... BL21(DE3) pLysS (Invitrogen) for expression Expression of recombinant proteins and immunoblotting Recombinant proteins were expressed as C-terminal tagged hexahistidine fusion proteins using the pET-expression ... phase Inhibition curves obtained with Lep d 2.0101 (s), Lep d 2.0102 (h) as indicated in the figure Serum no was used in A and B and serum no in C and D Table Concentrations (lgÆmL-1) of the inhibiting ... inhibiting allergen needed to reach 50% inhibition of IgE binding ELISA inhibition was performed as described in Materials and methods Concentrations of the inhibiting allergen needed to reach 50% inhibition...
... silent, staring closely at Mai's behind, some of them carefully poking a finger at it and then smelling their finger Mai was standing half upright, with her legs slightly apart, holding one hand between ... few dominant matriarchs and their families; the other included subordinate matriarchs and their families Being of low rank, Mozu and her offspring ended up in the second division According to ... Printed in the United States of America Seventh printing, 2003 Library of Congress Cataloging -in- Publication Data Waal, F B M de (Frans B M.), Good Natured : the origins of right and wrong in...
... considering the findings in the preliminary investigation, SNR is considered as the main QoS metric at the PHY layer to determine the appropriate sensing time for cognitive users in our cross layer ... Rashid, N M Aripin, N Fisal, and S K S Yusof, “Sensing period considerations in fading environment for multimedia delivery in cognitive Ultra Wideband system,” in Proceedings of IEEE International ... MAC scheduling is considered optimal in terms of the sufficient sensing time and the time slot allocation In the next section, we will provide more insights of the sensing mechanisms and PRR-based...
... September and December 2003 Face to face interviews were administered to mothers attending antenatal clinics in rural and urban parts of Mbarara, a district in southwestern Uganda The PMTCT program in ... Snow R: The role of HIV-related knowledge and ethnicity in determining HIV risk perception and willingness to undergo HIV testing among rural women in Burkina Faso AIDS Behav 2005, 9:243-249 20 ... Hospital antenatal clinic, an urban setting, and also from three rural health units at county level in Ibanda, Bwizibwera, and Kazo, that were each implementing PMTCT Mothers received information about...
... months and two years to prevent inbreeding Individuals not involved in breeding were kept in groups composed of approximately ten juvenile males and females in larger outdoor enclosures Animals ... among wild populations, since the infection status of a dominant male would heavily influence the status of the entire troop However, other breeding strategies exist in this speciesand influxes ... provide statistical significance, in both captive andwild animals, a higher infection prevalence was seen in females than in males This possibly indicates a predominantly sexual transmission route,...
... proteins (A) and IgE-binding components (B) present in wild- type and GM potato extracts, as measured in sensiSDS-PAGE SDS-PAGE of proteins (A) and IgE-binding components (B) present in wild- type and ... treatments in the presence or absence of heating A combination of SGF and heat treatment resulted in the disappearance of the protein bands and the IgE-binding components in extracts from wild- type and ... diseases, including asthma, allergic rhinitis, and food and drug allergy, were skin-prick tested with common inhalant allergens and with extracts from GM and wild- type potatoes In the skin-prick...
... of cross- species amplification of all 100 quail markers in chicken and guinea fowl In all, 42 loci in chicken and 20 in guinea fowl yielded analyzable PCR products that were mostly similar in ... to chickens and guinea fowl The trend in comparative mapping in poultry is taking several directions including the analysis of cDNA clones [38] and radiation hybrid mapping [20], and our results ... our initiative to develop a genetic map for Japanese quail Since crossspecies amplification indicated that several of the cross- reactive markers are informative in chickens (57.1%) and guinea...
... key role in fulfilling the increased demand for protein import into the chloroplast during photo-oxidative stress, reflecting the higher rate of protein damage and turnover, by co-ordinately up-regulating ... up-regulated following infection with Pseudomonas DC3000 (avrRpm1) [88], 292 were also detected in the flu and npq1lut2 transcriptomes with 267 induced in flu and only 69 in npq1lut2 (resulting in a far ... reports showing that thioredoxins are protective proteins that maintain the cellular redox environment [106] Others are involved in chlorophyll catabolism (At4g22920 and At5g13800), and their induction...
... novel retained introns (IntronR) RT-PCR validated 50~80% of tested events, indicating the impressive potential of the cross- species method in identifying novel AS events [9] In plants, cross- species ... genes involved in transcription, splicing, photosynthesis, disease resistance, stress, flowering and grain quality (reviewed in [12,13]) Genes involved in splicing, especially in splicing regulation, ... Filled boxes and arrows indicate exons, and lines indicate introns Green open or filled boxes indicate exons skipped or retained in certain ESTs The top black scale indicates coordinates for the...
... library screened, screening stringency, oligos used for screening and SSR mining tools, notwithstanding the innate differences in genomic organization of SSRs in different species A comparison of ... canephora; Pure line II Parents and mapping population used for testing utility in mapping analysis Parents: CXR (12) and Kagganahalla (9); Mapping population: 175 segregating progenies III Species of ... for ascertaining the germplasm diversity, genotype individualization, linkage mapping and taxonomic affinities Screening/Identification of SSR positive genomic sequences from the small insert partial...
... the mandrills in the CIRMF colony Phylogenetic tree of sequences from 21 mandrills in the colony at the CIRMF (in red) andwild mandrills (in blue) inferred as described in Figure Wild mandrills ... prevalent in several animal species, including nonhuman primates, felines, bovines and equines, in which they cause persistent infection [2-7] Simian foamy virus (SFV) infection has been reported in ... of two distinct strains of mandrill SFV, one clade including SFVs from mandrills living in the northern part of Gabon and the second consisting of SFV from animals living in the south One man...
... et al 1999) The binding sites of murine Nkx2-5 has been characterized by Chen and Schwartz and been shown to have two distinct binding sites: an NK2 domain and a homeodomain binding site(Chen et ... manipulation and microinjection of synthetic mRNA Directly injecting mRNA into the developing embryo allows a researcher to perturb a developmental gene network by overexpressing a gene, introducing a ... a DNA binding protein that acts as a dimer with itself or another family member (Kasahara et al 2001) Nkx2-5 has two DNA binding domains: a homeodomain that binds the sequence TYAAGTG and an...
... anti-Influenza virus antibodies in serum of IgE positive and negative vaccinated pediatric and adult subjects, approaching two years post vaccination The exact role of IgE in Influenza virus infection ... reported that in vaccinated individuals, 2-6 days post vaccination, serum antibody responses to trivalent (inactivated) influenza vaccine were detected, peaked 2-3 weeks post vaccination, and then ... However, an increase of H1N1 and B strains influenza virus antibodies has been found in oral fluid days post vaccination [18] Levels of influenza virus-specific ASCs in blood is low before vaccination, ...
... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain ... with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli extract The Hp and E ... activity and expression of base excision repair and mismatch repair are down-regulated both in vitro andin vivo Moreover, Hp induces genomic instability in nuclear CA repeats in mice andin mtDNA...
... for successfully translating, teaching and learning English and Vietnamese idioms and proverbs in particular and for translating, teaching and learning English as a idioms and metaphorical idioms ... data analysis - Describing and analyzing the connotation of VZAs in English and Vietnamese idioms and proverbs - Comparing and contrasting the connotation of VZAs in idioms and proverbs between ... LEARNING AND TRANSLATION OF IDIOMS AND including: “dog-chicken”, “horse-buffalo”, “dragon-snake” and etc PROVERBS VZAs appear in both English and Vietnamese idioms and 5.2.1 For Teaching and Learning...
... Vietnamese and English people Chapter Addressing Forms in Greetings in Vietnamese and English 2.1 Addressing Forms in Greetings 2.1.1 Greetings Greeting is an act of communication in which human beings ... necessary In this paper, we only deal with the characteristics of the addressing forms in greetings in Vietnamese and English and the similarities and differences in using them in cross- cultural ... cross- cultural conflicts involving the addressing forms in greetings in global communication Scope of the Study Addressing forms in communication is really an interesting issue, especially in...
... acts as following ways: * To say something is to something * In saying something, we something * By saying something we something And it is clear that in terms of speech acts, illocutionary force ... open and close ended questions, which contain three main parts: The first one is to find some general information about informants which are used to explain some unexpected findings during the ... research - The concluding and summarizing is the last one used to give final conclusions and suggestions for further study Design of the study This thesis includes three main parts and three chapters:...
... example above, we find that the using names of animalsin English and Vietnamese idioms are very abundant This makes saying and writing more rich and excited 2.1.2 The Names of Animalsin Vietnamese ... a habit of noticing idioms in everyday situations, including reading and listening Teachers lead a discussion about figurative language including metaphors, similes, and idioms in order to show ... his hometown) In conclusion, in this chapter, the author have just introduced the definitions of idiom in English andin Vietnam and presented some criteria to distinguish idioms and free- word...
... N-acetylornithine deacetylase; OCT, ornithine carbamoyltransferase; ASS, argininosuccinate synthase and ASL, argininosuccinate lyase by arginine [16,17] As a result, the concentration of citrulline and ... mitochondria [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32] In plants, information on the localization of citrulline and arginine biosynthetic ... products, citrulline and arginine Results The enzyme involved in catalysis of the fifth step of citrulline biosynthesis inwild watermelon leaves During citrulline biosynthesis, N-acetylornithine is converted...
... Hatzivassiloglou, and Dan Jurafsky 2004 Extracting Opinion Propositions and Opinion Holders using Syntactic and Lexical Cues In Computing Attitude and Affect in Text: Theory and Applications Springer-Verlag ... Linguistics (COLING), Bejing, China Richard Johansson and Alessandro Moschitti 2011 Extracting Opinion Expressions and Their Polarities – Exploration of Pipelines and Joint Models In Proceedings of the ... Vector Learning MIT Press Richard Johansson and Alessandro Moschitti 2010 Reranking Models in Fine-grained Opinion Analysis In Proceedings of the International Conference on Computational Linguistics...