derivation and maintenance of undifferentiated human embryonic stem cells
Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... differentiation and tissue engineering, and the challenges that need to be overcome before clinical application which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells ... potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells ... Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal cell line - HPM Human umbilical...
Ngày tải lên: 11/09/2015, 09:57
The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have ... blood cells and platelets, while skin stem cells give rise to the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells...
Ngày tải lên: 16/09/2015, 15:55
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... is essential for self-renewal and maintenance of the undifferentiated state of cancer stem cells [72], indicating that this family of miRNAs has a greater role in stem cell function than previously...
Ngày tải lên: 18/06/2014, 15:20
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353...
Ngày tải lên: 11/08/2014, 12:21
Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... the study of SHH signaling in hESC offers to augment the current understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Human embryonic stem cells (hESC) ... Preparation of conditioned media from !E-MEFs 33! 3.2.3! Human embryonic stem cells and induced pluripotent stem cells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generation of stable...
Ngày tải lên: 11/09/2015, 10:14
Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells (Takahashi et al 2007; Takahashi and Yamanaka 2006) iPS cells are generated from mouse and human adult fibroblasts ... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes ... isolation and culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem...
Ngày tải lên: 04/10/2015, 16:03
Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... SAGE_Skin_melanoma_B_DB1 Undifferentiated HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 ... List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription...
Ngày tải lên: 16/10/2015, 11:58
Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and C colonies were 95.5%, 2.5% and 2.0% respectively for 48h exposure to 4oC (n=200); and...
Ngày tải lên: 31/10/2012, 16:57
báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... after ischemia Preparation of human ECs and/ or MCs derived from human ES cells Maintenance of human ES cell line (HES-3) was described previously [10] We plated small human ES colonies on OP9 feeder ... expanded cells as a mixture of ECs and MCs derived from human ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cells from ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), and these...
Ngày tải lên: 18/06/2014, 15:20
Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... Gokhale PJ, Andrews PW Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells ... limitation of adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells ... ES cells can become any and all cell types of the body and large numbers of ES cells can be relatively easily obtained in vitro culture Therefore, ES cells could be the choice of cells in stem...
Ngày tải lên: 07/08/2014, 18:21
Establishment of autologous culture systems for human embryonic stem cells
... Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem ... types of cells which are in the closely related families (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells...
Ngày tải lên: 11/09/2015, 10:00
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... and MEF-lysed matrix need to be replaced by transplant-friendly and costeffective culture systems In view of the imperfections of existing culture systems, an optimal system for the culture and ... Summary of hESC culture systems 14 1.5 Hypothesis A human ECM can be bio-assembled in vitro, decellularized and subsequently applied for the sustained growth and maintenance of pluripotency of hESCs ... hESCs [16] And such a human ECM can made by human fibroblasts, the professional ECM producer Advances in ECM deposition in our laboratory have shown that the use of MMC in the culture of human fibroblasts...
Ngày tải lên: 11/09/2015, 10:06
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... Fibronectin*† 100.00% 21 14.60% Isoform of Fibronectin*† 100.00% 15 8.51% 240832 Isoform of Tenascin*† 100.00% 5.77% 100.00% 4.54% 77311 Isoform of Transglutaminase Isoform 2C2 of Collagen alpha-2(VI) ... ig-h3 74665 122841 234698 Isoform of Extended synaptotagmin-1*† Isoform of Myoferlin*† 99.80% 4.69% Histone H1.3 Histone H1.5 100.00% 100.00% 3 17.60% 13.70% Isoform A of Prelamin-A/C 100.00% 9.04% ... IPI00418471 IPI00215743, 57920 270602 36725 53635 152453 Isoform of Myosin9*† Isoform of FilaminA* 100.00% 2.81% 100.00% 1.10% Isoform of 100.00% 3.33% 47 IPI00220967, IPI00744135, IPI00856098...
Ngày tải lên: 11/09/2015, 10:06
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs, since DCN and BGN both bind active TGFB, and could lead to a decrease ... FcNP40/DNase matrices, despite the relatively high amounts of proteins within and their complexity (refer to section 3.3), not allow the adherence of hESCs and also not maintain hESCs in a pluripotent state ... hESCs did not adhere to DxSNP40/DNase matrices, and settled on the bottom of the culture well as clusters of cells that were easily moved by mechanical disturbances to the plate...
Ngày tải lên: 11/09/2015, 10:06
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... sample, triplicates of 10,000 labeled cells were used for each marker An average of the percentage of positively-labeled cells of each triplicates was plotted, together with the standard deviation ... of hESCs on Matrigel expressed SSEA-4, with 84.2% and 88.1% for DxSDOC and DxSDOCDOC hESCs respectively Only 34.6% of hESCs cultured on Matrigel, 36.8% of hESCs cultured on DxSDOC and 37.2% of ... hESCs on DxSDOC and 75.1% of hESCs on DxSDOCDOC TRA-160 markers expression was present in 52.4% of hESCs on Matrigel, 40.9% for hESCs on DxSDOC and 49.4% of hESCs on DxSDOCDOC 36.9% of hESCs on Matrigel...
Ngày tải lên: 11/09/2015, 10:07
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
... highly expressed in undifferentiated cells, and its expression leads to the expression of other genes that maintain hES cells in an undifferentiated state DNA methylation status of the 27,578 CpG ... expression of SSEA-4 3.6.5 Pluripotency markers levels by immunofluorescence The colonies of hESCs were harvested after 13 passages for adherent immunofluorescence and stained for same panel of pluripotency ... 3.5.6, these cells can be considered as karyotypically normal due to only metaphase out of 20 having non-clonal random loss, which could have been a result of technical artifacts or random mitotic...
Ngày tải lên: 11/09/2015, 10:07
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
... Itskovitz-Eldor J Feeder layerand serum-free cultured of human embryonic stem cells Biol Reprod 70: 837-45 (2004) 26 Amit M and Itskovitz-Eldor J Feeder-free culture of human embryonic stem cells Methods Enz ... expression signatures of seven individual uman embryonic stem cell lines Stem Cells 23:1343-1356 (2005) Stewart R, Stojkovic M, Lako M Mechanisms of self-renewal in human embryonic stem cells Eur J Cancer ... Carpenter MK Feeder-free growth of undifferentiated human embryonic stem cells Nat Biotechnol 19:971-974 (2001) Evans MJ and Kaufman MH Establishment in culture of pluripotent cells from mouse embryos...
Ngày tải lên: 11/09/2015, 10:07
Baculovirus mediated genetic modification of human embryonic stem cells
... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... modification of human embryonic stem cells Although lentiviral vectors are widely used in gene transfer to human embryonic stem cells, there is still a need for vectors and methods to achieve efficient and ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...
Ngày tải lên: 11/09/2015, 14:38
Identification of oct4 and sox2 targets in mouse embryonic stem cells
... haematopoietic stem cells that give rise to blood cells (Moore and Lemischka, 2006; Petersen and Terada, 2001) (Figure 1.2) Stem cells, particularly embryonic stem cells (ESCs), offer enormous ... that of human and mice (Dailey and Basilico, 2001) Recently, the importance of Oct4 and Sox2 in the maintenance of pluripotency was further reinforced in an artificial system when Takahashi and ... binding of Oct4, Sox2, Stat3 and Smad1 in mouse embryonic stem cells as annotated in NCBI xiii LIST OF FIGURES Figure 1.1 A schematic view of mouse pre-implantation development 1.2 Mouse embryonic stem...
Ngày tải lên: 11/09/2015, 16:03
IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... allogenic) and from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells ... differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity to self renew and ... 1.5.1 Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells 16 1.5.3 Characterization of hES cells 18 1.6 Differentiation of hES cells...
Ngày tải lên: 09/10/2015, 11:24