... of whole and skim milk Partly demineralized WPC (baby food, special WPC products) Production of whey protein concentrates and isolates Defatting of whey for high protein WPC Standardization of ... separation and fractionation of fat globules, the reduction of bacteria and spores in skim milk, concentration of casein micelles (for cheese manufacturing), and purification of serum proteins, and j ... lactoferrin, transferrin, and some minor proteins and peptides (see also Table 2.2) For example, b-lactoglobulin can be used in emulsification, foaming and gelling [23], and for lactoferrin and...
Ngày tải lên: 02/04/2014, 15:04
... sub -complex Therefore, the presence of both Qcr9p and Bcs1p is required for the insertion of ISP into the bc1 sub -complex, but the presence of only one of these two subunits does not substitute for ... described previously [49,50] Standard procedures were used for the preparation and ligation of DNA fragments, for the transformation of Escherichia coli and for the isolation of plasmid DNA from bacterial ... the supercomplex formation These new findings open up several avenues of investigation and illustrate that a significant amount of work is still necessary for a complete understanding of the assembly...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity ppt
... at pH 4.7 of PpPr and S cerevisiae (saccharopepsin) by variant forms of IA3 from S castellii and S cerevisiae Sequences of IA3 from S castellii and S cerevisiae are depicted by open and dark-shaded ... Winterburn et al N-terminal extension of IA3 Table Construction of mutant forms of IA3 from S cerevisiae and S castellii The indicated pairs of forward (F) and reverse (R) oligonucleotide primers ... Denmark) for help with production of recombinant PpPr; to John Fox, Alta Biosciences, Birmingham, for provision and analysis of synthetic peptide variants of IA3; and to Doug Lamont and Kenny...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... segregation pattern, and some spores were prototrophic for both markers Haploid spores of VZ4, VZ6, VZ14 and VZ9 were then selected for Trp+ and Leu+, Trp+ and His+, His+ and Ura+, or His+ and Leu+ prototrophy, ... panels A and B and the relative amounts of each of the subunits determined by densitometry scanning of the stained Western blots of the q° and CKL57 membranes are shown in panels C and D, respectively ... several yeast bc1 complex mutants [31], the amounts of core and core proteins were significantly influenced by the absence of other subunits of the bc1 complex Deletion of the genes for subunit 7,...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Observation of a chaotic multioscillatory metabolic attractor by real-time monitoring of a yeast continuous culture doc
... mutual information I(k) between points in the time series separated by k time intervals, i.e between points i and i + k for all values of i The mutual information I(k) measures the amount of information ... each of the time windows, except that all PSD values > 0.3 have been mapped to red to enhance the contrast time and has a more complex waveform in the latter Fourier analysis of windows of the ... through the study of these organisms often leads to advances in our general understanding of eukaryotes We report here on the observation of chaotic oscillations in a continuous culture of the budding...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
... 1.25 mL of 1.5 m TrisHCl buffer, pH 8.8, and mL of water was boiled for and after cooling to room temperature, 1.65 mL of acrylamide solution (30%), 60 lL of 10% ammonium persulfate solution and ... of 0.01–0.5 mg mL)1 protein, were added to a suspension of 100 mg of raw corn starch in mL of 0.05 m sodium acetate at pH 5.6 and 4.5 for Glu and Glm, respectively, which are optimal values for ... 150, 151–152 Ramakrishnan C & Ramachandran GN (1965) Stereochemical criteria for polypeptide and protein chain conformations II Allowed conformations for a pair of peptide units Biophys J 5, 909–933...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
... features of proteins of the GH72 family and demonstrate that the most N-terminal highly conserved cysteine is crucial for the folding and maturation of Gas1p Materials and methods Strains and growth ... sequence of GAS1 Twenty-five cycles of a 45 s melting step at 94 °C, a annealing step at 50 °C for Gas1E161Q-H and 53 °C for Gas1E262Q-H, and a extension step at 72 °C were performed and the Taq ... addition of chase mixture, or after 10, 30 and 60 chase periods, mL of culture was withdrawn and further reactions were stopped by the addition of NaF and NaN3 to final concentrations of 10 mM...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane doc
... addition of mM EDTA and mM of NEM Mitochondrial membranes were then dissociated in the presence of 20 mM of NEM for SDS/gel electrophoresis and Western blot analysis For BN/PAGE analyses of crosslinked ... devoid of Cys28 (not shown), thus indicating the involvement of Cys28 in the formation of the adduct The 21.4-kDa band corresponded to a homodimer of subunit e resulting from the formation of a ... higher than that of the remaining dimeric form of the ATP synthase (Fig 6D) Owing to the destabilization of supramolecular ATP synthase forms of e19A mitochondria, a continuous band of subunit i...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf
... downstream of the DNA-binding domain of GAL4 For prey, a cDNA encoding GAPDH3-C97 inserted downstream of the activation domain of GAL4 was used Open boxes and solid lines indicate open reading frames and ... Biochem 269) 3563 and for TDH2, and and (Table 1) for TDH3, respectively, were used The PCR products were inserted into pUC19 vector, and the resulting plasmids, named pUCTDH1, pUCTDH2 and pUCTDH3, ... that ultradian oscillation of energy metabolism and coupling of oscillations of cell division and stress responses in continuous culture were disrupted by inactivation of the GTS1 gene [16] We...
Ngày tải lên: 31/03/2014, 21:21
cadaver dog handbook forensic training and tactics for the recovery of human remains
... demand for training and handling information, as well as for standardization and professionalization of training and handling methods The misapplication and incorrect handling of cadaver dogs has ... basics of understanding scent, canine olfaction, and the ABC’s of training and training equipment Chapters and are focused on the forensic context, including the demands of professionalism and the ... my standpoint as a canine trainer, this book will inform the novice of the complexity of the process To the working handler of cadaver dogs, you will better understand areas of training and working...
Ngày tải lên: 03/06/2014, 01:36
state university of new york press adorno the recovery of experience oct 2007
... structure and consequently ignore the kinetic force of a concept It is rather demanded that the force of the general concept be transformed into the self-development of the concrete object and that ... elimination of the cognitive significance of the subjective, and the formation of the constituting subject In fact, for Adorno, these two are one and the same development seen from different points of ... account of the operation of the understanding.12 The emphasis therefore shifts from philosophy of history to the analysis of how to resist, or work against the tendency of the habitual operation of...
Ngày tải lên: 11/06/2014, 12:47
differentiation of brewing yeast strains by pyrolysis
... thank Professor Douglas B Kell for use of PyMS and FT-IR E.M.T and R.G u are indebted to the Wellcome Trust for financial support (grant number 042615/Z/94/Z) D.E.Q is grateful to the Directors of ... spectrometry and artificial neural networks J Sci Food Agric 63, 297–307 Goodacre, R., Neal, M J and Kell, D B (1994a) Rapid and quantitative analysis of the pyrolysis mass spectra of complex binary and ... Haverkamp, J and Hileman, F D (1982) Pyrolysis Mass Spectrometry of Recent and Fossil Biomaterials Elsevier Morrison, K B and Sugget, A (1983) Yeast handling, petite mutants and lager Proceedings of the...
Ngày tải lên: 12/06/2014, 11:18
báo cáo hóa học: " Recovery of visual fields in brain-lesioned patients by reaction perimetry treatment" potx
... f VF border for form perception (100% correct discrimination of white circles and squares of equal size and luminance measured by kinetic perimetry Page 11 of 16 (page number not for citation ... analysis was performed to test for the influence of the type of treatment ocularity (monocular Page of 16 (page number not for citation purposes) Journal of NeuroEngineering and Rehabilitation ... delayed or no reactions (errors) See text for details treatment effects ourselves by using two types of perimetry (automatic static and kinetic) and other functions, in some of the out -of- town patients...
Ngày tải lên: 19/06/2014, 10:20
báo cáo hóa học: " Apolipoprotein E isoform-dependent dendritic recovery of hippocampal neurons following activation of innate immunity" pot
... isoform-specific neurotrophic actions in a variety of cell culture models The initial studies were performed using fetal rabbit dorsal root ganglion cultures and showed that, in the presence of ... #P < 0.01 for TR APOE2 vs control, P > 0.05 for TR APOE4 or TR APOE2+RAP vs control, and ^P < 0.05 for TR APOE2 vs TR APOE2+RAP Page of (page number not for citation purposes) Journal of Neuroinflammation ... LaserSharp software (BioRad) Five or more MAP2-stained neurons were randomly selected and dendrite length and branch number measured with Neurolucida SZM and CS were blinded to APOE of the astroctyes...
Ngày tải lên: 19/06/2014, 22:20
Báo cáo hóa học: " Recovery of divergent avian bornaviruses from cases of proventricular dilatation disease: Identification of a candidate etiologic agent" pptx
... Florida and participated in review of clinical and histological status of cases and controls included in the study, DG and JLD oversaw the overall conception and design of the project and supervised ... polymerase and dATP for 10 minutes at 72°C For each product, independent transformants were prepared for standard dideoxy sequencing on an ABI3730 sequencer (ElimBio, Hayward CA) Forward and reverse ... oligo(dT) and random hexamer primers, and then PCR-amplified using a modified random amplification strategy compatible with the Solexa sequencing platform (Materials and Methods) An initial set of 1.4...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo hóa học: " Blind recovery of k/n rate convolutional encoders in a noisy environment" pdf
... depicts Pdet compared with Pe, for 1, 5, and 50 iterations and for L = 200 For 1, 10, 40, and 50 0.8 0.8 Probability of detection Probability of detection Page of 0.6 0.4 Iteration Iteration ... Probability of detection, probability of falsealarm, and probability of miss compared with Pe For the C(3, 1, 4), the probability of detection, the probability of false-alarm, and the probability of miss ... Probability of detection, probability of falsealarm and, probability of miss compared with Pe For the C(2, 1, 7), the probability of detection, the probability of false-alarm, and the probability of miss...
Ngày tải lên: 20/06/2014, 22:20
Báo cáo hóa học: " Research Article Recovery of Myocardial Kinematic Function without the Time History of External Loads" ppt
... measures of accumulated errors: such as in frame #20, the maximum, mean and standard deviation of errors of KLLS are 0.114, 0.044, and 0.036; the maximum, mean and standard deviation of errors of KL ... Estimation of Myocardial Motion and Input Forces To handle unknown input forces in (10), we propose a framework of simultaneous estimation of myocardial kinematic function and input forces, which ... errors of the Kalman filter; (d) mean, (e) max, and (f) standard deviation errors of the Kalman filter and the least-square filter; all the x axis in the figures are the numbering of frame and the...
Ngày tải lên: 21/06/2014, 19:20
Báo cáo hóa học: " Research Article A Sharing-Based Fragile Watermarking Method for Authentication and Self-Recovery of Image Tampering" potx
... overhead and latency, which are determined by the parameters (N,K) of the RS code In the case of SR ARQ, the number of retransmissions of a packet is a random number between and Ntr Therefore, ... transmission of an L-bit packet involves L/(Nc log2 (M)) OFDM blocks Typically, the number Nc of carriers is on the order of 100 to 1000 Because of the large number of subcarriers, OFDM turns the ... effect of relaxing this assumption is briefly discussed in Section For x = 0, Ppack (x) and − Ppack (x) equal − 2−L and 2−L , respectively For x→∞, Ppack (x) and − Ppack (x) converge to zero and...
Ngày tải lên: 21/06/2014, 22:20
Báo cáo hóa học: " Research Article A Simplified Constant Modulus Algorithm for Blind Recovery of MIMO QAM and PSK Signals: A Criterion with Convergence Analysis" ppt
... opposed to CMA The major advantage of SCMA is its low complexity compared to that of CMA and MMA Because, instead of using both real and imaginary parts as in CMA and MMA, only one dimension, the ... (n) + (1 − λ)zR, (n)zI,m (n) (70) 6.2 Complexity We consider the computational complexity of (66) for one iteration and for all equalizer outputs With (i) for CC-SCMA, e (n) = zR, (n)2 − R zR, ... behavior So, in the case of QAM signals, it is preferable to use a modified cross-correlation term because of its low complexity and of its similar performances compared to the one of the classical cross-correlation...
Ngày tải lên: 22/06/2014, 06:20
Báo cáo nghiên cứu khoa học: "USE OF IMMOBILIZED YEAST CELL IN ALCOHOL FERMENTATION FROM MOLASSES" ppsx
... mass of free yeast and Alco3.0% Fermentative opt pH and opt t0 of free yeast and Alco3.0% are similar that are pH = and t = 300C 3.5.Determination of optimal fermentable time of free yeast and ... 1.5381 and b is 6.0895 Using the slope and intercept function of Microsoft Excel to determine a and b, and RSQ function determines R2 = 0.9866 Note: The test samples were diluted by 103 Table 3.2 and ... Department of Chemical Engineering University of Maryland, (2003) [8] Nam Sun Wang Measurements of Cell Biomass Concentration Department of Chemical Engineering University of Maryland, (2003)...
Ngày tải lên: 22/07/2014, 03:20