following protein glycosaminoglycan polysaccharide interactions with differential scanning fluorimetry
Báo cáo khoa học: Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1 doc
... localize proteins to the plasma membrane, where they can interact with phosphoinositides and membrane proteins [21] The FERM domain of talin interacts with phosphatidylinositol 4,5-P2 (PIP2) and with ... full-length protein interact with Rap1, as shown by pull-down and asolectin vesicle sedimentation experiments Moreover, HypoKrit1 interacts with a higher affinity with Rap1GTPcS than with Rap1GDP, ... staining of the proteins with Sypro Orange As expected, Rap1GTPcS did not interact with GST-ICAP-1 Interestingly, when Krit1 and Rap1GTPcS were added together, Rap1GTPcS was pulled-down with Krit1...
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Báo cáo khoa học: Cdc37 maintains cellular viability in Schizosaccharomyces pombe independently of interactions with heat-shock protein 90 doc
... Cdc37 truncation protein with that of endogenous full length protein All truncation mutants except for Cdc37(1–155) yielded a truncated protein that was detected by western blot with the Cdc37 ... Disruption of the protein structure in this region might affect essential N-terminal interactions between Cdc37 and client proteins Whatever the reason, it appears that Cdc37 proteins with a defective ... essential role, independent of interactions with Hsp90 However, biochemical investigations reveal that a small proportion of total Cdc37 protein is associated with the cochaperone Hsp90 in a...
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Báo cáo Y học: Differential scanning calorimetric study of myosin subfragment 1 with tryptic cleavage at the N-terminal region of the heavy chain pdf
... F-actin were also examined in these experiments Differential scanning calorimetry (DSC) DSC experiments were performed on a DASM-4M differential scanning microcalorimeter (Institute for Biological ... ternary complexes of t-S1 and Nt-S1 with ADP-Vi and ADP-BeFx, in comparison with the proteins containing ADP alone (control curves shown by dashed lines) For both proteins, the formation of the ternary ... denaturation of all protein samples studied was fully irreversible This irreversibility can be explained by protein aggregation which was shown to occur after heating of S1 and its complexes with nucleotides...
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Báo cáo khoa học: Deciphering the key residues in Plasmodium falciparum b-ketoacyl acyl carrier protein reductase responsible for interactions with Plasmodium falciparum acyl carrier protein pptx
... suggests that the interactions of PfFabG with the 4¢-phosphopantetheine moiety of PfACP are hydrophobic in nature Plasmodium FabG with a lone tryptophan provides an ideal system with which to study ... by these residues in the interactions of PfFabG and PfACP, we generated the following mutants: R187E, R230E, R187A ⁄ R230A, R187E ⁄ R230E, R190E, Interactions of PfFabG with PfACP R190A and R230K ... interact, but not much is known about the residues involved in interactions Plasmodium falciparum ACP (PfACP) is a small protein, with a flexible conformation, which shuttles the substrates between...
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Báo cáo Y học: Expression of recombinant murine pregnancy-associated plasma protein-A (PAPP-A) and a novel variant (PAPP-Ai) with differential proteolytic activity pot
... incubated (24 h) with medium from mock-transfected cells (lane 1), with medium from cells transfected with murine PAPP-A cDNA (lanes 2–3), with murine PAPP-Ai cDNA (lanes 4–5), or with human PAPP-A ... radiolabeled binding protein All reactions were incubated at 37 °C (up to 72 h) as specified in the text Both of the binding proteins were expressed as C-terminally tagged proteins causing the ... metalloproteinases [19,20], a diverse group of zinc peptidases comprised of five families: the astacins (e.g bone morphogenetic protein- 1), the reprolysins or adamalysins (snake venom proteinases,...
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HCV functional genomic protein interactions with NS3 and their role in viral replication
... self-interaction in NS3 Protein- protein interactions among all HCV proteins were tested and NS3 was found to interact strongly with itself To delineate the region(s) of NS3 important for interaction with itself, ... temperature with gentle agitation The beads were washed four times with lysis buffer supplemented with mM PMSF, and the bound proteins separated on SDS-PAGE gels The gels were dried and bound proteins ... of these interactions Yeast-two hybrid screens were set up to identify HCV proteins as well as host proteins that interact with NS3, using a spleen cDNA library The functions of these interactions...
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POLYSACCHARIDE ESTERS WITH DEFINED FUNCTIONALISATION PATTERN
... (reproduced with permission from [446], copyright Wiley VCH) 180 Polysaccharide Esters with Defined Functionalisation Pattern Deprotection is carried out by treatment of the polysaccharide derivatives with ... behaviour, and especially in interactions with human blood [360] Cellulose acetate phosphates with defined functionalisation pattern have been prepared [428] The mixed esters with phosphate moieties ... phosphorylation with polyphosphoric acid in DMF in the presence of tributyl amine yields cellulose acetate phosphate with DSAcetyl 0.83 and DSP 1.20, which may be deacetylated by 172 Polysaccharide Esters with...
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Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... mediating interactions between tau and Fyn-SH3, and this further suggests that different, and possibly independent, mechanisms are involved in these interactions of tau with the same protein Interactions ... insoluble debris Following determination of protein concentration by bicinchoninic acid protein assay (Thermo Scientific, Rockford, IL, USA), equal amounts of protein were incubated with GST, GST– ... cotransfected with Fyn together with either wild-type or the YF mutant forms of tau Cell lysates containing equal amounts of tau were subjected to pulldown assays with GST–Fyn-SH3, and GST-bound proteins...
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Tài liệu The Acquisition of Drugs and Biologics for Chemical and Biological Warfare Defense - Department of Defense Interactions with the Food and Drug Administration doc
... point of contact within the pharmaceutical firm coordinates all interactions with FDA for a specific product What options does DoD have in addressing the management of its relations with FDA? Three ... development or acquisition of drugs and biologics for CBW defense and interactions with FDA Acquisition involves three major interactions between DoD and FDA related to CBW defense: (1) licensing ... context, DoD interactions with FDA are a critical and oftenoverlooked issue DoD responsibility for CBW drug and biologics development is distributed across several organizations within the department...
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Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt
... VCP–Cdc48p interacts with Ube4b–Ufd2p and with other VBM-containing proteins Evolutionary divergence in VCP binding during ERAD We hypothesized that additional ubiquitin-metabolizing proteins would ... VBM-containing proteins to VCP As discussed above, several proteins bind the N-domain of VCP in a mutually exclusive manner, including the VBM-containing proteins Atx-3 and Ube4b [13] Knowing which proteins ... compete with one another for binding is an essential step towards understanding how VCP interacts with other proteins inside a cell Thus, having identified Hrd1 as a new VBMcontaining protein...
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Tài liệu Báo cáo khoa học: Agrobacterium tumefaciens type II NADH dehydrogenase Characterization and interactions with bacterial and thylakoid membranes ppt
... expression of the protein Using an antibody raised against the recombinant protein, AtuNDH-2 was detected in protein extracts from the complemented ANN0222 strain (Fig 6C) Most of the protein was present ... concluded to interact with bacterial membranes and UQs and also with thylakoid membranes and PQs Although NDH-2s are membrane-bound enzymes, the nature of membrane ⁄ protein interactions has not ... against the purified AtuNDH-2 protein (Agro-Bio, Villeny, France) and further used to probe the presence of the AtuNDH-2 protein in soluble and membrane protein fractions Proteins were separated on...
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Tài liệu Báo cáo khoa học: A differential scanning calorimetry study of tetracycline repressor pptx
... differences in these values Differential scanning calorimetry DSC experiments were performed on a Calorimetry Sciences Corporation (CSC) 6100 Nano II differential scanning calorimeter with a cell volume ... concentration of the protein In the case of TetR, the Tmax obtained decreases with increasing protein concentration, which indicates that TetR unfolds without dissociation Indeed, if multimeric proteins ... regarding protein stability in vivo and its relation with misfolding diseases Proteins 40, 5870 42 Baszczyk, U & Wasylewski, Z (2003) Interaction of cAMP receptor protein from Escherichia coli with...
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Tài liệu Báo cáo khoa học: Protein farnesyltransferase inhibitors interfere with farnesyl diphosphate binding by rubber transferase pdf
... a-hydroxyfarnesylphosphonic acid (HFPA, 3) are known inhibitors of protein farnesyltransferases, which covalently modify proteins with a FPP molecule (Z)-2-octyl-3methylbutenedioic acid dilithium ... difference in length of the inhibitors allows to interact with the polyisoprene molecules within the rubber particle In both cases, the additional interactions result in tighter binding of when compared ... farnesyl -protein transferase blocks ras processing in vivo J Biol Chem 268, 7617–7620 Ratemi, E.S., Dolence, J.M., Poulter, C.D & Vederas, J.C (1996) Synthesis of protein farnesyltransferase and protein...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... a Waters 625 HPLC equipped with a Waters 490E dual wavelength detector was used Elution of the hydrolytic products and of the residual intact protein was performed with a linear gradient from ... by trypsin, whereas the concentration of the intact protein decreased with an accompanying increase in TP2 The pattern of events observed with chymotrypsin is made somewhat more complicated by ... facilitate access to otherwise buried structural regions of the protein may allow production of hydrolysates with improved qualities with respect to those presently on the market as for their sensory,...
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Applications of Calorimetry in a Wide Context - Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microcalorimetry docx
... Sunderland, United Kingdom Section Application of Differential Scanning Calorimetry into Pharmaceuticals Chapter Application of Differential Scanning Calorimetry to the Characterization of Biopolymers ... other side lignin with the important functional groups and the low molecular weight with the narrow polydispersity can be used as the stabilizer Application of Differential Scanning Calorimetry ... Calorimetry in a Wide Context - Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microcalorimetry” to reflect the content of the book Finally, without the support of many other...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... His6-tagged protein was puried from solubilized inclusion bodies using Ni(II)afnity chromatography under denaturing conditions As shown previously [3,4], the His6-tag does not interfere with protein ... different proteins (C) Fluorescence emission spectra (kex = 295 nm) of wild-type RepA (solid line), C160RepA both unlabeled (dashed line) and AEDANS labeled (dash-dots), recorded at 23.5 C with $ lM protein ... respectively, based on the crystal structure [7] of monomeric RepE in complex with iteron DNA a analogous decays with kem = 530 nm, with corresponding best ts and tabulated parameters, are supplied in...
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Báo cáo khoa học: A zinc finger HIT domain-containing protein, ZNHIT-1, interacts with orphan nuclear hormone receptor Rev-erbb and removes Rev-erbb-induced inhibition of apoCIII transcription potx
... full-length protein of 154 amino acids and shares high identity with its homologues (100% with Canis familiaris, 97% with Mus musculus, 87% with Xenopus tropicalis, 87% with Danio rerio, 68% with Drosophila ... transfected with plasmids pEGFP-Rev-erbb and pDsRed-ZNHIT-1, or cotransfected with both plasmids After 36 h of transfection, the cells were fixed with 4% formaldehyde and stained with lgÆmL)1 ... humans This protein contains a conserved zinc finger HIT domain originally found in the yeast HIT-1 protein [23], and so has been named ZNHIT-1 (‘zinc finger HIT domain-containing protein 1’) Previous...
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Báo cáo khoa học: Hepatocyte growth factor activator (HGFA): molecular structure and interactions with HGFA inhibitor-1 (HAI-1) doc
... KD1 (blue) with an atom within 4.0 A of the other protein (= binding region) are indicated KD1 makes contact with all substrate ⁄ inhibitor specificity-determining loops on HGFA (compare with Fig ... incompetent and competent conformations is probably quite low, well within the energy provided by substrate interactions, and consistent with the notion of ‘induced fit’ This is probably the case for ... HGFA protease domain with HAI-1-derived KD1 (PDB 1YC0) (A) KD1 (magenta) interacts with HGFA (beige) in a substrate-like manner by occupying subsites S4, S2 and S1 (in orange) with Arg258, Cys259–Cys283,...
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Effect of tunneling current on the growth of silicon islands on si(111) surfaces with a scanning tunneling microscope
... combined with a scanning reflection electron microscope (SREM ) The condition of the tungsten tip of the STM and the manipulation of the tip on the silicon surface could be monitored continuously with ... islands with the STM and examined the quality of STM images of the islands Note that after the creation of islands with the STM, the accumulation of silicon atoms on the tip apex was not observed with ... measured that the islands, grown with the STM, have a high aspect ratio (height divided by base length) of about 0.3 [7] We shall assume that all islands are cone-shaped with an aspect ratio of 0.3...
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Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx
... treats each protein as an unknown and evaluates its structure using the remaining proteins as a training set For the RaSP50 proteins, great care was taken to eliminate protein preparations with impurities ... better readability Proteins are sorted according to their a-helix content combinations were performed with the RaSP50 protein spectra The results presented in Table are given with the optimal normalization ... a useful validation method for protein spectra, at least within the proteins of the RaSP50 basis set The finding that the Mahalanobis distance does not correlate with analysis accuracy for the...
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