for cytochrome cd1 nitrite reductase nire

... Structure of cytochrome c nitrite reductase. Nature 400, 476–480. 15. Einsle, O., Stach, P., Messerschmidt, O.A., Simon, J., Kro ¨ ger, A., Huber, R. & Kroneck, P.M.H. (2000) Cytochrome c nitrite reductase ... dithiothreitol, boiling for 1 min. The gel was stained for heme c.(C)Gelof16· 18 cm. Protein treatment: no dithiothreitol, no boiling. The gel was stained for nitrite reductase activity. 3906 ... properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. J. Biol. Chem. 271, 23191–23196. 25. Liu, M.C., Costa, C. & Moura, I. (1994) Hexaheme nitrite reductase...

Ngày tải lên: 21/02/2014, 00:20

... Methylomonas sp.; cd 1 -Nir, cytochrome cd 1 nitrite reductase; Cu-Nir, copper-containing nitrite reductase isolated from Pseudomonas chlororaphis DSM 50135; cyt., cytochrome; DDC, diethyldithiocarbamate; ... of the as-purified (blue) form of the Ps. chlororaphis DSM 50135 nitrite reductase. [Cu-Nir] ¼ 23 l M in 20 m M Tris/HCl buffer pH 7.6. Ó FEBS 2004 Cu-containing nitrite reductase from Ps. chlororaphis ... protein. The pI of other copper nitrite reductases described in the literature are also acidic, except for the Al. xylosoxidans NCIB 11015 protein [1]. The pI deter- mined for Ps. aureofaciens Cu-Nir...

Ngày tải lên: 07/03/2014, 15:20

... of the acidic region responsible for cytochrome c docking. While no direct structural information is at hand for the docked complex, the interaction domain for cytochrome c on the cyto- chrome ... the reaction. For an alternative explanation to account for this phenomenon, we find no evidence for a second cytochrome c binding site on oxidase. Keywords: Paracoccus denitrificans; cytochrome c ... R. & Bosshard, H.R. (1980) Comparison of the binding sites on cytochrome c for cytochrome c oxidase, cytochrome bc 1 and cytochrome c 1 . J. Biol. Chem. 255, 4732–4739. 8. Taha, T.S.M. &...

Ngày tải lên: 22/02/2014, 07:20

Ngày tải lên: 05/09/2013, 09:38

... vitro. Initially we performed enzyme assays with all three NirE proteins – NirE- His, Trx-S -NirE- His and S -NirE- His – and compared their catalytic activities. We observed that Trx-S -NirE- His and S -NirE- His ... vectors For the construction of nirE expression vectors, the nirE gene from P. aeruginosa PAO1 was PCR amplified using the primers nirE_ Pa_BamHI _for (GCCG GGATCCAT GAACACTACCGTGATTC) and nirE_ Pa_XhoI_rev (GA CTCGAGGGCGCATGCGAC) ... respectively, for cloning the nirE gene into pET32a (Novagen, Darmstadt, Germany). For cloning the nirE gene into pET22b (Nov- agen), the PCR primers NirE_ NdeI (GT CATATGACA CTACCGTGATTCC) and NirE_ HindIII...

Ngày tải lên: 18/02/2014, 06:20

... resulted in the formation of 20,23-di- hydroxyvitamin D3 (RT = 30 min) and trihydroxyvi- tamin D3 (RT = 22 min) (Fig. 4A). A small lag (0– 3 min) was seen in the time course for formation of trihydroxyvitamin ... by cytochrome P450scc R. C. Tuckey et al. 2596 FEBS Journal 275 (2008) 2585–2596 ª 2008 The Authors Journal compilation ª 2008 FEBS Pathways and products for the metabolism of vitamin D3 by cytochrome ... correlations for 3-CH and 23-CH; (B) expan- sion of proton–proton TOCSY correlations for 3-CH and 23-CH; (C) expansion of proton–carbon HSQC showing groups having correla- tion to 3-CH and 23-CH (for...

Ngày tải lên: 18/02/2014, 17:20

... for the model reaction of NADPH cytochrome P450 oxidoreductase with cytochrome c. Biochemistry 33, 12012–12021. S. Brenner et al. Electron transfer in cytochrome P450 reductase FEBS Journal 275 (2008) ... NADPH cytochrome P450 oxidore- ductase with cytochrome P450 and cytochrome c. J Biol Chem 270, 27475–27480. 27 Shen AL & Kasper CB (1996) Role of Ser457 of NADPH cytochrome P450 oxidoreductase ... model NADPH cytochrome P450 oxidore- ductase reaction with cytochrome c. Biochemistry 34, 12768–12774. 25 Shen AL, Christensen MJ & Kasper CB (1991) NADPH cytochrome P-450 oxidoreductase....

Ngày tải lên: 07/03/2014, 06:20

... model for nitrate reductase A from Escherichia coli. Keywords: cytochrome b; electron transfer; Escherichia coli; nitrate reductase A; quinone. Nitrate can be used as an electron acceptor for anaerobic growth ... order to clarify the role of cytochrome in nitrate reductase we have performed spectrophotometric and stopped-flow kinetic studies of reduction and oxidation of the cytochrome hemes with analogues ... interaction between the cytochrome and the quinones by studying inhibition of the nitrate reductase activity by HOQNO. It has therefore clarified the binding site of the menaquinols, but for technical reasons...

Ngày tải lên: 07/03/2014, 15:20

... few water molecules is formed upo n Fe 3 + –NO c omplex formation, and that the network should play a key role in providing a proton that i s required for intermediate formation [9,10]. As shown previously, ... concentrations for kinetic analysis when these mutant proteins are utilized, which is impossible with the wild-type P450nor, a s noted above. As shown in Fig. 5, the k obs for the reduction step for the ... trace of I formation can avoid the interf erence due to the s pontan eous dec omposition of I that follows its formation, although the rate of decomposition is m uch slower than that of I formation...

Ngày tải lên: 07/03/2014, 15:20

... was performed with whole-cell DNA as the template according to the following parameters: 94 °C for 4 min; 10 cycles of 94 °C for 15 s, 52 °C for 30 s, 72 °C for 30 s; 20 cycles of 94 °C for 15 ... Fe 3 S 4 ferredoxin. An absolute requirement for ferredoxin in cyto- chrome c reduction has been shown for several P450 reductases [32–34]. FdR mor was capable of reducing cytochrome c on its own, although ... results were obtained for flavodoxin reductase from E. coli [35] and ferredoxin reductase from Streptomyces griseus [36]. In contrast to the latter and to putidaredoxin reductase [32], the two-electron...

Ngày tải lên: 07/03/2014, 16:20

... scheme proposed for the S. aureus CoADR. It is interesting to note, however, that the overall shape of the titration curve at 452 nm for the sta- phylococcal enzyme and 460 nm for the phCoADR ... it makes sense for the enzyme to stabilize the EH 4 form, since the reduced flavin is reactive with O 2 . Other enzymes in the family, such as the NADH peroxidase or glutathione reductase, tend ... disul- fide reductase family can be divided into two classes: (a) enzymes whose function appears to be the main- tenance of a high R-SH ⁄ R-S-S-R ratio, such as glutathione reductase, trypanathione reductase...

Ngày tải lên: 07/03/2014, 17:20

... RNA products were used for primer extension experi- ments.Thesameprimer,specificfortheluciferaseopen-readingframe was used for all constructs. Arrows indicate the position for the two major transcription ... reporter gene in stably transformed cells, even at high concentrations of polyamide. We realized that we had taken for granted that the R2 TATA-box is essential for transcription initiation at ... extracts for 15 min on ice in the binding buffer before adding the labeled probe. Transfection of cells, serum starvation and luciferase assays Transient transfection, isolation of stably transformed...

Ngày tải lên: 08/03/2014, 02:20

... P450 is therefore indicative rather for a high solvent accessibility of the heme pocket than for a diffusion limited process. Subconformers of P450cam have different k on and D V # for CO binding The ... studied, we note that for the class I substrates CO binding is disfavoured because of a rigid heme pocket and the search for the optimal place near the heme for CO-iron bond formation appears to ... dithionite, values for concentrations correspond to the mixture. b The mean values for k on and DV # are given with their ± SD. Fig. 3. Plot of lnk on against the pressure for cytochrome P450cam bound...

Ngày tải lên: 08/03/2014, 23:20

... deuter- ium isotope effects for both 7-OMe and 7-OEt coumarin dealkylation reac- tions. The apparent intrinsic isotope effect for P450 1A2 (9.4 for O-demethylation, 6.1 for O-deethylation) showed ... (1984) Kinetic isotope effects on cytochrome P-450-catalyzed oxidation reactions: evidence for the irreversible forma- tion of an activated oxygen intermediate of cytochrome P-448. J Biol Chem 259, ... (9.6 for O-demethylation, 6.1 for O-deethylation) was attenu- ated in the noncompetitive intermolecular experiments. High noncompeti- tive intermolecular kinetic isotope effects were seen for...

Ngày tải lên: 16/03/2014, 13:20

... an extinction coefficient of 91 mm )1 Æcm )1 (A 450)490 ) for P450 and 111 mm )1 Æcm )1 (A 420)490 ) for the cytochrome P420 [23,24]. When both P450 forms were present, the total P450 content was estimated ... as a cytochrome P450. Science 25, 781–784. 36 Kochs G, Werck-Reichhart D & Grisebach H (1992) Further characterization of cytochrome P450 involved in phytoalexin synthesis in soybean: cytochrome ... the purification of plant cytochromes P-450 and b5. Anal Biochem 197, 125–131. 40 Andersen MD & Moller BL (1998) Double Triton X-114 phase purification for the plant cytochromes P450 and removal...

Ngày tải lên: 16/03/2014, 23:20

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Ngày tải lên: 22/06/2014, 06:20