experience from in vitro and in vivo studies

... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... thylakoid membrane with a stromal loop and its N- and C-termini facing the lumen PSI-G is markedly protected from trypsin degradation, both in vitro and in vivo, which means that the positively...

Ngày tải lên: 07/03/2014, 21:20

... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... progesterone and androstenedione in bovine theca cells in vitro[ 38] The results from Experiment clearly indicate that treatment of follicles in vivo with inhibiters of the Akt and Erk pathways in the ... number and levels of Akt and Erk in granulosa cells in vitro Cells treated with FSH or IGF alone showed an increase (P < 0.0001) in the secretion of inhibin-A, activin-A, follistatin and oestradiol,...

Ngày tải lên: 20/06/2014, 07:20

... immunomodulating properties in in vivo mouse model MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt Lactobacillus strains isolated from feces of healthy infants ... Serum and Vaccine Producer Biomed, Cracow, Poland), Salmonella enterica subsp enterica sv Enteritidis (clinical isolate obtained from the Institute of Sea and Tropical Medicine, Gdynia, Poland), ... 2000) Experimental design in vivo Eight-week-old inbred BALB/c mice (n = per group) were used for in vivo experiments Strains selected in in vitro tests were mixed in equal proportions, lyophilized...

Ngày tải lên: 07/07/2014, 19:20

... ligand-receptor interaction and a cysteine rich region The intracellular domain consists of ankyrin repeats, a glutamine-rich domain and a PEST (proline, glutamate, serine, threonine) domain ... recent studies have focused on the involvement of this pathway in joint pathology Several studies were interested in the interaction between Notch signaling and cartilage subpopulations [68,69] In ... differentiation and maturation continue into early stages of development, recent and current studies are more interested in the expression of Notch in post-birth and mature articular cartilage Indeed,...

Ngày tải lên: 12/08/2014, 15:22

... reduction in glucose and insulin levels 15mins after oral glucose load in an OGTT [111] However, no effect was observed on fasting glucose and insulin levels, contradicting the previous finding in normaglycemic ... Activation of insulin signaling upon binding of insulin to insulin receptors causes suppression of gluconeogenesis in liver and kidney [14], translocation of glucose transporter-4 (GLUT 4) from inner ... caffeine, and (iv) CGA possesses hypoglycemic effects in human, animal and in vitro models, the present author's next aim is to investigate the hypoglycemic effects of CGA in both in vivo and in vitro...

Ngày tải lên: 08/09/2015, 18:22

... liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in ... mgÆkg)1 body weight, intraperitoneally) Blood was collected from the aorta in heparinized syringes and then liver and lung samples were taken and processed immediately Retinol and retinyl palmitate ... [27] In rats Fig Western blot analysis of p53 and c-Jun in liver and lung Total protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in...

Ngày tải lên: 08/03/2014, 08:20

... were obtained by venipuncture and separated in aliquots One of them was collected in tubes containing EDTA and assigned to determine haematimetric indexes, plasmatic protein concentration and rheological ... Consequently, its increase may be explained by the rise in fibrinogen and/ or globulin concentration and/ or to the modification of the erythrocyte surface properties In our in vitro experiments, ... rheological and flow properties Considering that HA is increased in inflammatory processes and other malignancies, and that it is employed in pharmacologic and cosmetic treatments, in all these...

Ngày tải lên: 10/08/2014, 05:21

... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and O-chain polysaccharide were produced during the 72-h in vivo culture period In addition, sugars detected in the inoculum ... 271) strain 80204-1 produced under in vitro growth conditions on tryptic soy agar (TSA) and in vivo and have demonstrated that their structures are chemically and antigenically distinct from the...

Ngày tải lên: 23/03/2014, 13:20

... obtained from Sigma Chemical (USA) Cyclophosphamide was obtained from Hengrui Pharmaceutical (China) Cell lines and cell culture Malignant cell lines in this study include human lung adenocarcinoma ... shikonin-like compounds Two compounds, namely acetylshikonin and isobutyrylshikonin, have been isolated from the culture vegetal cell suspension This study aims to evaluate the in vivo and in vitro ... acetylshikonin (I) and shikonin (II) Chemical structures of acetylshikonin (I) and shikonin (II) cells Natural shikonin-like compounds also have significant in vivo antitumor effects In a study...

Ngày tải lên: 13/08/2014, 15:21

... samples from the twenty patients with significant response to the treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... inflammation following SIT therapy In conclusion, our in vivo and in vitro studies presented the evidence that not only Th2 but also Th17 mediated inflammation was involved in the AR pathological...

Ngày tải lên: 23/09/2015, 14:47

... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and...

Ngày tải lên: 18/02/2014, 08:20

... USA) and transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein ... tag were obtained by in vitro translation in wheatgerm extract (Invitrotec) according to the manufacturer’s procedure OsRPA70a (or OsRPA70b) and OsRPA32, or OsRPA32 and OsRPA14 were incubated with ... soluble in the presence of m urea Soluble fractions were loaded on to columns containing 10 mL His-Bind resin (Novagen) The columns were washed with 50 mL binding buffer containing m urea and then...

Ngày tải lên: 07/03/2014, 21:20

... protein, calculated as (I ) Imin) ⁄ (Imax ) Imin); I is the fluorescence intensity at a given temperature, and Imax and Imin are the maximum and minimum values of fluorescence intensity corresponding ... Crescenzi O, Tancredi T & Picone D (2003) Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase Eur J Biochem 270, 4729–4735 Leich F, ... protein was calculated as (Q ) Qmin) ⁄ (Qmax ) Qmin); Q is the ellipticity at 222 nm at a given temperature, and Qmax and Qmin are the maximum and minimum values of ellipticity corresponding to...

Ngày tải lên: 22/03/2014, 17:20

... education and training to inculcate the universal competencies and to track simply whether individuals have (1) experienced different elements of that education and training and (2) participated in ... throughout the survey and in one final open-ended question The actual - survey instrument, including instructions and DAL competency definitions, is provided in the appendix - - Survey Administration ... competency or skill They then indicated the single “best” learning environment for the skill or competency in question Settings included initial training, on-the-job training (OJT)/normal duty assignments,...

Ngày tải lên: 23/03/2014, 01:20

... protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes from ... harvest by adding SDS sample buffer and protease inhibitor cocktail, and boiling for 10 to protect proteins from nonspecific proteolysis S pneumoniae cells maintain the full length SPase I in the highest ... results intrigued us to investigate the cleavage of S pneumoniae SPase I in vivo As shown in Fig 6C, Western blot analysis demostrated that the self-cleavage of SPase I is indeed occuring in vivo in...

Ngày tải lên: 23/03/2014, 21:21

... Dioxin from Pulp and Paper and Lead in Soil at Mining Sites i Abstract This paper discusses EPA’s acquisition and use of science in addressing dioxins (and other organochlorines) from the pulp and ... coordinating role and concentrated on the risks from effluents, sludge, and occupational exposures The EPA coordinator was a Dioxin from Pulp and Paper and Lead in Soil at Mining Sites 25 Dwain Winters, ... Dioxin from Pulp and Paper and Lead in Soil at Mining Sites A Control of Dioxins (and Other Organochlorines) from the Pulp and Paper Industry under the Clean Water Act Background The term dioxin...

Ngày tải lên: 24/03/2014, 05:20

... signal of binding of the fluorescent probe is given as extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent binding from total binding for each cell line BiB10-BSA ... avoided In the solid-phase assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding ... neoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice after injection of radioiodinated material Binding to cells and organs In these...

Ngày tải lên: 30/03/2014, 13:20

... evaluated in cell culture and in intranasally infected mice The deletion of F2L had a minimal impact on viral replication in vitro and did not appear to significantly reduce the virulence in vivo ... repeatable increase in the efficacy of N-methanocarbathymidine (N-MCT) (Table 2) This compound is a carbocyclic thymidine analog that inhibits the replication of VV both in vitro and in vivo [12,13], and ... differences in replication in mice reflect real differences in the biology of these viruses, or are related to differences in the animal models including route of infection In HSV, direct intracranial inoculation...

Ngày tải lên: 20/06/2014, 01:20

... RNA from infected cells that were not treated with SCH 16 showed the presence of JEV proteins (Figure 4) In vivo evaluation of compounds against JEV using mouse model After ascertaining the in vivo ... reports describing the in vitro inhibitory effect of compounds such as ribavirin, mycophenolic acid, imino sugars, inhibitors of serine protease, RNA interference and non-steroidal anti-inflammatory ... translation using an in vitro translation kit Western blot illustrating the effect of SCH 16 on JEV translation using an in vitro translation kit Lane 1-uninfected cell control, lanes and – in vitro...

Ngày tải lên: 20/06/2014, 01:20

... J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis Nat Med 2003, 9:347-351 Gitlin L, Karelsky S, Andino R: Short interfering ... growing in a monolayer, and having a well-defined tendency for parallel orientation Viral infection causes a marked CPE resulting in total cellular detachment, rounding, and destruction, which ... 36 and 48 h postinfection Samples of supernatant were obtained at designated time points, and the TCID50 were determined by the Reed-Muench formula BHK-21 cells are fibroblastic, growing in a...

Ngày tải lên: 20/06/2014, 01:20