extraction methods for the gp model parameters

... suitable for converting the parameters of one model to those of another one An example is given for the VBIC model extraction by going through the GP model It is based on the fact that the VBIC model ... Model 90 5.1.3 Empirical Model 91 5.1.4 Behavioral Model 93 5.2 Gummel-Poon (GP) Model 96 5.2.1 Overview of the GP Model 96 5.2.2 Extraction Methods for the GP Model Parameters 98 5.3 Experiments ... large-signal modeling and a detailed review of numerous methods developed for HBT large-signal modeling Methods ranging from the physical model, the semi-physical compact model, the empirical model and the...

Ngày tải lên: 17/09/2015, 17:20

... 3B), these data suggest that these domains are not obligate for the stabilization of the dimers, but rather must contribute to the overall stability of the dimers Crystallographic analysis of the ... species only represented a minor form even in the CD38 p42 monomer Taken together, the data indicate that the p95 form of CD38 appears to represent a homodimeric form of CD38 as it is recognized ... (D) were performed using SIGMAGEL.LNK The CD38 levels for each clone were first normalized to actin by dividing the densitometric value of CD38 by the densitometric value of actin Then the relative...

Ngày tải lên: 23/03/2014, 12:20

... immunolocalization of asporin in the human disc The objectives of the present study, therefore, were to determine the localization patterns of asporin within the human and sand rat intervertebral ... age for the six grade III discs was 45.5 years, and 53 years for the five grade IV discs Asporin immunolocalization The first portion of our study of asporin and the intervertebral disc tested for ... seen in the human disc: the greatest localization was present in the outer annulus, with fewer cells positive in the deepest region of the inner annulus and nucleus pulposus (Figure 4) The presence...

Ngày tải lên: 09/08/2014, 14:20

... CD90 might therefore be a good marker for interstitial tissue fibroblasts, but not for the FLSs forming the epithelium-like lining of the synovium In addition, fibroblasts change the expression ... protein, on the inside of the cell in vitro [30,44] It has therefore been suggested that podoplanin is involved in directing actin polymerisation, thereby forming the cellular protrusions needed for ... contributions AKHE did the major design of the study and the coordination and establishment of the biobank, carried out the experiments, performed the imaging and FACS analysis and drafted the manuscript...

Ngày tải lên: 12/08/2014, 15:22

... for the thermolability of MTHFR Actually, the frequency of the mutated allele was quite high, depending on the ethnic group analyzed [18-21] Some authors suggested that the homozygosity for the ... 20 10 Gp1 Gp2 Groups Gp3 Int J Med Sci 2004 1: 181-192 192 Figure The effect of the T allele on the plasma homocysteine in the different studied groups Bar chart illustrating the effect of the ... transducer The examination was done for the right and left common carotid arteries and the mean values of the two sites were used in the analysis The transducer was positioned over the common...

Ngày tải lên: 03/11/2012, 09:49

... produce the metabolites that they are expected to catalyse and that are found in their plant of origin Therefore, further studies are needed to elucidate the reasons for these failures to reveal the ... responsible for the formation of emodin anthrone The final stages of hypericin biosynthesis are conducted by the gene product of hyp-1, encoding for the phenolic coupling protein that catalyses the oxidative ... important compounds of H perforatum, a widely used herbal remedy for the treatment of depression [1,2], there is little information available about the biosynthesis of these compounds To date, only...

Ngày tải lên: 18/02/2014, 18:20

... 94 °C for 15 s and 68 °C for for 40 cycles For detection of SAF-3 mRNA, nested PCR was performed In the first PCR, the 4284 Northern blot analysis A multiple-tissue Northern blot (Clontech Laboratories ... reaction to further determine whether SAF-3 is indeed initiated from the first ATG codon The SAF-3 protein translated from the predicted ATG codon is 455 amino acids long The next ATG codon in the SAF-3 ... to the ATG start codon of the SAF-3 cDNA The probe and RNA were heated at 90 °C for 15 in 80% formamide, 40 mm Pipes pH 6.4, 400 mm NaCl, mm EDTA buffer, and then incubated overnight at 50 °C The...

Ngày tải lên: 07/03/2014, 02:20

... b-sheet A to form a latent conformer [16] However, this is unlikely as the latent conformer of the serpins is unable to form polymers [17,18] Other characteristics of the latent conformer are a ... CD because the signal at 216 nm did not change during the course of the experiment, i.e after incubation of Ser52Arg neuroserpin at either 37 °C or 45 °C for 24 h Assessment of the conformation ... neuroserpin The most likely cause for the inactivity of Ser52Arg neuroserpin and inaccessibility of the reactive loop is that the mutant had adopted an aberrant conformation One possibility is that the...

Ngày tải lên: 07/03/2014, 16:20

... strategy for amplification of nTG cDNA The horizontal bar indicates the nTG cDNA Thick horizontal bars indicate the sequences of PCR-amplified clones The primers used for PCR are given on the right The ... adequate context for the initiation of translation (Fig 2A); the sequence CCAAAATGG surrounding the ATG fits the consensus sequence CC(G/A)CCATGG for the eukaryotic initiator site [29] The predicted ... phenylmethanesulfonyl fluoride] and the suspension incubated for 30 min, followed by centrifugation for 10 at 17 000 g The supernatant, designated as Sup4, was separated from the pellet For the immunoprecipitation...

Ngày tải lên: 08/03/2014, 10:20

... [11] The dependence of the growth rate on the reactant vapour concentration is not in itself evidence that any of them is the rate-determining step Rather, it simply reflects the dependence of the ... dependent on the type of catalyst This could be explained by the effect of the catalyst on the barrier energy to the nucleation of a new step at the growth front of the nanowires (i.e., the liquid/solid ... thus the growth rate should follow the power law However, this is not the case in the present study as well as in previous studies [11–14] The primary evidence for regarding step (2) as the rate-limiting...

Ngày tải lên: 16/03/2014, 15:05

... at the interface and grow epitaxially on the Si substrate Silicon oxide will flow over the Au particle surface and form a layer sheathing the SiNW The diameter of the SiNWs is thus limited by the ... crystal facets could minimize the total energy of the nanowire because the surface energy of the {1 1} facets is the lowest and the energy of the side surfaces dictates the total surface energy of ... direction, the dominant growth direction of SiNWs was Æ1 2æ with Æ1 0æ being the second dominant direction, the same as the SiNWs grown by the OAG method The Æ1 2æ direction is different from the common...

Ngày tải lên: 16/03/2014, 15:06

... to the manufacturers’ manuals PolyA+ RNAs from various human tissues were purchased from Clontech cDNA synthesis was performed as described above The PCR was performed for 35 cycles of 95 °C for ... Although we did not determine the transcription initiation site, the 5¢ UTR appears to be longer than 67 bp because the primer for the first PCR used to isolate the full-length cDNA was designed ... binding site There is no typical TATA or CAAT box in the putative promoter region The putative signal sequence is encoded by the first exon, and the pro-enzyme fragment is encoded by exons and The catalytic...

Ngày tải lên: 16/03/2014, 23:20

... than 50% of the native enzyme (Fig 1C) The distribution of the KE2 mutant between the two fractions was similar to that of the native enzyme (Fig 1C) In contrast, the activity of the KE2RS2 mutant ... and KM20L2 cells (Fig 4B) The expression of other sPLA2s (IB, IIE and IIF) was undetectable The expression of other enzymes involved in the PGE2biosynthetic pathway in these colon carcinoma cell ... for human sPLA2-IB, IIA, IID, IIE, IIF, V and X (left) and for cPLA2a (right) After staining of the gel with ethidium bromide, samples were taken for Southern blotting using cDNA probes for the...

Ngày tải lên: 18/03/2014, 01:20

... although the Hsp90b expression of PP30[hHsp90b] appeared to be slightly lower than that of the other three strains (Fig 1A) These yeasts, isogenic but for their Hsp90 gene, might therefore be ... phenotypes Nevertheless, as the studies below reveal, the strains expressing human Hsp90a or Hsp90b exhibit some differences These isoforms are therefore not completely identical in their in vivo ... vivo, ATP turnover rate therefore being an important determinant of the length of time for which a client remains Hsp90-bound [39–41] The question therefore arises of whether more inefficient Hsp90b...

Ngày tải lên: 23/03/2014, 07:20

... of GPV in CHO/GPV cells in the absence of GPIb–IX (A) GPV expression on the surface of platelets and CHO/GPV cells was analysed by flow cytometry using the mAb V.1 without permeabilization of the ... processing of GPV in CHO and K562 cells cotransfected with GPV and GPIb-IX (A) CHO cells stably transfected with GPV, GPIba, GPIbß and GPIX were analysed for surface expression of GPV by flow cytometry ... mass form during the 180 of chase On the other hand, an 80 kDa mature form progressively appeared and accumulated in the supernatants at chase times of 60–120 Because at T0 only the 70 kDa form...

Ngày tải lên: 23/03/2014, 13:20

... in multiple species However, the dn/ds ratio is higher for exon 10 (0.40) than for the other exons (0.13) As the ds value is similar for exon 10 (0.62) and the other exons (0.56), this difference ... encoding the NLS and therefore the resulting PCR fragment lacks the NLS sequence This fragment was cloned into pBluescript II SK and the sequence was verified The 200 bp fragment containing the mutated ... fixed for function across mammals However, the dn/ds ratio for exon 10 (0.40) is higher than that for the other exons, which have a dn/ds value (0.13) that is similar to the average of that for...

Ngày tải lên: 23/03/2014, 13:20

... then allowing the red blood cells to settle for 30 The supernatant was harvested and centrifuged for at 500 g The cells were then resuspended in 0.2 Â NaCl/Pi to induce red blood cell lysis for ... digesting the fragment with HindIII and Bgl II and the plasmid with HindIII and Bam HI The fragment was ligated into the plasmid and the integrity of the insertion was validated by digesting the plasmid ... purified (< 820 bp) The insert was then ligated into the pSPORT vector and the resulting full-length siglec-10 clone was designated 995-2 and the sequence was verified against the other siglec-10 clones...

Ngày tải lên: 24/03/2014, 04:21

... Materials and methods The results are expressed as the mean ± SEM percentage of G2 for (A) and (B); as the mean ± SEM arbitrary units relative to actin for (C); and as the mean ± SEM for (D) the low ... 20 for h They were then serially incubated with rabbit anti-PHB serum (1 : 650) for h, MitofluorTM Red 589 for 20 min, anti-rabbit FITC (1 : 650) for h and Hoechst 33342 for The cells were then ... 200 lL dimethyl sulfoxide to dissolve the formazan The cells were left for 30 at room temperature The reduction of MTT to formazan was quantified by measuring the absorbance at 540 and 630 nm using...

Ngày tải lên: 29/03/2014, 08:20

... were diagnosed for the first time during the enrollment period and their blood samples were collected before they received any treatment such as surgery, chemotherapy or radiotherapy This research ... from the greater curvature of the antrum and the midpoint of the greater curvature of the gastric body via endoscopic biopsy In the patients with cancer two specimens were obtained from the cancerous ... carcinogenesis We then estimated the validity of HMGB1 as a potential biomarker for the screening, diagnosis, and surveillance of GC We also analyzed the relationship between serum HMGB1 levels and the clinical...

Ngày tải lên: 18/06/2014, 15:20

... of some of the sperm collection and viability analysis NG participated in the design of the study, and revised the manuscript FG participated in the design of the study, and revised the manuscript ... histological origin For this reason, the definition of the biological role of Sp17 remains an open question The present study was aimed at investigating the localization of Sp17 by morphological methods ... in the cytoplasm of some spermatocytes and that of early and late spermatids [13] The flagella of the spermatozoa in the lumen of the seminiferous tubules was also found to be immunopositive for...

Ngày tải lên: 18/06/2014, 15:20