extraction of nucleic acids from bone

... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... the production of PGE2 [18] The inhibition of PGE2 release was the consequence of a reduction of COX-2 and secretory PLA2 mRNA expression Moreover, the IL-1b-induced expression of secretory PLA2 ... cardiomyopathy, probably resulting from hyperactivation of ERK1/2 [32] We postulate that the antiproliferative effect of EPA and DHA is a result of the negative regulation of the MAPK pathway, caused...

Ngày tải lên: 19/02/2014, 16:20

... inhibition of expression of the genes of ÔunwantedÕ proteins The successful selection of hammerhead ribozymes, active in human cell lines, allows application of a molecular approach to the design of ... for the inhibition of the biosynthesis of b-secretase and for the exclusion of the formation of toxic b-amyloid peptides in HEK293 cells Materials and methods Construction of plasmids for ribozyme ... preclearing process of the cell protein extract (100 lg of protein in 500 lL of extract) was carried out as described above Immunoprecipitation and determination of the intracellular level of Ab40 was...

Ngày tải lên: 08/03/2014, 08:20

... depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA (B) Each point is the mean ± SEM of three sets of experiments (C) Real-time PCR quantification of PEPCK and G6Pase mRNA ... have unfolded a series of events beginning with the TNFa-mediated prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis ... absence of any of these were taken as the control On termination of incubation, HepG2 cells were treated as described above with TNFa (1 nm) and ⁄ or insulin (50 nm), or in the absence of any of these...

Ngày tải lên: 16/03/2014, 02:20

... mutant strain of UN4495 (RAS47) carrying a chromosomal nifK¢¢lacZ fusion (A) Synthesis rates of b-galactosidase from the nifHDK promoter were determined from the slope of these plots from at least ... transferred from DMNH2 to the FAD-cofactor of NifL (Fig 4A) The reduction of NifL-bound FAD by a quinol derivative was confirmed using menadiol that also resulted in reduction of the flavin cofactor ... vivo, reduction of purified MBP– NifL was studied in vitro at room temperature In the absence of a redox mediator, the FAD cofactor of oxidized MBP–NifL was not reduced by the addition of NADH (data...

Ngày tải lên: 17/03/2014, 10:20

... Comparison of bone structural and turnover indices between OA and control individuals Bone histomorphometry was performed on IT trabecular bone samples obtained from 14 out of the 15 OA cases from ... or maintenance of bone mass in OA This study of the IT region of the proximal femur in primary hip OA and non-OA postmortem controls investigated changes in gene expression of bone anabolic factors ... is supportive of an increase or maintenance of bone volume in OA individuals versus the agedependent bone loss in the general population [11] ALP is used as an enzymatic marker of bone formation,...

Ngày tải lên: 09/08/2014, 08:23

... cytokine/ chemokine response profile varied with the time-course of infection Our results showed that at the early phase of avian influenza virus infection, the transcription of TNF-a and IL-6 was induced ... and quantitative differences in the profiles of cytokines/chemokines induced by influenza viruses of different pathogenicity H5N1 was a more potent inducer of inflammatory cytokines/chemokines; ... for the inoculation of virus isolate at a multiplicity of infection (m.o.i.) of one After hour of adsorption, the virus was removed and ml of fresh RPMI-1640 media with 2% FBS, 100 U/ml penicillin,...

Ngày tải lên: 12/08/2014, 02:20

... molecules Clickforforerror 2b.variables11fromnotthegenesinprotein clusters Plot of 3Figurefileofto 2genesoffrominFigurenotfunctionally data4file Additionalofand file cluster 3thatfor Figure33 butadditionalrelated ... file 13) The smoothness of the protein profile is even more pronounced in the group of very highly correlated genes (Figure 2b), with an average SD of 0.163 Although 'flat' profiles for mRNA are ... only genes from the MIPS set of protein complexes The analyses for protein profiles showed that they tended to be less unbalanced than those of mRNA, with a highly significant excess of genes with...

Ngày tải lên: 14/08/2014, 08:20

... the technique; the percentage of grains on pig chromosome is increased from 31% to 86% and the region is reduced from (q2.2 - q2.7) to one band q2.5 The number of grains pointed in this region ... out of the 500 grains counted on this chromosome, 330 (66%) were in the region q2.5 (Fig 2) Among the total number of metaphases scored, 87% showed both chromosomes labeled in this region One of ... equaled stein, 1983), H-labeled 10 dpm/pmg Appropriate amount of probe was precipited by adding ethanol in the presence of a 1,000-fold excess of sonicated salmon sperm DNA The precipitate was diluted...

Ngày tải lên: 14/08/2014, 19:23

... lymphomas A number of the genes detected in these experiments have not been previously associated with DLBCL of different origin A comparison of expression profiles of some of these genes with ... (capn4) and other (in case of lymphoma h1) and SMG-1, the gene of ribosomal protein S8 (in case of lymphoma h2), as well as several genes of unknown function (9 and in the case of lymphoma h1 and h2, ... Tanaka A, et al Prediction of the coding sequences of unidentified human genes IV The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1...

Ngày tải lên: 02/11/2012, 11:08

... accentuates the need of further investigations Particularly a better understanding of the influence of the molecular function is necessary, as well as of biochemical processes and of the biophysical ... derived from the DNA sequence of the non-pathogenic Yersina pestis strain 91001 [32] As additional controls, the effects of the HiPerfect Transfection Reagent (Qiagen, Cat No 301704) and of the ... system with a 384-well block and the software version 1.2 The 10µl reaction consisted of 25ng of transcribed RNA (1µl), 1pmol Universal Probe (0.1µl), 50pmol each of forward + reverse primer (0.5µl),...

Ngày tải lên: 03/11/2012, 11:44

... ft from a medium traffic roadway and 1,500 ft south of a lightly trafficked CSX railway One interstate highway is located miles south (I-96) and another miles west (I-496) of the site, both of ... located 1.2 miles northwest of the site The Simon plant emits over 3,000 tons of SO and 1,300 tons of NOx annually In downtown Lansing, approximately 4.5 miles west of the site is a 351MW coal ... carried out at a magnification of ×18500 An average of six to seven visual fields was evaluated for mitochondria analysis The size of mitochondria was analyzed from randomly delineated in five...

Ngày tải lên: 06/03/2014, 19:20

... in the presence of both fatty acids (Table 2) Insulin (1 lm) reversed the effect of the fatty acids (100 lm of each) on PDK mRNA expression by returning transcript abundance of PDK2 and PDK4 ... percentage of basal levels Results are the means ± SEM of n ¼ 3, from cells prepared from three different subjects and values are expressed as a percentage of basal (100%, minus fatty acids and ... effects of glucose ± insulin on PDK2 and PDK4 transcript abundance expressed as a percentage of basal (5 mM) glucose levels Results are the means ± SEM of n ¼ 3, from cells prepared from three...

Ngày tải lên: 07/03/2014, 17:20

... C-terminus (data not shown) The amino acid sequence of a soybean paralog of SEL-1L, which was deduced from the nucleotide sequence of cDNA cloned from young leaves by RT-PCR, was closely similar ... details of the mechanism of plant ERAD, functional characterization of these proteins must be performed In mammalian cells, ER stress responses are composed of three steps, i.e., enhancement of the ... Inc.) Detection of phosphorylated Ser51 of eIF2a Plant tissues cut off from the roots were treated as described above with or without TM for the indicated times Plant proteins (30 lg of proteins)...

Ngày tải lên: 07/03/2014, 21:20

... Plasmids Gene silencing of p53 For production of luciferase reporter constructs, the flanking region of the transcription start site of ANX4, from )1534 to +1010, was amplified from human genomic DNA ... Determination of the 5¢-end of the ANX4 mRNA To determine the 5¢-end of ANX4 mRNA, 5¢-RACE analysis was performed using RNA isolated from MCAS, OVTOKO and OVISE cultured cell lines Single DNA bands of the ... compared to only five of the 62 serous-type samples These findings suggest that the up-regulation of ANX4 is a unique characteristic of ovarian CCA ANX4 belongs to a ubiquitous family of calciumdependent...

Ngày tải lên: 22/03/2014, 16:20

... differences in mRNA levels of four enzymes of the urea cycle Whereas the zonal-specific expression of the main enzymes of drug and xenobiotic metabolism has been the subject of extensive research (e.g ... layers of hepatocytes surrounding the central veins [4] Previous studies on zonation of metabolic pathways in the liver were mainly focused on analysis of protein expression or measurements of enzyme ... hepatocytes from the two different zones of the liver; 99 of these were predominantly expressed in perivenous cells, whereas another 99 were mainly expressed in periportal hepatocytes A detailed list of...

Ngày tải lên: 23/03/2014, 10:20

... B1 isoform) of other insect EcRs and a strong degree of identity was confined to the two ends of N-terminal region of the A/B domain Using a B1 isoform-specific probe from the A/B region of PIEcR, ... transactivating domain [40] The predicted sequence of A/B domain of PIEcR exhibited significant amino acid identities with the corresponding region of B1 isoform of other insect EcRs, despite differences ... ends of the B1 isoform specific segment in the N-terminal region (Fig 4) There was no similarity to the N-terminal specific regions of either the A or the B2 isoform (data not shown) All of these...

Ngày tải lên: 23/03/2014, 13:20

... total of 20 lL reaction volume containing lL of cDNA, lL of each of forward and reverse sequence specific primers (from 10 lm primer stock), 10 lL of supermix (Bio-Rad Laboratories) and lL of nuclease ... water-soluble variant of RSL-1, namely RG-102277 First, we determined the effect of dimethylsulfoxide itself on the expression of genes in 293 cells The expressions of a total of 43 genes were modulated ... interactions of this hypothetical protein are not known RLP is a ribosomal protein which usually decorates the rRNA cores of the subunits Approximately two-thirds of the mass of the ribosome consists of...

Ngày tải lên: 30/03/2014, 03:20

... Department of Oncology, University Hospital of North Norway, Tromso, Norway 2Institute of Clinical Medicine, University of Tromso, Tromso, Norway 3Department of Pathology, University Hospital of North ... al Journal of Translational Medicine 2011, 9:6 http://www.translational-medicine.com/content/9/1/6 Page of by the log rank test DSS was determined from the date of surgery to the time of lung cancer ... 4Institute of Medical Biology, University of Tromso, Tromso, Norway 5Department of Pathology, Nordland Central Hospital, Bodo, Norway Authors’ contributions TD participated in the design of the study,...

Ngày tải lên: 18/06/2014, 16:20

... consisted of μl of diluted cDNA, 0.3 μM of forward and reverse primers (1 μl each), 10 μl of the 2X QuantiTect™ SYBR® green master mix, 0.1 μl of HK-UNG (Epicentre, Madison, WI), and μl of RNase-free ... observed in the HP content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of HP/mg of tissue wet weight Differential ... observed in the GAG content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of GAG/mg of tissue wet weight...

Ngày tải lên: 20/06/2014, 00:20