dna  rna  protein

... published 50S proteins 50S rRNA tRNA, E-site tRNA, P-site tRNA, A-site mRNA 30S proteins 30S rRNA Fig Structure of the 70S ribosome The structure of the ribosome of Thermus thermophilus (Protein Data ... are compared, neither the DNA nor the protein undergo large FEBS Journal 278 (2011) 1643–1650 ª 2011 The Authors Journal compilation ª 2011 FEBS Transient protein DNA ⁄ RNA interactions F J Blanco ... specificity at protein DNA interfaces Nucleic Acids Res 37, e73 FEBS Journal 278 (2011) 1643–1650 ª 2011 The Authors Journal compilation ª 2011 FEBS 1649 Transient protein DNA ⁄ RNA interactions...

Ngày tải lên: 14/02/2014, 19:20

... (1) Chỉ có DNA cấu tạo theo nguyên tắc đa phân mà đơn phân nucleotit (2) Nucleotit DNA ARN có đường pento, axit photphoric bazo nito (3) DNA có đường C5H10O5 ARN có đường C5H10O4 (4) DNA thường ... phân tử protein gồm chuỗi polipeptit có chiều dài 1500A0 Số liên kết peptit có protein : A.500 B.499 C.498 D.497 Câu 24 Tổng số axit tạo thành phân tử protein 14880 axit amin Trong phân tử protein ... nito (3) DNA có đường C5H10O5 ARN có đường C5H10O4 Sai : DNA có đường C5H10O4 ARN C5H10O5 (4) DNA thường có nhiều đơn phân đến hàng triệu ARN có đơn phân có vài chục 10 SĐT : 0902651694 (5) DNA mạch...

Ngày tải lên: 22/02/2014, 23:58

... trình S n m i RNA = s n okazaki + s tái b n D ng m i nh s nucleotide phân t tRNA trình d ch mã Chú ý phân t mRNA có nucleotide mã k tRNA nh t i b ba D ng m i nh kh ng protein b c M protein = (kh ... ch a N15: B C 64 D 32 nh DNA ( N15 ) s nucleotide hai m DNA ( N14 t o DNA n, s DNA c t o ra: 2k = 26 = 64 + S DNA có hai m ch hoàn toàn m i ch ch N14 : 2k = 62 phân t DNA vùng nhân ch a N15 = ... Bài Phân t DNA chuy n nh ng vi khu sau l t A 62 n Tài 20 = 355 ( Nu ) = 745 ( Nu ) vùng nhân c a vi khu n E.coli ch ch a N15 phóng x N u ng có N14 m i t bào vi khu n E.coli o phân t DNA vùng nhân...

Ngày tải lên: 12/10/2014, 17:25

... TÁI BẢN DNA • Protein nhận biết bám vào khởi điển để từ hình thành nên phức hợp mở ( E.coli : protein dna A) • DNA gyrase: mở cuộn DNA siêu soắn phái trước chạc tái • Helicase: tháo xoắn DNA sợi ... : protein dnaA protein rep) • Protein SSB: bám vào DNA sợi đơn helicase tách ra, giữ tạm thời không dích trở lại mà nhờ mỗ sợi đơn làm khuôn cho tái • Primase : tổng hợp RNA mồi (E.coli : protein ... RNA mồi (E.coli : protein dna G) CÁC ENZYME THAM GIA TÁI BẢN DNA • Primase : tổng hợp RNA mồi (E.coli : protein dna G) • Các DNA polymerase xúc tác cho việc tổng hợp DNA nhờ có hoạt tính xúc tác:...

Ngày tải lên: 23/06/2016, 20:48

... people protein vs tree protein?  The answer is DNA! The species-particular DNA sequences produce the species-particular proteins  GENES code for proteins  GENES are long strands of DNA on ... at the ribosomes  DNA and protein synthesis, then, happens this way:  DNA sequence codes (how letters are put together) produce messenger RNA sequence codes  The messenger RNA leaves the nucleus ... the nucleus of eukaryotic organisms  How does DNA code for protein synthesis? First-what is protein synthesis”?  It’s building, or assembling, a protein molecule from amino acids  Amino acids...

Ngày tải lên: 13/03/2014, 16:36

... growing protein chain 3) When protein production is complete, the ribosome releases the protein chain DNA and Protein Synthesis - Summary DNA and Protein Synthesis Practice making mRNA using the DNA ... GCA Alanine DNA and Protein Synthesis But… How does the information get from the DNA to the cytoplasm? mRNA DNA and Protein Synthesis - Transcription Transcription: 1) DNA unzips 2) mRNA (messenger ... ribosome in the cytoplasm DNA and Protein Synthesis - Translation Translation: 1) rRNA (ribosomal RNA) attaches to mRNA and starts reading the codons 2) tRNA (transfer RNA) – carries amino acids...

Ngày tải lên: 13/03/2014, 16:38

... 2.1.1.2 MicroRNA It is known that mRNA is processed before it is used as template for protein synthesis, and this is done through the process of RNA interference Double stranded RNA (dsRNA) known ... interfering RNA (siRNA) was found to bind to their complementary mRNA counterpart, leading to the selective silencing or knockdown of specific proteins Firstly, as shown in Scheme 2.1, microRNA (miRNA) ... Pyrrole-imidazole Quantum dots RNA- induced silencing complex Surface enhanced Raman scattering xiv siRNA SNPs ssDNA TBE Small interfering RNA Single nucleotide polymorphisms Single strand DNA Trisborate-EDTA...

Ngày tải lên: 09/09/2015, 11:16

... For the Transient RNA protein interactions in RNA folding human mRNA-binding protein hnRNP A1 [61], the Xenopus laevis protein X1rbpa [54], the trypanosome guideRNA-binding protein RBP16 [62], ... of proteins have been found to promote RNA folding: (a) specifically binding proteins, which recognize and bind certain RNAs and thus stabilize the RNA structure, thereby forming a stable RNA- protein ... appropriate RNA FEBS Journal 278 (2011) 1634–1642 ª 2011 The Authors Journal compilation ª 2011 FEBS M Doetsch et al Transient RNA protein interactions in RNA folding Fig A generalized model for proteins...

Ngày tải lên: 14/02/2014, 19:20

... aggregative with aberrant DNA NC SSC Aggregative proteins LD DNA affinity Mono Q Marker NF Separated fractions no DNA relaxed A Aggregation-coupled DNA end-joining (kDa) 225 150 100 75 DNA- PKcs 180 k 170 ... Likewise, DNA- PKcs promoted predominant aggregation of ssDNA when incubated at relatively low concentrations At higher ratios of protein to DNA, DNA- PKcs started to interact with nicked and linear DNA ... through protein protein interaction, Ku operates to specifically promote the aggregation of DNA- PKcs on DNA ends The previous gel-shift study indicated that highly purified DNA- PKcs binds to linear DNA...

Ngày tải lên: 19/02/2014, 06:20

... enters DNA with both strands passing through the central channel of DnaB (Kaplan, 2000) The translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis We constructed the DNA substrate ... separate DNA unwinding by DnaB helicase from its ATP-dependent locomotion on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and 3’ blunt end, DnaB ... structure of the protein -DNA complex is different at the core complex as contrasted with that of the adjacent auxiliary complex The crystal structure of Tus bound to Ter DNA showed a bi-lobed protein...

Ngày tải lên: 06/03/2014, 23:20

... XPC-binding domain of hHR23B many reports suggest that these proteins stabilize the XPC protein by protecting it from 26S proteasomedependent protein degradation [5–7] Another biochemical analysis ... XPC protein during the affinity fractionation of mammalian crude extract, using an immobilized glutathione-S-transferase (GST)– S5a fusion protein [17] S5a can bind to both polyubiquitinated proteins ... either the mHR23A or mHR23B gene showed that these two proteins are functionally redundant in terms of response to DNA damage by UV light The XPC protein was not detected in the double knockout cell...

Ngày tải lên: 07/03/2014, 17:20

... 70-kDa protein is the major DNA binding component and the 45-kDa protein may associate with the DNA bound protein complex through protein protein interaction Most interestingly, another protein ... D-TERM DNAs As shown in Figs D-TERM DNA specifically 1134 V Camasamudram et al (Eur J Biochem 270) Ó FEBS 2003 Fig Protein binding specificity and protein contact sites of the D-Term DNA (A) DNAse ... that the 45-kDa protein is a weak DNA binding protein while the 70-kDa species is a high affinity DNA binding protein Under identical experimental conditions, the Mut1 D-TERM DNA probe with nucleotide...

Ngày tải lên: 08/03/2014, 08:20

... (only in RNA) - Cytosine - Guanine DNA vs RNA  DNA 1- Deoxyribose sugar 2- Bases: Adenine, Thymine, Cytosine, Guanine 3- Double-stranded helix arrangement  RNA 1- Ribose sugar 2- Bases: Adenine, ... forming a doublehelix that winds around a central axis How DNA Works 1- DNA stores genetic information in segments called genes 2- The DNA code is in Triplet Codons (short sequences of nucleotides ... containing RNA Nucleotides Composition ( parts): 1- Ribose sugar (with O in 3rd carbon) 2- Phosphate group 3- One of types of bases (all containing nitrogen): - Adenine - Uracyl (only in RNA) - Cytosine...

Ngày tải lên: 13/03/2014, 16:34

... stable Other kinds of RNA • Ribosomal RNA, rRNA, is RNA that becomes part of the ribosome, the big molecular machine responsible for synthesizing proteins • Transfer RNA, tRNA, is used to bring ... ribosome during protein synthesis • Micro RNAs (mRNAs) are important in regulating gene expression • others Transcription involves the synthesis of rRNA from DNA using RNA polymerase • RNA polymerase ... ways: Different kinds of RNA polymerases, depending on whether the product is protein or RNA RNA polymerase requires a number of helper proteins to bind to DNA and initiate RNA synthesis transcription...

Ngày tải lên: 13/03/2014, 19:38

Ngày tải lên: 15/03/2014, 13:08

... enters DNA with both strands passing through the central channel of DnaB (Kaplan, 2000) The translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis We constructed the DNA substrate ... separate DNA unwinding by DnaB helicase from its ATP-dependent locomotion on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and 3’ blunt end, DnaB ... structure of the protein -DNA complex is different at the core complex as contrasted with that of the adjacent auxiliary complex The crystal structure of Tus bound to Ter DNA showed a bi-lobed protein...

Ngày tải lên: 17/03/2014, 21:20

... of proteins Small globular proteins Adv Protein Chem 33, 167–241 30 Cobos, E.S., Filimonov, V.V., Galvez, A., Maqueda, M., Valdivia, E., Martinez, J.C & Mateo, P.L (2001) AS-48: a circular protein ... strategies for increasing the thermal stability of proteins Materials and methods Overproduction and purification of HU proteins Wild-type HU protein from T maritima (HUTmar-wt) and its mutant ... and the mutant was less stable than the wild-type protein As might be expected, the Tm values of both proteins increased concomitantly with protein concentration (Fig 3), indicating that the...

Ngày tải lên: 23/03/2014, 12:20

... levels of maternal and paternal genomes during preimplantation development Development 1991: 113, 119-127 15 16 Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and ... work is properly cited 4 Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and Histones to Diseases and Treatment DNA methylation generally occurs when a cytosine ... Polycomb-group protein target genes, which suggests they probably have common epigenetic mechanisms driving them [68-70] Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA...

Ngày tải lên: 24/03/2014, 02:21

... coli protein DiaA has been shown to interact with the initiator protein DnaA and this interaction is essential for DNA replication in Helicobacter [14,15] One protein that is central to the DNA ... less conserved and is responsible for protein protein interaction [18,19] The proteins that may interact with SSB include DNA polymerase, RNA polymerase and DNA helicases [20– 22] Although, no ... our knowledge on SSB proteins from a slow growing pathogenic bacteria and offer great potential to study DNA protein and protein protein interaction that is central to the DNA replication machinery...

Ngày tải lên: 30/03/2014, 02:20

... initiation of cellular responses to DNA damage [31–34] Recently, we have shown that DNA- PK is activated from structured single-stranded DNA (ssDNA) and hairpin DNA ends resembling replication and ... contribution of p53 DNA binding to structured DNA, we quantified the absolute levels of phosphorylation of p53wt with p53S15A/S37A in the presence of dsDNA Utilization of dsDNA minimizes DNA binding by ... identification of two sites of DNA- PK phosphorylation in the N-terminus of p53, Thr18 and Ser9, which are preferentially phosphorylated by DNA- PK when p53 and DNA- PK are colocalized to ssDNA These results...

Ngày tải lên: 30/03/2014, 15:20