... December2009)doi:10.1111/j.1742-4658.2010.07559.x The extracellular phytase ofthe plant-associated Klebsiella sp. ASR1 is a member ofthe histidine-acid-phosphatase family and acts primarily as a scavenger of phosphate groups locked in the ... substrate-freeAppA the C a atoms are 2.41 A ˚apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚.Distinct conformational changes were observed ... con-served active-site motifs, RHGXRXP and HD, andhydrolyze metal-free phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ bdomain and a small a domain with the catalytic...
... quantitative and qualitative assays for b-galactosidase (activation of LacZ reporter gene). BD, pGBT9; BD*, pAS2-1; AD, pGAD424;AD**, pACT2. Activity values are given as mean values ± standard ... PCR-amplified from the DNA ofthe cosmid clone #9 [18] using the following pair of primers: 5Â-GACTGCAGTGAAGGGCATCGAGTCCTCGGG-3Â and 5Â-GAGGATCCGGGACATTCCTTAGCCAGGAGGG-3Â.Tomaketheb-galactosidase expressing ... the cDNA #911using the following pair of primers: 5Â-CTGGATCCCTATGTCGTGTCCCAGGAGCATCGAG-3Â and 5Â-GTCTGCAGTTAAAAATTCGGGACATTCCTTAGCCAGG-3Â. BamHIPstI digested PCR product was cloned as a fusion...
... glycosyl-transferase elucidate catalysis in the alpha-amylase family. Nat.Struct. Biol. 6, 432–436.16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai, A. , Henrissat, B. & Dijkstra, ... However, the mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues in the sameenzyme.To obtain an insight ... regard to theroleof Asp140,whichappearstobecrucialinChiBandinchitinaseA1from Bacillus circulans, whereas it may be mutated toasparagine without loss of activity in other family 18chitinases...
... The Marketing Strategy ofa multinational join stock companymultinational join stock company has a certain advantage: a multinational join stock company has always kept its prices as competitive ... department is in charge of marketing plans on importing, material providing and preceding the contracts. Organizing thesalesof products is another main task ofthe department.ã Financial and ... stock company size and market share have been small. The shortage of capital is main cause ofthe company’s modest size and market share. Up to now, a multinational join stock company has had only...
... in the child DataTable. This is the default. None Indicates that no action takes place. SetDefault Indicates that the DataColumn values in the child DataTable are to be set to the value in the ... you change the DataColumn in the parent DataTable on which the ForeignKeyConstraint was created, then the same change is also made in any corresponding DataRow objects in the child DataTable. ... UpdateRule is set to None. ã The CommandText ofthe Command object in the UpdateCommand ofthe DataAdapter is the same as in the second case. The following code sets the UpdateRule of the...
... that chimeric AK,BTT, which is composed ofthe catalytic domain of BsAKII and theregulatory domain and b-subunit of TtAK, had thermostability as high as that of wild-typeTtAK, suggesting that ... differential scanning calorimetry; EcAKIII, aspartate kinase IIIfrom Escherichia coli; MAD, multiwavelength anomalous diffraction; MjAK, aspartate kinase from Methanococcus jannaschii; TtAK, aspartatekinase ... catalyticdomain from BsAKII and regulatory domains (a regulatory domain in the a- subunit, and a b-subunitwith the same sequence as theregulatory domain)from TtAK, improved thermostability as much aswild-type...
... oAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus119119119119119120119121118119159159159159157158157160158159199199199199197198197200198199VI ... (Fig. 1A) , and that of chloroplast spinach GAPDH [35] w ere examined t oAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus119119119119119120119121118119159159159159157158157160158159199199199199197198197200198199VI ... R A R AAA L N I V P T S T G AA K A VAL V L PNL K G K L N G I A L R V PR R A R AAA L N I V P T S T G AA K A VAL V L PTL K G K L N G I A L R V PR R A R A ACL N I V P T S T G AA K A VAL...
... link the senses acrossdictionaries, hence Wik is only used as augmenteddata for WMF to better learn the semantics of words.All data is tokenized, POS tagged (Toutanova et al.,2003) and lemmatized, ... this case, many senses will share the same latent semantics profile, as long as they are in the same topic/domain.To solve the sparsity issue we use missing wordsas negative evidence of latent ... (adjectives and adverbs) do not have a taxonomic representation structure. For example, the jcn similarity measure (Jiang and Conrath, 1997)computes the sense pair similarity score based on the information...
... Peplow, F. Yamakura and T. Matsumoto for the analyses of iron and manganese in protein samples. We also wish tothank H. Steinman for the gift of E. coli OX32 6A. We finally thank MrM. Farrugia for ... helper phage (Stratagene). One microgram wasutilized in mutagenesis reactions together with oligonucleo-tides ECF-Q69G d(5Â-AACAACGCAGCTGGGCTCTGGAACCAT), ECF -A1 41Q d(5Â-TCAACCTCTAACCAGGCTACTCCGCTG) ... d(5Â-AACAACGCTGGCCAGCACGCTAACCAC) and ECM-Q14 6A d(5Â-TCTACTGCTAACGCGGATTCTCCGCTG) following the manufacturers instructions (mutagenic nucleotide areunderlined). Mutated plasmids were designated...
... ideas today.Through all the technical discussions, all that technical FCC andPUCA rigmarole, if we can just all agree that in a broadband worldit is all the same and Americans ought to have access ... regulatory treatment of cable andwire-line broadband services are around the corner. The FCC hasalready ruled that the cable broadband falls under Title I ratherthan Title II.Absent another ... and theregulatory approach is fundamen-tally grounded in a wireline paradigm. In the regulated market, for example, LATAboundaries matter. In the unregulated market, they do not. The regulated...
... structure of an HHMM The models discussed here are evaluatedby applying them to natural language tasksbased on CoNLL-20041and a sub-corpus of the Lancaster Treebank2.Keywords: information extraction, ... Grammar ParsingCreation ofa parse tree involves describing lan-guage grammar in a tree representation, whereeach path ofthe tree represents a grammar rule.Consider a sentence from the Lancaster ... 6: The graph of micro-average F -measureagainst the number of training sentences duringtext chunking (A: MHHMM, B: HHMM and C:HMM) The first finding is that the size of training datadramatically...
... and barley a- amylases are thus also presented.MATERIALS AND METHODSSUMAsoftware: subsite mapping of amylasesThis software calculates the apparent binding energies on the basis ofthe measured ... PPA, an a- amylase studied by us earlier. Also an attempt has beenmade to use this program for subsite mapping of other a- amylases found in the literature. Evaluations of subsitemaps of rice and ... Mapping of a- Amylases) is freely availablefor research and educational purposes via the Internet(E-mail: gyemant@tigris.klte.hu). The advantages of this program are demonstratedthrough a- amylases...
... Murakawa, M., Takahashi, S., Tsubuki, S.,Kawashima, S., Sakamaki, K. & Yonehara, S. (1998) Purification,molecular cloning, and characterization of TRP32, a novelthioredoxin-related mammalian ... hTRXL-N may accountfor the formation ofa monomer, instead ofa dimer in the case of TRX. Furthermore, the loss of intermoleculardisulfide-bonds and the disbandment ofthe hydrophobicpatch may also ... C-terminal regionis rich in acidic amino acids, if it does have some interactionwith the N-terminal domain, the mechanism of regulating the catalytic activity may be similar to that ofthe dimer....
... the reaction mechanism of a bacterial ATP-citrate lyaseTadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi and Tadayuki ImanakaDepartment of Synthetic Chemistry and Biological Chemistry, Graduate ... products,AclA (a subunit) and AclB (b subunit). By comparing the primary structures of AclA and AclB with that of the mammalian enzyme, we found that AclA and AclBCorrespondence to T. Imanaka, Department ... reaction can be assumed to be the nucleophilic attack of CoA to the phosphorylated carbonyl carbon of citrylphosphate, and the cleavage ofthe resulting citryl-CoA toacetyl-CoA and oxaloacetate....