expression of single domain antibodies in bacterial systems

... diagram of the fusion of cells producing individual protein units of a multisubunit protein Three transfected CHO-K1 cell lines producing either IgA, J-chain or SC, respectively, were fused in two ... The amount of IgA present in each supernatant was calculated relative to a standard preparation with known concentration Verification of J-chain expression To examine production of J-chain, transfected ... were fixed and stained with an anti(human J-chain) Ig to verify the presence of intracellular J-chain A further attempt to directly quantify the amount of intracellular J-chain was avoided, as...

Ngày tải lên: 18/03/2014, 01:20

... light chains occur naturally in the Camelidae [1], several groups have reported the isolation of singledomain antibodies (sdAbs) consisting of the variable domain of these heavy chain antibodies, ... of two bands corresponding two the VH + CH1 + hinge + part of CH2 gene fragment of traditional antibodies CEA-specific single domain antibodies or the VHH + hinge + part of CH2 gene fragment of ... shown in Fig 5, the presence of a large excess (more than two orders of magnitude) of untagged sdAb C17 did not interfere with the binding of gold mAbs, but completely inhibited the binding of c-myc-tagged...

Ngày tải lên: 23/03/2014, 05:22

... through binding of two LXXLL motifs in the activation domain of the viral protein to the KIX domain of the coactivators [47] Mutating both LXXLL motifs, which severely diminishes binding to p300/CBP, ... Activation of Dkk1 expression requires the LXXLL motifs in the activation domain of HBZ The N-terminal activation domain encompassing the LXXLL motifs and the C-terminal bZIP domain in HBZ target ... Dkk1 expression HBZ activates DKK1 gene transcription in an uninfected and an HTLV-1-infected T-cell line We were interested in determining whether the expression of HBZ alone induces DKK1 gene expression...

Ngày tải lên: 13/08/2014, 01:20

... protein molecules and their interactions with DNA in real-time reactions and the dynamics of their DNA-binding Therefore, my objective of finding the characteristics of protein binding to single ... the expression of diverse virulence genes The Nterminus of SsrB contains the site of phosphorylation and the C-terminus consists of a DNA-binding domain Dimerization occurs upon DNA binding and ... terminal region of MDP1 shows it contains the dimerization domain and DNA binding domain (Figure 1.16) (Bhowmick et al, to be published) Figure 1.16 Dimerization formation of MDP1 N terminal domain...

Ngày tải lên: 09/09/2015, 11:25

... N-terminal domain for protein oligomerization, C-terminal domain for DNA binding, and flexible linker connecting the two domains [48, 51] The central region of H-NS, which includes the flexible linker, ... to probe the DNA binding properties of these important proteins at the singlemolecule level Single- molecule manipulation using magnetic tweezers and single- molecule imaging using atomic force ... force-extension curves of naked DNA as our control experiment By changing the bu↵er to those containing DNA-binding protein of our interest, we can study the DNA binding properties of the protein by its impact...

Ngày tải lên: 09/09/2015, 11:26

... may in uence the expression of HO-1 The dominant expression of HO-2 protein, in comparison with the low expression of HO-1 protein, in H146 small cell lung cancer cells is of particular interest ... the expression of HO-2 mRNA and protein and induced the expression of HO-1 mRNA and protein In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins ... determine the basal expression levels of HO-1 Effects of HO-1 or HO-2 short interfering RNA (siRNA) on the expression of HO-1 Fig Effects of SA and SnPP on cellular heme content in human cell lines...

Ngày tải lên: 19/02/2014, 05:20

... HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of ... regions including a large intron of the HO-2 gene are required In summary, the present study has suggested that the reduced expression of HO-2 protein may contribute to the maintenance of intracellular ... expression in human cells under hypoxia In the present study, we have analyzed the effect of hypoxia on the expression levels of HO-1 and HO-2 in various types of human cell line, including erythroleukemia...

Ngày tải lên: 19/02/2014, 06:20

... distinctive structural features of algal [Fe]hydrogenases are also present in the C reinhardtii HydA2 amino-acid sequence [33–35], including the well-conserved C-terminal part (C -domain) that binds ... differences in the regulation of expression of the two genes Gene expression during anaerobic induction The coexpression of the HydA1 and HydA2 transcripts was studied by Northern blot analysis In Fig ... usually rich in serine, threonine, alanine and valine, but are deficient in acidic residues [36] Moreover, chloroplast transit peptides have three characteristic domains [37] that are present in the...

Ngày tải lên: 20/02/2014, 11:20

... granules in skin melanophores resulting in darkening of the skin [23] POMC is the major cargo protein in this cell type and during adaptation to a black background the amount of POMC mRNA is induced ... population of neuroendocrine cells, but also of PrPC transgene expression, providing an additional tool for studying PrPC function Fig Schematic of the biosynthesis and processing of GFP–PrPC in intermediate ... (University of Leuven, Belgium [56]), and against the protein-folding chaperones calnexin and binding protein BiP by K Geering (University of Lausanne, Switzerland [57]) Monoclonal anti-tubulin IgG...

Ngày tải lên: 16/03/2014, 14:20

... shown in the inset in (B) and in inset in (D) These results are in agreement with recent findings of Thiboutot et al [36] of 22R-hydroxycholesterol conversion into 17-hydroxypregnenolone in cultured ... occurring in 7-DHC, could affect 7-DHP hydroxy derivatives generating vitamin D3-like compounds (Fig 8) Such putative conversion would explain the lack of increase in vitamin D3 concentrations in ... chloroform/methanol (1 : 1, v/v), and the associated radioactivity measured by scintillation counting Fig Expression of MLN64 protein (arrow) in human skin (left) and human and rodent skin cells...

Ngày tải lên: 23/03/2014, 13:20

... shows that the UCP3 signal of human recombinant protein interacting with the antibody to human UCP3 increases linearly as a function of increasing amounts of the protein over the relatively large ... from the number of experiments in parentheses SS Fig Increase in the UCP3 signal as a function of increasing amounts of (A) human recombinant protein and (B) mouse recombinant protein Representative ... amounts of UCP3 protein and mRNA in the three muscles are comparable Effect of fasting on UCP3 protein expression We and others have shown that fasting induces upregulation of UCP3 mRNA expression in...

Ngày tải lên: 24/03/2014, 04:21

... displaying two putative splice variants [Fig 2] Expression of fucokinase cDNAs The two splice variants of fucokinase were subcloned into the XbaI site of a pQM vector containing a C-terminal E2-Tag/A ... variants of L-fucokinase The long splice variant of L-fucokinase consisted of 3270 bp, encoding a protein of 1090 amino acids The sequence of the shorter cDNA was similar to the sequence of the IMAGE ... alternative splicing maintains the reading frame along the entire protein, therefore the protein variants are identical in the amino-terminal end up to the alternative splice area, in addition to...

Ngày tải lên: 30/03/2014, 13:20

... constitute the basis of my criteria for a successful hypothesis COMBINING User modeling factors affect the expression of instructions, including the way clauses are combined In the following examples ... 5678, Portland, OR 97228 Cumming, Susanna (1991) Nominalization in English and the organization of grammars In Proceedings of the IJCAI-91 Workshop on Decision Making Throughout the Generation ... general skills involved in using a standard telephone, of which making a call is one Hovy, Eduard H (1989) Approaches to the planning of coherent text Technical Report ISI]RR-89-245, USC Information...

Ngày tải lên: 31/03/2014, 06:20

... interaction of this plant with S meliloti [9] Our previous results suggested that MsPG3 might be involved in the early stages of the interaction, including infection thread formation and reinitiation of ... observed in the cell border and the perinuclear region, containing higher density ER The intensity of the latter fluorescence is weak (H) Root hair in growth terminating stage Fluorescence is mainly ... Preferential localization of the fluorescence was observed in the region of the emerging root hairs (ERH), both in a hair in the bulge stage (B) and in a growing root hair in the phase of organelle zonation...

Ngày tải lên: 31/03/2014, 07:20

... strains β-actin was used as an internal control of a housekeeping protein Note the increasing strains result in an increase of type X collagen protein level while the level of β-actin remains ... metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs), and an increases of the expression of proinflammatory cytokines IL-1β and TNF-α [14-16] Thus, differential gene expression ... weights of the immunoreactive proteins were determined against two different sets of protein marker ladders Quantification of strain distribution across the sponge Strain distribution was determined...

Ngày tải lên: 20/06/2014, 00:20

... for correct folding and intracellular transport of viral glyco- proteins, as shown for instances for influenza hemagglutinin [19] In order to investigate the importance of the individual N-glycosylation ... transiently in Vero cells using the beta-actin promoterdriven mammalian expression vector pCAGGS Western blotting was performed using antiserum directed against the C-terminus of GP-2 As shown in Fig ... incubated twice for 15 at 4°C with mg/ml sulfo-Nhydroxysuccinimidobiotin (Pierce) by adding ml of the biotinylating reagent After biotinylation, cells were washed twice with cold PBS containing...

Ngày tải lên: 20/06/2014, 01:20

... Gaussian noise In Figure 1, the first ring of interference is denoted by shaded cells The problem we tackle is that of finding the antenna spacings in vector Δ = [Δ12 Δ23 ]T (as shown in the example) ... design in interferencelimited scenario, the interference power minimization is analytically tractable and highlights the justification for unequal spacings Therefore, the problem of minimizing the interference ... antenna spacings Δ through nonlinear relationships The first problem we tackle is that of finding the set of optimal spacings Δ that minimizes the average (with respect to fading) interference...

Ngày tải lên: 22/06/2014, 19:20

... “Simplifying the integration of processing elements in computing systems using a programmable controller,” in Proceedings of 13th Annual IEEE Symposium on Field-Programmable Custom Computing Machines ... Leeser, “Precision modeling of floating-point applications for variable bitwidth computing,” in Proceedings of the International Conference on Engineering of Reconfigurable Systems and Algorithms ... roles of FPGAs in reprogrammable systems, ” Proceedings of the IEEE, vol 86, no 4, pp 615–638, 1998 [20] R Hartenstein, “Trends in reconfigurable logic and reconfigurable computing,” in Proceedings of...

Ngày tải lên: 22/06/2014, 22:20

... rh 84 rof SBP ni esorcus %03 ni detcetorpoyrc neht dna C ta thginrevo evitaxif emas siht ni dexif-tsop erew seussiT desu erew ,2.7 Hp ,SBP M 1.0 ni edyhedlamrofarap %4 evitaxif yb dewollof ,)2.7Hp ... epotipe na sezingocer dna seidobitna lanolcylop deifirup ytiniffa si ydobitna yramirp sihT )ASU ,zurC atnaS( 001 : detulid ,nilubolgonummi AkrT-itna tibbar htiw C ta rh 84-42 rof detabucni erew ... ,rotceV( seidobitna tibbar itna yradnoces detalynitoib htiw C ta thginrevo detabucni dna ,)spets gniwollof eht fo hcae neewteb demrofrep saw erudecorp hsaw emas( TR ta hcae nim 01 ,SBP ni semit dehsaw...

Ngày tải lên: 07/08/2014, 18:21

... gniniatnoc lonahtem %001 ni nim 03 rof desremmi erew snoitces eussit revil eht ,noitardyh dna noitaziniffaraped retfA desu saw dohtem )CBA( xelpmoc nitoib nidiva eht ,RxrT fo gniniatsonummi roF ... fo yrtsimehcotsihonummI ylevitcepser ,RxrT eht rof yrtsimehcotsihonummi rof dna noitanimaxe lacigolotsih rof gniniats )E&H( nisoe dna nilyxotameh rof deraperp erew snoitces eussit mµ nehT niffarap ... ,4002 edixO cirtiN sisenegonicrac detaidem-noitammalfni fo ledom a : htiw detcefni sretsmah ni egamad AND evitartin inirreviv sihcrohtsipO dna evitadixo detaidem-ON fo msinahceM S ihsinawaK ,P nrowahtihtiS...

Ngày tải lên: 07/08/2014, 18:21