... enthalpies of binding and stoichiometries were determined from the binding isotherm by fitting a : binding model to the data using the software provided by the manufacturer Results Reaction of fosfomycin ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins However, two other positively charged amino ... replacement of R120 by lysine reduces the probability of forming the fully closed form of the protein The results obtained with the K22V/R120K double mutant protein point toward a central role of arginine...
Ngày tải lên: 19/02/2014, 13:20
... The motif present in DptF is GLDSV, indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) or leucine (L) in the conserved sequence To determine ... carrier proteins (ACPs) [14,15] The genes dptE and dptF are localized immediately upstream of the NRPSs of A21987C The resulting proteins DptE and DptF were predicted to be involved in the lipidation ... Assay Fig In vitro phosphopantetheinylation of apo-DptF and apoAcpK Coomassie blue-stained SDS–PAGE gel (left) and in- gel fluorescence (right) of the fluoresceinyl-ACP (+) indicates the reaction...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx
... advantage of the trypsin-sensitive region situated in the linker domain of Abcb1a Following trapping in the absence or presence of Vi and mild-trypsin treatment, the trypsin degradation products of the ... in the glutamine (length of the side chain maintained) and asparagine (shorter side chain) mutants, then trapping in the absence of Vi now occurs [39] (Fig 3); this is also the case when the ... Another notable feature of the single-site IC mutants (including the glutamine substitutions) [39] is the fact that, in the absence of Vi (± drugs) labeling of the enzymes with the mutation in...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: "Some Comments on Algorithm and Grammar in the Automatic Parsing of Natural Languages" ppt
... view the executive routine of the algorithm which contains the grammar as the actual parsing algorithm and to view the remaining portions as forms of input data Leaving aside the matters of rule ... of structuring the parsing program as an executive main routine with appropriate subroutines This raises the further question of the functions and design of the executive routine and subroutines ... difference in the adequacy of the parsings of natural-language sentences will be alterations of the format itself in terms of increasing the degree of context-sensitivity This in effect means that the...
Ngày tải lên: 07/03/2014, 18:20
ANTIBIOTIC RESISTANCE OF ESCHERICHIA COLI ISOLATED FORM POULTRY WORKERS, PATIENTS AND CHICKEN IN THE EASTERN PROVINCE OF SAUDI ARABIA ppt
... Ampicillin, gentamicin Ampicillin, spectinomycin Ampicillin, tetracycline Ampicillin, TMPϩSMX Ampicillin, gentamicin, tetracycline & TMPϩSMX Ampicillin, gentamicin, spectinomycin, tetracycline & TMPϩSMX ... at King Fahd Hospital of the University and the College of Medicine for their assistance We also thank Prof A Ghandour, Department of Microbiology, King Faisal University, for reviewing the manuscript ... source of pathogenic organisms in humans, we concur with the calls to ban the use of antibiotics as growth promoters and recommend the restriction of their use to treat infections in the poultry industry...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx
... processing of APP in these cells In summary, the results indicate that PKCa and PKCe have differential roles in the regulation of APP processing and sAPPa release in SH-SY5Y cells – the former being ... data in the literature that indicate PKCe as the only protein kinase isoform involved in the signalling pathway downstream of muscarinic m3 receptors in SK-N-BE(C) neuroblastoma cells [15] The ... resulted in the inhibition of phorbol ester-induced sAPPa release [14] It is worth mentioning that the involvement of PKCa in these experiments has been ruled out because of the lack of inhibition...
Ngày tải lên: 23/03/2014, 13:20
EQUALITY and LIBERTY in the Golden Age of State Constitutional Law ppt
... found in the state constitution, the principle of equality is an integral part of the state’s constitutional law, inherent in the due process clause of the West Virginia Bill of Rights.23 And the ... remained subject to the minimalism of rationality review Thus, the in exibility within the minimal tier of scrutiny persisted Finally, this moved the Court in a few cases to invest minimal scrutiny ... nature of the classification in question, the constitutional and societal importance of the individual interest adversely affected, and the strength of the state interest asserted in support of the...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: The role of ADAM10 and ADAM17 in the ectodomain shedding of angiotensin converting enzyme and the amyloid precursor protein ppt
... ADAM10 and TACE are involved in the shedding of APP, neither ADAM is involved in the shedding of ACE Furthermore we show that APMA can distinguish between the shedding of ACE and APP Materials and ... expressing ACE were transfected with the ASOs and then exposed to APMA and the levels of soluble ACE in the medium examined (Fig 5) APMA caused a twofold increase in the shedding of ACE from the ... Effect of APMA on the shedding of APP and ACE from CHO cells CHO cells stably expressing ACE were incubated in Opti-MEM in either the absence or presence of the indicated amount of APMA for 30 The...
Ngày tải lên: 30/03/2014, 14:20
Workshop Training Manual: " Technologies for improving goat housing and hygiene in the central provinces of Vietnam " ppt
... Highlands, 8.9% for the Central Coast, and 2.1-3% for the Southeastern and the Southwestern) Goats in the Northern moutainous areas make up 48% of the country, and 67% of the North Most of them ... another, unlike the destructive gobbling nature of the cows In the other hand, goat helps to prevent the weeds and fertilize the plants with its excrement • Lack of experience is one of the main ... contamination and plague spreading still lingers around 3) Help the goats regain their strength by sufficient feeding and mineral and vitamine supplement 4) In case of diarrhoea, drinking water and salt...
Ngày tải lên: 21/06/2014, 06:20
the rootkit arsenal escape and evasion in the dark corners of the system
... Assume the Worst-Case For the sake of keeping things interesting: Below Gotham Laboratories Assumption Let’s assume we’re facing off against a highly skilled, well-armed, adversary The “they’re all idiots” mentality is dangerous (don’t underestimate your opponent!) ... Tolerance The Tradeoff Minimize Footprint → Sacrifice Restart Survival May need to balance the two based on: The type of environment being targeted The value of the data to be acquired The skill ... Laboratories The FILE_BASIC_INFORMATION argument stores four LARGE_INTEGER values These values represent the number of 100-nanosecond intervals since 1601 When these values are small, the Windows API doesn’t translate them correctly ...
Ngày tải lên: 06/07/2014, 15:32
Báo cáo lâm nghiệp: "Effects of defoliation on the frost hardiness and the concentrations of soluble sugars and cyclitols in the bark tissue of pedunculate oak (Quercus robur L.)" potx
... occurring in consecutive years – reduces the frost hardiness of the bark in the following winter via a reduction in the content of soluble sugars and/ or cyclitols To obtain preliminary indications ... significance of the increase in R2 by including additional variables into the model were tested using the distribution of F values [37] RESULTS 3.1 Growth increment, freezing injury and frost hardiness In ... defoliation) The freezing injury was determined as the slope b of the linear relationships between the log values of relative conductivity (RC; %) of the incubation solution and the duration of bark...
Ngày tải lên: 08/08/2014, 01:21
Báo cáo y học: "Small ruminant feed systems: perceptions and practices in the transitional zone of Ghan" docx
... participated in the design of the study and SD carried out the field study and identified some of the species All authors contributed to the compilation of the manuscript Competing interests The authors ... through both tethering and zero grazing and by scavenging, and obtained from public lands, other people’s private lands, and farmers’ own home gardens, in all seasons Ficus umbellata and banana are ... providing accommodation for the lead researcher Sincere thanks also go to the elders and farmers of the study area for their support and co-operation during the study The help of Mr Obrien Nyarko and...
Ngày tải lên: 10/08/2014, 09:21
báo cáo khoa học: "Instrument development, data collection, and characteristics of practices, staff, and measures in the Improving Quality of Care in Diabetes (iQuaD) Study" pptx
... the clinical management behaviours of individual clinicians and the organisations they work in In the United Kingdom, patients are managed by the integrated activities of clinical and non-clinical ... review of the use of theory in the design of guideline dissemination and implementation strategies and interpretation of the results of rigorous evaluations Implementation Science 2010, 5(14) Godin ... positively inclined towards performing the behaviours These two groups of measures will together form a large part of our explanatory variables in explaining variation in rates of performing the behaviours...
Ngày tải lên: 10/08/2014, 10:23
báo cáo khoa học: " Overweight in the Pacific: links between foreign dependence, global food trade, and obesity in the Federated States of Micronesia" ppt
... occupied the islands and soon became the "administering authority" of the U.N Trust Territories of the Pacific Islands (i.e Micronesia) The Federated States of Micronesia (FSM) did not become an independent ... Overweight and obesity prevalence in Kosrae, FSM in 1988 Overweight and obesity prevalence in Kosrae, FSM in 1988 and 2000 and the other states in the FSM became more Westernized during the U.S ... from the U.S were being consumed in large quantities, and these dietary changes led to dramatically increased prevalence of overweight and obesity The following section examines the role of the...
Ngày tải lên: 11/08/2014, 18:20
Báo cáo y học: "Functional constraint and small insertions and deletions in the ENCODE regions of the human genome" ppsx
... identify indels by requiring the inserted/deleted bases and the flanking five bases on either side of the indel to exceed a minimum Phred quality score of 22 If these minima were not met, then the indel ... results in Tables to are presented in two ways: as numbers of indel events (indels per 100 kb) and total indel length (indel bp per 100 kb) In the interests of brevity, indel rates are referred to in ... replication There are two potential limitations of the present study The first relates to the completeness and accuracy of the indel and genomic annotation data [19], ensuring which is a continuing exercise...
Ngày tải lên: 14/08/2014, 08:20
das - traders, guns & money; knowns and unknowns in the dazzling world of derivatives (2006)
... which they had no control They needed divine intervention to save themselves from their own folly In the end, the miracle was not forthcoming Beginning of the end/end of the beginning In July ... is the record of my time in the derivatives industry It is a collection of tales about the products, the people and the strange goings-on in the business Ordinary men and women not trouble themselves ... foreign businesses There were, of course, ‘problems’ The sudden increase in the rate of growth and demand set off rapid price rises The office space in Mumbai’s Narriman Point business district...
Ngày tải lên: 01/11/2014, 19:13
Ecological concern and sustainability in the selected works of al gore (sum)
... hands consensus and efforts to find the solution and agreed to take back the inherent beauty of the Earth and the ability to maintain or re-create the life of existing species and in the future 13 ... presents the key issues in other words, is the main engine of the present study DEFINITION OF TERMS This section provides definitions of the terms used in the study, in order to help the readers ... requirement of their workplace are the basic elements These suggestions can evoke, and guide them to the concerns about the environment and its sustainability Since then, the application of the principles...
Ngày tải lên: 04/12/2014, 01:09
Ecological concern and sustainability in the selected works of al gore
... organizing meaning; creating theory from emerging themes; writing an understanding; and drawing conclusions The essential features in the treatment of materials were considered by the researcher in ... demands and economic demands; the literary devices used by Gore in unveiling the theme of ecological concern and sustainability; and the literary approaches prevalent in the treatment of the ... keeping countries such as Britain warmer than expected The global warming effects on the Indian subcontinent vary from the submergence of low-lying islands and coastal lands to the melting of...
Ngày tải lên: 04/12/2014, 01:09
The Development Continuum Change and Modernity in the Gayo Highlands of Sumatra, Indonesia
... now deep in the Alas Valley in the Gayo Highlands I was on my way to the village of Aih Nuso located in the northern part of the plush Alas Valley along the banks of the Alas River in the Gayo ... source of protein or income depending on the current financial situation of the family Most of the subsistence needs of the people of Aih Nuso come from the surrounding rivers and forests with the ... grateful to my thesis committee, Profs Ammarell, Collins, and Duschinski, for their support, encouragement, and patience in reading drafts of the chapters and offering theoretical and practical...
Ngày tải lên: 14/05/2015, 10:07