differentiation induction in malignant cells as reverse transformation

... for 90 minutes in CO2 incubator, at 37°C, in the dark After that, cells were washed in PBS 1× and cultured protected from light Stained stem cells were used in co-cultures of DMD muscle cells and ... stem cells transplantation in murines [35], calling the attention for the difficulty in the interpretation of published reports as well as on our own IF results Moreover, in most cases, it was ... Medicine 2009, 7:6 http://www.translational-medicine.com/content/7/1/6 CD34+ Figure stem cells transdifferentiated in muscle cells in vitro CD34+ stem cells transdifferentiated in muscle cells in...

Ngày tải lên: 18/06/2014, 15:20

... restored the intracellular calcium concentration, the increase in Ins(1,4,5)P3 was sustained In addition, the subsequent increase in calcium was less effective at enhancing the increase initial receptor-activated ... of these calcium increases were accompanied by increased Ins(1,4,5)P3, indicating that calcium can enhance receptor-induced Ins(1,4,5)P3 increases The peak Ins(1,4,5)P3 increases were similar ... receptorinduced Ins(1,4,5)P3 increases in CHO cells expressing m1 mAChR PC12h cells express m1 mAChR and CCh-induced increases in calcium and investigate the cell-type dependence production, we assayed...

Ngày tải lên: 30/03/2014, 03:20

... fresh incubation buffer, but this time without creatine Creatine content within the cells was monitored at indicated time points to ascertain the creatine depletion rate To measure creatine content, ... arginine is additionally involved in several biosynthetic pathways that include a number of enzymes such as arginase (EC 3.5.3.1), arginine decarboxylase (EC 4.1.1.19) and nitric oxide synthase ... low in these cells Thus, we studied the uptake and release of creatine in EAC and S180 cells as a model system Figure shows that, if the cells were incubated for h in the uptake medium containing...

Ngày tải lên: 07/03/2014, 04:20

... explained by the fact that protein binding can increase the overall concentration of fatty acids present in an aqueous environment by effectively decreasing the insoluble fraction [2] Albumin, in ... [1-14C] AA during h After this period, the cells were incubated in the presence or in the absence of 8BrcAMP After this incubation, free AA was measured in the mitochondria, as described in the Experimental ... Journal compilation ª 2006 FEBS 5017 AA release in a specific compartment of the cells A F Castillo et al binding protein known also as diazepam-binding inhibitor (DBI) [37,38] It can be postulated...

Ngày tải lên: 16/03/2014, 12:20

... HeLa cells were grown in Eagle’s Minimum Essential medium (Nissui Pharmaceutical) containing 10% bovine serum in a CO2 incubator at 37 °C Screening for Hsp modulators A reporter plasmid containing ... examined the effects of SA on stress response in mammalian cells using a simple screening system, and revealed that SA is a potent Hsp inducer in mammalian cells, thereby protecting cells against ... into C3H10T1/2 cells (1 · 107 cells per 100 mm dish) with 30 lL lipofectAMINE reagent (Invitrogen) according to the manufacturer’s instructions, and incubated for 48 h Cells were then maintained...

Ngày tải lên: 17/03/2014, 10:20

... SDS-mini PAGE and Western blotting Indirect immunofluorence CV1 cells growing in coverslips were incubated at 37 °C or 43 °C for h as indicated in the text and figure legends They were then washed ... in Fig 6, hsc70 was found to bind to immobilized Mydj2-His protein (lanes 3, 3¢) In contrast, no binding between the immobilized dj2 protein and the inducible hsp70 protein was observed (Fig ... forms of Mydj2 protein by Western blotting analysis, using the same anti-dj2 Ig as in immunofluorence experiments As shown in Fig 5, Mydj2 does not seem to be heat-inducible, in CV1 cells at the times...

Ngày tải lên: 17/03/2014, 17:20

... Mouse brain tissue was used as a positive control The reduced amount of Glu-tubulin in CAD cells was not a result of a lack of (or inhibition of) the putative detyrosinating enzyme (tubulin carboxypeptidase) ... (B) Cells obtained as in (A) were subjected to western blotting and stained with the same antibodies as in (C) For staining with each antibody, identical volumes of CAD cell samples were run As ... corresponding to Tau in both differentiated and nondifferentiated CAD cells was similar to that in brain tissue (Fig 5B) LIS1 but not doublecortin is expressed in CAD cells Other proteins, such as a-Lis...

Ngày tải lên: 23/03/2014, 05:22

... DMEM-HG containing 1.7 mm insulin After 48 h, insulin was withdrawn from the culture medium and cells were maintained in DMEM-HG until use Measurement of the glucose concentration in the incubation ... [Ca2+]i-increasing agents and PDE1 inhibitor on glucose consumption in 3T3-L1 fibroblasts It has been shown that DIF-1 increases [Ca2+]c in mammalian cells [9–11,20] and that DIF-1 is a specific inhibitor ... consumption in a way other than by increasing [Ca2+]c or inhibiting PDE1 To support our hypothesis that an increase in [Ca2+]c is not involved in the DIF-1-promoted glucose consumption, we examined...

Ngày tải lên: 23/03/2014, 09:20

... the PMA-induced transcription of lysosomal acid lipase and acid sphingomyelinase in THP-1 cells In this study, we show that Sp1 is involved in PMA-induced expression of DNase II in HL-60 cells ... was significantly increased in PMA-treated cells, whereas that of the 95 kDa band was not altered It is possible that increased levels of the 105 kDa Sp1 contribute to the increased Sp1 binding ... protein levels and binding of Sp1 and Sp3 are increased in PMAtreated cells These findings indicate that Sp1 and Sp3 play a pivotal role in the transcriptional activation of DNase II in HL-60 cells...

Ngày tải lên: 23/03/2014, 17:21

... 4G ⁄ 5G insertion ⁄ deletion, which has stimulated increased interest in PAI-1 as a risk factor for venous thrombosis [25] In particular, the 4G allele in this polymorphism has been associated ... these signaling pathways lead to the induction of PAI-1 in endothelial cells Interestingly, PAI-1 upregulation by TGF-b has also been shown to involve ROS production in mesangial cells [30] Furthermore, ... incubation of endothelial cells with 100 lm H2O2 for 10 increased DCF fluorescence, which was then suppressed by cotreatment with NAC These findings indicate that both TNFa and H2O2 increased intracellular...

Ngày tải lên: 30/03/2014, 11:20

... has been found to be constitutively activated in 50-90% of all malignant tumors, including 53% of anaplastic astrocytomas and 53% of glioblastomas [3] In gliomas, cytokines, such as interleukin ... limitation of this assay is that an increase in the mean percentage of PBMCs displaying p-STAT-3 was not detected in all cases of malignant gliomas; however, an elevation in this value during follow-up ... Stat3 in IL-6-induced regulation of growth and differentiation in M1 leukemia cells EMBO J 1996, 15:3651-3658 Ghirnikar RS, Lee YL, Eng LF: Inflammation in traumatic brain injury: role of cytokines...

Ngày tải lên: 18/06/2014, 15:20

... used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning ... both lineages of influenza B These results indicate that influenza reverse genetic performed exclusively in MDCK cells can efficiently result in the rescue of seasonal FluMist vaccine strains as ... vaccine manufacture Although current production of influenza vaccines for human vaccination is in most cases an egg-base process, many vaccine manufacturers are actively developing cell-based influenza...

Ngày tải lên: 18/06/2014, 18:20

... used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning ... both lineages of influenza B These results indicate that influenza reverse genetic performed exclusively in MDCK cells can efficiently result in the rescue of seasonal FluMist vaccine strains as ... vaccine manufacture Although current production of influenza vaccines for human vaccination is in most cases an egg-base process, many vaccine manufacturers are actively developing cell-based influenza...

Ngày tải lên: 20/06/2014, 01:20

... gonadotropin; COC: cumulus-oocyte complex; PKA: protein kinase A; p38 MAPK: p38 mitogen-activated protein kinase; PI3K: phosphatidylinositol 3-kinase; ERK: extracellular-signal regulated protein; SRC: ... FSH in granulosa cells Mol Endocrinol 2000, 14:1283-1300 Qin L, Partridge NC: Stimulation of amphiregulin expression in osteoblastic cells by parathyroid hormone requires the protein kinase A ... oocyte meiotic resumption in porcine COCs as well as in mice Conclusion Herein, we showed that the expression of EGF-like factor and TACE/ADAM17 in cumulus cells was induced by a PKA-, p38 MAPK-...

Ngày tải lên: 20/06/2014, 07:20

... cificeps-non eht kcolb ot Co73 ta nim 001 rof )ASB/SBP( ASB %4 gniniatnoc SBP ni )ASU ,demyZ( mures taog %2 dna )ASU ,LRB-ocbiG( mures esroh %2 htiw detaert erew selpmas ehT tnemtaert hcae neewteb sesnir ... tneiciffus ecnO sCSM fo laitnetop cinegoetsO )ASU ,demyZ( 001 : detulid GgI esuom-itna CTIF :seidobitna yradnoces eht dna ,)ASU ,nocimehC( nitnemiv dna )ASU ,llecnA( 2-HS ,)ASU ,negnimrahP( 16/15DC seidobitna ... ytisned a ta )ASU ,cnuN( ksalf erutluc eussit 2mc 571 ot 01 a otni detalp yllaitini erew sllec ehT noitartnecnoc 2OC %5 a dna Co73 ta )ASU ,LRB-ocbiG( nicymotperts-nillicinep %3.0 dna ])ASU ,LRB-ocbiG(...

Ngày tải lên: 07/08/2014, 18:21

... which lasted for 24 hours Mast-Cell Activation N-Acetyl-␤-D-Hexosaminidase Release Assay Mast _cells to be used for ribonuclease (RNAse) protection assay and ␣-hexosaminidase release assay were ... mast cells, leading to degranulation as measured by ␤-hexosaminidase release (Figure 1A) When Fc⑀RI was coaggregated with Fc␥RIIB by the addition of RAM IgG, the release of ␤-hexosaminidase was inhibited ... protein kinase B by insulin and IGF-1 EMBO J 1996;15:6541–51 Coaggregation of Fc⑀RI with Fc␥RIIB in Mast Cells — Ekoff et al 97 33 Aman MJ, Lamkin TD, Okada H, et al The inositol phosphatase...

Ngày tải lên: 08/08/2014, 21:20

... further increasing the antibody’s depleting activity [7] Rituximab has been used in the treatment of B-cell non-Hodgkin’s lymphoma as a single agent as well as in combination therapy, emphasizing ... immunoglobulin levels are usually maintained, possibly as a consequence of plasma cells being spared B-cell depletion in antibody-mediated diseases It is understandable that rituximab has been most ... confirm that, at least in some patients, plasma cells are not sufficient to maintain autoantibody levels and that continuous B-cell recruitment and activation are necessary to maintain autoantibody...

Ngày tải lên: 09/08/2014, 01:21

... helicases and/or topoisomerases) in order to bind more avidly to the TLR9 and to initiate downstream signaling Interestingly, unprimed follicular B cells, in contrast to marginal zone (MZ)-B cells, ... immunoglobulin switch regions) These motifs can block TLR9-induced activation in both a cis and trans manner Surprisingly, chromatin-containing immune complexes in lupus sera were capable of inducing ... indispensable role in lupus pathogenesis Interestingly, IFN priming induces higher IL-6 and IL-10 secretion from CpGDNA stimulated MZ-B cells [110] Although MZ-B cells, at least in murine lupus, may...

Ngày tải lên: 09/08/2014, 07:20

... ionizing radiation, and can be repaired by homologous recombination (HR) or non-homologous end joining (NHEJ) in mammalian cells A number of essential proteins, including DNA-dependent protein kinase ... kinase (DNAPK), DNA-ligase IV, Rad50, and Artemis have been identified as regulators of NHEJ Ku proteins are a component of DNA-dependent protein kinase (DNA-PK), and are involved in the repairing ... 10% heat-inactivated calf serum (56°C for 30 min), penicillin (100 units/ml), and streptomycin (100 μg/ml) The cells were grown as a monolayer and maintained in the late exponential phase when...

Ngày tải lên: 09/08/2014, 09:21

... peroxidase and diaminobenzadine as the substrate and enhanced with avidin-biotin complex (Vector Laboratories, Burlingame, CA, USA) Mouse IgG was used as a negative control Immunopositivity was assessed ... medium (Invitrogen Corporation) supplemented with 10% FBS The J774 cell line (derived from murine macrophages, kindly donated by Robin May, University of Birmingham, UK) was maintained in DMEM/F-12 ... removing potential matrix-degrading enzymes, phagocytosis can trigger the release of antiinflammatory cytokines such as transforming growth factorbeta whilst inhibiting the production of proinflammatory...

Ngày tải lên: 09/08/2014, 10:23