
DRUG DISCOVERY RESEARCH IN PHARMACOGNOSY ppt
Ngày tải lên :
27/06/2014, 10:20
... the information obtained from chromatographic analysis to address further the problem of quality control for herbal medicines [Liu, 1994, 1993] Phytoequivalence and chromatographic fingerprints ... between phytochemistry and medicine Phytochemistry 30, 3864-3874 22 Drug Discovery Research in Pharmacognosy [2] Mendonca-Filho, 2006 Bioactive phytocompounds: New approaches in the Phytosciences ... needs to be quality checked to ascertain that it confirms to quality requirement and delivers the properties consistently Standardization assures that products are reliable in terms of quality, ...
- 254
- 312
- 0
In silico drug discovery on computational Grids for finding novel drugs against neglected diseases
Ngày tải lên :
02/07/2014, 13:13
... resistance to existing drugs worldwide is displayed in Figure Resistance to any new therapeutic agents is expected Strategies to lengthen the drug lifetime are combination drug therapy and use of old drugs, ... important to recognize and understand that parasitic drug discovery differs from chronic drug discovery process (preventable diseases such as diabetes, cancer, cardiovascular diseases, respiratory ... rational drug discovery methods will increase the hit rate when compared to random high throughput screening approach 19 Chapter State of the art on rational drug discovery Why computer aided drug discovery...
- 183
- 286
- 0
Báo cáo y học: "Distribution patterns of small-molecule ligands in the protein universe and implications for origin of life and drug discovery" doc
Ngày tải lên :
14/08/2014, 08:20
... the discovery of a new drug is to start with an old drug [83-85] Another feature of the recent drug discovery paradigm shift is that more attention has been given to natural-product repositories ... ligands derived from sc-PDB (due to the lack of atomic parameters, 10 of the 2,186 ligands failed to go through the descriptor calculations) and 2,184 small molecules randomly selected from ACD-SC ... (derived from sc-PDB) and ordinary molecules (randomly selected from ACD-SC), defined by the first two factors Figure from 70 descriptors derived Chemical space consisting of ligands (derived from...
- 13
- 358
- 0
Pharmacokinetics pharmacodynamics driven approach for lead optimization in anti mycobacterial and anti malarial drug discovery
Ngày tải lên :
22/09/2015, 15:17
... urgently Figure History of drug discovery and development of treatment regimens for TB Reprinted from Ma et al (2010), with permission from Elsevier 1.2.2 Ideal drug candidates An ideal drug combination ... because drug- drug interactions with anti-retroviral therapy are difficult to avoid (Balganesh et al., 2008; WHO, 2012b) To make the landscape even more complex, there are recent reports of totally drug ... 1.2.1 Discovery of anti-mycobacterial drugs 1.2.2 Ideal drug candidates 1.2.3 Drug development pipeline 1.2.4 Combination therapy 1.2.5 Challenges in TB drug discovery...
- 208
- 406
- 0
Implementation of a drug discovery tool for the evaluation of anti fibrotic compounds application in fibrovascular disorders
Ngày tải lên :
09/10/2015, 11:07
... direction: top-top, top-bottom, bottombottom, bottom-top (the first referred to the excitation source point and the latter referred to the emission reading point) After optimization, top-bottom and top-top ... antifibrotic drug discovery tool Therefore, this project aims to establish a reliable cell enumeration assay that is suitable to quantify the fibroblast population and can be incorporated into the ... collagen scaffold fabricated from human fibroblasts might offer an alternative to animal-originated collagen This project also aimed to develop an anti-fibrotic drug discovery tool that allows the integration...
- 74
- 224
- 0
Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
Ngày tải lên :
16/10/2015, 15:38
... as useful chemical tools to control stem cell fate and will likely to provide new insights into stem cell biology One approach to generate functional small molecules that control stem cells fate ... colonies were injected into SCID mouse in vivo to form teratomas First observation of teratoma lumps was 23 weeks after injection Teratomas (7 weeks post injection) were excised from euthanized mice ... β-actin as control From lane to lane are expression levels of genes for hESCs H9 colonies, days EB and days EB respectively Endoderm Mesoderm 26 Ectoderm Figure 2.4 Teratomas were extracted from SCID...
- 117
- 385
- 0
Interim Method for Determinationof Melamine and Cyanuric AcidResidues In Foods using LC-MS/MS:Version 1.0
Ngày tải lên :
06/10/2016, 09:29
... all add 250 ng (250 µl of µg/ml stock) of isotopically labeled Melamine C To all add 2500 ng (250 µl of 10 µg/ml stock) of isotopically labled Cyanuric Acid D To the spikes, add desired amount ... mL from clear or supernatant layer if possible) Open valves, allow to gravity drip Add about mL acetonitrile and allow to gravity drip Add about mL of 0.20% DEA in acetonitrile and allow to gravity ... Isotopically Labeled Cyanuric Acid: Isotec; 13C3 Cyanuric Acid Prepare isotopically labeled Cyanuric Acid stock solution at µg/mL solution in acetonitrile Native Cyanuric Acid (TCI #C0459) a Stock...
- 27
- 409
- 0
Xác định hóa chất bảo vệ thực vật carbamat trong một số loại rau quả bằng phương pháp sắc ký lỏng khối phổ (LC-MS)
Ngày tải lên :
31/10/2012, 08:48
... detector ECD phòng sử dụng detector MS Giới hạn phát phương pháp thấp, khoảng 5µg/kg detector ECD 0,5µg/kg detector MS.[14] Xác định thuốc trừ sâu carbamat nhóm nito hữu thực phẩm phương pháp sắc ... gradient nồng độ 2.2.2.4 Detector HPLC Detector phận quan trọng định độ nhạy phương pháp Tuỳ thuộc chất lí hoá chất phân tích mà lựa chọn detector cho phù hợp Detector quang phổ hấp thụ phân tử ... trừ sâu số thực phẩm táo, khoai tây, gạo, chuối… Mẫu chiết aceton làm loại cột chiết pha rắn khác nhau: cột diatomaceous, cột diatomit kết hợp với cột C18, cột florisil Hiệu suất chiết mẫu thực...
- 85
- 3.2K
- 23
Xác định hóa chất bảo vệ thực vật carbamat trong một số loại rau quả bằng phương pháp sắc ký lỏng khối phổ (LC-MS)
Ngày tải lên :
18/03/2013, 11:04
... detector ECD phòng sử dụng detector MS Giới hạn phát phương pháp thấp, khoảng 5µg/kg detector ECD 0,5µg/kg detector MS.[14] Xác định thuốc trừ sâu carbamat nhóm nito hữu thực phẩm phương pháp sắc ... gradient nồng độ 2.2.2.4 Detector HPLC Detector phận quan trọng định độ nhạy phương pháp Tuỳ thuộc chất lí hoá chất phân tích mà lựa chọn detector cho phù hợp Detector quang phổ hấp thụ phân tử ... trừ sâu số thực phẩm táo, khoai tây, gạo, chuối… Mẫu chiết aceton làm loại cột chiết pha rắn khác nhau: cột diatomaceous, cột diatomit kết hợp với cột C18, cột florisil Hiệu suất chiết mẫu thực...
- 84
- 1.2K
- 7
NGHIÊN CỨU ĐỊNH LƯỢNG ĐỘC TỐ SINH HỌC BIỂN ASP TRONG THỦY SẢN VÀ SẢN PHẨM THỦY SẢN BẰNG PHƯƠNG PHÁP SẮC KÝ LỎNG GHÉP KHỐI PHỔ TANDEM LC-MS/MS
Ngày tải lên :
16/04/2013, 19:56
... Array Detector European Formic acid Fluorescence Detector Tiếng Việt Axít Domoic Đầu dò Diot Array Liên minh Châu Âu Axít Formic Đầu dò huỳnh quang HPLC High Performance Liquid Chromatography Liquid ... Chromatography Liquid Chromatograph Tandem Mass Spectrometer Limit of Detection Limit of Quantitative Normal Phase Liquid Chromatography Not detected Reversed Phase Liquid Chromatography Solid Phase ... lý an chất lượng vệ sinh to n thực phẩm nước xuất EU tương đương (ii) Hệ thống phòng kiểm nghiệm tham gia vào công tác kiểm tra, chứng nhận chất lượng đảm bảo vệ sinh an to n thực phẩm nước xuất...
- 104
- 1.4K
- 4
Xác định hóa chất bảo vệ thực vật carbamat trong một số loại rau quả bằng phương pháp sắc ký lỏng khối phổ (LC MS) (2)
Ngày tải lên :
04/08/2013, 16:24
... detector ECD phòng sử dụng detector MS Giới hạn phát phương pháp thấp, khoảng 5µg/kg detector ECD 0,5µg/kg detector MS.[14] Xác định thuốc trừ sâu carbamat nhóm nito hữu thực phẩm phương pháp sắc ... gradient nồng độ 2.2.2.4 Detector HPLC Detector phận quan trọng định độ nhạy phương pháp Tuỳ thuộc chất lí hoá chất phân tích mà lựa chọn detector cho phù hợp Detector quang phổ hấp thụ phân tử ... trừ sâu số thực phẩm táo, khoai tây, gạo, chuối… Mẫu chiết aceton làm loại cột chiết pha rắn khác nhau: cột diatomaceous, cột diatomit kết hợp với cột C18, cột florisil Hiệu suất chiết mẫu thực...
- 41
- 727
- 1
Tài liệu Make-up Solvent Considerations for Prep and Semi-Prep LC/MS Mass-Based .... doc
Ngày tải lên :
24/01/2014, 04:20
... clamp) Outlet Column Inlet NO COMM 215 Liquid Handler NC UV/VIS Detector Outlet 819 Injection Module Inlet to waste to waste low flow to high flow mass spectro collect Flow Splitter Inlet Make-up Pump ... form the base peak of the spectrum (Acetonitrile adduct MW + 42 from (MH+Acet)+) The absence of Na+ may be due either to the absence of this metal ion or to the blockage by this solvent MNa + ... tend to add to the adduct problem via an intrinsic solvent adduct to the degree that acetonitrile-based systems IPA does form an adduct with many of the same compounds affected by acetonitrile...
- 14
- 495
- 0
Xác định đồng thời dư lượng kháng sinh nhóm nitrofuran trong một số loại thực phẩm tươi sống trên địa bàn hà nội bằng phương pháp sắc ký lỏng khối phổ LC MS MS
Ngày tải lên :
10/02/2014, 20:55
... 10 mg/l (Dr.EhrenstorferGmbH), độ tinh khiết 99% - AMOZ 10 mg/l (Dr.EhrenstorferGmbH), độ tinh khiết 99% - AHD.HCl (Dr.EhrenstorferGmbH), độ tinh khiết 99% - SEM.HCl (Dr.EhrenstorferGmbH), độ tinh ... performance liquid chromatography and liquid chromatography mass spectrometry Analyst, Vol.121, 14631468 19 E Verdon, P Couedor, P Sanders (2007), Multi-residue monitoring for the simultaneous ... nitrofurazone, nitrofurantoine, nifursol) in poultry muscle tissue through the detection of their five major metabolites (AOZ, AMOZ, SEM, AHD, DNSAH) by liquid chromatography coupled to electrospray tandem...
- 16
- 979
- 0
Tài liệu Lipases and Phospholipases in Drug Development pptx
Ngày tải lên :
17/02/2014, 05:20
... authorship, we have attempted to cover major aspects of lipase research, from genomics to drug discovery via validation of targets, structural biology, rational drug design, and drug lipase interaction ... available to an investigator in the field of lipase purification Lipases tend to follow very specific patterns of purification The techniques used to purify the lipase need to be optimized to obtain ... protein to a volatile buffer This can sometimes be done by ion-exchange chromatography, but other forms of chromatography can, rarely, this If the protein solution is to be frozen, stored as...
- 357
- 1.3K
- 1
Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
Ngày tải lên :
07/03/2014, 05:20
... has the obvious potential to increase apoptotic effects, due to the proximity of the ROS source to the target DNA, where the possibility of detoxification of the ROS prior to damage is reduced Additionally, ... statistically significant compared to those levels in the vector control cell line (P < 0.02) As expected, cells overexpressing PAOh4 exhibited a polyamine pool profile similar to control cells containing ... cells relative to vector control cells (P < 0.05) Although spermidine levels appear to be elevated in the cell lines overexpressing active SMO isoforms, these changes were not found to be statistically...
- 12
- 395
- 0