f g from in vitro data

... activity is far less clear (e .g Fig in ref 142) Indeed, further purification of such extracts by gel filtration chromatography resulted in fractions containing hexokinase in which the presence of VDAC ... contradictory effects of PBR ligands can be rationalized on the basis of differences in cell type, additional confusion arises from studies showing that individual PBR ligands can have different effects ... and gating in which the pore forms by aggregation of misfolded integral membrane proteins damaged by oxidant and other stresses [186], which is reminescent of an earlier model suggesting that...

Ngày tải lên: 30/03/2014, 11:20

... mouse egg fertilized in vitro Nature 260, 39-40 Ho PC, Yeung WS, Chan YF, So WW, Chan ST (1994) Factors affecting the incidence of polyploidy in a human in vitro fertilization program Int J Fertil ... the incidence of diploid secondary oocytes A technical artefact (the mixing of chromosomes originating from different blastomeres during slide preparation) cannot be excluded REFERENCES Angell ... either in vivo or in vitro was quite high Moreover the incidence of embryos carrying anomalies in the ICM did not differ significantly from those of entire embryos Most chromosome aberrations found...

Ngày tải lên: 09/08/2014, 18:22

... Simonsson S, Gurdon JB: Changing cell fate by nuclear reprogramming Cell Cycle 2005, 4:4 Handyside AH, Lesko JG, Tarin JJ, Winston RM, Hughes MR: Birth of a normal girl after in vitro fertilization ... which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines will promote the sanctity of life "without undermining it" and ... obtained for PGD could be maintained in culture specifically for ESC production As with traditional PGD protocols, genetic data needed from PGD could still be obtained and inform embryo transfer decisions,...

Ngày tải lên: 13/08/2014, 23:20

... scaling exponent from LSLR Table lists scaling exponents from BMR prediction using body-weight data from Heusner [10] and from Kleiber [1,3] Parameters used for the predictions are MLEs from fitting ... log(BMR) expressed as a function of log(M) has a slope that is strictly increasing This can be seen by writing the sum of power functions in Equation (3) in order of increasing magnitude of the ... using MLEs of parameters calculated by fitting the White and Seymour data, scaling exponents from LSLR of predictions based on body-weight data of Kleiber are greater that those from LSLR of...

Ngày tải lên: 13/08/2014, 23:20

... ConfigurationSettings.AppSettings["Sql_ConnectString"]); DataTable dt = new DataTable("Orders"); da.FillSchema(dt, SchemaType.Source); CreateTableFromSchema(dt, ConfigurationSettings.AppSettings["Sql_ConnectString"]); ... "[nchar] (" + maxLength + ")"; break; case "System.String": if(maxLength == 0x3FFFFFFF) sqlType = "[ntext]"; else sqlType = "[nvarchar] (" + maxLength + ")"; break; case "System.Single": sqlType = ... Make the mapping decisions based on the requirements of your application The mapping method also adds the field length for the DDL column description for string-type columns For more information...

Ngày tải lên: 21/01/2014, 11:20

... of the form you are calling in the URL Format String so that you can name it the same in step Add the code in Listing 5.30 to the Load event of the page Listing 5.30 wfrmHowTo5_8a.aspx.vb: Filling ... solution From the main page, click on the hyperlink with the caption How-To 5.8: Hyperlink From a Row in the Data Grid to a Detail Page You then see all the products loaded into a data grid Notice ... How-To for editing data Using data with your Web Forms is not much harder than using Windows Forms Just remember to stash some variables for round trips to the server and to bind your data ...

Ngày tải lên: 21/01/2014, 12:20

... GTGATTCATGGTGGAAATACGCCCCCATCAGGGGGCTGG TTTTGCGGCCGCGCCAGCCCCCTGATGGGGGCGCAGCCTCCAGGA CCCCCCCTCCCGGGAGAGCCATAGTGGTCTGCGGAACCGGTTTT AAAAACCGGTTCCGCAGACCACTATGGCTCTCCCGGGAGGGGGGG TCCTGGAGGCTGCGCCCCCATCAGGGGGCTGGCGCGGCCGCAAAA ... TTTGCGGCCGCGCCAGCCCCCTGATGGGGGCGACACTCCACCATGAATTCT AGCCATGGTTT AAACCATGGCTAGAATTCATGGTGGAGTGTCGCCCCCATCAGGGGGCTGGC GCGGCCGCAAA GGCTGCACGACACTCCGCCATGGCTAGACGCTTTC CGTCTAGCCATGGCGGAGTGTCGTGCAGCCTCCAGG CCCCTGATGGGGGCGTATTTCCACCATGAATCACTCCCC ... DHp2s GCCAGCCCCCTGATGGGGGCGA TAATACGACTCACTATAGGGTGCACGGTCTACGAGACCT GCCAGACACTCCACCATGAATCACTCCCCTGTGAGGAACTACTGTCTTCACG TAATACGACTCACTATAGGGTGCACGGTCTACGAGACCT TTTGCGGCCGCGCCAGCCCCCTGATGGGGGCGACACTCCACCATGAATTCT...

Ngày tải lên: 20/02/2014, 01:20

... forward primer (A) 5¢-TCTACCCACCCAACGAAGGCT-3¢ (nucleotides 1007–1027), SR-BI reverse primer (B) 5¢-CCTGAATGGC CTCCTTATCCT-3¢ (1514–1534) and SR-BII reverse primer C-5¢-AGAAGCGGGGTGTAGGGACTGG-3¢ ... protein fraction from ldlA(mSR-BI) cells at a molar ratio of : 20 prevented antibody binding, demonstrating that staining was specific for SR-BI The lack of SR-BI expression in BeWo cells (Fig 3C) ... RNA-easy kit (Qiagen) exactly as described [40] A 553-bp fragment, amplified by RT-PCR [forward primer 5¢-TCGCTCAT CAAGCAGCAGGT-3¢ (nucleotides 169–188), reverse primer 5¢-GCCCAGAGTCGGAGTTGTTG-3¢ (nucleotides...

Ngày tải lên: 20/02/2014, 23:20

... plan of laying their eggs in the ground that is still good enough for the petrels, penguins, kingfishers, and many other kinds Protection of the eggs from rain, frost, and natural enemies suggests ... abracadabra of the mathematical biologist, and just to give thanks for a sight so inspiring as that of gulls ringing high in the eye of the wind over hissing combers that break on sloping beaches ... particularly in estuaries and where the coast is rocky, gulls are a familiar sight in the wake of steamers at the beginning and ending of the voyage, as well as following the plough and nesting in the...

Ngày tải lên: 06/03/2014, 13:20

... oxidized glutathione (GSSG) and even slightly tighter metal binding under the in uence of reduced glutathione (GSH) [37] We investigated in vitro the effect of GSH/GSSG on NOmediated metal release of ... compound(s) being involved in this interaction in living cells and information about this additional factor is needed in order to perform physiologically relevant future in vitro studies and in the interpretation ... tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain In addition to the already described differences in flexibility of the b-domain in MT...

Ngày tải lên: 07/03/2014, 15:20

... primers 5¢-GCGGAGCTCATGGCCAC AAGCGCATCAGC-3¢ and 5¢-GTGGTGGTGGTGGTGG TGGAAATGGGTTTTTCCGTTCTGC-3¢ (His-tag underlined) or 5¢-TGGAGCCACCCGCAGTTCGAAAAAGAA GCTGGAGATGATCGTGCT-3¢ (Strep-tag underlined), ... designed as follows: PSAG with a C-terminal hexa-histidine tag (PSI -G- HisTerm), 5¢-GCGGAGCTCAT GGCCACAAGCGCATCAGC-3¢ and 5¢-GCGGCATGCT CAGTGGTGGTGGTGGTGGTGTCCAAAGAAGCTTG GGTCGTAT-3¢ (His-tag underlined); ... CACCACCACCACGAAGCTGGAGATGATCGTGCT-3¢ (His-tag underlined) or 5¢-TGGAGCCACCCGCAGTTCG AAAAAGAAGCTGGAGATGATCGTGCT-3¢ (Strep-tag underlined) and 5¢-GCGGCATGCTCATCCAAAGAAGC TTGGGTCG-3¢ The two amplified fragments...

Ngày tải lên: 07/03/2014, 21:20

... levels, for a period of 60 min, following i.p injection of choline (30, 60 or 90 mgÆkg)1) or saline (1 mLÆkg)1) Serum insulin and glucose levels began to increase within after choline injection, ... of insulin release during the first 10-min incubation period in the absence of added choline The muscarinic receptor binding activity of choline to the pancreatic homogenate was examined using ... 60-min equlibration period,  100 mg of the minced pancreas were transferred into each of six incubation wells Minced pancreas were incubated in mL of Krebs-Ringer buffer containing 3% bovine...

Ngày tải lên: 17/03/2014, 09:20

... primer: 5¢-GATGTTGATGGTGATGGTCA-3¢; reverse primer: 5¢-AAACCAGCCATGAATGAAAT-3¢) and with actin primers (forward primer: 5¢-GTTGGGAT GAACCAGAAGGA-3¢; reverse primer: 5¢-GAACCA CCGATCCAGACACT-3¢) ... used for these experiments are: Oligo I, 5¢-CAAGGACGTTCGATGCA CTTCCAAAAAACATATAAT-3¢; Oligo II, 5¢-CAAT GTAGTATTAAAAAGTAGTAGTTAAAAGC-3¢; Oligo III, 5¢-GTTTTTATCCGATGCAAATTTTTGCTTTGT GATTG-3¢ ... by internal grants from the International Center for Genetic Engineering and Biotechnology and funds from Department of Science and Technology, Government of India We thank Prof S Assmann for...

Ngày tải lên: 18/03/2014, 01:20

... 5¢-AGGGTTAGGGTTAGGGTT-3¢ 5¢-GGGTTAGGGTTAGGGTTA-3¢ 5¢-GGTTAGGGTTAGGGTTAG-3¢ 5¢-GTTAGGGTTAGGGTTAGG-3¢ 5¢-CCTAACCCTAACCCTAAC-3¢ 5¢-GUUAGGGUUAGGGUUAGG-3¢ 5¢-GTTGGGGTTGGGGTTGG-3¢ 5¢-AATTAACCCTCACTAAAGGG-3¢ 5¢-GTAATACGACTCACTATAGGG-3¢ ... 5¢-GTAATACGACTCACTATAGGG-3¢ 5¢- AATCCGTCGAGCAGAGTT-3¢ 5¢-CTGGCCGTCGTTTTACTTAGGGTTAGGGTT AGG-3¢ 5¢- GTAAAACGACGGCCAG-3¢ 5¢-GTACAGTGTACAGTGTACAGT-3¢ 5¢ biotin-GTAATACGACTCGTTAGGGTTAGGGT TAGG-3¢ 0.9, 2.25, ... chromatography Oligonucleotide Sequence Tel1 Tel2 Tel3 Tel4 Tel5 Tel6 Tel6-Rev Tel6RNA Tet-tel T3 T7 TS OvhF OvhR CSB1 5¢ biotinTel6 5¢-TTAGGGTTAGGGTTAGGG-3¢ 5¢-TAGGGTTAGGGTTAGGGT-3¢ 5¢-AGGGTTAGGGTTAGGGTT-3¢...

Ngày tải lên: 23/03/2014, 13:20

... Against HSV-1, it provided about 70% protection, though it was fairly ( 40%) cytotoxic Finally, 0.25 mg/ml in 2.5% ethanol provided about 70% protection against HSV-2 with minor cytotoxic effects ... limit of solubility in water (with 0.85% NaCl) Further studies should focus on examining the compound at lower concentrations (0.5 mg/ml down to 0.05 mg/ml or lower), examining the role of solvent ... concentrations (2.5% ethyl acetate plus virus), examining the effectiveness of a water extract of the plant, or elucidating the mechanism of synergistic cytotoxicity of ethanol and the compound It is possible...

Ngày tải lên: 03/04/2014, 15:20

... Diffusion profiles showing the effect of different grades of Carbopol® polymers on the release of ketoprofen (n = 5) 114 Figure 5.4 Higuchi plots showing the effect of different grades of ... 22 Figure 1.8 Modified Franz diffusion cell 34 Figure 1.9 European Pharmacopoeia diffusion cell 35 Figure 2.1 Figure 2.2 Figure 2.3 Figure 2.4 Figure 2.5 Figure 2.6 Figure 2.7 Figure ... to light at 25°C analysed by uv 80 Figure 4.1 Figure 4.2 Figure 4.3 Figure 5.1 Figure 5.2 Figure 5.3 Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature...

Ngày tải lên: 13/04/2014, 09:25

... time of cell fusion [41] So, it is possible that once differentiation is triggered, the expression of the genetic repertoire of a differentiated tissue in vivo may differ from the observed in vitro ... are being used However, green autofluorescent artifacts were observed in IF muscle analysis after stem cells transplantation in murines [35], calling the attention for the difficulty in the interpretation ... component(s) responsible for the myogenic effects has not been performed, we not exclude the possibility that inflammatory and growth factors with myogenic effect, like IL6/LIF, IGF, HGF, or others [38-40]...

Ngày tải lên: 18/06/2014, 15:20

... GGA GAG GA-3’ for CCL4; 5’TCT GCA GCA CTT CTG TGT CTG-3’ and 5’-GGA TCC TAG AAG GAG CTG GA-3’ for CCL7; 5’-CAG TCC ATG AGA AGG AGT CCA-3’ and 5’-AGA TCC TGC ACA GGA CTG TG-3’ for CCL8; 5’-AGG GCA ... GTG TTG GGG ACA AG-3’ for CXCL9; 5’- CGA TGC CTA AAT CCC AAA TCG AAG CA-3’ and 5’-AAT TGC TGG ACT CCT TTG GGC AGT GG-3’ for CXCL11; 5’-ATG AAC GCC AAG GTC GTG GTC-3’ and 5’-TGG CTG TTG TGC TTA ... ACA TGC AG-3’ for CCL25; 5’-GGC CCT GCC CTT ATA GC-3’ and 5’CTA ACT TGG GGT TGA CAT T-3’ for CXCL1-3; 5’TGT TGA GAG AGC TGC G- 3’ and GGG TTC AGA GAC CTC CA-3’ for CXCL5; 5’-GAA GTG GTA GCC TCC...

Ngày tải lên: 18/06/2014, 19:20

... the effect of stimulating secretion of SDF-1, a powerful mediator of chemotaxis for CXCR-4 expressing killer cells, improving the homing efficiency of chimeric PBLs to bone marrow and enabling artificially ... containing a GFP- chTCR cassette, demonstrating high efficiency transduction with retro-X and a continuous growing cell population, judged here by expression of GFP Direct transfection of PBLs ... 2:449-459 Fitzer-Attas CJ, Schindler DG, Waks T, Eshhar Z: Harnessing Syk family tyrosine kinases as signaling domains for chimeric single chain of the variable domain receptors: optimal design for...

Ngày tải lên: 19/06/2014, 08:20

... aggregates of the fine fraction indicates a combination of natural and anthropogenic sources influenced by smelter and incineration emissions in the study area Table 3: SEM classification of individual ... combustion of fossil fuels and mineral contents; S only was observed in cluster (

Ngày tải lên: 20/06/2014, 00:20