factors interleukins collagen ecm modulators and cell types involved in wound healing differ between fetal and adult wounds
A novel role of hydrogen sulfide in wound healing and a new approach to wound dressing in rat model
Ngày tải lên :
26/09/2015, 09:39
... role of H2S in skin wound healing in rats Dermal wound healing is a complex phenomenon involving inflammation, re-epithelialization, granulation tissue and tissue remodeling Square wounds (15 ... the wound healing process H2S synthesizing activities in liver and skin for both wounded and non-wounded animals were examined [Fig.4.2] - 45 - Chapter H2S synthesizing activity in wounded and ... administration on MPO activity in wound healing We measured the fold increase in MPO activity in control animals after wounding In vehicle pretreated animals, surgery induced wounding caused inflammation,...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there ... Th1 cells produce interferon-c (IFN-c) and lymphotoxin, activating cell- mediated immunity and providing protection against intracellular pathogens and viruses Th2 cells secrete interleukin-4 ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a...
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Báo cáo khoa học: Acute activation of Erk1/Erk2 and protein kinase B/akt proceed by independent pathways in multiple cell types ppt
Ngày tải lên :
30/03/2014, 20:20
... in multiple cell types, beginning with FDC-P1 cells, to determine whether our initial results were limited to a particular cell line Cytokine-starved FDC-P1 cells were treated with PtdIns3K inhibitors, ... PLCc and PKC are also intimately involved in the pathway leading to Erk activation in B cells [36] Effects of PtdIns3K inhibitors in HEK293 cells We also wanted to examine nonhemopoietic cells ... pathway was indeed the more dominant in determining cytokinedependent survival of cells Thus, we compared the apoptosis of the hemopoietic cells in the presence of PtdIns3K and MEK inhibitors...
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Báo cáo khoa học: " Targeting lentiviral vector to specific cell types through surface displayed single chain antibody and fusogenic molecule" pps
Ngày tải lên :
12/08/2014, 04:21
... efficiency Incorporation of SCAb and FM onto lentiviral vectors A virus -cell binding assay was performed to evaluate SCAb-mediated binding to CD20-expressing cells As a target, we used a 293T cell line ... to the control 293T cell line In contrast, the lentiviral vector FUW/VSVG was able to bind to both 293T and 293T/ CD20 cell lines In addition, a quantitative virus -cell binding assay was conducted ... CD20-expressing cells We also compared the targeted transduction efficiency and the binding avidity of both versions of the SCAb and investigate the molecular roles of the displayed proteins in mediating...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
Ngày tải lên :
11/09/2015, 16:02
... enzymes and cellular machinery required to process Lefty2 are present in ES cells and assures us that Lefty2 processing is indeed active in ES cells and that mature Lefty2 proteins are secreted into ... candidate since i) Lefty2 expression can be detected in pluripotent cell types in vivo such as the inner cell mass and the epiblast 88 ii) Lefty2 is highly expressed in undifferentiated ES cells ... Lefty1 and Lefty2 in ES cells are overlapping Upon ascertaining that Lefty keeps ES cells undifferentiated, it is important to next determine if silencing of Lefty2 can promote differentiation into...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
Ngày tải lên :
11/09/2015, 16:02
... Activin A ligand causes differentiation of ES cells into meso/mesendodermal lineages while repression of signaling with the specific chemical inhibitor SB431542 and Lefty protein supplementation induces ... Nodal signaling might, as in the in vivo scenario, likewise influence ES cell fate decision in a dose dependent manner in vitro is addressed Interestingly, it was found that Nodal signaling is able ... dictate embryonic stem cell fates is also not entirely clear Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation have both...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
Ngày tải lên :
11/09/2015, 16:02
... were maintained on uncoated cell culture dishes in ES cell medium containing DMEM/20% ES FBS (Invitrogen, Carlsbad, CA), 0.1 mM MEM nonessential amino acids (Invitrogen), 2mM L-glutamine (Invitrogen), ... days growth in selective medium, few cells remained in the untransfected control wells indicating that selection was effective The surviving cells were trypsinized and resuspended in ES cell medium ... Protein extraction and western blotting To obtain protein extracts, cells were trypsinized from culture dishes harvested in chilled PBS, centrifuged at 10,000g for at °C, washed again in PBS and incubated...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
Ngày tải lên :
11/09/2015, 16:02
... neural and metabolic defects and results in perinatal death Development 121, 1283-1299 Goldin, S N., and Papaioannou, V E (2003) Paracrine action of FGF4 during periimplantation development maintains ... Maeda, M., and Yamanaka, S (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells Cell 113, 631642 Moffat, J., and Sabatini, D M (2006) Building mammalian ... T., Niwa, H., and Miyazono, K (2007) Activin-Nodal signaling is involved in propagation of mouse embryonic stem cells J Cell Sci 120, 55-65 Oh, S P., and Li, E (1997) The signaling pathway mediated...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
Ngày tải lên :
11/09/2015, 16:02
... level during pluripotency and is almost absent in terminally differentiated cell types (Levine and Brivanlou, 2006) In fact, Lefty molecules are the most abundant inhibitors both in mouse and human ... 6bromoindirubin-3'oxime (BIO) It was demonstrated that pluripotency can be maintained in both mouse and human ES cells in the presence of this inhibitor Interestingly, Lefty A and Lefty B are inducible ... crucial for maintaining ES cells in an undifferentiated state 1.2.1 Extrinsic regulators and signaling pathways governing ES cell self renewal 1.2.1.1 LIF/gp130/STAT3 pathway When mouse ES cells were...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
Ngày tải lên :
11/09/2015, 16:03
... teratocarcinoma Differential expression pattern between ES cells and differentiated cells Reduced expression during retinoic acid induced differentiation of F9 cells High expression in ES cells Link to ... protein Member of TGFsuperfamily Involved in Nodal signaling Nodal antagonist • • • • • Rex2 • Zinc finger protein • • • Zfx • • • a-catenin • Zinc finger protein Transcription factor Link to ... Oct4 and Nanog target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells...
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Factors influencing borrower’s behavior and decision making patterns in the success of a micro finance model
Ngày tải lên :
06/09/2013, 05:48
... micro financing by realigning their marketing mechanisms and strategies to attract less educated masses through staff trainings and holding open sessions in these localities and persuading them in ... remaining market share in micro financing After reviewing the literature on the positive impact of micro financing in developing countries like Bangladesh, Zambia and Bolivia, it can be inferred ... attracted to debt financing On the other hand, the individual with low income and savings is more attracted towards debt financing The selection of income group and the means of income are very...
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Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt
Ngày tải lên :
18/02/2014, 04:20
... DNA-binding factors, including HSF, as well as general transcription factors, cannot bind to nucleosomal DNA [50] In cells, there are various proteins and protein complexes that change chromatin ... constitutively in cells and binds to and regulates the expression of genes without any apparent stress intervention [1,3] HSF2 and HSF4 may not be directly involved in the stress response, but rather in cell ... DBD–DBD interaction is important in the HSF–HSF and HSF–HSE interactions In HSF DBD– HSE co-crystals, the protein–protein interface consists of the helix amino-terminus, the turn and the wing [15]...
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Tài liệu Báo cáo khoa học: Diego and friends play again Old planar cell polarity players in new positions doc
Ngày tải lên :
20/02/2014, 01:20
... PCP in the Drosophila wing and eye In Drosophila, tissue polarity has been studied in a number of different body regions but it is been best understood in the wing and eye In the wing, each cell ... nonautonomous polarity phenotypes To explain this finding, they propose that Fj most likely acts by modulating the activity of other proteins involved in intercellular signaling The best candidates as targets ... Fritz (Frtz) or both are involved in In recruitment and localization This would be consistent with several different findings First, asymmetric In accumulation and In protein stability have both...
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Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc
Ngày tải lên :
07/03/2014, 15:20
... cultures and in developing tissues provided interesting insights into the function of eIF4B In mammalian cell cultures, a 50-fold overexpression of eIF4B resulted in the inhibition of the protein synthesis ... of all cell types in the Drosophila eye Cell 87, 651–660 52 Kimmel, B.E., Heberlein, U & Rubin, G.M (1990) The homeodomain protein rough is expressed in a subset of cells in the developing Drosophila ... containing 10% fetal bovine serum and incubated for 24 h or an additional 48 h The number of viable cells at the time of re-plating (0 h) is taken as 100% (D) Protein synthesis in fed (10% fetal...
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Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
Ngày tải lên :
08/03/2014, 22:20
... appears to be independent of the cell type, and thus of cell- specific protein interactions MATERIALS AND METHODS Cell lines The rat IR938F, a pre-B cell lymphoma, and the human Jurkat cell line, derived ... proteins containing the GAL4 DNAbinding domain and the c-Jun activation domain (residues 1–223) in wild-type and mutant forms (not phosphorylatable by mutation of both serines 63 and 73 to alanine) ... Co-stimulation of T cells with CD2 augments TCR-CD3-mediated activation of protein tyrosine kinase p72syk, resulting in incresed tyrosine phosphorylation of adapter proteins, Shc and Cbl Int Immunol...
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Báo cáo khoa học: Molecular basis for specificities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases pptx
Ngày tải lên :
16/03/2014, 05:20
... enzymebound, adenine-lacking cobalamins for free adenosylcobalamin, an adenine-containing cobalamin through intermediary formation of apoenzyme The function of reactivating factors is to release ... reactivating factors in the reactivation of inactivated holoenzymes The specificities of reactivating factors in the in vitro reactivation of inactivated holoenzymes were investigated using purified ... cofactor and CN-Cbl, respectively, for free intact coenzyme CN-Cbl and AdePeCbl can be considered models of the damaged cofactor (adenine-lacking cobalamin) and intact coenzyme (adenine-containing...
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Báo cáo khoa học: Distinct but critical roles for integrin aIIbb3 in platelet lamellipodia formation on fibrinogen, collagen-related peptide and thrombin pot
Ngày tải lên :
16/03/2014, 12:20
... conformational change in the extracellular domain, leading to an increase in affinity, thereby promoting integrin–ligand interactions [2] In turn, clustering of integrins generates a series of intracellular ... ligand in vivo [13] In epithelial cells, it has been suggested that immobilized thrombin can bind to integrins through an RGD site, raising the possibility that is may bind directly to integrins ... critical role of integrins in mediating adhesion and cell spreading To address this, experiments were designed to compare the role of the major platelet integrin aIIbb3, in supporting spreading on the...
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Báo cáo khoa học: Differing molecular mechanisms appear to underlie early toxicity of prefibrillar HypF-N aggregates to different cell types potx
Ngày tải lên :
16/03/2014, 13:20
... the different response of either cell line to the same toxic insult can be traced to, among others, a differing interaction between the aggregates and the plasma membrane, resulting in differing ... toxicity: new insights into protein folding, misfolding diseases and biological evolution J Mol Med 81, 678–699 Dobson CM (2004) Principles of protein folding, misfolding and aggregation Semin Cell Dev ... in HypF-aggregate-treated cells higher than in controls, increasing slowly between and 24 h In contrast, in IMR90 cells the Ca2+ increase was much smoother and smaller, and without an early peak...
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Environmental Health Sciences to develop and operate multidisciplinary research centers regarding the impact of envi- ronmental factors on women''''s health and disease prevention pptx
Ngày tải lên :
22/03/2014, 11:20
... basic and clinical research; ‘‘(B) develop protocols for training physi- cians, scientists, nurses, and other health and allied health professionals; ‘‘(C) conduct training programs for such individuals; ... the Institute 22 ‘‘(b) RESEARCH, TRAINING, AND INFORMATION AND 23 EDUCATION.— 24 ‘‘(1) IN GENERAL.—Each center under sub- 25 section (a) shall, with respect to the purpose de- 26 scribed in such ... continuing education programs for such professionals; and ‘‘(E) disseminate information to such pro- 10 fessionals and the public 11 ‘‘(2) PRIORITY FOR PREVENTION ACTIVITIES.— 12 In carrying...
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SMALL EMPLOYER HEALTH TAX CREDIT: Factors Contributing to Low Use and Complexity doc
Ngày tải lên :
22/03/2014, 20:20
... example, IRS modified its outreach from initially focusing on tax preparers and small employers to including insurance brokers in 2012 Addressing Factors and Expanding Credit Use May Require Substantive ... calculations (FTE and wage phase out) Source: GAO analysis of IRS information Print instructions To view and print full noninteractive versions of IRS Form 8941 and worksheets included in this graphic, ... have minimum wage laws and minimum wages vary from state to state See Quantria Strategies/Small Business Administration, Health Insurance in the Small Business Market: Availability, Coverage, and...
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