cloning expression and co expression of targeted genes

... plasmids of BoIL-2, BoGM-CSF and VP1 were correctly constructed with sequence integrity and right orientation Construction of co -expression plasmids of BoIL-2, BoGM-CSF and VP1 BoIL-2, BoGM-CSF and ... Therefore, 146 S provides complete antigens/ epitopes for the ELISA and T cell proliferation assays Cloning, expression and co -expression of targeted genes After isolation of peripheral blood mononuclear ... CFA) Mixture of VP 1and BoIL-2 (VP1+ BoIL-2) Mixture of VP1 and BoGM-CSF (VP1+ BoGM-CSF) Co- expressed product of BoGM-CSF/VP1 Co- expressed product of BoIL-2/VP1 10 Co- expressed product of BoIL-2/BoGM-CSF/VP1...

Ngày tải lên: 11/08/2014, 21:21

... The use of fenofibrate was related to significant decreases in the expression in circulating leukocytes of CX3CL1 and CCR2 genes and a significant increase in the expression of SDF-1 Page of (page ... Research and Therapy 2009, 6:26 a fasting triglyceride concentration >1.69 mmol/L) We collated pertinent HIV-related clinical and laboratory data as well as data on the course of plasma lipid profile ... association of gene expression with the use of fenofibrate Results and Discussion There were no significant differences among the three study groups for duration of HIV, age, body mass index and CD4+...

Ngày tải lên: 10/08/2014, 05:21

... transcript level of the hupTUV genes is below the detection limit or is missing in T roseopersicina Mutagenesis and homologous expression of the hupT and hupTUV genes In-frame deletion mutagenesis was ... hupT, hupU, and hupV genes (Fig 2) and flanking sequences (GenBank accession number: AY837591) The hupT and hupUV genes encode putative proteins that are most similar to HupT and HupUV of Azorhizobium ... heterodimer and the complex with HoxJ kinase, therefore the expression of the membrane bound HoxKG hydrogenase was repressed Thus uncoupling of the hydrogenase activity and the H2 sensing ability of HupUV...

Ngày tải lên: 16/03/2014, 23:20

... reflecting incomplete glycosylation as a consequence of incomplete processing, possibly in conjunction with the compromised state of the insect cell at the time of harvest In general, glycosylation of ... functionality of plasmids and for expression cloning and production of recombinant proteins on a small s c a l e 37 The most frequently used COS-1 and COS-7 cell lines are derivatives of the African green ... at the time point of transfection/infection, and the absence of mycoplasma and other contaminating agents needs to be controlled regularly The use of antibiotics and antimycotics in the cell...

Ngày tải lên: 11/04/2014, 09:41

... Figure Comparison of 2A and IRES-mediated eGFP (second gene) expression in bicistronic vectors Comparison of 2A and IRES-mediated eGFP (second gene) expression in bicistronic vectors To compare ... possibility of directing the expression of three transgenes in a lentiviral vector by using a combination of 2A and IRES sequences Tricistronic vectors were constructed with the aid of both IRES and ... HOXB4-2AMGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP To determine the efficiency of coexpression of genes, K562 and Hela cells were transduced at various MOI First Table 2: Relative expression of MGMT and eGFP...

Ngày tải lên: 19/06/2014, 08:20

... Figure Comparison of 2A and IRES-mediated eGFP (second gene) expression in bicistronic vectors Comparison of 2A and IRES-mediated eGFP (second gene) expression in bicistronic vectors To compare ... possibility of directing the expression of three transgenes in a lentiviral vector by using a combination of 2A and IRES sequences Tricistronic vectors were constructed with the aid of both IRES and ... HOXB4-2AMGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP To determine the efficiency of coexpression of genes, K562 and Hela cells were transduced at various MOI First Table 2: Relative expression of MGMT and eGFP...

Ngày tải lên: 20/06/2014, 04:20

... infection, the expression levels of IL-4, IL-13 and IL-10 in the bursa of chickens were up-regulated and peaked at dpi and then declined at dpi (Figure 3A, 3E and 3G) The change in expression of IL-10 ... coefficient (r2) for cytokines, IBDV and GAPDH are given in Table Page of Table Standard curve data from real-time PCR Genes Equation of standard curve Correlation coefficient (r2) GAPDH Y = -3.48X ... examined genes (IFN-g, IL-2 and IL-12p40) in IBDV infected birds compared with uninfected birds following infection with the H strain, the expression levels of IFN-g and IL-12p40 genes (Figure 2A and...

Ngày tải lên: 11/08/2014, 21:21

... graph of genes from GpII…………………………… 119 4-22 An expression graph of genes from GpIII………………………… 120 4-23 An expression graph of genes from GpIV………………………… 121 4-24 An expression graph of genes ... seventy-five of the probes were quality control features consisting of negative controls, performance controls, oligo synthesis controls, microarray location controls and feature morphology controls ... Attachment and entry of the virion B Uncoating and translation of the virion RNA C Proteolytic processing of the polyprotein D Synthesis of the minusstrand RNA from the virion RNA E Synthesis of nascent...

Ngày tải lên: 04/10/2015, 15:58

... regulator of viral gene expression and essential for virion production (Gruffat et al., 2002) SM protein can activate intronless genes expression and inhibit expression of intron-containing genes ... EBV latent genes, EBV lytic genes expression and their possible contribution to the development of NPC is poorly understood More and more data suggest the expression of some EBV lytic genes in ... never been a comprehensive study of EBV gene expression in NPC The aims of this study are: To construct a reliable profiling system which can detect the RNA expression of all EBV genes To comprehensively...

Ngày tải lên: 05/10/2015, 22:32

... Olofsson et al AMB Express 2011, 1:4 http://www.amb-express.com/content/1/1/4 Page of (Kilian and Uden 1988; Meinander and Hahn-Hägerdal 1997) and the affinity for xylose is orders of magnitude ... in the design of the study, constructed the strains and commented on the manuscript GL and BHH participated in the design of the study and commented on the manuscript All authors contributed to ... (2010) Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization...

Ngày tải lên: 21/06/2014, 05:20

... it seems to contain a large majority of the known genes and a bunch of predicted genes, many of which were detectable on the arrays,” says Hughes “The collection contains about 75% of the current ... bonus that we could use the microarrays to validate the expression of predicted genes and contribute to the big goal of finding all mammalian genes How long did the experiments take and what were ... annotation for thousands of genes of unknown function is a major advance.” The Hogenesch group is also creating an atlas of mammalian genes [5] “This approach had been used in yeast and worms, but...

Ngày tải lên: 06/08/2014, 18:21

... the end of July, 62% of the enriched plants ceased growth as compared with 37% of the controls The early onset of yellowing and the stopped terminal bud growth indicates earlier senescence of the ... 0.49 in the control to 0.23 in the C0 plants and total -treated dry matter increased by 43% in the C0 enriched plot 2 C0 exchange measurements Discussion and Conclusion Towards the end of the first ... chest nut trees, CO fertilization was accompa:2 nied by a decrease in leaf area and a reduction in shoot growth, which is very unusual and may have resulted from the timing of the nutrient application...

Ngày tải lên: 09/08/2014, 04:20

... structure and density statistics are shown in Table The base composition of Br and At genes is very similar The average length of Br genes (ATG to stop codon) is 73% that of At genes This is consistent ... A7 and A9, respectively, suggesting recombination of homologous counterpart regions between the 'A' and 'C' genomes in Bna Comparison of the overall composition of annotated protein coding genes ... regions [20] *Tandem duplicated genes were considered to be a single homolog †The degree of conservation was calculated by dividing the number of conserved genes by the total number of genes ‡Information...

Ngày tải lên: 09/08/2014, 20:20

... information and assisted preparation of files for GenBank submission TG co- conceptualised the project and contributed to text preparation KS co- conceptualised the project and developed and contributed ... SNP and RFLP loci [21,50] QTL based analysis and genetic mapping in wheat identified co- location of DR and R genes at qualitative disease resistance loci [70,71] Co- location of R genes with DR genes ... primary drafts of the manuscript, and contributed to finalisation of the text and journal-specific formatting NC co- conceptualised the project and contributed to data analysis and text preparation...

Ngày tải lên: 12/08/2014, 03:20

... β-tubulin, PP2A, and SAND, with Ct values of 26 or higher The calculated coefficient of variance of the Ct values gives an indication of the expression stability of a particular gene β-tubulin and cyclophilin ... two of their most stable reference genes represented both high and low expression levels compared to the genes being analysed, demonstrating that the level of expression of the reference genes ... software (Applied Biosystems, Foster City, CA, USA) with melting temperatures (Tm) of 58–60°C, primer lengths of 20–24 bp, and amplicon lengths of 61–150 bp The majority of the primer pairs targeted...

Ngày tải lên: 12/08/2014, 05:20

... Interactions of recombinant CRP-1 and -2 Comparison of expression efficiencies of rCRP-1 and -2 Interactions of CRPs are enhanced in the presence of calcium, as well as during infection CRP-1 and -2 ... The complexity of the pathogenesis of septicemia remains the greatest obstacle to its prevention and treatment 14 FIG 1.2: The consequence of sepsis include over production of cytokines and uncontrolled ... hemocytes and hemolymph of the horseshoe crabs 2.1 Primers used in the cloning of CrCRP-1 and 31 2.2 Proteins used for calibration of MALDI TOF MS/MS 51 3.1 Assessing the expression and purification...

Ngày tải lên: 03/10/2015, 20:57

... CRM analysis and designed the scoring strategy MB conceived of the study, and participated in its design and coordination All authors contributed to the writing of the paper, and read and approved ... Figure Expression analysis of 107 presynaptic genes across mouse tissues and developmental stages Expression analysis of 107 presynaptic genes across mouse tissues and developmental stages Expression ... high copy numbers of the EGFP reporter (data not shown) Based on the consistent neuronal expression of EGFP reporter, we concluded that the 5.5 kb region covering Rab3A coding sequence and five...

Ngày tải lên: 14/08/2014, 21:20

... the time and sites of PrP1 and PrP2 action(s) during nervous system morphogenesis and also suggest that PrP2 has a pleiotropic role in the course of embryogenesis A high expression of zebrafish ... prions uptake of mammalian origin by the fish intestine Comparison of zebrafish prp1, prp2, and prp3 developmental expression patterns The developmental expression patterns of prp1 and prp2 coding for ... stretch of 501 Expression of prion genes in the developing zebrafish E Cotto et al Fig The prion protein (PrP) family in vertebrates Schematic diagram of tetrapod PrPs, long (PrP1 and PrP2) and short...

Ngày tải lên: 19/02/2014, 16:20

... length and encodes a 180 amino acid protein similar to that of torafugu and mammalian counterparts Compared with other family members containing the IL-10 gene, human IL-19 and IL-24 encode longer ... of the specific bands All PCR reactions were performed according to the following protocol: lL of dNTPs (10 lM of each dNTP), 0.5 lL of ExTaq polymerase (5 UÆmL)1; Takara Bio Inc, Japan), lL of ... rainbow trout, Oncorhynchus mykiss, and analysis of its expression under conditions known to induce apoptosis Dev Comp Immunol 25, 303–312 25 Mosmann, T (1994) Properties and function of interleukin-10...

Ngày tải lên: 20/02/2014, 02:21

... other hand, it was inhibited by high concentrations of azide, an inhibitor of CD39 and most ecto-ATPDases [29–31], and high concentrations of fluoride, vanadate, and pyrophosphate, inhibitors of the ... followed by 35 cycles of 94 °C for 45 s, 55 °C for and 72 °C for and ending with 10 at 72 °C A PCR product of  1.5 kb that corresponded to the length of the coding region of the chicken ecto-ATPDase ... 200 mL of chromatography buffer containing 1% a-methylmannoside Fractions containing ATPase activity were pooled and applied to a second DEAE Biogel A column (1.5 · 10 cm) as a means of concentrating...

Ngày tải lên: 22/02/2014, 04:20