cytokine expression and signaling in brain tumors
Báo cáo y học: "Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis" potx
... sequence and the interplay of cytokine gene activation in AIA, therefore, the mRNA expression of monokines and of Th1-like and Th2-like cytokines was analysed in the SM, regional lymph node (LN), and ... unaffected joint (left) Assessment of cytokine mRNA expression Tissue sampling and preparation Samples of knee joint SM, inguinal LN, and spleen were obtained from rats killed at five time points: day ... performed in duplicates, which yielded comparable results Assessment of cytokine protein expression Tissue sampling and preparation In an independent AIA study, samples of knee-joint SM, inguinal...
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Tài liệu Báo cáo khoa học: Osmotic stress sensing and signaling in fishes doc
... place in the order of minutes to hours and involves many hormones, including arginine vasopressin, angiotensin II, natriuretic peptides, vasoactive intestinal peptide, urotensin II, insulin and ... (within h) induced by hyperosmotic stress, indicating a role of this mitogenactivated protein kinase kinase kinase interacting protein for osmosensory signal transduction in fish 14-3-3 proteins ... mitogen-activated protein kinase kinase kinase interacting protein (TAK binding protein ¼ TAB 2), as an IEG during hyperosmotic stress in tilapia gill epithelium [26] This gene is transiently and very rapidly...
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx
... Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin signaling Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into ... Osmosensing and signaling in mammalian cell function F Schliess et al [7] On the other hand, hyperosmotic shrinkage prevents insulin-induced hepatocyte swelling and proteolysis inhibition, indicating ... [12–14] Integrin-inhibitory peptides exhibiting an arginine-glycine-aspartic acid (RGD) motif abolish hypoosmotic osmosignaling towards Src-type kinases, mitogen-activated protein kinases (MAPKs) and...
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Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx
... receptor potential channel protein (TRPC1) (amino acids 271–349) contains a Caveolin-1-binding motif between amino acids 322 and 349 Deletion of this binding domain altered localization of TRPC1 ... washings in PBG, and a further six washings in NaCl ⁄ Pi, incubation with 2.5% (w ⁄ v) glutaraldehyde in NaCl ⁄ Pi, and further brief washes in destilled water, sections were stained by incubation ... localization in subconfluent cells was determined Figure 5A shows a Caveolin-1 staining pattern at the cell surface, confirming the known caveolar location of Caveolin-1 In addition, intracellularly distinguishable...
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Báo cáo y học: "Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus" pps
... = cytokine secretion in Dex and PHA, n = cytokine secretion in RPMI alone, y = cytokine secretion in PHA alone By using this calculation, the variations for cytokine secretion among and within ... GR expression and binding in SLE patients was lower in the SR group than that in the SS and control groups FCM was a reliable and reproducible method for measuring GR in experimental and clinical ... evaluate the GR expression or binding in individual cell types Because GR expression and binding are different in respect to blood cell type, the variation in cell type percentages in different...
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Báo cáo y học: "Parthenolide inhibits ERK and AP-1 which are dysregulated and contribute to excessive IL-8 expression and secretion in Cystic Fibrosis cells" ppsx
... Holtmann and colleagues found that introduction of an activating mutation in MKK6, increased p38 and, stabilized IL-8 mRNA and further increased IL-8 protein formation induced by NFκB-inducing kinase ... maintained in a 5% CO2 incubator at 37°C using MEM (Mediatech, Inc Herndon, VA) for AS and S cell lines and LHC-8 media (Biosource, Camarillo, CA) for IB3-1 and S9 cell lines All media contained ... we investigated the following two hypotheses: 1) To determine if the signaling pathway going through the MAPKs: p38, extracellularregulated protein kinase (ERK), and Jun-N terminal protein kinase...
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Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage" ppt
... interleukin 12 and interleukin 10 expression in vaccinia virus-infected human monocytes and U-937 cell line Cytokine 2000, 12:900-908 Jindal S, Young RA: Vaccinia virus infection induces a stress ... Figure virus-induced HSE binding activity in macrophages and a human hepatoma cell line Vaccinia3 Vaccinia virus-induced HSE binding activity in macrophages and a human hepatoma cell line (A) Macrophages ... for infected and control cells respectively HSP70 protein increases during infection and transiently accumulates in the nucleus HSP70 protein content increased during the first 14 hours of infection...
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báo cáo khoa học: " Gene expression and hypoxia in breast cancer" doc
... that can influence cell survival and growth through changes in several signaling pathways that are involved in protein synthesis, autophagy, apoptosis and metabolism [29] Intriguingly, mTOR and HIF1 ... use, including the Topoiso merase inhibitor topotecan, have been shown to inhibit HIF‑1α protein accumulation in cell lines and xenograft studies [49,50] It may be that, in the clinical setting, ... E1B 19 kDa protein-interacting protein 3; CAD, carboxy-terminal transactivation domain; CAIX, Carbonic anhydrase 9; CDKN1A, Cyclin-dependent kinase inhibitor 1A; FIH, factor inhibiting HIF; GLUT1,...
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Báo cáo y học: "Vascular Endothelial Growth Factor (VEGF) isoform expression and activity in human and murine lung injury" pps
... Several lines of in vitro evidence have pointed to a possible role for VEGF in lung repair and recovery following injury[19,29,35,36] In one LPSinduced murine model of lung injury, intrapulmonary ... depicting 3ng/ml and combination confirming a significant and cell number (B) following treatment for 48 hours with Bar Bar graphs depicting 3H-thymidine incorporation into ATII cells (A) and cell ... Immunostaining shows positive VEGF expression in alveolar epithelium (AE), alveolar macrophages (AM) and (to a lesser extent) vascular endothelium (VE) Significant increase in staining noted in ARDS,...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx
... that includes a DNA-binding domain, a ligand-binding domain and a transactivation domain The DNA-binding domain is responsible for DNA binding specificity and dimerization, and the ligand-binding ... domain involved in protein–protein interactions FYRC and FYRN domains are involved in heterodimerization between MLLN and MLLC terminal fragments High-mobility group (HMG) domains are involved in ... transformation Thus, diverse DNA-binding domains, protein–protein interaction modes and pre-existing chromatin modifications may facilitate the binding of MLLs, depending on the context and cell types, to facilitate...
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Tài liệu Báo cáo khoa học: Tachykinin-related peptide precursors in two cockroach species Molecular cloning and peptide expression in brain neurons and intestine docx
... from both brain and intestine in L maderae [14,15] The finding that N-terminally extended TKRPs are predominantly expressed only in the gut and not in the brain are in line with findings of enrichment ... efficacy in processing of two N-terminally extended TKRPs in the brain and intestine, respectively This could result in the gut-specific accumulation of such peptides, whereas in the brain cleavage ... receptors and invertebrate tachykinins: a review Zool Sci 20, 533–549 Pennefather, JN, Lecci A, Candenas ML, Patak E, Pinto FM & Maggi CA (2004) Tachykinins and tachykinin receptors: a growing family...
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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury" potx
... pathology in Gsn-L mice, only the gene expression of proinflammatory cytokines in the brains of Gsn-H mice was determined Significant reductions in brain levels of early cytokines, including IL-1b and ... http://www.jneuroinflammation.com/content/8/1/118 Page of 18 Figure Gelsolin administration protects against burn-induced proinflammatory cytokine expression in brain Elevated levels of IL-1b (A) and IL-6 ... proinflammatory cytokine transcription, without augmenting expression of anti-inflammatory cytokines Microglial cells are the primary immune effector cells in brain and play a pivotal role in neuroinflammatory...
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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc
... Plasma inflammatory cytokines and chemokines in severe acute respiratory syndrome Clin Exp Immunol 2004, 136:95-103 BGoNRPf SARS: Dynamic changes in blood cytokine levels as clinical indicators in ... ability to inhibit IFN-induced signaling [41] Curiously, this does not happen in Caco-2 cells (Fig 3A, lower right panel), suggesting cell type-specific differences in cytokine signaling Taken ... By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order to grow unhindered in the...
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Báo cáo y học: "Catabolic cytokine expression in degenerate and herniated human intervertebral discs: IL-1β and TNFα expression profile Christine Lyn Le Maitre, Judith Alison Hoyland and Anthony J Freemont" ppsx
... responding to TNFα in vivo However, no increase in TNF RI synthesis was seen during IVD degeneration or herniation In fact, a decrease in TNF RI gene and protein expression was seen in degenerate and ... During IVD degeneration and IVD herniation, an increase in the protein production for both cytokines was observed, agreeing with the two earlier studies on protein production of these cytokines ... dehydrated and mounted in XAM (BDH, Liverpool, UK)) Gene expression for IL-1 and TNFα and their cytokines in human IVDs Real time PCR was performed for genes encoding IL-1β, TNFα, IL-1 RI and TNF RI and...
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Báo cáo y học: "Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrin-linked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes" pot
... localization of integrin-linked kinase is regulated by the PINCHbinding ANK repeats J Cell Sci 1999, 112(Pt 24):4589-4599 Li Y, Yang J, Dai C, Wu C, Liu Y: Role for integrin-linked kinase in mediating tubular ... Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrinlinked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes Arthritis ... [12,13] Integrin-linked kinase (ILK), located in the cytoplasmic domain of integrins, plays a key role in transmitting mechanical signals to the intracellular compartment [13-15] Within the cells,...
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Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
... recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO cycle (18) Arginine in brain is also utilized ... AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine ... (hypobaric hypoxia) and reperfusion damage in brain The increased activities of AS and AL indicate the effective recycling of citrulline to arginine and suggest a functional role to citrulline –NO cycle...
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Báo cáo y học: "Correlations of HBV Genotypes, Mutations Affecting HBeAg Expression and HBeAg/ anti-HBe Status in HBV Carriers"
... genotype B being more predominant We had indeed observed that genotypes B and C were the main strains infecting HBV carriers in Malaysia with the former being more predominant However region-wise, ... is found mainly in Japan and Ba which is found in some other Asian countries such as China, Hong Kong, Taiwan, Thailand and Vietnam The Ba strain has recombination with genotype C in the precore ... and E respectively (Table 2) Four of the sera (7.8 %) were co-infected by genotypes B and C Table 2: Combination of genotyping results determined using methods developed by Lindh et al [14] and...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... SO (1993) ADP- and thapsigargin-evoked Ca2+ entry and protein-tyrosine phosphorylation are inhibited by the tyrosine kinase inhibitors genistein and methyl-2,5-dihydroxycinnamate in fura-2-loaded ... work investigating the interaction of P2Y12 with P2Y1 signaling [25,40] We find that P2Y12 enhances the thrombin-induced Ca2+ response in a way not only involving adenylyl cyclase ⁄ PKA inhibition, ... response, which in turn Dual P2Y12 signaling in thrombin-stimulated platelets mediates thrombin generation, this indicates that P2Y12 signaling via PI3-Kb plays a regulating role in the positive...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... pancreatic juice and intestine: inadequate for role in zinc absorption Am J Clin Nutr 35, 1–9 Evans GW & Johnson PE (1980) Characterization and quantitation of a zinc binding ligand in human milk ... present in all tested organs, with an expression ratio of 1300 : 30 : in kidney, liver and brain, respectively Interestingly, ACMSD II was not detected in brain, while no significant difference in ... Residues involved in catalysis in the bacterial enzyme and the corresponding amino acids in the human protein are pointed with arrows The mean hydrophobicity values of residues within the window...
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