ecm protein binding induces integrin receptor clustering on the cell surface

... to the dimer and monomer, respectively, and the elution prole was not dependent on the protein concentration (C) and (D) The elution prole of PC-(BC) was markedly dependent on the protein concentration: ... the DNA sequences The correct mutation of the second amino acid residue, arising from the introduction of an NcoI site in the start codon region, to glutamate from the original lysine, was conrmed ... 462 on the basis of the reasoning described in the Results section The polypeptide chain was divided into two at this point by placing a stop codon or an initiation codon for the expression of...

Ngày tải lên: 16/03/2014, 16:20

... and the ST mutant of UK3 PVX elucidate the role of the PVX CP N-terminal region in determining the physicochemical properties of the virion surface, preservation of the virion integrity, and the ... hydrolysis correspond to the N-terminal regions of the PVX CP subunits As can be seen in Fig 3, the supernatant from the ST mutant contains one major peptide fraction and the supernatants from the UK3 ... respectively, and the latter has alanine and threonine (Fig 1).] The elution of the ST mutant peptide at higher acetonitrile concentrations than the UK3 peptide may be due to the substitution of 11 serine...

Ngày tải lên: 23/03/2014, 13:20

... for the interaction and the saturation of the low affinity binding site Consequently, the binding of PTN to the low affinity -binding site should be responsible for the inhibitory effect of PTN on ... 618 cells pretreated with PTN or the nucleolin -binding HB-19 pseudopeptide, before adding the virus preparation on cells The fact that CHO K1 cells not express the HIV receptor CD4 or the coreceptors ... Fig The effect of the nucleolin binding molecules on the binding of PTN to the low and high affinity -binding site (A) Inhibitory effect of HB-19 on the binding of the 125 I-labeled PTN to the...

Ngày tải lên: 30/03/2014, 20:20

... suggested that they play a role in the prevention of infection by microbes [11,12] or RNA-viruses.[13] They might also control the hypothesized cell- to -cell communication mediated by the release ... http://www.gvt-journal.com/content/4/1/4 ase activities The major ribonuclease activity on the human skin surface is very similar in size (~13 kDa) to the one on the mouse skin surface Moreover, some sub-dominant ribonuclease ... in blood plasma and the one mediating the main ribonuclease activity on skin surface (Fig 1) To conclude, both for mice and humans, the ribonuclease activity on the skin surface is specific for...

Ngày tải lên: 14/08/2014, 19:22

... depending on the mineralogical and chemical composition of the activated bentonite, type and concentration of the inorganic acid, used in the process and also temperature and time of activation The ... activation with a H2SO4 content of 50% by mass The crystallinity of the non-clay minerals are not affected by the acid activation process 3.2 Nitrogen adsorption and desorption isotherms The N2 ... desorption isotherms The hysteresis phenomenon becomes more prominent with acid activation because the amount of mesopores increases by the structural deformation Due to the same reason, capillary condensation...

Ngày tải lên: 09/10/2015, 00:00

... on the cell surface of developing muscle cells, is a PTPr -binding protein Nucleolin expression correlates with the location of PTPr -binding sites on developing muscle, and nucleolin -binding proteins ... nucleolin is present on the surface of a variety of cell lines [42,48,52,55,56] and on the surface of endothelial cells [57,58] To address whether nucleo4674 lin is also present on the surface of developing ... reaches the cell surface The clustering of nucleolin on cell surfaces is nonetheless dependent on an intact actin cytoskeleton, to which it must attach through an unidentified, integral membrane protein...

Ngày tải lên: 19/02/2014, 05:20

... formation, in fractions and 7, was abolished by the V19 mutation, verifying the nature of these complexes (Fig 5) These data indicate that the CCV1 Fig Gel filtration fractionation of the VEGF ... expression in the cytoplasm, in conjunction with another singlestrand RNA/DNA binding protein, PTB Conserved CSD/PTB binding sites in the VEGF mRNA 5¢- and 3¢-UTR The 5¢- and 3¢-UTR of the VEGF mRNA ... consensus CSD site or the downstream PTB sequence CSD proteins therefore recognize not only the expected consensus RNA sequence but also the VEGF PTB binding sequences The binding of CSD proteins to PTB...

Ngày tải lên: 19/02/2014, 12:20

... contribution to the reduction potential of the centres is small [40,41], and also with the experimental observation for monohaem cytochromes that the effect of anion binding or complex formation on the reduction ... undisturbed by the replacement of Fe by Zn in the rubredoxin given the identical structures of the two protein forms [38,39] Therefore, at the concentrations used in the NMR experiments over 90% of the ... of the following redox mediators was added to the protein solution at pH 7.0: indigo tetrasulfonate, indigo trisulfonate, indigo disulfonate, anthraquinone-2-7-disulfonate, anthraquinone2-sulfonate,...

Ngày tải lên: 19/02/2014, 17:20

... binding proteins The preparation of the small binding proteins with binding function further allows us to increase the binding strength by multibinding approaches, constructing multispecific proteins ... describes the methodology for elucidating protein protein interactions and selecting specific binders to novel target proteins, and the first and second minireviews focus on the detection of protein protein ... function [30]: the grafted Fab had agonist activity caused by the dimerization of the TPO -binding peptide The combination of grafting and local library methods is suitable for generating binding proteins...

Ngày tải lên: 06/03/2014, 11:20

... epitope in the ic3 of the D2 receptor induced alterations in the cellular localization of the resulting receptor proteins If not for confocal microscopy, which allowed for the visualization of receptor ... fusion proteins, these receptors were found in different parts of the cell The D2R3 mutant was localized inside the cell, whereas the D1 receptor was found in the plasma membrane However, when the ... the parameters, and the fractional contribution of the additional lifetime was close to zero Figure shows the typical time-dependent donor decays for the D1–CFP bearing donor alone and with the...

Ngày tải lên: 07/03/2014, 03:20

... compare the fractional contributions of the uorescence of the red and blue components from the solute quenching experiments by using the fractional contributions of the short and long lifetimes of the ... CRP binding to the different DNA sequences leads to long-distance conformational signal transmission from the C-terminal domain to the N-terminal domain of the protein Furthermore, we present the ... characterized by the location of the CRP -binding site centred at position )61.5 In the case of class II promoters, such as galP1, the CRP -binding site is located at position )41.5 The activation of the transcription...

Ngày tải lên: 07/03/2014, 16:20

... order The reachability distance is given on the y axes, and the objects (i.e binding site representations) are numbered on the x axes Clusters correspond to concave regions in the plot After the ... participating in the bond were indicated by their one-character code; nonbinding amino acids were indicated by ‘-’ As our purpose was to deal with only the binding sections, the pre- and postfixes consisting ... stored the binding sites found in the PDB in a compact data structure The definition of binding sites A binding site is defined as a set of atom pairs; the first atom of the pair belongs to the protein, ...

Ngày tải lên: 15/03/2014, 10:20

... for h In the nontransfected control cells before heat shock, Smac was detected in the motichondrial fraction but not in the cytosolic fraction, consistent with its known subcellular location After ... Smac from mitochondria into the cytoplasm Concordantly, the protein level in the mitochondria was significantly decreased In the transfected cells, HSP70 over-expression inhibited the release of ... of HSP70 expression because the clone with higher HSP70 expression demonstrated a more significant reduction of the apoptotic cell population (Fig 2B) Furthermore, over-expression of HSP70 displayed...

Ngày tải lên: 16/03/2014, 01:20

... ANP binds to the extracellular domain of the NPR-A dimer and induces a conformational change of the intracellular KHD domain Second, ATP binds to the newly configured KHD Third, ATP binding increases ... in the KHD Thus, the function of this domain, and the dual regulation by ATP and phosphorylation, are not fully understood Based on these observations, the current model for NPR-A activation ... Interestingly, the activation by ATP ⁄ ANP for the WT receptor in these membrane preparations was the highest activation ever obtained for this receptor (% 67% 5574 125 I-labeled ANP binding We and others...

Ngày tải lên: 16/03/2014, 23:20

... solutions were prepared The first was the gel-filtration elution buffer containing the 43-kDa protein incubated for 30 at room temperature; and the second was the gel-filtration elution buffer containing ... determine the potential association of the 43-kDa protein, as the receptor of the physiologically active peptide usually forms an oligomer to activate the function of the receptor [12] When the 43-kDa ... concentration of 1.0 mM, cells were grown for a further h and harvested by centrifugation at 6000 g for 10 at °C The cells were suspended in 40 mL of BugBuster proteinextraction reagent The cell...

Ngày tải lên: 17/03/2014, 03:20

... analyzed in parallel during protein injection Additionally, thioredoxin was injected at high concentrations (3.5 lM) to rule out nonspecific interactions of the fusion protein of SOCS-3 Curves were ... precipitation the proteins were subjected to Western blot analysis using a polyclonal anti-(SOCS-3) Ig to detect coprecipitated SOCS-3 we also considered the binding of the receptor peptide corresponding ... addition the side chain of R94 is able to build up a hydrophobic contact with the aromatic ring of the phosphotyrosine at position Y+0 (contact shown as a red ÔhÕ in Fig 6B) Taken together, the contributions...

Ngày tải lên: 24/03/2014, 00:21

... blocked the binding of BtR175 We discuss the location of the BtR175 -binding site on Cry1Aa toxin on the basis of location information obtained with the epitopes of mAbs that block the binding ... shown) These results suggest that the BtR175 -binding and BmAPN1 -binding sites are not the same In conclusion, the BtR175 -binding site is on the same face of Cry1Aa toxin as the BmAPN1 -binding ... single exponential fit of the binding response curve, and the apparent dissociation rate constant, koff (s)1), was calculated from the dissociation response curve The dissociation constant, kdiss...

Ngày tải lên: 24/03/2014, 00:21

... that the loss of binding ability is derived from disruption of the toxin conformation caused by the mutation introduced distant from the receptor -binding site Indeed, mutations in the region distant ... the receptor -binding region of Cry11Aa [13,40] These observations indicate that a conformational change and subsequent oligomerization can be triggered if some proteins bind to the receptor -binding ... results of the present study reveal that the region on the binding interface of the Cry1Aa molecule may be responsible for binding Our data suggest that the binding region is located on or around...

Ngày tải lên: 29/03/2014, 22:21

... expression levels observed Mutations in ICLs affect the receptor ligand binding site The receptor equilibrium binding affinity (Kd) reflects the conformation of the extracellular ligand binding ... determinants in the intracellular loops (ICLs) of the receptor are responsible for self-maintenance, for the recruitment and activation of the G protein, or for the initiation of the desensitization process ... insertion into the genome of the host cell line affecting the expression level per se In total, only one mutant (construct ⁄ 1) of the 14 constructs displayed 3498 Fig Internalization of [3H]BK Cells...

Ngày tải lên: 29/03/2014, 23:20

... addition to the above observations, suggest that the aIIb 313–332 region is important, not only for fibrinogen binding but also for platelet activation The rationale of this study was based on the ... were recognized by the activated aIIbb3 integrin Therefore, in the context of this study, the fine mapping of the fibrinogen -binding domains on the aIIb subunit was accomplished and their potential ... inhibition of fibrinogen binding to the activated receptor, as well as platelet aggregation, could be caused by their interaction with fibrinogen The latter is further supported by the results of the...

Ngày tải lên: 31/03/2014, 07:20