estimation shape invariant modelling and structural analysis

... Da and an isoelectric point of 4.81 A search in GenBank revealed 86% and 67% sequence identity between the C sepium b-amylase and the b-amylase from sweet potato and soybean, respectively Analysis ... surfaces was 1.4 A and a standard ˚ Stern layer was used to exclude ions from the 2.0 A molecular surface [37] The inner and outer dielectric constants applied to the protein and the solvent were, ... Concentrations of glucose, maltose and cyclohexaamylose were 80 mM , 80 mM and 1.25 mM , respectively The substrate concentration ranged between 0.025 and 0.5% (w/v) Fig SDS/PAGE and isoelectric focusing...

Ngày tải lên: 22/02/2014, 07:20

... Rotterdam, the Netherlands Organization for Health Research and Development (ZonMw), the Netherlands Organisation for Scientific Research (NWO), the Ministry of Health, Welfare and Sport, and the Ministry ... September 2010] Beelen R, Voogt M, Duyzer J, Zandveld P, Hoek G: Comparison of the performances of land use regression modelling and dispersion modelling in estimating small-scale variations ... month, week (e.g., working days and weekend days), and day (e.g., morning and evening rush hour) The adjustment for temporal patterns was performed for traffic and for household emissions Traffic...

Ngày tải lên: 06/03/2014, 19:20

... allergic to BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic ... glycopeptide with a signal at m ⁄ z 1608.7 obtained by PSD analysis amidase-F and glycoamidase-A were permethylated and subjected to MALDI MS and MS ⁄ MS analyses, taking advantage of the enzyme’s ... a-(1,3)-fucosylated, and a small portion also carries core xylosylation and ⁄ or additional GlcNAc This pattern of heterogeneity was demonstrated by MS analysis of both the glycopeptides and the released...

Ngày tải lên: 07/03/2014, 12:20

... DNA polymerase (Toyobo, Japan), using the bF and 68r primers, bR and 68f primers, aF and 109r primers, aR and 109f primers, aF and 114r primers, or aR and 114f primers Following an initial denaturation ... 55 °C, and a incubation at 68 °C The second PCR was also performed with KOD-plus DNA polymerase, using the two megaprimers and the bF and bR primers, or the two megaprimers and the aF and aR ... reaction, was used The 0.72 kb DNA fragment and pUC18-NHase were digested with EcoRI and NotI, and the 1.08 kb DNA fragment and pUC18-NHase were digested with NotI and HindIII The PCR product was ligated...

Ngày tải lên: 16/03/2014, 16:20

... Protein Data Bank IDs 2D8D, 1YBZ and 1ECM 2D8D and 1YBZ are annotated as CMs from Thermus thermophilus (TtCM) and Pyrococcus furiosus (PfCM), respectively, from Structural Genomics projects on ... structural and sequence homology with EcCM, the active site residues of 90-MtCM can be identified, and are shown in Fig The striking difference between EcCM and 90MtCM is that the EcCM residues Ser84 and ... (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such as Bacillus subtilis (BsCM) [18]’ was corrected to ‘Erwinia herbicola CM (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such...

Ngày tải lên: 17/03/2014, 17:20

... incandescent lamps (OSRAM, Germany) Seeds and seedlings were watered six days a week and given a complete nutrient solution once a week Plant tissue preparation and examination For structural analysis, ... provide valuable insights into elucidating and understanding the biochemical and structural basis of the abscission process [26] Such mutants have been described and characterized in a wide range of ... harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing the experiments, structural analysis and the drafting of the manuscript MA...

Ngày tải lên: 12/08/2014, 03:20

... tables and graphics in these pages summarize all known modifications from different experiments, along with relevant literature and information about the number and type of sequence and structural ... source The database is publicly available online, and experimentalists are encouraged to submit their data for storage and display Results Handling and storage of phosphosite data The mtcPTM database ... structures (see Materials and methods, below) The NR set comprised 324 structural models, representing a wide range of Pfam domains, and contained 264 modified serine/threonine and 219 tyrosine residues...

Ngày tải lên: 14/08/2014, 07:21

... SCF complex, and forms the structural link between Cul1 and substrate recognition proteins [85-87] Skp1 proteins are only distantly related to other BTB families (Figures and 6), and are composed ... of the fold, and appear to have two leading β-strands in a region preceding the core fold, with an additional β-strand between A1 and A2 (Additional data file 1) deposited research Structurally, ... [116], CE [117] and VAST [118] structure superposition searches Structural superpositions and sequence alignments were generated with CE, SwissPDBViewer [119] and by manual inspection and adjustments...

Ngày tải lên: 14/08/2014, 14:22

... albicans and fission yeast Schizosaccharomyces pombe, plants such as Arabidopsis thaliana, and metazoans such as Drosophila melanogaster and Homo sapiens, are longer and more complex and exhibit ... with 46%, 87% and 33% similarity and hsChl4R shares conserved blocks of 20 and 50 amino acids with 45% and 40% similarity (Figure 5) The potential human orthologs of S pombe Fta1 and Sim4 were ... a systematic analysis of sequence relationships among a set of approximately 50 fungal, plant and metazoan kinetochore proteins with the overall aim of exploring their structural and evolutionary...

Ngày tải lên: 14/08/2014, 16:21

... proceed the structural analysis, in order to identifl potentially critical regions In order to reduce the computational cost of the analysis and to minimize the input data, the structural analysis ... components analysis techniques in the aerospace industry in a first moment and, more recently, in the automotive industry Among them are the analysis methods like the finite element method and time ... methodology can be easily extended to other structural elements ABSTRACT The present paper shows some of the results obtained for service simulation and structural analysis of a small size vehicle with...

Ngày tải lên: 16/09/2016, 17:13

... address the static analysis and displacement analysis resulting First two (2-D) dimensional brackets and Then 3-D Model of Column Bracket are created for further analysis The purpose of analysis is ... conditions In the analysis Solid Works and COSMOS software is used and the analysis is carried out in two loading steps 133 International Journal of Mechanical Engineering and Technology (IJMET), ... OD 250 mm, ID 200 mm Material Structural Steel Mass 190 kg The Static stress analysis is applied to calculation, which address the static analysis and displacement analysis resulting Figure.6:...

Ngày tải lên: 07/10/2016, 00:03

... demand and pollution According to the concepts of indirect water consumption and wastewater discharge, the two linkages can indicate a sector's economic pull and push forces on water demand and ... (Richardson, 1972; Mayer and Pleeter, 1975; Round, 1983; Miller and Blair, 1985; Brand, 1997; Flegg et al., 1995; Flegg and Webber, 1997, 2000; Hubacek and Sun, 2005; McCann and Dewhurst, 1997; Yu ... economic development, food security and poverty reduction On the other hand, the rising water demand and the waste water emissions due to rapid economic development and urbanization are likely to...

Ngày tải lên: 10/12/2016, 15:29

... Caspases-2, 8, 9, 10 and 12 function upstream within the apoptotic signaling pathways and are termed initiator caspases (group II) Caspases-3, 6, and 14 are activated by initiator caspases and act as the ... tetrapeptide motifs in their substrates Caspases-1, and prefer the tetrapeptide WEHD, whereas caspase-2, and have a preference for DEXD, and caspase-6, and prefer (L ⁄ V)EXD [21,22] However, a peptide ... structures reveal that non-optimal peptides for group III and optimal peptides of group I and II can bind and form favorable interactions within S2, S3 and S4 subsites of group III caspase-7 Also, a new...

Ngày tải lên: 18/02/2014, 16:20

... Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p and Pex6p involves ... presence of Pex1p and Pex6p by SDS ⁄ PAGE and immunoblot analysis with antibodies against Pex1p, Pex6p and anti-(rabbit IgG) 56 I Birschmann et al Microscopy Potassium permanganate fixation and preparation ... et al Domain function of Pex1p and Pex6p A C B D Fig Coimmunoprecipitation and two-hybrid interaction of Pex1p and Pex6p A schematic representation of the Pex1p and Pex6p constructs which were...

Ngày tải lên: 07/03/2014, 16:20

... (residues G and I), a-Galp (residues K and L), and a-GlcpN (A) The analysis of an HMBC spectrum showing intraresidual cross-correlation signals from anomeric protons to carbons C-3 and C-5 was ... at the reducing end, residues A and B) and at positions of both L,D-Hepp-residues E and F as determined by 31P,1H-COSY, as well as 1H- and 13C-chemical shift analysis The sequence of residues ... L,D-Hepp (residue H) and the inner a-Kdop (C), and between the adjacent L,D-Hepp (residue F) and the side-chain a-Kdop (residue D) were observed in the analysis of the R3 and R4 core oligosaccharides...

Ngày tải lên: 08/03/2014, 09:20

... 4-amino-5-aminoethyl-2methylpyrimidine: with a kcat and KM of 1.7 ± 0.2 min)1 and 58 ± 22 lm, respectively The F47Y variant appears to be poorly active as well: with a kcat and KM of 0.06 ± 0.006 min)1 and 68 ± 16 lm, respectively ... the phos6230 phorylation of HMP and hydroxyethylthiazole (HET), ThiD (HP0844) and ThiM (HP0845), respectively, and one responsible for the coupling of the HMP and HET moieties, corresponding ... molecule and the expulsion of the active cysteine complete the reaction Asp44 is positioned to stabilize and orient the binding of the substrate, and Tyr112, Glu205 (207 in HP-TenA) and Tyr47...

Ngày tải lên: 16/03/2014, 00:20

... forms of TbPFK-2 and investigated whether the T brucei PFK-2 and FBPase-2 enzymes are homologous to their counterparts in higher eukaryotes Results and Discussion Purification and characterization ... genome projects (T brucei, T cruzi and Leishmania major) using a query of mammalian and yeast bifunctional PFK-2 ⁄ FBPase-2 sequences Analysis of the various T brucei and L major databases surprisingly ... mammalian and S cerevisiae PFK-2 and FBPase-2 domains (and corresponding to boxed residues in Fig 3) c Overall percentage of amino acid sequence identity between the predicted trypanosomatid enzymes and...

Ngày tải lên: 16/03/2014, 22:20

... peptide and amino acids 112–169, fused to the hinge and Fc region of human IgG1 (CFC-Fc), and tested its ability to bind to ALK4 Fig FACS analysis of the interactions between Cripto and ALK4 ... VWFC (von Willebrand Factor C)-like domain in human and chicken a-1 collagen, mouse and human NELL 2, and chicken NEL, with low homology (e-value > 0.1), in addition to other Cripto and Cripto-like ... both EGFlike and VWFC domains, but, only in human Jagged and Jagged 2, Drosophila Serrate, and NELL were both domains adjacent to and in the same order as the putative EGF-like and CFC regions...

Ngày tải lên: 23/03/2014, 21:20

... V18/C279 (b1 strand), L32/F293 (b2 strand), I33/K294 (loop after b2 strand), I68/F334, L71/ V337 and L73/F339 (all in b5 strand) of D3 and I41/L304, C43/A306 (both in b3), L69/S326 and L71/L328 ... dimer The first cluster includes F70/V336 and I72/Q338 (both in b5 strand) of D3 and F27/Y289 (b2 strand), L67/M324, V70/I327 and L72/L328 (all in b4 strand) of B The second cluster consists of ... processing tasks Structural modelling of ataxin-2 First, we compiled a list of ataxin-2 homologues including the yeast homologue Pbp1 and several Lsm domains of snRNPs and other Sm and Sm-like proteins...

Ngày tải lên: 30/03/2014, 15:20

... Scalar invariants and spectral analysis Invariants of vectors or tensors Primitive invariants Trace invariants Characteristic invariants ... his lectures, and I have adopted his notation in this document Later taking Buck’s beginning and advanced finite element classes further improved my command of matrix analysis and partial differential ... Expressing the inverse in terms of powers and invariants CASE: Expressing the cofactor in terms of powers and invariants Eigenvalue problems Algebraic and geometric multiplicity of eigenvalues...

Ngày tải lên: 31/03/2014, 16:19