detection of hif 1 mrna by northern blotting

... lamina propria J Immunol 19 92, 14 9:2 816 -2822 Page 10 of 11 (page number not for citation purposes) AIDS Research and Therapy 2009, 6:20 10 11 12 13 14 15 16 17 18 19 20 21 Brenchley JM, Schacker TW, ... XX186 9/24/2008 N/A 839 XX148 9 /17 /2008 N/A 724 XX0 21 8 /19 /2008 N/A 845 XX280 8/26/2008 10 03 923 XX495 8 /16 /2008 354 71 238 XX326 8/23/2008 2 014 9 392 XX286 9 /17 /2008 2974 320 XX 119 8/26/2008 18 2 31 ... HIV -1 Negative N = 10 Sample Date Viral Load (copies/ml) CD4/mm3 642 N/A 13 17 XX163 8 /19 /2008 N/A 899 XX983 8 /19 /2008 N/A 11 53 XX2 71 9/5/2008 N/A 828 XX003 9 /16 /2008 N/A 547 XX744 9/25/2008 N/A 15 20...

Ngày tải lên: 10/08/2014, 05:21

... the effect of C 119 9-Tiam1 In addition, the effects of MEK1,2 inhibitors and catalase on C 119 9Tiam1-induced TIMP -1 secretion in quantitative TIMP -1 immunoassays were verified by immunoblotting (data ... Miki T & Gutkind JS (19 95) The small GTP- 4766 16 17 18 19 20 21 22 23 binding proteins Rac1 and Cdc42 regulate the activity of the JNK ⁄ SAPK signaling pathway Cell 81, 11 37– 11 46 Bagrodia S, Taylor ... overexpression of C 119 9-Tiam1 strongly activated Rac, but neither C 119 9-Tiam1 nor V12-Rac1 affected Rho activity (Fig 3D) This suggests that Rac-induced upregulation of TIMP -1 is neither 4758 mediated by...

Ngày tải lên: 19/02/2014, 05:20

... { t~-~-=, ~=-~ } \ e 0.7 0.6 o 0.5 0,4 0.3 % o.2 LR0 LR1 10 62 11 04 11 20 11 34 10 07 9 21 760 811 799 760 697 695 844 6 71 667 622 424 9 21 319 788 469 665 255 397 homophone word to another homophone ... number of problems detected as errors by DL1 is given by Eq.4, and the number of real errors in these detections is TGpg Therefore, the precision P1 of DL1 is as follows: p1 = Pg by a is 1, 6 31, and ... the recall of DL1 Because a of DL1 is the same as that of DL0, the number of problems detected as errors by a is given by Eq.4 In the case of DL1, problems judged by ~ of DL0 are judged by the written...

Ngày tải lên: 22/02/2014, 03:20

... proteasome inhibitor, MG132 used in the concentration range 0 10 00 nm to prevent degradation of IjBa In the presence of MG132, TNFa-induced expression of PAI -1 detected at the level of mRNA (Fig 5C) or ... then treated with TNFa (50 ngÆmL )1) or H2O2 (10 0 lM) (A) Expression of PAI -1 mRNA determined by real-time PCR and (B) activity of PAI -1 promoter (C) Treatment of endothelial cells with the oligonucleotide ... cells Thromb Res 77, 11 9 13 1 Samad F, Yamamoto K & Loskutoff DJ (19 96) Distribution and regulation of plasminogen activator inhibitor -1 in murine adipose tissue in vivo Induction by tumor necrosis...

Ngày tải lên: 30/03/2014, 11:20

... (y|Xy = 1) We now show that P (y|Xy = 1) = P (y)/P (Xy = 1) , and that this expression is equivalent to (8) P (y|Xy = 1) = P (y, Xy = 1) P (Xy = 1) (17 ) Recall that P (y, Xy = 0) = because of the ... receives all of the probability mass because it appears in all of the contexts, even though it is n k =1 P (X1 k ) |G| × 1( xk ∈ G) , (24) where P (X1 k ) is the precision of the first k items and 1( xk ... certainly be a factor, we raise 702 x 10 -0.5 Log probability Log probability -0.5 -1 -1. 5 -2 -2.5 x 10 -1 -1. 5 -2 Iterations -2.5 10 (a) Data log likelihood Iterations 10 (b) Log classification certainty...

Ngày tải lên: 31/03/2014, 20:20

... reaction carried out using 10 -fold serial dilutions of standard SBV DNA (1. 0 × 10 4 copies/µL): 1: 1. 0 × 10 0, 2: 1. 0 × 10 1, 3: 1. 0 × 10 2, 4: 1. 0 × 10 3, and 5: 1. 0 × 10 4 copies/µL, respectively ... inner (F1c + F2) Backward inner(B1c + B2) Loop Forward Loop Backward 5′pos 519 707 592 548 619 679 569 6 41 3′pos 539 724 616 568 640 700 5 91 663 Length 21- nt 18 -nt 46-mer(F1c:25-nt, F2: 21- nt) 44-mer ... between 1. 0 × 10 2 copies/µl to 1. 0 × 10 4 copies/µl of standard template RNA However, this was not observed in samples with between 1. 0 × 10 0 to 1. 0 × 10 1 copies/µl In Figure 3B, after adding µl of...

Ngày tải lên: 21/06/2014, 19:20

... 26 81. 768 2678.836 2676.459 81 96 11 7 13 4 16 4 19 5 224 2 51 296 26 71. 772 2668.262 2663.669 2659.030 2654.393 2646.078 2640.364 2636.650 2634.700 T.T.N Lien et al / Talanta 80 (2 010 ) 11 64 11 69 11 67 ... 2525.0 17 17.5 767.5 457.5 347.5 11 9.5 95.7 71. 2 71. 2 70.0 67.0 61. 2 60.0 69.5 75.0 75.0 0.2 0.35 0.76 0.63 0.5 2.4 3 .14 3.00 1. 10 1. 64 1. 54 1. 53 1. 43 0.82 0.56 0.60 2.6 3.5 2.6 3.2 2.2 2.0 3 .1 2.6 ... Frequency (kHz) 13 17 21 25 28 32 36 2803.620 2798.995 2794.660 2790.5 31 2787. 518 2784 .14 5 2780.4 31 2776.974 27 71. 526 2746.940 39 43 46 49 52 56 59 62 65 70 2728.606 2 711 . 519 2706.566 2699. 414 2693.783...

Ngày tải lên: 02/07/2014, 14:14

... Circ Res 20 01, 88 :12 47 -12 53 44 Jewell UR, Kvietikova I, Scheid A, Bauer C, Wenger RH, Gassmann M: Induction of HIF- 1a in response to hypoxia is instantaneous FASEB J 20 01, 15 :13 12 -13 14 R 914 ... face of catabolic changes in articular cartilage Many molecular aspects of HIF- 1 signaling should be also studied to further clarify the role of HIF- 1 in the pathogenesis of OA References 10 11 12 ... Rheum 2002, 46:2 911 -2 916 Pfander D, Cramer T, Schipani E, Johnson RS: HIF- 1alpha controls extracellular matrix synthesis by epiphyseal chondrocytes J Cell Sci 2003, 11 6 :18 19 -18 26 Hall JL, Matter...

Ngày tải lên: 09/08/2014, 06:23

Ngày tải lên: 09/08/2014, 07:20

... 10 11 12 13 14 15 Yudoh K, Nakamura H, Masuko-Hongo K, Kato T, Nishioka K: Catabolic stress induces expression of hypoxia-inducible factor (HIF) -1a in articular chondrocytes: involvement of HIF1 a ... Arthritis Rheum 20 01, 44 :10 82 -10 88 Pfander D, Cramer T, Schipani E, Johnson RS: HIF- 1alpha controls extracellular matrix synthesis by epiphyseal chondrocytes J Cell Sci 2003, 11 6 :18 19 -18 26 Lin C, McGough ... reported that mechanical overload of bovine cartilage discs leads to an increased expression of VEGF-A via HIF- 1 In conclusion, the α-subunit of HIF- 1 is stabilized and HIF- 1 activity is significantly...

Ngày tải lên: 09/08/2014, 07:20

... Rheumatol 19 93, 20 :11 62 -11 66 Wallace SL, Robinson H, Masi AT, Decker JL, McCarty DJ, Yu TF: Preliminary criteria for the classification of the acute arthritis of primary gout Arthritis Rheum 19 77, ... production of tumor necrosis factor alpha from human blood monocytes and synovial cells Cytokine mRNA and protein kinetics, and cellular distribution J Clin Invest 19 91, 87 :13 75 -13 81 Malawista ... Arthritis Rheum 19 85, 28 :10 39 -10 46 Di Giovine FS, Malawista SE, Nuki G, Duff GW: Interleukin (IL 1) as a mediator of crystal arthritis Stimulation of T cell and synovial fibroblast mitogenesis by urate...

Ngày tải lên: 09/08/2014, 10:20

... Genome Biology 2009, 10 :R 113 http://genomebiology.com/2009 /10 /10 /R 113 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Genome Biology 2009, tion Genes Dev 19 89, 3: 612 - 619 Perini G, Diolaiti ... Biology 2009, 10 :R 113 http://genomebiology.com/2009 /10 /10 /R 113 U87 10 27 766 209 475 205 14 7 967 97 HepG2 3 01 1285 246 MB2 31 276 14 6 HepG2 3 51 MB2 31 GAPDH MLL5 PPME1 (d) 10 00.0 p =1. 1e- 41 100% 20 47 ... 0 .15 All 15 0.00 0 .1 0.04 0.2 0.08 0.3 All HIF1 -bound 0.0 0.00 10 Density 0 .10 0.05 0.20 0 .10 0.00 Density 0.30 All HIF1 -bound Absent HepG2 HepG2 U87 mRNA (log2 scale) HIF1 bound mRNA 10 All HIF1 bound...

Ngày tải lên: 09/08/2014, 20:20

... by Sigma-Proligo, Singapore Table Primers and probe used in this study Name Sequencea Nucleotideb Sense 5’-TTGGTTACCATGCAAACAAYT-3’ 91- 111 Antisense 5’-TRTCTTGGGCRTGTGTAACA-3 15 2 -17 1 Probe 11 9 -14 3 ... virus in eastern Asia Nature 2004, 430(6996):209- 213 WHO: Evolution of H5N1 avian influenza viruses in Asia Emerg Infect Dis 2005, 11 (10 ) :15 15 -15 21 Smith GJ, Naipospos TS, Nguyen TD, de Jong MD, ... TL, Lim WW: Influenza A H5N1 detection Emerg Infect Dis 2005, 11 (8) :13 03 -13 05 18 Lu YY, Yan JY, Feng Y, Xu CP, Shi W, Mao HY: Rapid detection of H5 avian influenza virus by TaqMan-MGB real-time...

Ngày tải lên: 12/08/2014, 04:21

... Respir Res 2 010 , 11 :7 Page 14 of 14 56 Wenzel S, Holgate ST: The mouse trap: It still yields few answers in asthma Am J Respir Crit Care Med 2006, 17 4 :11 73 -11 76, discussion 11 7 611 78 57 Shapiro ... concentration (Spearman r = 0.6 219 ; P = 0. 013 , n = 11 independent exposure dates) North et al Respiratory Research 2 011 , 12 :19 http://respiratory-research.com/content /12 /1/ 19 Page of 14 Figure Bronchoalveolar ... Perspect 2009, 11 7 :19 19 -19 24 Page 13 of 14 14 Ricciardolo FL, Sterk PJ, Gaston B, Folkerts G: Nitric oxide in health and disease of the respiratory system Physiol Rev 2004, 84:7 31- 765 15 Maarsingh...

Ngày tải lên: 12/08/2014, 13:22

... http://www.retrovirology.com/content/6 /1/ 26 Scr Retrovirology 2009, 6:26 13 14 b) 16 11 ,4 HIV -1 expression 14 10 ,9 12 6,9 10 6,3 6,2 5,06 1, 0 0,3 Scr RCK/p54 LSm -1 GW182 XRN1 DGCR8 Drosha CDK9 siRNA Figure ... 6:26 tubulin 15 00 10 00 500 14 00 Hdm2 mRNA 12 00 10 00 800 600 400 200 Fractions 13 17 21 25 Figure RCK/p54 restricts HIV -1 mRNA association with polysomes RCK/p54 restricts HIV -1 mRNA association ... References 10 11 12 13 14 15 16 17 18 19 20 21 Brodersen P, Sakvarelidze-Achard L, Bruun-Rasmussen M, Dunoyer P, Yamamoto YY, Sieburth L, Voinnet O: Widespread translational inhibition by plant...

Ngày tải lên: 12/08/2014, 23:20

... coactivator NCOA3 - 6.53 212 154_at 212 157_at 212 158_at syndecan (heparan sulfate proteoglycan) SDC2 - 3.05 - 20.23 - 16 .54 212 463_at CD59 antigen p18-20 CD59 -3 .11 213 093_at 215 195_at protein kinase ... induced by HIV -1 Aids 19 98, 12 :563-570 10 Yin J, Chen MF, Finkel TH: Differential gene expression during HIV -1 infection analyzed by suppression subtractive hybridization Aids 2004, 18 :587-596 11 Cicala ... specifically in brain tumors Gene 19 95, 15 9 :13 1 -13 5 Page of 10 19 Rich T, Chen P, Furman F, Huynh N, Israel MA: RTVP -1, a novel human gene with sequence similarity to genes of diverse species, is expressed...

Ngày tải lên: 12/08/2014, 23:23

... 0.02 0 10 15 Fraction number 20 25 b Sam68∆C Sam68∆C +EDTA 11 13 15 17 19 21 11 13 15 17 19 21 Anti-myc c % of total actin mRNA Anti-L26 0.6 0.5 0.4 pcDNA +EDTA Sam68∆C 0.3 0.2 0 .1 0 10 15 20 ... Mechanism and regulation of mRNA polyadenylation Genes Dev 19 97, 11 ( 21) :2755-2766 Salles FJ, Richards WG, Strickland S: Assaying the polyadenylation state of mRNAs Methods 19 99, 17 (1) :38-45 Mangus DA, ... Studies were supported by an operating grant from CIHR M McLaren cloned Sam68Δ28ΔC Antibodies were generously provided by N Sonenberg, and H Schaal References 10 11 12 13 14 15 16 Schwartz S, Felber...

Ngày tải lên: 13/08/2014, 05:21

... Leukemia 2005, 19 :11 75 -11 83 Gilli SC, Salles TS, Saad ST: Regulation of the GATA3 promoter by human T-cell lymphotropic virus type I Tax protein J Cell Biochem 2004, 93 :11 78 -11 87 Popovic M, Sarin ... eo ph βP r .1 n 0 ,1 WT 0 ,15 Sup T1 Ta x 0,2 ph βP r .1 ne o B Relative Galectin -1 mRNA expression phβPr .1 neo NF-κB CREB SRF - Galectin -1 0,05 β-Actin phΒ Tax703 Tax TaxM22 TaxWT Figure of Analysis ... http://www.retrovirology.com/content/5 /1/ 105 B 10 0 10 0 ,1 NF-κB-Luc κ HTLV-Luc 350 * 300 250 ** 200 15 0 10 0 ** ** 50 ** CEM-T4 Jurkat E6 .1 phβPr.1neo SRE-Luc Tax M22 Sup T1 Tax WT D Normalized luciferase...

Ngày tải lên: 13/08/2014, 05:21

... therapy 10 11 12 13 14 Competing interests This study was partially supported by Cyclacel Pharmaceuticals, Inc 15 Authors' contributions 16 SB was responsible for execution of the HIV -1 infectivity ... mutations in HIV -1 Top HIV Med 2003, 11 (3):92-96 Tang JW, Pillay D: Transmission of HIV -1 drug resistance J Clin Virol 2004, 30 (1) :1- 10 Menendez-Arias L: Molecular basis of fidelity of DNA synthesis ... speckle regions J Cell Sci 20 01, 11 4(Pt 8) :14 91- 1503 Reed-Inderbitzin E, Maury W: Cellular specificity of HIV -1 replication can be controlled by LTR sequences Virology 2003, 314 (2):680-695 Chesebro...

Ngày tải lên: 13/08/2014, 05:22

... kon 10 5 M -1 s -1 sIN sIN no Mg koff 10 -3 s -1 Kd (nM) kon 10 5 M -1 s -1 koff 10 -3 s -1 Kd (nM) kon 10 5 M -1 s -1 koff 10 -3 s -1 Kd (nM) 1. 7 ± 0 .1 1.9 ± 0.2 1. 0 ± 0 .1 2 .1 ± 0 .1 5.9 ± 0.7 11 .1 ± 1. 3 1. 1 ... ± 1. 2 14 .0 ± 3.4 ND 1. 1 ± 0 .1 1 .1 ± 0.2 1. 1 ± 0.2 0.8 ± 0 .1 1.0 ± 0 .1 0.9 ± 0 .1 0.9 ± 0 .1 0.8 ± 0.2 - 1. 3 ± 0 .1 1.6 ± 0.2 1. 2 ± 0.2 0.6 ± 0 .1 0.3 ± 0 .1 0.3 ± 0 .1 1.0 ± 0.2 0.9 ± 0 .1 - 11 .8 ± 1. 4 ... 1. 2 ± 0.2 0.9 ± 0 .1 0.8 ± 0 .1 1.9 ± 0.2 0.5 ± 0 .1 - 1. 0 ± 0 .1 1 .1 ± 0 .1 0.9 ± 0 .1 1.0 ± 0 .1 0.2 ± 0 .1 0.2 ± 0 .1 1.2 ± 0.2 0.7 ± 0 .1 - 5.9 ± 0.7 5.2 ± 0.7 6.9 ± 1. 3 8.3 ± 1. 6 2.2 ± 1. 1 2.5 ± 1. 3...

Ngày tải lên: 13/08/2014, 09:20