epstein barr virus ebv infection and the nervous system overview

... variations in EBVaGC, NPC biopsies and TWs of healthy donors in Northern China The numbers in the first row correspond to the amino acid positions and the numbers in the second correspond to the nucleotide ... Detection of Epstein- Barr virus by PCR and expression of LMP1, p53, CD44 in gastric cancer Korean J Intern Med 2004, 19:43-47 Andal N, Shanthi P, Krishnan KB, Taralaxmi V: The Epstein Barr virus and gastric ... of GCs and NPCs were collected from major hospitals of Shandong Province in the Northern China, a non-endemic area of NPC The infection of EBV in GC and NPC tissues was determined by EBV- encoded...

Ngày tải lên: 12/08/2014, 02:20

... antigen (EA) and EBV nuclear antigen (EBNA1) [11] On the other hand, aberrant antibody levels against EBV have been evidenced in the EBV- associated carcinomas due to the specific EBV gene-expression ... analysis of these EBV parameters during NPC treatment and follow-up In the most patients, the kinetics of the eight anti -EBV antibodies was consistent In 15 of the 35 patients the levels of anti -EBV ... after NPC therapy and the EBV seroreactivity declined On the other hand, the unaffected individuals from high-risk families and community controls showed no statistical differences in the antibody...

Ngày tải lên: 18/06/2014, 15:20

... exist in the nucleus, which allow for amplified expression of the therapeutic gene A standard vector derived from the Epstein Barr Virus contains the origin of replication oriP and the sequence ... replication of the vector and the Introduction expression of the therapeutic gene that is carried on the vector must occur Therefore, a better understanding of the relationship between replication and transcription ... met the oriP If that is the case, our stand that the arrest of the transcription complex along the DNA template prevents the DNA replication complex from recognizing and binding to the oriP, thereby...

Ngày tải lên: 05/10/2015, 21:24

... Epstein- Barr virus life cycle 1.2 EBV and the immune system 1.2.1 1.2.2 CD4+ helper T cell responses .6 1.2.3 EBV and NK cells 1.2.4 EBV and the human cytokine system ... comprehensive overview of the life cycle of EBV is illustrated below in Figure Humanized mouse model of EBV infection Introduction Figure Epstein- Barr virus (EBV) life cycle Epstein- Barr virus (EBV) is ... 1.2.5 Anti -EBV antibody responses The function of the anti -EBV humoral immune response is to limit the infectious virus particles and therefore, to control the spread of the virus in the late stages...

Ngày tải lên: 10/09/2015, 09:22

... particularly with selection and functional testing of individual clones In contrast, plasmids with an Epstein Barr virus (EBV) origin of replication oriP in the presence of EBV- encoded EBNA-1 protein ... of the unmodified TetR The original tetracycline repressor simply binds to a tandem of the type tetracycline operator (tetO2) operator and thus blocks transcription from the upstream cytomegalovirus ... tetracycline or doxycycline to the culture medium turns on expression: the inducer binds to the repressor, which then dissociates from the operator From the lack of interaction of the unmodified TetR with...

Ngày tải lên: 23/03/2014, 09:21

... S: Differential cellular targets of Epstein- Barr virus (EBV) infection between acute EBV- associated hemophagocytic lymphohistiocytosis and chronic active EBV infection Blood 2001, 98:1882-1888 ... Americans, and is associated with rapid progression, high morbidity and mortality It can develop after primary EBV infection or in association with chronic active EBV infection (CAEBV) Despite the name, ... analyzed data; SAP and PPP performed research, analyzed data and wrote the manuscript All authors read and approved the final manuscript VT and CA contributed equally to this work; SAP and PPP contributed...

Ngày tải lên: 10/08/2014, 23:21

... quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein- Barr virus, and human herpesvirus DNA in blood and other clinical specimens ... Bauer CC, et al: Serum Epstein- Barr virus DNA load in primary EpsteinBarr virus infection J Med Virol 2005, 75(1):54-8 45 Dehee A, et al: Quantification of Epstein- Barr virus load in peripheral ... assessing methods for the detection and quantification of EBV and other viruses [27,74,75] Conclusion This is the first reported study that uses the SYBR Green I dye on the Rotor-gene 6000™ with...

Ngày tải lên: 12/08/2014, 01:22

... expresses the EBV nuclear antigen (EBNA) gene and is characteristic of BL The latency II program expresses EBNA1 plus the latent membrane proteins (LMP) and LMP2 and is seen in Hodgkin's disease and the ... latent EBV infection can be disrupted by expression of the master regulator lytic EBV gene BZLF1 that initiates EBV replication, ultimately resulting in the assembly of new EBV particles and their ... Namalwa cells not produce EBV particles Furthermore, in Namalwa cells, the EBV genome is integrated in the human chromosome [34], while the other BL cell lines harbor the virus in episomal form...

Ngày tải lên: 20/06/2014, 01:20

... by the HLA-Dw4 DR β-1 chain and the Epstein- Barr virus glycoprotein gp110 Proc Natl Acad Sci USA 1989, 86:51045108 Lotz M, Roudier J: Epstein- Barr virus and rheumatoid arthritis: cellular and ... of latent Epstein- Barr virus infection in systemic lupus erythematosus J Immunol 2004, 172:1287-1294 116 Gross AJ, Hochberg D, Rand WM, Thorley-Lawson DA: EBV and systemic lupus erythematosus: ... blood drawn before the diagnosis of RA Nevertheless, given the ubiquity of the virus in the population, a binary assay for the presence of anti -EBV antibodies years preceding the onset of RA would...

Ngày tải lên: 09/08/2014, 07:20

... 12(10):2129-2135 Sandvej K, Peh SC, Andresen B, Pallesen G: Identification of potential hot-spots in the carboxy-terminal part of the Epstein Barr virus (EBV) BNLF-1 gene in both malignant and benign EBV associated ... contributions HFSand HSSplanned the experiment and performed most of the experiments with the help and supervision of HFS WKYselected the blocks, performed the paraffin tissues sectioning and prepared ... 32B(3):163-168 Sandvej K, Gratama JW, Munch M, Zhou XG, Bolhuis RL, Andresen BS, Gregersen N, Hamilton-Dutoit S: Sequence analysis of the Epstein- Barr virus (EBV) latent membrane protein-1 gene and promoter...

Ngày tải lên: 09/08/2014, 07:21

... Figure virus- specific T-cell response Correlation between the Epstein- Barr virus viral load and Epstein- Barr virus- specific T-cell response Correlation between the number of IFNγproducing T cells and ... purposes) gitudinal study Epstein- Barr virus load in peripheral blood mononuclear cells in the longitudinal study (a) Epstein- Barr virus (EBV) load between week (W0) and week 12 (W12) for all ... Correlation between Epstein- Barr virus load and T spots For correlation studies between the EBV viral load and EBVspecific T-cell response, 113 patients were studied (66 at week and 47 at week 12)...

Ngày tải lên: 09/08/2014, 14:21

... pattern, i.e., anti -Epstein- Barr virus- viral capsid antigen (VCA) IgM

Ngày tải lên: 10/08/2014, 22:20

... prednisone); EBER: Epstein- Barr virus encoded RNA; EBV: Epstein- Barr virus; HAART: highly active antiretroviral therapy; HIV: human immunodeficiency virus; HHV-8: human herpesvirus 8; KSHV: Kaposi ... patient data and revised the manuscript LOO did cytotechnology and revised the manuscript PPP and SAP reviewed and revised the manuscript All authors read and approved the final version of the manuscript ... [15] The co -infection of HHV-8 and EBV in this patient is interesting Both HHV-8 and EBV are g-herpes viruses and are closely related While evidence of HHV-8 is essential for diagnosis of PEL, the...

Ngày tải lên: 11/08/2014, 00:22

... the presence of the pX region, which exists between env and the 3'-LTR This region encodes several accessory genes, which include the tax, rex, p12, p21, p30, p13 and HBZ genes Among these, the ... an infected cell and a target cell induces the accumulation of the viral proteins Gag and Env, viral RNA and microtubules, and the viral complex subsequently transfers into the target cell [49] ... Provirus load and transmission The provirus load varies more than 1000-fold among asymptomatic carriers [53] Since most infected cells are considered to have one copy of the provirus, the provirus...

Ngày tải lên: 13/08/2014, 09:21

... hepatitis C [23] Furthermore, the severity of steatosis in these patients is directly related to the burden of the HCV RNA load This relationship between the HCV viral load and the magnitude of ... 54 infections with other underlying metabolic diseases In these patients, we would expect that the steatosis would only partially resolve with successful eradication of the virus and that the ... HCV genotype virus and was not reproducible with other HCV genotypes These findings all point to the ability of the HCV genotype virus to directly induce steatosis, although the mechanism still...

Ngày tải lên: 02/11/2012, 09:51

... EMSA towards the minus-strand genome, the minus-strand 3¢ UTR was the strongest competitor, followed by the random sequence of coding region and the minus-strand 5¢ UTR, similar to the competitive ... towards the plus-genome Interestingly, when the plusstrand 3¢ UTR and the minus-strand 3¢ UTR were used at the same time, the NS5B proteins bound to the minusstrand 3¢ UTR more strongly than to the ... contribute to the formation of these products A TNTase could add nontemplated terminal nucleotides to either the newly synthesized RNA or the input template Therefore, we further examined whether the purified...

Ngày tải lên: 19/02/2014, 16:20

... Mini -EBV Virus Plasmids 23 Genetic Analysis and Gene Expression with Mini -Epstein- Barr Virus Plasmids Ellen Kilger and Wolfgang Hammerschmidt Introduction Upon infection with Epstein- Barr virus (EBV) , ... on either side termed “A” and “B” The mini -EBV plasmid p1478A carries the wild-type form of the latent EBV (wt) within the identical EBV sequences “A” and “B” Homologous recombination via the ... fraction using the former method and is therefore lost if the nuclei are discarded 48 Laing et al 1.3 Northern Blotting of the EBV- Encoded Small RNAs The small RNAs of EBV, EBER-1 and -2, the VA RNAs...

Ngày tải lên: 10/04/2014, 22:31

... designed the research and supervised the study CFH was responsible for the patient samples and tissue array construction JHH and QS supported the work of IHC LXY supported the lab work XFZ and YXZ ... cases with IRS ≥ as high expression, and cases with IRS < as low expression Cell culture and plasmids The EBV- negative human NPC cell lines HONE1 and 610B, and the EBV- positive NPC cell line C666-1 ... further clarify the signaling pathways regulated by LMP1 in NPC, we investigated the association between the mTOR signaling pathway and LMP1, the expression of p-mTOR, p-P70S6K and p-4EBP1, and...

Ngày tải lên: 18/06/2014, 16:20

... survival during viral infection and on the other hand detrimental to viral replication, it is therefore thought that a balance between the two would determine the outcome of the infection in host ... that the ability of viruses to regulate cellular responses to infection is a key determinant for the physiological consequences of infection Since the activation of the UPR is on the one hand ... HCV [19] The discrepancy of XBP1 induction between these viruses might reflect the differences in infection patterns of these viruses; while HCV usually causes chronic infection, JEV and Dengue...

Ngày tải lên: 18/06/2014, 18:20

... in the nucleus (Fig 3) The serology detected an EBV infection, with viral presence on the nasal and pharyngeal smears and on the blood polymorphonucleates Tests for HHV6 and VRS were negative The ... nasopharyngeal carcinoma cells to Epstein- Barr virus infection in vitro Laboratory Investigation 2000, 80(8):1149-60 Arrand JR, Rymo L: Characterization of the major Epstein- Barr virus- specific RNA in Burkitt ... as compared to Epstein- Barr virus serology Anticancer Res 1981, 1:187-94 Neel HB III, Pearson GR, Weiland LH, et al.: Application of Epstein- Barr virus serology to the diagnosis and staging of...

Ngày tải lên: 19/06/2014, 08:20