cloning of the dna empcr

... alkylation of DNA The capacity of the drug to react with the genomic DNA is inversely proportional to the concentration of GSH The higher the GSH level, the lower the amount of drug DNA adduct and ... [39] The primary objective of the present study was to investigate further the reactivity of S23906-1 towards the bionucleophiles DNA and GSH The covalent binding of the drug to GSH reduced the ... reveal that the preincubation of the drug with GSSG had no effect on the capacity of the drug to alkylate DNA, whereas the incubation with GSH considerably reduced the extent of drug DNA covalent...

Ngày tải lên: 20/02/2014, 11:20

... removal of the second half of the PolC N-terminus would help to improve the results further Therefore, we took only the fragment of the multiple sequence alignment covering the first half of the PolC ... for the E coli DnaE OB domain [12,16] The very N-terminal region of PolC and the C-terminal domain of DnaE appear to be specific for each type of polymerase The small a ⁄ b C-terminal domain of DnaE ... higher similarity to domain I of the initiator of chromosomal replication DnaA (DnaA-I) 3114 What might the function of these PolC N-terminal domains be? The involvement of related structures in protein–protein...

Ngày tải lên: 05/03/2014, 23:20

... Analysis of the phase of each of the xMGP introns located within the coding region revealed that introns and are of phase I while intron is of phase II [29] The same phases are found in the corresponding ... (Stratagene) The structure of the gene including the 5Â and 3Â anking regions was determined by double-stranded DNA sequencing, exons were identied according to the sequence of the Xenopus MGP cDNA Primer ... representation of the xMGP promoter constructs used for transient transfections of A6 cells is shown to the left The nomenclature of the promoter deletions is based on the transcription start of the xMGP...

Ngày tải lên: 08/03/2014, 10:20

... indicating the importance of the positive charges for the occurrence of nonspecific contacts with DNA [22] The BPV-1 protein shows low flexibility of the loop connecting the b2 and b3 strands (Fig 6B) The ... For HPV-16, the flexibility of the loop may alter the adaptability of the DNA- binding helices, thus modulating the discrimination of specific versus nonspecific DNA sequences For both the E2s, we ... contacts, these include contacts among the protein and the phosphodiester backbone of the DNA, solvent-mediated interactions, and the structural adaptability of the reactants An added layer of complexity...

Ngày tải lên: 16/03/2014, 10:20

... respectively) of the b subunit of glutaconate CoA-transferase protrude into a cleft on the surface of the a subunit and are involved in mediation of subunit interactions Therefore, the lack of this ... ions in MALDI-TOFMS measurements, indicating a molecular mass of 56 607 ‹ 60 Da The molecular mass of the transferase increased by 750 Da when the enzyme was incubated in the presence of 100 lM propionyl-CoA ... search, this part of the sequence showed similarity to the smaller subunits of the latter enzymes The catalytic glutamate residue of hetero-oligomeric enzymes belonging to the 3-oxoadipate CoA-transferase...

Ngày tải lên: 17/03/2014, 17:20

... (including the Fig Organization of the Mn-LOX-Gga gene, Northern and Southern blot analyses (A) The Mn-LOX-Gga gene The open box indicates the protein coding region The solid lines show the 5¢- ... explain the low number of alanine and arginine residues in the two native Mn-LOX compared to the number of these residues of their deduced precursors (Table 1) Sequence homology When the predicted ... [39] The homology of Mn-LOX and the LOX gene family included the two a helices of the latter that contain the four Fe(II) ligands These ligands are two His residues found in a helix (and in the...

Ngày tải lên: 18/03/2014, 01:20

... analyse the role of the ATPbinding domain as a modifier of transcription factors in vivo, with the prospect of developing drugs that either stabilize the ATP-bound conformation of MDM2 or inhibit the ... ng) was incubated in the presence of the indicated amounts of wild-type MDM2 protein in the absence of ATP (lanes 2–5) or in the presence of ATP (1 mM, lanes 7–10), and DNA- binding reactions ... the relative affinities of p53 and E2F1 for MDM2 are not known, thus the interaction of p53 with MDM2 might affect the level of active MDM2 that can regulate E2F1 Furthermore, the regulation of...

Ngày tải lên: 24/03/2014, 00:21

... we determined the cDNA sequences of the EcRs and the USPs (RXRs) of Chilo suppressalis [14] and Leptinotarsa decemlineata [15] Dissociation constants of the binding of PonA to these receptors ... Therefore, the elucidation of EcR and USP (RXR) structures is important for understanding the molecular mechanism of the action of 20E In this study, we report the cloning of cDNAs for EcR and RXR ... was similar to the corresponding EcR region of arthropods Subsequently, we determined the full length of the cDNA sequence by 5¢-RACE and 3¢-RACE By combining the sequences of the PCR fragments,...

Ngày tải lên: 30/03/2014, 04:20

... marker Using the marker as a guide, the kb region of the EcoR I digested MDCK DNA lane was excised from the gel followed by recovery of the DNA from the gel sample The recovered DNA was ligated ... upstream from the start of the 18S rRNA gene sequence The resulting MDCK clone then would be tested for RNA pol I promoter activity in vitro The sequence of the region upstream of the canine 18S ... nucleotide at the 5' end of the negative sense vRNA abrogates the function of this molecule Therefore, if no pol I promoter element is present in the cloned DNA fragment or if the transcription...

Ngày tải lên: 18/06/2014, 18:20

... marker Using the marker as a guide, the kb region of the EcoR I digested MDCK DNA lane was excised from the gel followed by recovery of the DNA from the gel sample The recovered DNA was ligated ... upstream from the start of the 18S rRNA gene sequence The resulting MDCK clone then would be tested for RNA pol I promoter activity in vitro The sequence of the region upstream of the canine 18S ... nucleotide at the 5' end of the negative sense vRNA abrogates the function of this molecule Therefore, if no pol I promoter element is present in the cloned DNA fragment or if the transcription...

Ngày tải lên: 20/06/2014, 01:20

... expected that the functions of canine HIPK2 were similar Molecular cloning of the cDNA of canine homeodomain-interacting protein kinase to those of human and murine HIPK2, because the deduced amino ... downregulation of p53-induced Mdm2 protein In veterinary field, the attack rate of cancer is increasing due to the longevity of pets So, the researches of cancer and p53 are highlighted and the study of ... domain there is a glycine-rich stretch of residues in the vicinity of a lysine residue, which has been shown to be involved in ATP binding In the central part of the catalytic domain there is...

Ngày tải lên: 07/08/2014, 18:21

... naked DNA into the muscle of the murine model of allergic disorder Some of the injected DNA might be postulated to stay in the nuclei of cells or be integrated in the host DNA and elicit the endogenous ... injection with 300 µg of pcDNA3.1 blank vector in 100 µl of phospate-buffered saline (PBS) (the control group) or the same amount of the mixed naked DNA encoding the major HDM allergens (the vaccination ... orchestrating the interaction of the participating cells since they are able to release an array of cytokines that can attract, prime, and activate other cell types [25] A successful outcome of immunotherapy...

Ngày tải lên: 13/08/2014, 13:22

... S Woods The Influence of the Ku80 Carboxy-Terminus on Activation of the DNA- Dependent Protein Kinase and DNA Repair is Dependent on the Structure of DNA Cofactors In mammalian cells DNA double ... in which the initial radical induced on the DNA produces a single strand break The radical is then transferred to the other strand causing the second break7 Of these two possibilities the latter ... residues These amino acids bind to about two turns of DNA along the extensive base of the molecule Further, the structure reveals that the interactions between Ku and DNA are exclusively between the...

Ngày tải lên: 24/08/2014, 09:33

... valuable overall picture of the bacterial DNA environment present in the SF of the actively inflamed joints of arthritis patients Characterization of the DNA reveals a wide spectrum of organisms so far ... indicate the number of patient set from whom bacteria were detected aAccession number of the bacterial species in the EMBL database bLength of alignment on which the 16S rDNA inserted sequence and the ... allowing the identification of the species level Sequence analysis of the PCR-positive samples revealed the presence of a wide spectrum of bacterial DNA in SF samples of all studied patients False...

Ngày tải lên: 09/08/2014, 14:22

... almost all of the OLP epithelium The count was performed by two investigators without knowledge of whether the samples were control or experimental The total number of basal and suprabasal epithelial ... in the lower-two thirds of gastrointestinal glands [15], in the more primitive testicular germ cells [28], in the transitional epithelial cells of the bladder [20], and in the basal cell line of ... of hMSH2 Therefore, the reduced expression of hMSH2 protein could make the epithelium of OLP more susceptible to DNA mutation, making it prone to oral squamous cell carcinoma development Further...

Ngày tải lên: 03/11/2012, 09:54

... hide the anti-theistic implications of Darwinism Meyer does not just make the same points in both articles; the paragraphs discussing these main ideas, comprising over two of the three pages of the ... and others – not even accept the scientific dating of the Cambrian However, even the Old Earthers, such as Behe and presumably Meyer, have offered no positive account Is their view that the “at ... As one of the philosophers who dominate the ID movement, Meyer’s work on the epistemological presuppositions of the evolution/creation debate has helped to define the core features of the movement...

Ngày tải lên: 01/11/2013, 07:20

... monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second DNA had less influence (Fig 5A) To ensure that the estimation of the protein:poly(dT) ... BSA, in the presence or absence of ssDNA or dsDNA In the ssDNA-dependent reaction, the /X174 circular ssDNA (20 lm) was used as a substrate In the dsDNA-dependent reaction, the /X174 RF I DNA (20 ... exhibit DNA- dependent ATPase activity (Fig 2E) Neither ssDNA nor dsDNA induced the ATPase activity of Rad51-Y232A, while the ATPase activity of HsRad51 was stimulated by ssDNA and dsDNA (Fig 2D) These...

Ngày tải lên: 19/02/2014, 06:20

... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs [19], which is reminiscent of the structure of ... perhaps, branch migration The proposed DNA binding role of the triple-helix domain of Ydc2 is further supported by the observation in the NCBI database that the N-terminal region of Ydc2 (residues 1–35) ... profoundly the activity of the protein However, interactions with helix could play an important functional role, such as the correct positioning of the SAP motifs in relation to the rest of the...

Ngày tải lên: 20/02/2014, 11:20

... apical membranes of the oxyntic-peptic cells [37] The distribution of the ectoATPDase on these epithelial cells is distinctly different from the other ATPDase in the E-ATPase family, the CD39s [13,17,19] ... concentration of mM at pH 7.4 On the other hand, the CaADPase activity is  80% of the MgADPase activity at a divalent ion-ADP concentration of mM (data not shown) The ATPase and ADPase activities of the ... they may have similar primary sequences in spite of their different molecular masses This proved to be the case when we obtained the cDNA of the chicken liver ecto-ATPDase by RT-PCR cloning The...

Ngày tải lên: 22/02/2014, 04:20

... whether the cellular cluster is split at the stage of totipotency of the individual cells or at the blastocyst stage In the case of nuclear transfer, on the other hand, it is doubtful whether ... violation of the protection of dignity (see Section D3 above) There is no evidence of the existence of objectives other than those of research – which, for the purposes of this Opinion, includes therapeutic ... so-called excess embryos On the basis of the fundamental right of the freedom of research enshrined in the first sentence of Article 5(3) of the Basic Law, the sanctioning of this research is now...

Ngày tải lên: 05/03/2014, 16:20