classes of cytosolic sulfotransferase involved in the bioactivation of xenobiotics
Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt
Ngày tải lên :
23/03/2014, 04:20
... than the other compounds (Fig 6) In this way, amino acid residues of different segments of NADP-ME2 swapped in the construction of the chimeras are involved in the fumarate activation of the pyruvate ... of CoA or acetyl-CoA The results are presented as the percentage of activity in the presence of the effectors in relation to the activity measured in the absence of the metabolites for each of ... activity in the presence of the effectors in relation to the activity measured in the absence of the metabolites, for each of the respective enzyme constructs The assays were performed at least in...
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Risks Involved in the Use of Herbal Products
Ngày tải lên :
25/10/2013, 05:20
... aspirin or other NSAIDs may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in blood sugar levels (hypoglycemia) Ginseng may intensify ... making it less effective Ginger taken with anticoagulants may increase the risk of bleeding Ginkgo taken with anticoagulants or with aspirin or other NSAIDs may increase the risk of bleeding Ginkgo ... from their ability to inhibit phase I and induce phase II carcinogen metabolizing enzymes that initiate carcinogenesis They also inhibit the promotion stage of carcinogenesis by inhibiting oxygen...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
Ngày tải lên :
20/02/2014, 11:20
... responsible for binding and proper orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring of ThDP ... structure Binding of the cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules of the cofactors ThDP and Mg2+ The ThDP binding sites are located in narrow clefts at the interfaces ... to these interfaces is located in the PYR and middle domains The interface contains 10 hydrogen bonds in IPDC The dimer–dimer interface in IPDC is smaller than the corresponding interface in...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
Ngày tải lên :
21/02/2014, 01:21
... implicated in the biosynthesis of the aminonucleoside moiety of A201A Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this ... be implicated in the biosynthesis of the aminonucleoside moiety of puromycin [6–8] Interestingly, their organization is identical to that of the pur cluster, with the exception of pur6, which ... [33,34] In contrast, its similarity to type III PKSs, which lack this domain, is scant This domain promotes the binding of the acyl-CoA initiation unit to the ketosynthetase domain of the PKSs...
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
Ngày tải lên :
22/02/2014, 07:20
... listed in Table that most, if not all, of the amylose is synthesized in vivo when the rate of amylopectin synthesis becomes minimal At these moments the rates of amylopectin synthesis are only 5% of ... rate of amylopectin synthesis The increase in crystallinity witnessed during these first 12 h is in line with the high rates observed for amylopectin synthesis However between 12 and 58 h the rate ... amylose continues to accumulate at the final stage of starch synthesis in the absence of concomitant amylopectin synthesis (reviewed in [41]) In vitro synthesis of amylose Because in Chlamydomonas...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
Ngày tải lên :
07/03/2014, 16:20
... addition of scintillation fluid Nonspecific binding was determined in the presence of lm unlabeled agonist and subtracted from the total binding to calculate the specific binding Internalization of [3H]BK ... stimulated binding of GTPcS to Gq ⁄ 11 [30] All of these data point to an involvement of putative helix in the interaction with cognate G proteins As both bradykinin receptors coupled to the same ... expressed in percentage of total binding, decreasing the binding affinity of the FEBS Journal 272 (2005) 129–140 ª 2004 FEBS A Faussner et al Role of helix and C-termini in bradykinin receptors Fig Schematic...
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Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf
Ngày tải lên :
08/03/2014, 08:20
... The fa value for KI was only 12% implying the low solvent accessibility of tryptophan residues of d2-crystallin in the native form ANS binding Fig Equilibrium unfolding of duck d-crystallin in ... the formation of dimers during the dissociation step (Fig 1) These results are in agreement with the double dimer structure of the tetrameric d2-crystallin The crystal structure of the protein ... is the dynamic quenching constant and fa was the fractional maximum accessible protein fluorescence ANS binding assay The exposed hydrophobic surfaces of the protein were assayed by incubating the...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
Ngày tải lên :
08/03/2014, 10:20
... GalNAc-T1, might be involved in the catalytic function of the enzyme To investigate whether the cysteine residues modified by PCMPS are involved in the binding of UDP-GalNAc, we treated recombinant GalNAc-T1 ... University) [22,23], containing a cDNA encoding the insulin signal sequence and the Protein A-IgG binding domain, was inserted into NheI-HindIII digested pcDNA3.1, producing the vector, pInsProA pDN42 was ... PCMPS in the presence of increasing concentrations of UDP-GalNAc (d) and UDP (s) Following incubation, the enzyme activity was determined as described in Fig Mutagenesis of the cysteine residues in...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
Ngày tải lên :
16/03/2014, 22:20
... treatment The high degree of protection by ADP suggests that the mitochondrial injury is caused by opening of the mPTP There is evidence that ADP inhibits opening of the mPTP by occupying binding sites ... the induction of oxidative stress during ischemia ⁄ reperfusion in brain There is a growing body of information concerning the mechanism of ROS generation by the respiratory chain in mitochondria ... during H ⁄ R performed in the presence or absence of 3.5 lm Ca2+ The mitochondrial incubations were carried out in the cuvette of the luminescence spectrophotometer at 30 °C in the presence of...
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Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
Ngày tải lên :
16/03/2014, 23:20
... success in expressing a recombinant XDH in this organism Expression of XDH requires the incorporation of the MPT center, placement of the terminal sulfur ligand on the Mo, and the incorporation of ... stoichiometric and independent of the presence of xdhC, and their data suggest that the level of MPT sulfuration is lower in the absence of xdhC than in its presence Assuming that the level of incorporated ... effect of the level of culture aeration on the expression of recombinant protein Raising the cellular redox potential by increasing aeration levels is expected to reduce the level of cysteine and increase...
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Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
Ngày tải lên :
17/03/2014, 03:20
... that of the active area in insulin (Fig 7F) If the mechanism of the interaction between the 4-kDa peptide and 43-kDa protein has the minimum components of insulin–insulin receptor interaction, the ... similar to the insulin– insulin receptor interaction system Interaction of insulin with the 43-kDa protein Acknowledgements Similarly to the 4-kDa peptide, insulin is able to interact with the 43-kDa ... Nakagawa, S.H & Tager, H.S (1987) Role of the COOHterminal B-chain domain in insulin–receptor interactions: identification of perturbations involving the insulin mainchain J Biol Chem 262, 12054–12058...
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Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf
Ngày tải lên :
17/03/2014, 10:20
... that of the background The binding site was found to be located at the C-terminus of the protein although binding of the C-terminus half (CH) is only half of the full-length protein (Fig 3) Interestingly, ... only one, the atpF intron, while CRS2 is involved in the splicing of nine of the 10 chloroplast group IIB introns [6,15,37] The expression of CRS2 in E coli together with the corresponding RNA ... (35) is indicated promote the splicing A possible model of how HCF152 is involved in the stabilization of the splicing products of the petB intron could be that the protein binds to the UTR region...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
Ngày tải lên :
22/03/2014, 21:20
... the synthesis of amylopectin, it has been suggested that SSI is mainly involved in the synthesis of small chains of this fraction Furthermore, SSIV has been found recently to be involved in the ... interaction between the N- and C-terminal domains of SSIII in vitro Mapping of the CD-binding region in the N-terminal SBDs In order to identify the SBD region required for the SBD–CD interaction, we ... in the D2 domain and D(495–535) in the D3 domain This result is in agreement with our previous studies using truncated SSIII isoforms, showing the importance of the D23 domain in starch binding...
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Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf
Ngày tải lên :
23/03/2014, 03:20
... analyses of the relative levels of the indicated proteins after western blotting (as in D) were carried out Similar experiments were performed five times and the intensity of the individual bands in ... subcellular compartments other than lipid droplets The expression of CIDEC is elevated during the differentiation of adipocytes To gain further insight into the roles of CIDEC in adipocyte differentiation, ... obtained from the patient The COS-7 and 293T cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) Isolation and induction of pre-adipocytes Pieces of adipose...
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Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc
Ngày tải lên :
23/03/2014, 04:20
... whether Synoviolin is involved in the degradation of PS cofactors using synoviolin-null cells, as PS cofactors undergo the ubiquitin ⁄ proteasome pathway We report that Synoviolin is involved in ... polyglutamine-expanded huntingtin [18], the tumor suppressor Synoviolin is involved in the degradation of nicastrin gene p53 [19] and Parkin-associated endothelin receptor-like receptor [20] In this ... trafficking of NCT within the Golgi compartment, because the maturation of the sugar modification of the protein occurs within the Golgi compartment Recently, there has been an expansion of the recognized...
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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf
Ngày tải lên :
29/03/2014, 21:20
... are involved in the transmission of mechanical signals to signaling pathways In the M line, mechanical stimulation activates the interaction of a protein complex with the kinase domain of titin, ... pathway and an increase in muscle fiber apoptosis [8] The participation of NF-jB signaling in the pathogenesis of LGMD2A is therefore an interesting possibility The findings obtained in the present ... the binding to titin by improving the accessibility of the titin-binding domain Interestingly, the importance of CARP interaction for its function was recently demonstrated in a FEBS Journal...
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Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt
Ngày tải lên :
30/03/2014, 03:20
... structure of the NOX1 gene and its splicing pathway Open boxes indicate exons Numbers in the box denote the size of each exon (bp) The size of the intron is indicated under the broken lines (bp) ... be involved in an angiotensin II-induced vascular hypertrophy [15], and in inducing the expression of monocyte chemoattractant protein-1 [16], which plays a key role in the development of atherosclerosis ... in the promoter region of the NOX1 gene, ATF-1 was assumed to indirectly regulate the expression of the NOX1 mRNA We therefore elucidated the role of ATF-1 in the MEF2B-dependent activation of...
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Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
Ngày tải lên :
30/03/2014, 04:20
... residues were individually mutated (C) The spacial proximity of active site residues in the L-domain and connecting region II which are potentially involved in the binding of D-glucose in the super-open ... E290) and the S-domain (residues T168 and K169) (Fig 1B) To identify the contact residues involved in the initial binding of Glc to the super-open state (Fig 1C) of this two domain hinge-bending enzyme, ... demonstrating that the binding of Glc at the active site indeed induces a large-scale domain movement that closes the active site cleft and creates the stereochemical environment for binding of the...
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Báo cáo khoa học: Towards understanding the functional role of the glycosyltransferases involved in the biosynthesis of Moraxella catarrhalis lipooligosaccharide ppt
Ngày tải lên :
30/03/2014, 08:20
... Lgt4 Results In order to investigate the function of the glycosyltransferase enzymes involved in the biosynthesis of LOS of M catarrhalis, and to gain some insights into the mechanism of serotype ... irradiating each of the anomeric signals in turn to obtain the H chemicals shifts of the corresponding remaining ring protons Again, assignment of the Kdo residue was possible from examination of ... obtain the 13C shift of the C1 and C2 resonances of Kdo Examination of the 400 ms NOESY spectrum confirmed each of the glycosidic linkages for the 2951lgt1 ⁄ 4D oligosaccharide and the terminal...
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Báo cáo khoa học: Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus potx
Ngày tải lên :
30/03/2014, 09:20
... for the enzyme [21] These facts strongly suggest the involvement of the enzyme, GGR, in the biosynthesis of membrane lipids in the hyperthermophilic archaeon A fulgidus, as predicted in the previous ... Garmany) The flow rate of the scintillation cocktail was 0.5–2 mLÆmin)1 Extraction of archaeol from H salinalium H salinarum was cultured in L of culture medium, containing g of casamino acids, g of ... expression of AF0648 resulted in a change in the quinone profile of the host E coli, which led us to conclude that the menaquinone-specific PR is encoded in AF0648 However, the functions of the other...
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