... teacher is the monitor and the facilitator.Every member inthe group has to take part in Every member inthe group has to take part in their work going to each of the family in their their work ... going to each of the family in their neighbour to take the notes of the amount of neighbour to take the notes of the amount of energy they spent (water, electricity, ,oil,coal…) energy they ... to do and their All groups present their ways to do and their charts in front of the class and share the best charts in front of the class and share the best way to the web site of the resources...
... facilitator.Every member inthe group has to take part in Every member inthe group has to take part in their work going to each of the family in their their work going to each of the family in their neighbour ... describing information from the chart.•By the end of the lesson Ss.will be able to read the information from the chart and write a description from it.•This design can help them to infinite the ... do and their charts in front of the class and share the best charts in front of the class and share the best way to the web site of the resources file of City way to the web site of the resources...
... agonist-inducedalterations in BRET signal intensities reflecting 7TM dimerrearrangement as a result of the activating twist inthe ATDsof the CaR homodimer. Further investigations into the signal transference ... of CaR in living cells (Eur. J. Biochem. 269) 5077Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescenceresonance energytransfer ... FEBS 2002Agonist-induced rearrangement of the 7TMs in the CaR homodimer?One of the goals of the present study was to investigate,whether the activating twist inthe ATD dimer of the familyC GPCR...
... for the harnessing of wind energy, another renewable energy source (Cunanan, 2002). Asean countries lie mainly inthe Southeast Asian region and span the equator. Since they are all under the ... clear that farming can continue after the wind farm has been built. The usual sites for wind farms are in mountainous areas with low vegetation. In these areas, the land is used mainly as pasture ... continue unhindered after the wind farm has been built.(ref : Greece paper) Besides this, the leasing of land for wind turbines, can bring substantial benefits to landowners inthe form of increased...
... In [12], [13] this was achieved by translating the origin in the integrand of (1.6) to an arbitrary point y, and averaging against the L1massdensity |u(y)|2dy. In particular, the following ... Proposition 4.5. The argument here is similar to the induc-tion on energy arguments in [4]. The point is that the linear evolution of the 23Note added in proof: a bound of M(E) ≤ C ↑ (CEC) inthe radial ... finite for all E. In the argument of Bourgain [4] (see also [5]), the finiteness of M (E )in the spherically symmetric case is obtained by an induction on theenergy E;indeed a bound of the formM(E)...
... indicates that induction of FRET does not sim-ply depend on the length of linkers. Inthe linker con-taining seven amino acids, a Pro-Pro sequence that isnot contained in other linkers is incorporated. ... upon the distance between and relative ori-entation of two fluorophores, being independent of the strength of protein–protein interactions. Inthe case ofvery weak interactions as described in ... technique for the observation of protein–proteininteractions [14,15]. This method can be applied tointeractions in loose complexes in living cells, and hasan advantage in that the FRET strength...
... the latter. Interestingly, it appears that proteinbinding to ssDNA inthe absence of any nucleotidecofactor results in attenuation of spontaneous anneal-ing (compare line 1, Fig. 3 with line ... intensity of fluorescein as evidenced by the set where OsDmc1 was omitted inthe sample(Fig. 4B, line 1). In order to confirm that the increase in the fluorescence signal is specific to the emissionmaximum ... ATPase or in DNA bindingdomains of OsDmc1 [22]. The only discernible change in OsDmc1 is an insertion of seven amino acid residuesat the N-terminal region, a change also observed in Arabidopsis...
... productive binding of steroid substrates: in the first, a (small) sub population of enzyme bindsdirectly the steroid inthe reactive conformation, with the remainder of the enzyme binding the unreactiveconformation. ... unusual in havingtwo histidine residues (rather than the His-Asn pairseen in OYE, MR and some of the other memberproteins; Fig. 1B) inthe active site for ligand andsubstrate binding. For ... role inthe binding of phenolic compounds in the active site [3,26]. Likewise, in MR the counter-part residues His186 and Asn189 form key interactionswith reducing nicotinamide coenzyme and the...
... for in terms ofinteractions between binding sites in an oligomer.There is also the question of the effects of G proteins on the oligomerization process. Inthe insect cell system used in this ... conformationalchanges inthe proteins rather than changes in protein–protein interaction. A combination of several methodsseems therefore important for the demonstration of GPCRoligomerization. The D2dopamine ... (a1)2(b2)2c2,validating the use of a single derivatized antibody for the analysis of protein–protein interaction. Inthe present study,we also applied these technologies to study receptor–receptor interaction...
... Funds JESSICA in practice Spain European Investment Bank – JESSICA and Investment Funds Energy Efficiency in Buildings: Combining New Financial Instruments with the MFF The example of ... benefits from energy efficiency investment in low income housing, including eradication of energy poverty and improving quality of life. Programme promotes the establishment of housing associations, ... with any of the Op. P. of the 10 Autonomous Communities (CCAA). Belonging to one of the following areas: Buildings: Public buildings, including social housing and private buildings (residential...
... mixing, agitation and heating of the process fluid.• Claim delivers up to 50% energy savings and reduced processing time (30 mins from 60 mins) for the intensive wort boiling stage of the ... Madlener)Benchmarking Results Lager BrewingPastPapers in Brewing Journals • 1902 Journal Institute of Brewing • Concern over steam leaks, need for good lagging, finding uses for waste ... velocity steam. • Steam is introduced into a special annular "conditioning" chamber that is wrapped around the core of the PDX Reactor unit. • It is injected into the process fluid at...
... Na+binding ligand. Sodium ions may still bindto the N373L mutant through coordination to the otherligands of the binding site (center I), but the bindingbecomes much weaker as emphasized by the ... is the shift of the pH optimumfrom near neutral inthe wild-type to pH 9.2 inthe R389Amutant, which is in accord with an increase inthe pK ofY229 if the stabilizing R389 residue is lacking. ... carboxybiotin, or slow Na+move-ment through the channel to its binding site.Following the decarboxylation of carboxybiotin in the reaction cycle (Fig. 4B,C), the biotin prosthetic group leavesthe...
... that the lid is involved inthe binding of the mixed micelles of cis-PnA/NaTDC. On the other hand, theseshifts might result from the quenching of the tryptophanfluorescence in t he presence of the ... carboxylic groupand the alkyl chain between C2 and C9. The remainingatoms of the OA in molecule A, C10–C18, were included in the final protein model for the sake of overall clarity buttheir occupancies ... from the small changes in the ligand binding cavity caused by the S146A mutation.Micellar or molecular binding?It is worth noticing that the FRET technique used in the present study cannot, in...
... into the T. roseopersicinaGB2131 strain as described previously [27]. The singlerecombinants selected through their kanamycin resistancewere grown in liquid medium. The double recombinantswere ... conjugatedinto the T. roseopersicina GB1131 strain, yielding the HCMG4 strain. The double recombinants were selectedand the genotypes were confirmed as described above.Construction of HupC-expressing ... suggested for the low-potential electrons generatedduring photosynthesis [32]. The three gates for electronflow are in line with the valve hypothesis. However,depending on the sulfur source, the Hox...
... cerulean-linked elongin Band citrine-linked elongin C can be detected inthe fol-lowing donor-acceptor combinations in decreasingorder: cerulean-elongin B and citrine-elongin C >cerulean-elongin ... acceptor inthe pair of pVHL30-cerulean and citrine-elongin C is so separated that energy transfer was below the detection level.Conformational change of elongin C induced bybinding of elongin BIncreased ... four combinations: cerulean-elongin B and citrine-elongin C; cerulean-elongin B and elongin C-citrine;elongin B-cerulean and elongin C-citrine; and elon-gin B-cerulean and citrine-elongin C....