fixation dehydration and infiltration of bone samples
Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... Rb and Egr-1, in order to determine how the incorporation of EPA and DHA modulate SMC proliferation This article describes the differing effects of n-3 and n-6 PUFAs on the proliferation of SMC ... Effects of n-3 and n-6 PUFAs on the cyclin D1 gene promoter We studied the mechanism(s) underlying the inhibition of cyclin D1 gene expression by EPA and DHA by examining the effects of n-3 and n-6...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
... Rz-1, Rz-2 and with their inactive versions (Rz-1i and Rz-2i) Transfections of the cells with lipofectin (NO) only or lipofectin and an empty plasmid (EM) were used as controls The level of the RT/PCR ... tRNAVal-driven and CTE helicaseassociated hammerhead ribozymes as efficient molecular tools for the inhibition of the biosynthesis of b-secretase and for the exclusion of the formation of toxic b-amyloid ... several months without any decomposition of the RNA Determination of the level of BACE mRNA and ribozyme RNA expression in mammalian cells The level of BACE mRNA and ribozyme RNA was monitored by RT/PCR...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... Each band was analysed densitometrically and the values are depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA (B) Each point is the mean ± SEM of three sets of experiments ... times Cells in the absence of any of these were taken as the control (Con) Two micrograms of total RNA were reverse transcribed with random primers and the levels of PEPCK and G6Pase mRNA were measured ... a series of events beginning with the TNFa-mediated prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis and glucose...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... absence of O2, when the flavin cofactor is reduced [16–19] Thus, under anaerobic conditions in the absence of ammonium, reduction of the flavin moiety of NifL is required to relieve NifL inhibition of ... reduction of purified NifL using artificial electron donors or NADH and (b) analyzed the effect on nif-regulation of different membranebound oxidoreductases of the anaerobic respiratory chain and of terminal ... 280 and 320 nm (MD) Preparation of inside-out vesicles of K pneumoniae One litre cultures of K pneumoniae cells were grown under nitrogen- and O2-limited conditions, harvested at a D600 value of...
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Báo cáo y học: "Differential gene expression of bone anabolic factors and trabecular bone architectural changes in the proximal femoral shaft of primary hip osteoarthritis patients" pptx
... 0.008 and r = 0.70, p < 0.008, respectively; Fig- Comparison of bone structural and turnover indices between OA and control individuals Bone histomorphometry was performed on IT trabecular bone samples ... exploration of any relationships between gene expression and indices of bone architecture and bone remodelling Interestingly, both OCN and ALP gene expression significantly correlated with bone micro-architecture ... investigated changes in gene expression of bone anabolic factors and collagen type I alpha chains and associations between gene expression and bone micro-architecture The bone anabolic factors investigated...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... tissue culture flasks for the inoculation of virus isolate at a multiplicity of infection (m.o.i.) of one After hour of adsorption, the virus was removed and ml of fresh RPMI-1640 media with 2% FBS, ... of H9N2/1997 only showed a transient elevation at hours post-infection In contrast, the elevation of TGF-b2 level of H1N1/2002 was sustained and increased with time reaching 2-3 folds at 18 and ... [35-37] The mechanism concerning bronchial infiltration of inflammatory cells, particularly lymphocytes and eosinophils, and the subsequent hyperresponsiveness of the bronchial wall induced by viral...
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Báo cáo y học: "Common gene expression strategies revealed by genome-wide analysis in yeast" ppt
... also found Because TLR is the product of TLRi and RA, this can be the result of the negative correlation of RA and RS and the lack of correlation between TLRi and RS However, no correlation Genome ... blue) between RA and PA, PA and TLR or TLRi, RA and TR and between TR and TLR or TLRi Some of these correlations have been described previously [8,11,17,19] The correlation between TR and TLR was ... sizes were equal to that of genes in each considered group and subsequent computation of average and standard deviations for each variable An estimation of the average standard deviations (aSE)...
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Báo cáo sinh học: "Localization of leucocyte interferon gene in the q2.5 region of pig chromosomel by in situ hybridization" potx
... probe RESULTS AND DISCUSSION A total of 69 well-banded chromosome spreads were scored Among 579 grains counted on the chromosomes (Fig 1), 500 (86%) were observed on chromosome and out of the 500 ... locate the silver grains and identify the chromosomes The chromosomes were classified according to the G-band pattern, defined by the Committee for the standardized karyotype of the Domestic Pig (1988) ... the presence of a 1,000-fold excess of sonicated salmon sperm DNA The precipitate was diluted in the following solution: 50% ionized formamide; 10% dextran sulfate; and 1/5 volume of SCCP (0.2...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... other (in case of lymphoma h1) and SMG-1, the gene of ribosomal protein S8 (in case of lymphoma h2), as well as several genes of unknown function (9 and in the case of lymphoma h1 and h2, respectively) ... analysis of the transcription levels of the set, ND4, and SMG-1 genes in human spontaneous lymphomas and normal B- and Tlymphocytes (a) 32P-labeled PCR-fragments of the set and a-myb oncogenes, and ... cDNA population of lymphoma h1 as tracer, and the cDNA population of lymphoma h2 as driver; and vice versa, 2) the cDNA population of lymphoma h2 as tracer, and the cDNA population of lymphoma h1...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... understanding of the influence of the molecular function is necessary, as well as of biochemical processes and of the biophysical properties provoked amongst others by a moderate repression of the ... stringency controls, a successful staining of the test samples, clear negative controls and noise, and an expected expression of housekeeping genes The samples were very homogeneous (figure 2) ... of microarray data after h and 24 h application of the transport peptides The figure shows the graph of the sample correlations of the raw microarray data The time groups are highly similar Samples...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
... representative TEM images of mitochondria in eWAT (Figures 2A and 2B) and iBAT (Figures 2C and 2D), respectively, and the analyses of mitochondria number (Figure 2E) and area (Figure 2F) In the ... The number and area of mitochondria in the eWAT and iBAT A-B Representative TEM images of eWAT C-D Representative TEM images of iBAT (Arrows point to mitochondria) E: The analysis of mitochondrial ... on our understanding of the adverse effects of particulate air pollution on cardiometabolic diseases, especially in the context of obesity and insulin resistance Xu et al Particle and Fibre Toxicology...
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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
... date, four isoforms of PDK (PDK1–4) and two isoforms of PDP (PDP1–2) have been identified in humans [3–5] These isoforms display unique tissue distribution [3–5] and varied kinetic and regulatory ... Regulation of hPDK2 and hPDK4 gene expression Table The effects of PPAR a, d and c agonists on PDK2 and PDK4 transcript abundance expressed as a percentage of basal levels Results are the means ± SEM of ... 50 pmol of each gene-specific primer, ng of double-stranded cDNA, dNTPs (200 lm), buffers and 0.5 U of Expand High Fidelity Polymerase (Roche Diagnostics Ltd, Lewes, UK) in a final volume of 100...
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Báo cáo khoa học: Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana ppt
... mixture of the two types of probes For analysis of differential gene expression, 1473 and 1703 beads were collected in fractions U1 and U2 of the up-regulated genes, and 3550 beads in fraction D of ... some of them might be missorted at the gates, which would expand the list of genes Of the beads collected at gates U1 and D on fluid microarray analysis, 89 and 87% were regarded as up-regulated and ... incubated in the presence (lanes and 4) or absence of TM (lanes and 2) for 48 h Proteins were extracted, digested with (lanes and 4) or without (lanes and 3) Endo H, and then subjected to SDS ⁄ PAGE...
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Báo cáo khoa học: Wild-type p53 enhances annexin IV gene expression in ovarian clear cell adenocarcinoma docx
... Protein and total RNA were extracted from various EOC cell lines, HEK293 and LNCaP cell lines (A, B) Expression levels of protein and mRNA, and levels of p53, ANX4 and the known p53 targets, p21 and ... Protein and RNA were extracted from two CCA (OVTOKO and OVISE) cells lines and one non-CCA (MCAS) cell line, and then ANX4 protein (A) and mRNA (B) levels were compared by western blotting and real-time ... HEK293 and LNCaP cell lines expressed trace amounts of ANX4 (Fig 5) Expression levels of p21 or MDM2 are higher in CCA p53 is a positive regulator of annexin IV cell lines than those of HEK293 and...
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Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes potx
... differences in mRNA levels of four enzymes of the urea cycle Whereas the zonal-specific expression of the main enzymes of drug and xenobiotic metabolism has been the subject of extensive research (e.g ... Expression profiles of perivenous and periportal hepatocytes Perivenous and periportal mouse hepatocyte fractions were obtained by combined digitonin ⁄ collagenase perfusion of liver of male C3H ⁄ ... cells, code for 6-phosphofructo-2-kinase, which produces fructose-2,6-bisphosphate, an allosteric activator of the glycolytic enzyme 6-phosphofructo-1kinase and an inhibitor of the gluconeogenic...
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Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt
... B1 isoform) of other insect EcRs and a strong degree of identity was confined to the two ends of N-terminal region of the A/B domain Using a B1 isoform-specific probe from the A/B region of PIEcR, ... induction of mRNAs, the level of PIE75-B, PHR3 and PIEcR-B1 mRNAs was assessed after 1, and 18 h exposure, respectively Fig 8B and C show that after h and h exposure to 20E, the amount of accumulated ... induction level of PHR3 mRNA, restore the expression of A and B cyclins and consequently prevent G2 arrest [41] Our study confirmed the effect of JH II on the 20E inducibility of PHR3 and revealed...
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Báo cáo khoa học: Effect of ecdysone receptor gene switch ligands on endogenous gene expression in 293 cells docx
... total of 20 lL reaction volume containing lL of cDNA, lL of each of forward and reverse sequence specific primers (from 10 lm primer stock), 10 lL of supermix (Bio-Rad Laboratories) and lL of nuclease ... ecdysteroids and ecdysone mimics can induce and ⁄ or suppress endogenous genes in RKO and other mammalian cells and promote apoptosis A more comprehensive understanding of possible pleiotropic effects of ... effects of EcR switch inducers in mammalian cells We studied the effect of eight EcR ligands: four diacylhydrazines, two ecdysteroids, one THQ and one AMK ligand (Fig 1) on the expression of endogenous...
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Báo cáo hóa học: "Prognostic Impact of MiR-155 in Non-Small Cell Lung Cancer Evaluated by in Situ Hybridization" pot
... analysis and drafted the manuscript KE, TB and KL carried out and supervised the ISH SWS and KE scored the cores KAS, SAS, SA and HS contributed in the clinical and demographic database and KAS and ... 2Institute of Clinical Medicine, University of Tromso, Tromso, Norway 3Department of Pathology, University Hospital of North Norway, Tromso, Norway 4Institute of Medical Biology, University of Tromso, ... (SCC) and negative lymph node status (N0); (B) SCC and positive lymph node status (N+); (C) adenocarcinomas (AC) and N0; (D) AC and N+ Author details Department of Oncology, University Hospital of...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
... μl of the 2X QuantiTect™ SYBR® green master mix, 0.1 μl of HK-UNG (Epicentre, Madison, WI), and μl of RNase-free water for each sample for a total volume of 20 μl The PCR profile consisted of ... observed in the HP content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of HP/mg of tissue wet weight Differential ... observed in the GAG content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of GAG/mg of tissue wet weight...
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Báo cáo hóa học: " Altered gene expression in asymptomatic SHIV-infected rhesus macaques (Macacca mulatta)" pdf
... significant subset of the genome of interest as a regulator of cell growth and apoptosis SIV infection results in down-regulation of apoptosis inhibitor (API5, ID: AI972925) and up-regulation of pro-apoptotic ... potential to greatly expand the understanding of SHIV-host interaction beyond the limited number of cell types or cytokines generally examined In animals free of clinical signs of SHIV, altered baseline ... pathogenesis of cognitive and related ailments arising with HIV infection Results Clinical history A brief description of treated and control animals is given in Table All monkeys were male; while two of...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25