ddt dde and ddd in vitro
Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
Ngày tải lên :
26/10/2012, 08:57
... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... (GIBCO, USA) containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All ... then evaluated by in vitro testing in human cancer cell lines We observed that 198 RGD-M-LCL had increased anti-proliferative activity and increased the induction of apoptosis in cancer cells compared...
- 12
- 635
- 0
Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
Ngày tải lên :
07/03/2014, 02:20
... first provided in a search for gastrinbinding proteins in porcine gastric mucosa [14] An interaction between Gamide and transferrin was identified by covalent crosslinking assays [14], and subsequently ... for the binding of gastrin, and of the regions in gastrin required for the interaction with transferrin, is obviously essential to a full understanding of the interaction The independent involvement ... studies showing the binding of gastrin to a transferrin with the iron-binding residues in both lobes mutated, or to the individually expressed N-lobe or C-lobe with and without the iron-binding residues...
- 9
- 543
- 0
báo cáo khoa học: "Stability and assembly in vitro of bacteriophage PP7 virus-like particles" pptx
Ngày tải lên :
11/08/2014, 00:22
... heated for two minutes at the indicated temperatures under reducing or non-reducing conditions and fractions of soluble protein and capsids remaining were determined minutes In contrast, when ... proximity within the dimer of the N-terminus of one subunit to the C-terminus of the other suggested a simple means of introducing an inter-dimer covalent bond Duplicating the coat gene and joining the ... thus creating a covalent Proteins and recombinant DNA The cloning, over-expression and purification of PP7 coat protein have been described in detail elsewhere To construct the single-chain PP7...
- 10
- 276
- 0
báo cáo khoa học: "Cationic nanoparticles for delivery of amphotericin B: preparation, characterization and activity in vitro" potx
Ngày tải lên :
11/08/2014, 00:22
... or in Milli-Q water by centrifugation (3000 rpm/10 minutes), pelleting and resuspension The final fungal cell suspension was prepared by adjusting the inoculum to × 107 cfu/mL and then diluting ... 0.01–2.00 mg/mL and 0.01–10.00 mg/mL, respectively Firstly, DODAB BF and drug were allowed to interact for 10 minutes Thereafter, CMC was added and allowed to interact for 20 minutes before adding PDDA, ... Final DODAB, AmB and CMC concentrations were 0.1 mM, 0.05 mM and 0.1 g.L1, respectively Interactions DODAB BF/AmB and CMC took place over 20 minutes and AmB/DODAB BF/CMC and PDDA, over 30 minutes...
- 13
- 774
- 0
Linear and branched chitosan oligomers as delivery systems for pDNA and siRNA in vitro and in vivo
Ngày tải lên :
23/08/2015, 17:29
... chitosans in vitro and in vivo (Paper I) Enhance the transfection efficiency of chitosan oligomer-based pDNA formulations in vitro and after lung administration in vivo by coupling a targeting moiety ... to achieve targeted gene delivery in vitro and in vivo Various ligands that target cell-surface receptors (transferrin, folate, lectin, growth factors and integrin receptors) have been coupled ... TCO polyplexes in vitro 47 Figure 15 Targeting cell-surface lectins and cellular uptake in vitro (A) Chitosan polyplexes were incubated with HEK 293, HepG2 and 16HBE14o- cells and luciferase...
- 78
- 296
- 0
Analysis, sequencing and in vitro expression of PCR products
Ngày tải lên :
25/10/2013, 22:20
... functional domain mapping of proteins where fragments that represent protein domains are expressed and functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... nonbiotinylated single strand Both the strands can be used as templates for DNA sequencing reactions The immobilized strand can be stored and reused for further reactions into solution and leaving...
- 24
- 494
- 0
Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
Ngày tải lên :
18/02/2014, 04:20
... EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro ... death (5 postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of ... endosomes containing in vivo internalized ETA The endosomal fraction was isolated 30 after the administration of ETA, and then resuspended in 0.15 M KCl containing mM MgCl2 and, when indicated, 50...
- 15
- 588
- 0
Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
Ngày tải lên :
18/02/2014, 08:20
... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids...
- 17
- 406
- 0
Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
Ngày tải lên :
20/02/2014, 01:20
... template and binding properties of mutated and deleted RNA fragments of the 3¢-end of the minus-strand HCV RNA in further detail This study should lead to interesting interpretations since it ... domain in the 3¢-end of the HCV minus-strand RNA inhibits in vitro initiation of RNA synthesis by the viral polymerase [19] Kashiwagi et al [20] studied RNA synthesis by recombinant HCV NS5B using ... unavailable In the present study we investigated the involvement of sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using new mutants of the 3¢-end of minus-strand RNA...
- 15
- 597
- 0
Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
Ngày tải lên :
07/03/2014, 06:20
... overhanging nucleotides were excised completely from the 3¢-terminus in the annealing oligomer, and consequently, no expanded band was observed during incubation Doxorubicin did not inhibit the ... as the ligand–nucleic acid complex is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B ... with the increase in reaction time, indicating that primer and template slippage occurred during DNA synthesis In the presence of DDI-1A and DDI1B, radioactivity bands (both primer and template)...
- 12
- 473
- 0
Báo cáo khoa học: Docosahexaenoic acid stabilizes soluble amyloid-b protofibrils and sustains amyloid-b-induced neurotoxicity in vitro ppt
Ngày tải lên :
07/03/2014, 10:20
... Ab aggregates, indicating a direct interaction Possibly, DHA and AA micelles work as a protective agent and prevent intermolecular interactions and consequent aggregation into insoluble fibrils ... lm) did not maintain Ab42wt-induced toxicity Ab42Arc-induced toxicity was not maintained with either a low or a high concentration of DHA (Fig 7C) DHA in itself did not result in increased toxicity, ... intermediates, i.e protofibrils, and thereby maintaining Ab-induced toxicity in PC12 cells Results Unsaturated fatty acids stabilize Ab42wt protofibrils Fatty acids were incubated with Ab42wt in...
- 11
- 465
- 0
Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx
Ngày tải lên :
07/03/2014, 11:20
... the skin or skin cells did not use the same endpoints For instance, in vitro exposure at 900 or 1800 MHz was found to affect gene expression and induce DNA damage in a fibroblastic cell line and ... dermis (DDD) extracted from skin biopsies, conserved in Hank’s buffer with penicillin ⁄ streptomycin ⁄ amphotericin and kept frozen until use A seeding cylinder was placed on a thawed DDD (in a ... possible induction of cell stress in the skin by WÆkg)1 GSM-900 exposure for 48 h No apoptosis was induced in either skin cell type, in agreement with reports of other in vitro studies concluding...
- 17
- 430
- 0
Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
Ngày tải lên :
07/03/2014, 21:20
... USA) and transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein ... tag were obtained by in vitro translation in wheatgerm extract (Invitrotec) according to the manufacturer’s procedure OsRPA70a (or OsRPA70b) and OsRPA32, or OsRPA32 and OsRPA14 were incubated with ... CM (1998) Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA Nucleic Acids Res 26, 5388–5393 17 Stauffer ME & Chazin WJ (2004) Physical interaction...
- 12
- 588
- 0
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D...
- 9
- 422
- 0
In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
Ngày tải lên :
15/03/2014, 04:20
... to the methods involved in collecting, preparing and screening plants for bioactive properties for use in manipulating key ruminal fermentation pathways and against gastrointestinal pathogens ... combination of volume, rainfall range and growth rate indices Fodder priority index – a combination of adaptability priority, fodder palatability and fodder height indices • Rainfall range index ... extrapolating in vitro findings to in vivo evaluation of plant resources The chapters in this book are written by experts interested in exploring and making better use of plant biodiversity for improving...
- 252
- 4.5K
- 0
Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... encoding regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell-based, in vitro and in silico ... providing the recombinant IKK2 (rhIKK2) and Nandini Kishore for providing the SC-514 inhibitor We thank also Paul Hayter, Sasha Sreckovic and Matthew Strawbridge with help in growing and analyzing ... In vitro analysis of NF-jB signaling pathway 46 Peet GW & Li J (1999) IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin...
- 13
- 475
- 0
Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
Ngày tải lên :
16/03/2014, 12:20
... and 30 Single-stranded (ss) and double-stranded (ds) species are indicated (B) Bar chart representing annealing percentage (%) obtained at 30 (means ± SEM, n ¼ 3) **Indicates P < 0.01 in respect ... in vitro recombinant CNBP phosphorylation in kinase buffer containing 100 lm ATP and lCi [32P]ATP[cP] at 30 °C for Kinase inhibitors analysed (Sigma Aldrich, St Louis, MO, USA) were olomoucine ... phosphorylating CNBP, we carried out in vitro phosphorylation assays using embryonic extracts previously incubated with specific kinase inhibitors Ser4 and Thr56 were predicted as putative amino acid...
- 13
- 500
- 0
Báo cáo khoa học: Reconstitution in vitro of the GDP-fucose biosynthetic pathways of Caenorhabditis elegans and Drosophila melanogaster ppt
Ngày tải lên :
16/03/2014, 13:20
... the Netherlands), which were grown overnight in 10 mL Luria–Bertani medium containing kanamycin and chloramphenicol (also containing ampicillin in the case of double transformation) In the case ... and actin using an annealing temperature of 58 °C and lL diluted cDNA For analysing transcripts encoding GDP-Fuc biosynthesizing enzymes, : diluted cDNA was used with the L1 and L2 ⁄ stages and ... Rhomberg et al neural tissue and cells in many invertebrates, including Caenorhabditis and Drosophila [9–11] Not only have the relevant fucosyltransferases and fucose-containing glycans been the object...
- 13
- 368
- 0
In Vitro Fertilization – Innovative Clinical and Laboratory Aspects Edited by Shevach Friedler potx
Ngày tải lên :
16/03/2014, 21:21
... urinary human chorionic gonadotropin in inducing final follicular maturation and ovulation in in vitro fertilization 14 In Vitro Fertilization – Innovative Clinical and Laboratory Aspects procedures: ... Hurwitz JM., & Santoro N (2004) Inhibins, activins, and follistatin in the aging female and male Seminars in Reproductive Medicine Vol 22, No 3, pp.209-217 Jasinska A., Strakova Z., Szmidt M., ... Chorionic Gonadotropin Follicle Stimulating Hormone Luteinizing Hormone Insulin growth factor 1 In Vitro Fertilization – Innovative Clinical and Laboratory Aspects granulose cells and regulate oocyte...
- 168
- 338
- 0
Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
Ngày tải lên :
16/03/2014, 23:20
... memory loss and dementia [26,27] Thus blocking Ab formation by inhibiting c-secretase is a 5551 Characterization of in vitro c-secretase activity A B D E Hoke et al C Fig Zinc inhibits in vitro c-secretase ... CL & Evin G (2004) Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex ... COS7-SPC99-3FLAG in vitro c-secretase activity While performing control reactions for inhibitor experiments, a two- to fivefold increase in the detection of products arising from in vitro c-secretase...
- 14
- 420
- 0