for rna and protein extraction
báo cáo khoa học: "Antiproliferative factor decreases Akt phosphorylation and alters gene expression via CKAP4 in T24 bladder carcinoma cells" docx
... GSK3b protein expression and phosphorylation in cells electroporated in the presence of no siRNA (Lanes and 2), CKAP4 siRNA (Lanes and 4), or scrambled non-target (NT) siRNA (Lanes and 6), and ... analysis of p53 protein expression in cells electroporated in the presence of no siRNA (Lanes and 2), CKAP4 siRNA (Lanes and 4), or scrambled non-target (NT) siRNA (Lanes and 6), and treated with ... siRNA (Lanes and 2), CKAP4 siRNA (Lanes and 4), or scrambled non-target (NT) siRNA (Lanes and 6), and treated with as-APF (APF) or its inactive control peptide (Pep) b-actin served as a standard...
- 11
- 256
- 0
Development of gold nanoparticle DNA nanostructure assembly for detection of DNA, RNA and protein biomarkers
... template for protein synthesis, and this is done through the process of RNA interference Double stranded RNA (dsRNA) known as small interfering RNA (siRNA) was found to bind to their complementary mRNA ... probe and target oligos for Union, Mahidol, Canton and A+ SNP variants (the mutation is highlighted in grey) Table 4.1 Sequence of miRNA (let7a, f and g), and probes used to detect for let7a and ... AuNP probes and the assembly process, AuNPs could be combined with various platforms (gel, TEM, DLS, fluorescence spectrometry) to achieve distinct and unique readouts for gene, RNA and protein biomarkers...
- 142
- 369
- 0
Lecture 3 DNA RNA and protein synthesis great
... its particular protein? As in, people protein vs tree protein? The answer is DNA! The species-particular DNA sequences produce the species-particular proteins GENES code for proteins GENES ... a sugar, and a nitrogen base DNA is in a double strand The nitrogen bases have compliment partners Adenine-Thymine Cytosine-Guanine Just a note about RNARNA is single-stranded and acts ... ribosomes DNA and protein synthesis, then, happens this way: DNA sequence codes (how letters are put together) produce messenger RNA sequence codes The messenger RNA leaves the nucleus and attaches...
- 17
- 671
- 2
Báo cáo khoa học: "You Can’t Beat Frequency (Unless You Use Linguistic Knowledge) – A Qualitative Evaluation of Association Measures for Collocation and Term Extraction" pot
... it has been shown to be the best-performing statisticsonly measure for CE (cf Evert and Krenn (2001) and Krenn and Evert (2001)) and also for ATR (see Wermter and Hahn (2005)) Concerning more recent ... another widely used measure for multi-word ATR Recently, more linguistically informed algorithms have been introduced both for CE (Wermter and Hahn, 2004) and for ATR (Wermter and Hahn, 2005), which ... employed in the literature for grading the termhood / collocativity of collected candidates Among the most widespread ones, both for ATR and CE, are statistical and information-theoretic measures,...
- 8
- 435
- 0
Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... proteomic research because of its high sensitivity for proteins in low molecular weight range and the capability for high throughput screening, even for proteins with extreme characteristics (highly hydrophobic, ... without anticoagulation and allowed to clot at room temperature for up to hr; the samples were then centrifuged at 4℃ for at 10000 rpm The sera were frozen and stored at -80℃ for future analysis ... mixture was aspirated and spotted onto the gold-coated ProteinChip array After air-drying for minutes at room temperature, protein crystals on the chip were scanned with the ProteinChip (Model PBS...
- 9
- 530
- 1
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... (lane 4) and RGG3 (lane 5) EMSA was performed with these proteins and 32P-labeled Htelo (C) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled Htelo (lanes and 2) or mut Htelo (lanes and 4) ... Fig Protein nucleic acid complexes (A) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled Htelo (lanes and 2) or rHtelo (lanes and 4) (B) EMSA was performed with RGG3 (lanes and 4) and ... sarcoma protein Fig Affinity of EWS for binding to G-quadruplex DNA (A) EMSA was performed with EWS (lanes and 4) and 32P-labeled ETS-1 (lanes and 4) or ssDNA L (lanes and 2) (B) EMSA was performed...
- 11
- 786
- 0
Tài liệu Báo cáo khoa học: Gas6 and protein S Vitamin K-dependent ligands for the Axl receptor tyrosine kinase subfamily pptx
... expands the boundaries of signalling mediators, and it is therefore not unlikely that Sky and Mer could be involved in Table Affinities of Gas6 and protein S for Axl, Sky and Mer RTKs within and ... production of protein S and other coagulation factors [41] Before identification of its receptor, human protein S was shown to be a potent mitogen for human vascular smooth muscle cells, and a protein ... function FEBS Journal 273 (2006) 5231–5244 ª 2006 The Authors Journal compilation ª 2006 FEBS S Hafizi and B Dahlback ¨ Function of protein S as ligand for Axl RTKs Surprisingly, only a handful of studies...
- 14
- 600
- 0
Tài liệu Báo cáo khoa học: "Learning Word-Class Lattices for Definition and Hypernym Extraction" doc
... not hold for bigrams6 , whose probability threshold and mixture weights need to be best tuned on the task at hand Hypernym Extraction For hypernym extraction, we tested WCL-1, WCL-3 and Hearst’s ... in any domain and style, and needs no parameter tuning; The high performance as compared with the best-known methods for both definition and hypernym extraction Our approach outperforms the other ... , |sk |} and b ∈ {1, , |sj |}, Sa,b is a score of the matching between the a-th token of sk and the b-th token of sj , and M0,0 , M0,b and Ma,0 are initially set to for all a and b The matching...
- 10
- 567
- 0
Tài liệu Báo cáo khoa học: "A Mobile Touchable Application for Online Topic Graph Extraction and Exploration of Web Content" ppt
... user can explore in an uniform way both new information nuggets and validated background information nuggets interactively Fig summarizes the main components and the information flow Figure 1: Blueprint ... representation and accuracy in the Google style Especially for the general themes the presentation of web snippets is more convenient and more easy to understand However when it comes to interesting and ... on such a node expands it, as shown in Fig A single touch on a node that cannot be expanded adds its label to the initial user query and triggers a new search with that expanded query Figure...
- 6
- 458
- 0
Tài liệu Báo cáo khoa học: "A HARDWARE ALGORITHM FOR HIGH SPEED MORPHEME EXTRACTION AND ITS IMPLEMENTATION" pptx
... (b) *(c), (c) -*(d), (d) *(e), and so on S t e p and Step for P r o c e d u r e are implemented in each state for (a), (b), (c), (d), (e), and so on In state (a) for Fig 4, the index memory's ... corresponds to the candidate level Candidates on the same level form one stream For example, in Fig 6(a), the character at the 3rd position has three candidates: the 1st candidate is ' ~ ' , ... Text Extraction Process for text parsing systems This process is necessary for natural language parsing, because it is the first step in the parsing However, it is more laborious for Japanese and...
- 8
- 504
- 0
Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... C-terminal extension remains unstructured and solvent exposed, and therefore able to perform its solubilizing role in the sHsp and in the complexes formed with target proteins during chaperone action ... has also been observed recently for wildtype and mutant forms of aB-crystallin [60,61] The E190A and E204A mutants performed poorly compared with the wild-type protein in both assays, most notably ... Structure and properties of the small heat shock proteins (sHsp) and their interaction with cytoskeleton proteins Biochemistry (Mosc) 67, 511–519 Macario AJL (1995) Heat-shock proteins and molecular...
- 14
- 417
- 0
Báo cáo khoa học: Design of expression vectors for RNA interference based on miRNAs and RNA splicing potx
... reporter and an endogenous gene Moreover, this vector also provides a robust marker for the artificial miRNA-transfected cells Results and Discussion Generation of miRNA-based RNAi vectors based on RNA ... some natural miRNAs C D Fig Strategy for improved RNAi knockdown and ⁄ or marker gene expression (A,B) In the original miRNA-based artificial miRNA expression vectors, pmiR155 (A) and pmiR30 (B), ... conserved sequences for mRNA splicing (5¢ donor, branch, and 3¢ acceptor sites) suffice for the efficient processing of pri-miRNAs In previous studies, the ORFs for marker proteins were placed...
- 7
- 514
- 0
Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... scrambling and easier sample handling compared to in vivo protein production render cell-free protein synthesis particularly attractive for high-throughput production of proteins and selective ... residue type information, the protein ligand interaction can be probed by [15N]-HSQC FEBS Journal 273 (2006) 4154–4159 ª 2006 The Authors Journal compilation ª 2006 FEBS 4157 15 N-labelled proteins ... general, proteins that can be produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for...
- 6
- 461
- 0
Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx
... 3413–3423 ª 2005 FEBS N Poulsen and N Kroger ¨ cells synthesize AMT and NAT protein or if translation of AMT and NAT mRNAs is inhibited, as we have demonstrated for gfp mRNA Exploiting the ability ... Therefore, the promoter of a C fusiformis fcp gene has been isolated and tested for its applicability in C fusiformis transformations Primers were designed based on a C fusiformis fcp cDNA sequence (cffcpA-3) ... transformation experiments) Thus, C fusiformis transformation using the new plasmid pCfcp-ble was 3–4 times more efficient than the previous method Cloning of the NR gene from C fusiformis and mRNA...
- 11
- 668
- 0
Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt
... °C for days Complex formation was detectable with 0.8 nM of GST-HuD protein (B) Gel retardation assay was performed with the 52 nt sense CU-rich RNA and GST-HuR; a RNA: protein complex was formed ... radioactive RNA for proteins and consequently a decrease in detection of proteins crosslinked Ó FEBS 2003 Hu-proteins control MARCKS mRNA stability (Eur J Biochem 270) 357 with the radioactive CU-rich RNA ... 3¢-UTR RNA by in vitro transcription and incubated the resulting transcript with cytoplasmic extract of quiescent Swiss 3T3 cells After RNaseT1 digestion and native PAGE the formation of two RNA: protein...
- 16
- 754
- 0
Báo cáo Y học: Double-stranded RNA-activated protein kinase interacts with apoptosis signal-regulating kinase 1 Implications for apoptosis signaling pathways docx
... p38 mitogen-activated protein kinase and c-Jun NH2-terminal kinase by double-stranded RNA and encephalomyocarditis virus: involvement of RNase L, protein kinase R., and alternative pathways Mol ... cloning and characterization of the human double-stranded RNA activated protein kinase induced by interferon Cell 62, 379–390 Hovanessian, A.G (1989) The double stranded RNA- activated protein ... A.E (1997) Physical association between STAT1 and the interferon-inducible protein kinase PKR and implications for interferon and double-stranded RNA signaling pathways EMBO J 16, 1291– 1304 ...
- 7
- 361
- 0
Báo cáo khoa học: "A Framework for Unifying Named Entity Recognition and Disambiguation Extraction Tools" pot
... precision scores for each tool, with the goal to highlight strengths and weaknesses and to compare them (Rizzo and Troncy, 2011b) The comparison aggregates all the evaluations performed and, finally, ... schema types: ontology (for DBpedia Spotlight and Zemanta), lightweight taxonomy (for AlchemyAPI, Evri and Wikimeta) or simple flat type lists (for Extractiv, OpenCalais and Wikimeta) A concept ... top of various NLP tools Its architecture follows the REST principles and provides a web HTML access for humans and an API for computers to exchange content in JSON or XML Both interfaces are...
- 4
- 466
- 0
Báo cáo khoa học: Selective modulation of protein C affinity for EPCR and phospholipids by Gla domain mutation pdf
... the epitope 100 for RCR-2 on sEPCR is on the face of sEPCR opposite to its known binding site for protein C The stability of immobilized RCR-2 as a capture ligand for sEPCR and protein C is illustrated ... protein C SDS ⁄ PAGE and Western blotting SDS ⁄ PAGE and Western blotting were performed using standard techniques Briefly, for Western blot analysis, protein C or sEPCR were loaded and separated by ... K (1996) The Gla26 residue of protein C is required for the binding of protein C to thrombomodulin and endothelial cell protein C receptor, but not to protein S and factor Va Thromb Haemost 75,...
- 12
- 409
- 0
Báo cáo khoa học: "Toolkit for Multi-Level Alignment and Information Extraction from Comparable Corpora" pptx
... licence and is freely available for download after completing a registration form4 Conclusions and Related Information This demonstration paper describes the ACCURAT toolkit containing tools for ... workflow of the toolkit allows NE and terminology extraction and mapping Starting with named entity recognition, the toolkit features the first NER systems for Latvian and Lithuanian (Pinnis, 2012) ... equivalents table and (ii) string similarity in terms of Levenshtein distance between term candidates For evaluation, Eurovoc (Steinberger et al., 2002) was used Tables and show the performance figures...
- 6
- 289
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008