fetax assay for evaluation of developmental toxicity

... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... Analysis of HA and quantitative real-time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real-time PCR data employed for the determination of JC ... was used for PCR in a final volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension...

Ngày tải lên: 19/06/2014, 08:20

... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... Analysis of HA and quantitative real-time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real-time PCR data employed for the determination of JC ... was used for PCR in a final volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension...

Ngày tải lên: 20/06/2014, 04:20

... fields) Speed of detection of resources Number of nectar and pollen foragers Time and frequency of foraging trips Number and proportion of eggs, larvae, pupae and adults per season Life span of adults ... reaches, for example, 95% of the density of the control simulations While the use of a threshold may seem to be an easy and straightforward approach, the amount of effect being acceptable will vary for ... knowledge of the ecology of a species affects not only the realism of model simulations, but also of higher tier risk assessments or the use of field studies in risk assessment Therefore, for a detailed...

Ngày tải lên: 20/06/2014, 20:20

... fields) Speed of detection of resources Number of nectar and pollen foragers Time and frequency of foraging trips Number and proportion of eggs, larvae, pupae and adults per season Life span of adults ... reaches, for example, 95% of the density of the control simulations While the use of a threshold may seem to be an easy and straightforward approach, the amount of effect being acceptable will vary for ... knowledge of the ecology of a species affects not only the realism of model simulations, but also of higher tier risk assessments or the use of field studies in risk assessment Therefore, for a detailed...

Ngày tải lên: 20/06/2014, 20:20

... fields) Speed of detection of resources Number of nectar and pollen foragers Time and frequency of foraging trips Number and proportion of eggs, larvae, pupae and adults per season Life span of adults ... reaches, for example, 95% of the density of the control simulations While the use of a threshold may seem to be an easy and straightforward approach, the amount of effect being acceptable will vary for ... knowledge of the ecology of a species affects not only the realism of model simulations, but also of higher tier risk assessments or the use of field studies in risk assessment Therefore, for a detailed...

Ngày tải lên: 21/06/2014, 17:20

... from g sample of PMMA for heat-cure denture base material 10 Min Figure 2: Residual MMA diagram collected from g sample of PMMA for self-cure denture base material HPLC Analysis for Evaluation ... properties of denture base material related to the effect of level of residual monomer, and concluded that the percentage of higher levels of residual monomer effected on the tensile properties of denture ... from injection of the sample as shown in the diagram The average of 12 readings of each sample of heat-cure and self-cure denture base materials were shown in Table The results of MMA content...

Ngày tải lên: 07/08/2014, 14:20

... extravascular-extracellular space of the tumor are also the most likely mechanisms responsible for the transient increase in TV Obviously, the timing of the evaluation of tumor response after the start of treatment ... the level of statistical significance Results There was an excellent interobserver agreement between the two readers with a kappa coefficient of 0.93 for the assessment of TV and 0.89 for ADC values ... issue For the present study, we chose to perform MRI including DWI very early at a median of days following HDRBT Thus, we were enabled to obtain first information on the treatment response before...

Ngày tải lên: 09/08/2014, 09:20

... the maximum angle for each of three trials was identified for each direction of movement The average of the three trials was used as outcome measure for maximum active range of motion in each ... software was used for precise calculation of repeated angle measurements from gait [21] The precision of angle measurements for the cervical spine using the Plug-in gait modeling software has not ... whiplash trauma Failure of conservative treatment for more than one year Absence of gross neurologic signs Absence of gross radiological (MRI) pathology length), and cervical range -of- motion (degrees...

Ngày tải lên: 10/08/2014, 10:20

... procedure is a combination of the time needed for preparation of a patient, time needed for preparation of an instrument, actual time of a procedure, and the time needed for data interpretation ... proposed for SEMG evaluation of dysphagia and odynophagia SEMG evaluation of deglutition is not a new diagnostic method Nevertheless, lack of standard requirements decrease the outcome of this ... SEMG usage in evaluation of swallowing process While the normative database for SEMG assessment of deglutition has been established, the next step might be establishment of standards for this diagnostic...

Ngày tải lên: 11/08/2014, 20:20

... the plaque assay Real-time PCR The quantitative CMV real-time PCR assay is based on detection of a 151bp region from the highly conserved US17 gene [20] The limit of detection of the assay is 100 ... of dH20, and 10 μl of template Amplification was performed on a 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) PCR conditions were: 50°C for min, 95°C for 10 min, 40 cycles of ... reporter assay for determination of CMV inhibition by anti-viral agents The assay, based on pp28-luciferase recombinant CMV, can be performed 72 hpi and drug treatment, has a large dynamic range of...

Ngày tải lên: 11/08/2014, 21:21

... evaluation of concrete 6.2.4.1 Evaluation of portions of structures not requiring immediate repair 6.2.4.2 Evaluation of portions of structures requiring immediate repair 6.2.4.3 Alternative methods of ... chemical analysis for any of many reasons, the most common is for determination of the proportion of cement used in the mixture ASTM Method C 1084 and variants of this are usually employed for this purpose ... Concrete 224.1R Causes, Evaluation, and Repair of Cracks in Concrete Structures 228.1R In-Place Methods for Determination of Strength of Concrete 437R Strength Evaluation of Existing Concrete Buildings...

Ngày tải lên: 24/10/2014, 17:26

... increased use of NDT methods is occurring despite the lack of testing standards for many of the methods The development of testing standards is critical for proper application and expanded use of NDT ... Ultrasonic-echo method—Some of the drawbacks of the through-transmission method are the need for access to both sides of the member and the lack of information on the location (depth) of a detected anomaly ... mm, the method is best suited for measurement of the surface zone of a concrete element A good example of the use of this method is the monitoring of the density of bridge deck overlays Non-contacting...

Ngày tải lên: 24/10/2014, 17:44

... STRATIFICATION STRUCTURAL PERFORMACE UNIFORMITY OF CONCRETE 00 0 l 0 l 00 l l 364.1R-13 EVALUATION OF STRUCTURES PRIOR TO REHABILITATION Table 6.2 -Evaluation of properties of reinforcing steel PROPERTIES ... process of reestablishing the materials, form, and appearance of a structure to those of a particular era of the structure Strengthening-The process of increasing the load-resistance capacity of a ... the evaluation The evaluation work is generally performed for one or several of the following purposes: a) To determine the feasibility of changing the use of a structure or retrofitting the structure...

Ngày tải lên: 24/10/2014, 21:59

... provide important information of the localization of epileptic foci for guiding surgical decisions The technique has been validated and extensively used for the pre-surgical evaluation of patients suffering ... and the depth of dipole 65 6.3.4 Linearity check 66 6.4 Concluding Remarks 66 CHAPTER 68 Evaluation of the Performance of LORETA Method Utilized for Deep Brain ... electrical property and evaluation of the performance of utilizing the head model for EEG studies, the subcortical emphasized physical head model was finally developed The novelty of this head model...

Ngày tải lên: 09/09/2015, 10:02

... APPLICATION OF A HIGHTHROUGHPUT SCREENING ASSAY FOR DISCOVERY OF STARCH HYDROLASE INHIBITORS LIU TING TING (BSc, MSc Wageningen University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... Determination of IC50 value of grape seed extracts - 115 7.2.3 Determination of grape seed extracts total phenolic content - 116 7.2.4 Determination of proanthocyanidin profile of grape seed ... of the proanthocyanidin profiles of grape seeds extracts - 120 7.3.3 Determination of IC50 values of the grape seed extracts - 123 7.3.4 Formulation of grape seed wheat noodles...

Ngày tải lên: 10/09/2015, 09:01

... screening  assays  for inhibitors  of PCV2  replication.      Cell‐Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication        Page | 5         List of Tables and Figures  ... positive controls, is used to measure assay performance and reproducibility because it considers  variability among measurements and is amenable  for comparing assays and screens of various  formats  not  performed  simultaneously  ... assay for detecting PCV2 replication had to be developed. In line with these, the main goals of the study were:  ‐ Development  of a  primary  screening  method  for PCV2  replication  in  96‐well  and  384‐well plate format  ‐ Performing a POC trial for inhibition of viral replication using reference compounds ...

Ngày tải lên: 03/10/2015, 11:36

... allocation of resources from the point of view of society National parameters used for the purposes of project evaluation are a numerical expression of limits of acceptability from the point of view of ... benefits for a region of a country 93 Distribution of the net domestic value added 97 Foreign-exchange flows of a project 99 Impact of a set of projects on the balance of payments 99 Net foreign-exchange ... needed for project evaluation as possible This Manual suggests a set of model formats for the information most necessary for project evaluation Indeed, a project's feasibility in terms of its...

Ngày tải lên: 04/10/2015, 20:12

... infection rates, but instead resulted to greater cell death. The number of cells attached to the bottom of the plate 24 hours post‐seeding was significantly less than the number of attached cells in plates seeded  Cell‐Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication     ... and  96‐well  plate  formats  the  infection  rates  failed  the  minimum  target  of 50%,  the  IFA  format  was  abandoned  and  efforts  were  turned  towards  an  assay format  that  didn’t  ... materials  had  been  obtained,  development  of the  screening  assay was  performed. Detection of PCV2 infection and replication through IFA of Rep protein is a well‐established  method ...

Ngày tải lên: 13/10/2015, 16:41