fluorescent liposomes and analysis of gene expression

... control of gene expression [16] Therefore, successful gene therapy of many genetic diseases may require stringent and appropriate control of vector-introduced gene expression The use of LCRs ... results in further enhancement of gene expression Insertion of intron of the ADA gene results in further enhancement of gene expression A SHGI7AL contains intron of the ADA gene in the inverted reverse ... low levels of gene expression often accompanied by high levels of clonal variation and the silencing of expression over time [40-42] In light of these difficulties, the successful use of lentiviral...

Ngày tải lên: 14/08/2014, 19:22

... replication in U1 and ACH-2 cells, and toxicity in uninfected JurPotencies Potencies of different HDACIs in terms of activation of HIV-1 replication in U1 and ACH-2 cells, and toxicity in uninfected ... only a small proportion of cells expressing GFP, and a correct estimate of the expression of this protein at subsequent time points was biased by the autofluorescence of dying cells The A1 clone, ... Jurkat cell clone (A1) fluorescent protein (GFP) by MS-275 and buthionine sulfoximine Stimulation of HIV-1 LTR-controlled expression of green fluorescent protein (GFP) by MS-275 and buthionine sulfoximine...

Ngày tải lên: 12/08/2014, 23:21

... decreased the expression of HO-2 mRNA and protein and induced the expression of HO-1 mRNA and protein In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins ... synthesis and heme degradation is responsible To explore the role of free heme in HO-1 and HO-2 expression, we treated HeLa and HepG2 cells with SA and determined the expression levels of HO-1 and ... analyzed the expression profiles of HO-1 and HO-2 in eight human cell lines by northern and western blot analyses (Fig 1A,B) Expression of HO-1 5334 A B C Fig Expression profiles of HO-1 and HO-2 in...

Ngày tải lên: 19/02/2014, 05:20

... difference shading’ [9] Our strategy is based on comparative analysis of lysates of cells both with and without transporter expression The expression of all other proteins in the two cell populations should ... a factor of 9.7) Thus, the background in the off-state was much lower with pEBTetD than with pEBTet However, in contrast to expression of membrane proteins, the level of expression of cytosolic ... al A B Fast set-up of inducible protein expression emphasis was therefore on the analysis of cell pools We not assume that our system is superior in terms of inducibility of single clones; moreover,...

Ngày tải lên: 23/03/2014, 09:21

... phosphorylation and therefore inactivation of the ACC1 isoform of acetyl CoA carboxylase (ACC) [12] or suppression of lipogenic gene expression, including ACC1 and fatty acid synthase, and increase of fatty ... of the experiments She received help from EvE, NA and DG on the performance of Western Blot analysis, from DG on the use of metformin, from DM, HS and CN for the care of melanoma cell lines and ... stimulating the growth of one cell line in our panel, and the antagonistic effects of the combination of metformin and vemurafenib in several cell lines, the combination of vemurafenib and metformin should...

Ngày tải lên: 18/06/2014, 19:20

... expression of these genes within humans and mice, of T-bet within Ginbuna crucian carp [27] and STAT6 in mandarin fish [28] have shown similar widespread expression In the case of STAT6, highest expression ... [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression ... h, and the total RNA was recovered Following reverse transcription, the relative expression of each gene was detected by real-time PCR and normalized to the expression of gapdh The means of six...

Ngày tải lên: 16/02/2014, 09:20

... was defined by both Triton X-100 and Chaps detergents, and found approximately independent of the ligand (IL-2) occupation level of receptors on T cells Analysis of the whole sucrose gradient sedimentation ... raft-association of the IL-2Rb chain in transformed human NK and fibroblast cells Thus, to better understand the role of lipid rafts in cell growth/ viability-signaling of T cells, in general, and in the ... methods indicated a significant degree of molecular vicinity between CD25 and class I HLA molecules on all cells, regardless of the expression level of b and cc chains or class I HLA (see MT-1...

Ngày tải lên: 17/03/2014, 23:20

... types of tumours as indicated in the figure and include carcinomas of different types (squamous and adenocarcinomas), sarcomas and T and B lymphomas (B) The mobility of endogenous VRK2A and VRK2B ... pairs downstream of exon 12 and 4542 base pairs upstream of exon 14, former exon 13, in the genomic sequence of the VRK2 gene (Fig 2A) The resulting VRK2 proteins, A and B, have 508 and 397 amino ... Differential stabilization of p53 by VRK2 isoforms Fig Expression of two VRK2 isoforms (A) Detection of VRK2 proteins in several cell lines that were determined by immunoblotting of whole-cell extracts...

Ngày tải lên: 30/03/2014, 11:20

... to make the most of the material in this book, you should possess a basic understanding of the PHP language, at least a conceptual understanding of object-oriented programming and preferably PHP's ... understanding of what solutions such as the Zend Framework have to offer Frameworks Support the Development of Dynamic Websites Dynamic websites, like any software application, are composed of three ... can be used to manage the placement and formatting of a wide variety of site assets and other data, including page titles, CSS and JavaScript files, images, and even URLs You can even create custom...

Ngày tải lên: 23/06/2014, 13:03

... regulator of gene expression The genetic basis of gene expression has been investigated across tissues [19] and populations [20] Both lines of evidence suggest genetic variants associated with gene expression ... not much is known about the precise mechanisms by which genetic variants modify gene- expression Combining genetic, epigenetic, and gene expression data can inform the underlying relationship between ... variation in both methylation and expression To better understand the role of genetic variation in controlling DNA methylation variation, and its resulting effects on gene expression variation, we...

Ngày tải lên: 09/08/2014, 22:23

... Hematoxylin and eosin (HE) staining and immunohistochemistry analyses of bone marrow (BM), spleen, and lymph nodes Hematoxylin and eosin (HE) staining and immunohistochemistry analyses of bone marrow ... magnification: ×800) B and C: Hematoxylin and eosin (HE) staining and double immunohistochemistry analyses of BM biopsy (B) and skin biopsy (C) B: HE showed the diffuse infiltration of small lymphocytes ... suppression of the formation of IgM in B cells, the formation of matrix metalloproteinase-3, and the differentiation of osteoclasts [9,10] The bone marrow karyotyping revealed an abnormal karyotype of...

Ngày tải lên: 10/08/2014, 22:20

... successful prediction of NF-κB regulons (a set of genes under regulation of the same TF) in HNSCC cell lines has been achieved by integration of large data sets of gene expression and multiple TFs ... Bradford C, Carey T, et al.: Genetic and expression profiles of squamous cell carcinoma of the head and neck correlate with cisplatin sensitivity and resistance in cell lines and patients Clin Cancer ... genes predicted to be under the regulation of all three NF-κB subunits; 328 genes by RELA; 410 genes by NFκB1; and 306 genes by cREL (Figure 2b and Additional data file 1) In addition, some genes...

Ngày tải lên: 14/08/2014, 08:20

... CD25 and NFAT expression and the AP-1 binding site 141 G Regulatory T cell function, Foxp3 and IL-10 expression and regulation of IL-2 and IFN-γ .142 H Cytokine/Chemokine gene ... Figure 39 Cytokine protein profile of TNF-α and IFN-γ in SB3CT treated CD4+ T cells .107 xvii Figure 40 Cytokine gene and protein expression of IL-2, TNF-α and IFN-γ are reduced in ... results in the expression of genes important for T cell activation including the production of IL-2 In contrast, NFAT activity in the absence of AP-1 activation induces a pattern of gene expression...

Ngày tải lên: 24/08/2014, 11:37

... revealed using molecular cytogenetic techniques and microdissection analysis of HRS cells Mechanism to escape apoptosis is one of the molecular pathogenesis of cHL and signaling pathways involve ... level of L-selectin, moderate level of CD44 and low level of α4 integrin (Picker et al., 1990) In contrast, memory T cells have bimodal expression of L-selectin but definitely higher expression of ... (Weih and Caamano, 2003; Zhu and Fu, 2011) 1.5.3 Role of LT in lymphoid tissue development LT plays a crucial role in lymphoid neogenesis The studies of LT in lymphoid neogenesis and maintenance of...

Ngày tải lên: 10/09/2015, 09:29

... the understanding of the mechanism underlying the pathogenesis of allergic diseases, the relative contribution of various cell types and their products to the initiation and maintenance of allergic ... functional assay of combined LPS and rDer p -pulsed B cells in DO11.10 mice 162-4 165-6 167-8 169-72 173-5 176-7 ix List of Tables Table 1.1 Table 1.2 TLRs, ligands and expression of TLRs on human ... Morphological changes of B cells after stimulation with different doses of LPS Effect of LPS stimulation on B cell’s antigen internalization Expression profiles of co-stimulatory and accessory molecules...

Ngày tải lên: 14/09/2015, 08:27

... microscopy of peripheral blood T-cells shows distinct clustering and segregation of Lck and LAT on the inner leaflet of the plasma membrane [12] These morphological analyses suggest the existence of raft ... BCh-bound and -unbound membranes, at ratios of 10 : and : 2, respectively Lipid rafts are defined as lateral clusters of cholesterol and sphingolipids; however, the biochemical definition of lipid ... method of Fiske and Subbarow [33] Acknowledgements We thank Dr H Waki for technical advice and gifts of authentic gangliosides We thank Dr S Iwashita for critical reading of the manuscript and helpful...

Ngày tải lên: 20/02/2014, 03:20

... type of cystatin and the cancer cell system studied Overexpression of cystatin C has been shown to alter the metastatic properties of B16F10 melanoma cells [9] and to inhibit the motility and ... cytometry and the median of the fluorescence of the cell population was calculated, corrected for the control value and then related to the degree of labelling of the protein used Dashes and rings ... Visualization of acidic compartments by LysoTracker (D) Overlay of B and C, indicating co-localization of cystatin C and LysoTracker B C D taining medium (1 lm) In order to determine the concentration of...

Ngày tải lên: 16/03/2014, 06:20

... selenourea A and selenourea B, may be new candidate antioxidants The generation of a low concentration of O2– in the human body generally plays a beneficial role in biological defense and intercellular ... production of ROS and antioxidant defense mechanisms [1] It is a key component of inflammation and inflammatory disorders The processes associated with inflammatory responses are complex and often involve ... potential of these compounds by examining their scavenging effects on O2– from PMA-stimulated PMNs, with or without inhibition of NADPH oxidase as an index of translocation of p47phox The generation of...

Ngày tải lên: 23/03/2014, 07:20

... Figure dasatinib on (A) invasion and (B) migration in HT144 and Sk-Mel-28 melanoma cell lines Effect of4 Effect of dasatinib on (A) invasion and (B) migration in HT144 and Sk-Mel-28 melanoma cell ... Comparison of IC50 concentrations of temozolomide when tested alone and in combination with dasatinib in HT144, Lox-IMVI, Malme-3M and Sk-Mel-28 cells Standard deviations represent average results of ... the design of the study and carried out the proliferation assays, TUNEL assays, cell cycle assays, Western blotting and statistical analysis JC and MC contributed to the interpretation of the data...

Ngày tải lên: 18/06/2014, 15:20