cloning pcr and in vitro mutagenesis

... (amantadine and rimantadine) and neuraminidase inhibitors (NAIs; zanamivir and oseltamivir) are currently used for the chemoprophylaxis and treatment of influenza [9] The drugs should be administered ... administered within 48 hours of infection to get the optimum results Amantadine binds to and blocks the M2 ion channel proteins function and thereby inhibits viral replication within infected cells ... against the virus to control and treat infections Traditionally, the generation of new drugs involves screening hundreds of thousands of components against desired targets via in vitro screening...

Ngày tải lên: 12/08/2014, 04:21

... radioactivity indicated here, you should maintain identical ratios in first- and second-strand syntheses Our protocol results in ds cDNA of an SA of 6.67 x lo5 dpm/l.tg Single-stranded cDNA (first-strand ... 7.5, and 20% polyethylene glycol (PEG8000) 29 Photoprobe Biotin and Avidin D agarose resin (Vector Laboratories, Burlingame, CA): Resin is prepared by washing the slurry three to four times in ... 1OX kinase buffer, pL of 100 mA4rATP, pL of H20, and p.L of 10 U&L polynucleotide kinase 15 Incubate kinase reaction for 30 at 37OC 16 Heat inactivate the polynucleotide kinase by incubating the...

Ngày tải lên: 11/04/2014, 09:47

... gieo với mật độ dầy nên cấy chuyền Hạt lan Mini Dendrobium nẩy mầm sau 30 ngày phương pháp nuôi cấy mô Lan Mini Dendrobium phát triển mạnh điều kiện invitro Môi trường cấy chuyền môi trường gieo ... từ 1-2%) hạt lan chứa anbumin phôi chưa phân hóa, có kích thước nhỏ nên không chứa chất dự trữ Vì tự nhiên để hạt lan nẩy mầm cần cộng sinh với nấm Có loại nấm cộng sinh nhiều giống lan khác biết: ... paraffin - Đem để vào chỗ tối mát tuần Sau đem ánh sáng (cường độ khoảng 2000 lux = bóng đèn neon để cách khoảng 30 cm) Nếu làm tốt không nhiễm, đồng thời hạt thụ tốt vòng 30 - 40 ngày (đối với Mini...

Ngày tải lên: 04/07/2014, 23:20

... contains a highly active lysoPC acyl transferase [25,26] Acyl group incorporation into PE also occurred in all fractions, with similar rates in the ER- and plasma membranecontaining fractions In ... two-phase partition alone or in combination Physiol Plant 1992, 84:121-133 Sommarin M, Sandelius AS: Phosphatidylinositol and phosphatidylinositolphosphate kinases in plant plasma-membranes Biochim ... to the varying conditions in the plant environment The adjustments include selective lipid degradation, increased incorporation of certain lipid classes and/ or lipid molecular species and possibly...

Ngày tải lên: 12/08/2014, 05:20

... examined by an expert ophthalmologist and routine ophthalmic examinations including visual acuity determination, slit lamp examination, ophthalmoloscopy and intraocular pressure measurement were ... cataract In: Tasman W, Jaeger A, eds Duane’s Clinical Ophthalmology Philadelphia: Lippincott-Raven publishers, 2000:12-14 Kahn HA, et al The Framingham eye study I Outline and major prevalence findings ... alterations in blood, may be the intervening ones [29] Multiple studies have been done to clarify the relationship between human biochemical elements and cataract formation Interestingly in some...

Ngày tải lên: 03/11/2012, 09:49

... software, managing user accounts, and backing up and restoring files Many more topics are covered in this chapter Chapter details the steps involved in planning and building a network and planning for ... Installing and Configuring Servers Installing and Configuring Application Software Creating and Maintaining User Accounts Backing Up and Restoring Files ... 302 Installing WU-FTPD 303 Installing the binary RPM 304 Installing and building the source RPM 304 Installing and building...

Ngày tải lên: 12/12/2013, 22:15

... According to Buchbinder and Reed [21], Na+ and K+ bind to the divalent cation binding site I Thus, the inhibition shown in Fig would result from the Selectivity of pyruvate kinase for Na+ and K+ ... for K+ in water to that in 40% To gain further insight into how dimethylsulfoxide affects the competition between the monovalent cations and Mg2+ in pyruvate kinase, we determined the kinetics ... Solvation and energetic of binding of Na+ and K+ to pyruvate kinase It has been hypothesized [18] that the difference between Na+ and K+ in the activation and inhibition of pyruvate kinase could...

Ngày tải lên: 08/03/2014, 02:20

... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... (GIBCO, USA) containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All ... then evaluated by in vitro testing in human cancer cell lines We observed that 198 RGD-M-LCL had increased anti-proliferative activity and increased the induction of apoptosis in cancer cells compared...

Ngày tải lên: 26/10/2012, 08:57

... ethanol-induced expression and activation of cytokines and chemokines in monocytes and macrophages (including Kupffer cells) [41, 42], and ethanol-induced mucosal injury in the upper gastrointestinal ... protein binding double-stranded RNA binding DNA binding ▼ 5.02 protein binding NM_001149 NM_004242 ▲- increased ▼- decreased Validation of microarray results by quantitative real time RT -PCR analyses ... Interestingly, HMGN3 has been found to be associated with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma derived...

Ngày tải lên: 31/10/2012, 15:28

... functional domain mapping of proteins where fragments that represent protein domains are expressed and functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction ... strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro expression of PCR products 99 Template DNA PCR with one biotinylated primer B Biotinylated PCR product Bind ... (A) Initial PCR amplification product using two original flanking primers (primers and 2); (B) nested PCR from the primary PCR amplification product using one original flanking primer (primer 1) and...

Ngày tải lên: 25/10/2013, 22:20

... configuring servers N Installing and configuring application software N Creating and maintaining user accounts N Backing up and restoring files N Monitoring and tuning performance N Configuring a secure ... you prevent intrusion into non-Linux machines that may reside on your network Linux is designed from the beginning with security in mind, and in all of your tasks you should maintain that same ... planning and preparing for your new network installation Deciding What Kind of Network You Need Linux is, by definition and right out of the box, a network operating system It is also nearly infinitely...

Ngày tải lên: 12/12/2013, 22:15

... you up line by line instead of moving through the interpreter's history like it does in the basic interpreter Ctrl+Up moves through the history However, the history is in blocks, not lines So, ... your cut -and- pasting needs (cutting and pasting into any other editor works fine too) 10 Un-killable Processes By Johnathan Kupferer, Red Hat Certified Engineer Before Red Hat Enterprise Linux 4, ... around the definition, making changes Ctrl+Enter even allows you to insert new lines into the definition When you're done, press Enter and the class is re-defined according to your revised code...

Ngày tải lên: 21/12/2013, 04:18

... Certified Engineer® The obvious answer is that you can’t by just looking at the inode; the inode only contains a link count, not a list of the file names that are hard-linked to the inode So you ... filesystem looking for files that have the same inode number as the file of interest The not so obvious solution is that the find command can this searching for you, once you’ve determined the inode number ... Red Hat® Linux Tips and Tricks TM Introduction Are you looking for a quick and simple reference guide to help you navigate Red Hat® LinuxTM systems? Look no further Global Knowledge and Red Hat...

Ngày tải lên: 21/12/2013, 04:18

... EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro ... death (5 postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of ... endosomes containing in vivo internalized ETA The endosomal fraction was isolated 30 after the administration of ETA, and then resuspended in 0.15 M KCl containing mM MgCl2 and, when indicated, 50...

Ngày tải lên: 18/02/2014, 04:20

... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids...

Ngày tải lên: 18/02/2014, 08:20

... staining patterns (comparing and 2) and by the yellow color resulting from combination of green and red fluorescence in that iPLA2c is localized, in large part, to the mitochondrial compartment in ... iPLA2a (patatin, found in potato tubers) [28] and iPLA2b [23] – iPLA2c contains a consensus site for nucleotide binding and a lipase consensus motif in its C-terminal half [26] Although the intracellular ... 88, 77, 74 and 63 kDa polypeptides [26] However, in initial studies in baculoviral and in vitro rabbit reticulocyte lysate systems, we unexpectedly observed that constructs containing the full-length...

Ngày tải lên: 19/02/2014, 16:20

... template and binding properties of mutated and deleted RNA fragments of the 3¢-end of the minus-strand HCV RNA in further detail This study should lead to interesting interpretations since it ... domain in the 3¢-end of the HCV minus-strand RNA inhibits in vitro initiation of RNA synthesis by the viral polymerase [19] Kashiwagi et al [20] studied RNA synthesis by recombinant HCV NS5B using ... unavailable In the present study we investigated the involvement of sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using new mutants of the 3¢-end of minus-strand RNA...

Ngày tải lên: 20/02/2014, 01:20

... of tokens and average type frequencies in text sections We reserved patent data published between 1979 and 2007 for training and documents published in 2008 for tuning and testing in SMT For ... pooling data from separate tasks into a single training set Instead of a trivial enlargement of training data by pooling, we train the pooled models on the same amount of sentences as the individual ... symmetrical and asymmetrical parallel corpora In Proceedings of the 23rd International Conference on Computational Linguistics (COLING’10), Beijing, China Alexandru Ceausu, John Tinsley, Jian Zhang, and...

Ngày tải lên: 22/02/2014, 03:20