for rna and protein extraction

... GSK3b protein expression and phosphorylation in cells electroporated in the presence of no siRNA (Lanes and 2), CKAP4 siRNA (Lanes and 4), or scrambled non-target (NT) siRNA (Lanes and 6), and ... analysis of p53 protein expression in cells electroporated in the presence of no siRNA (Lanes and 2), CKAP4 siRNA (Lanes and 4), or scrambled non-target (NT) siRNA (Lanes and 6), and treated with ... siRNA (Lanes and 2), CKAP4 siRNA (Lanes and 4), or scrambled non-target (NT) siRNA (Lanes and 6), and treated with as-APF (APF) or its inactive control peptide (Pep) b-actin served as a standard...

Ngày tải lên: 10/08/2014, 10:20

... template for protein synthesis, and this is done through the process of RNA interference Double stranded RNA (dsRNA) known as small interfering RNA (siRNA) was found to bind to their complementary mRNA ... probe and target oligos for Union, Mahidol, Canton and A+ SNP variants (the mutation is highlighted in grey) Table 4.1 Sequence of miRNA (let7a, f and g), and probes used to detect for let7a and ... AuNP probes and the assembly process, AuNPs could be combined with various platforms (gel, TEM, DLS, fluorescence spectrometry) to achieve distinct and unique readouts for gene, RNA and protein biomarkers...

Ngày tải lên: 09/09/2015, 11:16

... its particular protein?  As in, people protein vs tree protein?  The answer is DNA! The species-particular DNA sequences produce the species-particular proteins  GENES code for proteins  GENES ... a sugar, and a nitrogen base  DNA is in a double strand  The nitrogen bases have compliment partners  Adenine-Thymine  Cytosine-Guanine Just a note about RNARNA is single-stranded and acts ... ribosomes  DNA and protein synthesis, then, happens this way:  DNA sequence codes (how letters are put together) produce messenger RNA sequence codes  The messenger RNA leaves the nucleus and attaches...

Ngày tải lên: 13/03/2014, 16:36

... it has been shown to be the best-performing statisticsonly measure for CE (cf Evert and Krenn (2001) and Krenn and Evert (2001)) and also for ATR (see Wermter and Hahn (2005)) Concerning more recent ... another widely used measure for multi-word ATR Recently, more linguistically informed algorithms have been introduced both for CE (Wermter and Hahn, 2004) and for ATR (Wermter and Hahn, 2005), which ... employed in the literature for grading the termhood / collocativity of collected candidates Among the most widespread ones, both for ATR and CE, are statistical and information-theoretic measures,...

Ngày tải lên: 31/03/2014, 01:20

... proteomic research because of its high sensitivity for proteins in low molecular weight range and the capability for high throughput screening, even for proteins with extreme characteristics (highly hydrophobic, ... without anticoagulation and allowed to clot at room temperature for up to hr; the samples were then centrifuged at 4℃ for at 10000 rpm The sera were frozen and stored at -80℃ for future analysis ... mixture was aspirated and spotted onto the gold-coated ProteinChip array After air-drying for minutes at room temperature, protein crystals on the chip were scanned with the ProteinChip (Model PBS...

Ngày tải lên: 25/10/2012, 11:18

... (lane 4) and RGG3 (lane 5) EMSA was performed with these proteins and 32P-labeled Htelo (C) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled Htelo (lanes and 2) or mut Htelo (lanes and 4) ... Fig Protein nucleic acid complexes (A) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled Htelo (lanes and 2) or rHtelo (lanes and 4) (B) EMSA was performed with RGG3 (lanes and 4) and ... sarcoma protein Fig Affinity of EWS for binding to G-quadruplex DNA (A) EMSA was performed with EWS (lanes and 4) and 32P-labeled ETS-1 (lanes and 4) or ssDNA L (lanes and 2) (B) EMSA was performed...

Ngày tải lên: 15/02/2014, 01:20

... expands the boundaries of signalling mediators, and it is therefore not unlikely that Sky and Mer could be involved in Table Affinities of Gas6 and protein S for Axl, Sky and Mer RTKs within and ... production of protein S and other coagulation factors [41] Before identification of its receptor, human protein S was shown to be a potent mitogen for human vascular smooth muscle cells, and a protein ... function FEBS Journal 273 (2006) 5231–5244 ª 2006 The Authors Journal compilation ª 2006 FEBS S Hafizi and B Dahlback ¨ Function of protein S as ligand for Axl RTKs Surprisingly, only a handful of studies...

Ngày tải lên: 19/02/2014, 05:20

... not hold for bigrams6 , whose probability threshold and mixture weights need to be best tuned on the task at hand Hypernym Extraction For hypernym extraction, we tested WCL-1, WCL-3 and Hearst’s ... in any domain and style, and needs no parameter tuning; The high performance as compared with the best-known methods for both definition and hypernym extraction Our approach outperforms the other ... , |sk |} and b ∈ {1, , |sj |}, Sa,b is a score of the matching between the a-th token of sk and the b-th token of sj , and M0,0 , M0,b and Ma,0 are initially set to for all a and b The matching...

Ngày tải lên: 20/02/2014, 04:20

... user can explore in an uniform way both new information nuggets and validated background information nuggets interactively Fig summarizes the main components and the information flow Figure 1: Blueprint ... representation and accuracy in the Google style Especially for the general themes the presentation of web snippets is more convenient and more easy to understand However when it comes to interesting and ... on such a node expands it, as shown in Fig A single touch on a node that cannot be expanded adds its label to the initial user query and triggers a new search with that expanded query Figure...

Ngày tải lên: 20/02/2014, 05:20

... (b) *(c), (c) -*(d), (d) *(e), and so on S t e p and Step for P r o c e d u r e are implemented in each state for (a), (b), (c), (d), (e), and so on In state (a) for Fig 4, the index memory's ... corresponds to the candidate level Candidates on the same level form one stream For example, in Fig 6(a), the character at the 3rd position has three candidates: the 1st candidate is ' ~ ' , ... Text Extraction Process for text parsing systems This process is necessary for natural language parsing, because it is the first step in the parsing However, it is more laborious for Japanese and...

Ngày tải lên: 21/02/2014, 20:20

... C-terminal extension remains unstructured and solvent exposed, and therefore able to perform its solubilizing role in the sHsp and in the complexes formed with target proteins during chaperone action ... has also been observed recently for wildtype and mutant forms of aB-crystallin [60,61] The E190A and E204A mutants performed poorly compared with the wild-type protein in both assays, most notably ... Structure and properties of the small heat shock proteins (sHsp) and their interaction with cytoskeleton proteins Biochemistry (Mosc) 67, 511–519 Macario AJL (1995) Heat-shock proteins and molecular...

Ngày tải lên: 07/03/2014, 04:20

... reporter and an endogenous gene Moreover, this vector also provides a robust marker for the artificial miRNA-transfected cells Results and Discussion Generation of miRNA-based RNAi vectors based on RNA ... some natural miRNAs C D Fig Strategy for improved RNAi knockdown and ⁄ or marker gene expression (A,B) In the original miRNA-based artificial miRNA expression vectors, pmiR155 (A) and pmiR30 (B), ... conserved sequences for mRNA splicing (5¢ donor, branch, and 3¢ acceptor sites) suffice for the efficient processing of pri-miRNAs In previous studies, the ORFs for marker proteins were placed...

Ngày tải lên: 07/03/2014, 11:20

... scrambling and easier sample handling compared to in vivo protein production render cell-free protein synthesis particularly attractive for high-throughput production of proteins and selective ... residue type information, the protein ligand interaction can be probed by [15N]-HSQC FEBS Journal 273 (2006) 4154–4159 ª 2006 The Authors Journal compilation ª 2006 FEBS 4157 15 N-labelled proteins ... general, proteins that can be produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for...

Ngày tải lên: 07/03/2014, 12:20

... 3413–3423 ª 2005 FEBS N Poulsen and N Kroger ¨ cells synthesize AMT and NAT protein or if translation of AMT and NAT mRNAs is inhibited, as we have demonstrated for gfp mRNA Exploiting the ability ... Therefore, the promoter of a C fusiformis fcp gene has been isolated and tested for its applicability in C fusiformis transformations Primers were designed based on a C fusiformis fcp cDNA sequence (cffcpA-3) ... transformation experiments) Thus, C fusiformis transformation using the new plasmid pCfcp-ble was 3–4 times more efficient than the previous method Cloning of the NR gene from C fusiformis and mRNA...

Ngày tải lên: 07/03/2014, 21:20

... °C for days Complex formation was detectable with 0.8 nM of GST-HuD protein (B) Gel retardation assay was performed with the 52 nt sense CU-rich RNA and GST-HuR; a RNA: protein complex was formed ... radioactive RNA for proteins and consequently a decrease in detection of proteins crosslinked Ó FEBS 2003 Hu-proteins control MARCKS mRNA stability (Eur J Biochem 270) 357 with the radioactive CU-rich RNA ... 3¢-UTR RNA by in vitro transcription and incubated the resulting transcript with cytoplasmic extract of quiescent Swiss 3T3 cells After RNaseT1 digestion and native PAGE the formation of two RNA: protein...

Ngày tải lên: 08/03/2014, 08:20

... p38 mitogen-activated protein kinase and c-Jun NH2-terminal kinase by double-stranded RNA and encephalomyocarditis virus: involvement of RNase L, protein kinase R., and alternative pathways Mol ... cloning and characterization of the human double-stranded RNA activated protein kinase induced by interferon Cell 62, 379–390 Hovanessian, A.G (1989) The double stranded RNA- activated protein ... A.E (1997) Physical association between STAT1 and the interferon-inducible protein kinase PKR and implications for interferon and double-stranded RNA signaling pathways EMBO J 16, 1291– 1304 ...

Ngày tải lên: 08/03/2014, 09:20

... precision scores for each tool, with the goal to highlight strengths and weaknesses and to compare them (Rizzo and Troncy, 2011b) The comparison aggregates all the evaluations performed and, finally, ... schema types: ontology (for DBpedia Spotlight and Zemanta), lightweight taxonomy (for AlchemyAPI, Evri and Wikimeta) or simple flat type lists (for Extractiv, OpenCalais and Wikimeta) A concept ... top of various NLP tools Its architecture follows the REST principles and provides a web HTML access for humans and an API for computers to exchange content in JSON or XML Both interfaces are...

Ngày tải lên: 08/03/2014, 21:20

... the epitope 100 for RCR-2 on sEPCR is on the face of sEPCR opposite to its known binding site for protein C The stability of immobilized RCR-2 as a capture ligand for sEPCR and protein C is illustrated ... protein C SDS ⁄ PAGE and Western blotting SDS ⁄ PAGE and Western blotting were performed using standard techniques Briefly, for Western blot analysis, protein C or sEPCR were loaded and separated by ... K (1996) The Gla26 residue of protein C is required for the binding of protein C to thrombomodulin and endothelial cell protein C receptor, but not to protein S and factor Va Thromb Haemost 75,...

Ngày tải lên: 16/03/2014, 18:20

... licence and is freely available for download after completing a registration form4 Conclusions and Related Information This demonstration paper describes the ACCURAT toolkit containing tools for ... workflow of the toolkit allows NE and terminology extraction and mapping Starting with named entity recognition, the toolkit features the first NER systems for Latvian and Lithuanian (Pinnis, 2012) ... equivalents table and (ii) string similarity in terms of Levenshtein distance between term candidates For evaluation, Eurovoc (Steinberger et al., 2002) was used Tables and show the performance figures...

Ngày tải lên: 16/03/2014, 20:20