eps8 an adaptor protein acts as an oncoprotein in human cancer

... Telomestatin BRACO-19 BRACO-19 RHPS4 A431 human epithelial carcinoma MDA-MB-231 human breast cancer MIA PaCa-2 human pancreatic cancer UXF1138L human uterine carcinoma M14, LP, LM melanoma RHPS4b ... on haematological cancers Notable findings include that of single-agent activity for RHSP4 in a metastatic melanoma model, as well as in a melanoma line resistant to the platinum drug DDP [56] ... 22 ligand telomestatin inhibits POT1 binding to telomeric sequences in vitro and induces GFP–POT1 dissociation from telomeres in human cells Cancer Res 66, 6908–6912 Gomez D, Wenner T, Brassart...

Ngày tải lên: 06/03/2014, 09:22

... T, Thun MJ: Cancer statistics, 2008 CA Cancer J Clin 2008, 58:71-96 Sung JJ, Lau JY, Goh KL, Leung WK: Increasing incidence of colorectal cancer in Asia: implications for screening Lancet Oncol ... that CK2a protein expression levels were increased in both CRC and colorectal adenoma, and CK2a expression was much higher in CRC than in adenoma, suggesting that CK2a may be involved in the progression ... Role of protein kinase CK2 in phosphorylation nucleosomal proteins in relation to transcriptional activity Mol Cell Biochem 1999, 191:135-142 18 Ruzzene M, Pinna LA: Addiction to protein kinase CK2:...

Ngày tải lên: 18/06/2014, 19:20

... expressing NS1 protein were stained with annexin V-FITC and PI and analyzed by flow cytometry As showed in Fig 2B, H5N1 NS1-transfected cells were 2.33% Annexin V+/PI- (early apoptosis) and 17.61% Annexin ... Expression of caspase-9 and caspase-3 The expression of caspase-9 and caspase-3 in NS1transfected A549 cells were measured by Western-blot analysis Briefly, monolayer of cells transfected with ... V-FITC and negative for PI (FITC+/PI-) 0h 12 h Caspase-3 Caspase-9 24 h 48 h 12Kd 17Kd -actin Figure Caspase-3 and caspase-9 activation in NS1-transfected A549 cells The cells were transfected...

Ngày tải lên: 12/08/2014, 04:20

... LAT, and tyrosine kinase Lck were reported to be coclustered in discrete T cell plasma membrane microdomains The integrity of these microdomains was dependent on protein protein interactions based ... thus include: short extracellular domain, single transmembrane domain, intracellular domain containing signalling-relevant motifs, such as potentially phosphorylated tyrosine motifs, polyproline ... TCR was dependent on CD2 [89] Stimulation of EphrinB1receptor (a receptor tyrosine kinase interacting with the transmembrane ligand EphrinB1) led to increased LAT phosphorylation and p44 ⁄ 42 and...

Ngày tải lên: 18/02/2014, 04:20

... cancer- specific miRNAs in many types of cancer, including B-cell chronic lymphocytic leukemia [11], colorectal cancer [12,13], lung cancer [14], breast cancer [15], and brain cancer [16,17] A recent ... of PPP6C resulted in a significant decrease in the proportion of cells in G1-phase and an increase in the proportion of cells in S-phase These findings indicate that PPP6C decreases the proliferation ... different functions in different tissues; for instance, miR-9 is upregulated in breast cancer cells [30], but downregulated in human ovarian cancer [31] In addition, different miRNAs can target the...

Ngày tải lên: 14/03/2014, 23:20

... A and luciferase bioluminescence H Fraga et al Fig The stabilizing effect of CoA on rey luciferase bioluminescence Mixtures containing ATP and LH2 were injected into other mixtures containing ... concentration in the eluent and the ux rate (which was 4.9 mm and mLặmin)1, for the case of CoA, and mm and 1.7 mLặ min)1, for the case of dephospho-CoA), the aliquots were analysed as described above In ... luciferase C-terminal domain Biochemistry 44, 13851393 Wang CY & Andrade JD (1996) Surfactants and coenzyme A as cooperative enhancers of the activity of rey luciferase In Bioluminescence and Chemiluminescence:...

Ngày tải lên: 16/03/2014, 23:20

... Furthermore, a-defensin induces interleukin secretion in the human intestinal cell line T84 via signaling cascades that involve both NF-jB and p38 mitogen-activated protein kinase The mechanism of secretion ... hemocytes and are involved in the storage and release of defense molecules, including serine protease zymogens, a clottable protein coagulogen, protease inhibitors, antimicrobial peptides, and lectins ... mastoparan, although we have not yet identified horseshoe crab G protein( s) involved in hemocyte exocytosis The binding parameters of mastoparan and tachyplesin to bovine G protein were determined...

Ngày tải lên: 30/03/2014, 20:20

... streptomycin; and were found to be of 95% purity as determined by immunocytochemical staining with ox42 and anti-glial fibrillary acidic protein (GFAP) antibody To investigate the anti-inflammatory ... LPS-induced NO release in microglia and astrocytes, both in a dose-dependent manner (Figure 2B) Furthermore, LPS increased the protein expression of iNOS both in microglia (Figure 2C) and astrocytes ... viability was determined by MTT assay (A) The level of nitrite in the culture medium was determined by the Griess reaction (B) iNOS protein levels in microglia (C) and astrocytes(D) were measured...

Ngày tải lên: 19/06/2014, 22:20

... line 293T [54], human hepatoblastoma cell line HepG2 [55], human hepatoma cell line Huh7, and human astrocytoma cell line U251MG [56] were maintained in Dulbecco's modified Eagle minimum essential ... Y, Kanbe K, Liu HY, Jinno A, Kitamura T, Hoshino H: An orphan G protein- coupled receptor, GPR1, acts as a coreceptor to allow replication of human immunodeficiency virus types and in brain-derived ... (NISSUI Co Ltd., Tokyo, Japan) containing 10% fetal calf serum (FCS) The human osteosarcoma cell line HOS [51] and CD4-transduced human glioma cell line U87/CD4 [52,53], as well as NP-2/CD4 [49], NP-2/CD4/...

Ngày tải lên: 13/08/2014, 05:21

... 2005) and promoter hypermethylation (Kim et al 2005) In a substantial fraction of gastric cancer cases in which RUNX3 was expressed, the protein was mislocalized in the cytoplasm as an inactive ... nuclear translocation of RUNX3 in a gastric cancer cell line (Ito et al 2005) Inactivation of RUNX3 is detected in human cancers such as lung cancer (Sato et al 2006, Yanagawa et al 2007), glioblastoma ... activated in pancreatic cancer (Wada et al 2004) and as part of this thesis, in BCC (Salto-Tellez et al 2006) 25 Table RUNX3 in human cancers Type of cancer Status Mechanism References Lung cancer...

Ngày tải lên: 10/09/2015, 15:50

... allowing for easier release of product molecules and leading to an increased turnover rate Previous work has shown that lipoprotein lipase (LPL) interacts strongly with, and in fact is inhibited ... Lookene A, Zhang L, Tougu V & Olivecrona G (2003) 1,1¢-bis(anilino)-4-,4¢-bis(naphtalene)-8,8¢-disulfonate acts as an inhibitor of lipoprotein lipase and competes for binding with apolipoprotein CII ... proteins has been not been demonstrated [5,6,17] Data are emerging that point to perilipin as a key player in directing proteins involved in lipolysis to a subset of lipid droplets [5] Interestingly,...

Ngày tải lên: 18/02/2014, 11:20

... stress-activated protein kinase, an enhanced HSF (heat shock element) interaction and the increased synthesis of HSP-70 [28] Ca2+ can be released from internal Ca2+ stores, through channels in the endoplasmic ... phospholipase C and phospholipase A2 activity in mammalian cells J Cell Physiol 155, 248–256 Ding XZ, Smallridge RC, Galloway RY & Kiang JG (1996) Increases in HSF1 translocation and synthesis in human ... (BA) and heptanol (HE), we induced a disordering effect by weakening the van der Vaals interactions between the lipid acyl chains [13] As in the case of heat stress, the initial fluidity increases...

Ngày tải lên: 07/03/2014, 12:20

... ung th in vitro, thấy quercetin làm thoái giáng đột biến p53 - protein, làm ngừng chu kỳ tế bào giai đoạn G1 , ức chế tyrosin - kinase, ngăn chặn hoạt tính protein - ras In vitro, quercetin ức ... gemcitabin topotecan Bromelain Là hỗn hợp protease chứa S, lấy từ thân dứa (Ananas conosus L.Merr, họ Bromeliaceae) Từ 1957, hàng trăm 98 nghiên cứu công bố áp dụng điều trị bromelain, nh kìm hãm ... In vitro, quercetin ức chế tyrosin - kinase ngời ung th, ức chế phát triển khối u, làm tăng thời gian sống sót động vật mang u ác Trên mô hình thực nghiệm, quercetin có hiệu lực số dạng ung th,...

Ngày tải lên: 20/03/2014, 03:20

... Doxorubicin Doxorubicin, platinum-based drugs, Vinblastin, Vincristin, Topotecan, Paclitaxel In vivo In vivo Chemistry, structural analysis and in vitro In vitro / in vivo Clinical trials (Phase I) In ... to human blood cancer (B-cell lymphoma) were labelled with an internalizing anti-CD19 ligand rather than a non-internalizing anti-CD20 ligand19 In contrast, targeting nanocarriers to non-internalizing ... World Cancer Report (World Health Organization Press, Geneva, 2003) Cancer Facts & Figures 2007 (American Cancer Society, Atlanta, 2007) Duncan, R Polymer conjugates as anticancer nanomedicines...

Ngày tải lên: 28/05/2014, 15:07

... now or in the future? Is such an organization financing this manuscript? If so, please specify No Do you hold any stocks or shares in an organization that may in any way gain or lose financially ... with propofol and fentanyl and the airway was secured with a laryngeal mask Anaesthesia was maintained with a propofol infusion and intermittent boluses of fentanyl Routine monitoring included heart ... please specify No Do you have any other financial competing interests? If so, please specify No Non-financial competing interests Are there any non-financial competing interests (political, personal,...

Ngày tải lên: 10/08/2014, 10:20

... here is very important They have not mentioned anything about the ventilator rate and tidal volume setting in their particular case in relation to capnogram recording Hypocarbia can result from several ... Plexus and Peripheral Nerve Injury 2008, 3:20 http://www.jbppni.com/content/3/1/20 depth and mentioning of drug dose are important Nothing was mentioned to rule out this possibility in the case ... mentioned something about progressive reduction in ETCO2 level in subsequent capnogram tracings But they have not given any valid explanation to the cause of this occurrence Ventilator setting here...

Ngày tải lên: 10/08/2014, 10:20

... and Peripheral Nerve Injury 2008, 3:21 http://www.jbppni.com/content/3/1/21 With increase in the amplitude of current, there is further increase in the force of diaphragmatic contraction (akin ... fall in ETCO2 We hope the response addresses the concerns of the reader Thanking you Hemant Bhagat, Anil Agarwal, Manish S Sharma Competing interests The authors declare that they have no competing ... competing interests They did not receive any grants nor they have any vested interests in the equipment described References Akcali DT, Ozkose Z, Yardim S: Do we need bispectral index monitoring during...

Ngày tải lên: 10/08/2014, 10:20

... 'wind up' phenomena in CRPS forms a strong basis for the use of Ketamine to block the cellular mechanisms that initiate and maintain these changes Both subanesthetic infusions and Ketamine induced ... allodynia Ketamine has been administered orally, as an ointment, intravenously, subcutaneously, epidurally and intramuscularly in a number of case reports in literature[17-19] Ketamine doses for ... edema, joint stiffness nail changes, skin atrophy, mirror image changes in opposite hand adjuvant were tried unsuccessfully In the 10th week, a trial of SGB with 10 mg Ketamine as an adjuvant was given...

Ngày tải lên: 10/08/2014, 10:20

... determined For instance, an increase or decrease in ph of acidic organelles and the changes in lysosome numbers by compound toxicity results in a decrease or an increased of fluorescence intensity, ... SiO2NP did not induce any lysosomal mass/pH staining intensity changes for all tested doses in the THP-1 cell line, (Figure 4C, 4E) The lysosomal mass/ pH staining intensity was increased in A549 cells ... of cancers, including sarcomas, some carcinomas (e.g small cell lung cancer, and ovarian cancer) , lymphomas, and germ cell tumours [48] The experimental layout for the automated microscopic analysis,...

Ngày tải lên: 11/08/2014, 00:23