ciliophora hypotrichida isolated from bathyal anoxic sediments
GENOTYPING OF CLOSTRIDIUM PERFRINGENS ISOLATED FROM CATTLE AND PIGS WITH DIARRHEA IN HANOI AND SURROUNDING AREAS, VIETNAM
... production properties of 3.1 Prevalence of C perfringens isolates from cattle and pigs with and without Prevalence of C perfringens isolated from fecal samples were shown in Table diarrhea were 85.33%, ... 75 C perfringens isolates from diarrheic cattle, 57.34%, 41.33%, and 1.33% belonged to type A, type D, and type C, respectively; whereas all C perfringens isolated from fecal samples of healthy ... positive C perfringens isolates were originated from diarrheic cattle The percentage of cpb2 and both cpe / cpb2 genes positive C perfringens isolated from diarrheic pigs were 45.39% and 17.11%,...
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Tài liệu Báo cáo khoa học: Reconstitution of coupled fumarate respiration in liposomes by incorporating the electron transport enzymes isolated from Wolinella succinogenes docx
... Phosphatidylcholine was prepared from egg yolk according to Singleton et al leaving out the chromatographic steps [20] Di-palmitoyl phosphatidate was purchased from Fluka MK was extracted from the membrane ... suggested by the crystal structures of related enzymes The periplasmic Nickel hydrogenases isolated from sulfate reducing bacteria consist of two subunits which are similar to HydA and HydB of ... As seen from its crystal structure, the subunits of the E coli enzyme are arranged as depicted in Fig [14] A cavity in the catalytic subunit of E coli formate dehydrogenase-N extends from the...
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Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx
... obtained from the American Type Culture Collection Trypticase Soy broth was from Fisher Chemical Co a-Glucosidase and a-galactosidase were purchased from Sigma Chemical Co Whatman 3MM paper was from ... trehalose, has been isolated from the cell wall lipids of Mycobacterium kansasii [1] That study strongly suggests that some of the oligosaccharides described here, and isolated from the cytoplasm ... 2–8, the isolated oligosaccharides designated CO1 through CO7 Ó FEBS 2002 Cytoplasmic trehalose oligosaccharides (Eur J Biochem 269) 3145 Table Amount of oligosaccharides isolated from 100g...
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Báo cáo khoa học: A new phospholipase A2 isolated from the sea anemone Urticina crassicornis – its primary structure and phylogenetic classification pptx
... kDa, respectively This PLA2 clearly differs from the one from A pallida 2642 Here we report on the isolation, cloning and characterization of a novel PLA2 from the northern Pacific sea anemone Urticina ... to original US government works New PLA2 from Urticina crassicornis A Razpotnik et al A B C D Fig Isolation of native UcPLA2 Native UcPLA2 was isolated from the exudate in a three-step chromatography ... A novel PLA2 was purified from the milking exudates of several U crassicornis specimens On the basis of the N-terminal sequence of the isolated protein, its cDNA was isolated, sequenced, and recombinantly...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... 5592 shift changes in residues from the grafted sequences However, residues from the host protein CD2.D1, such as T97 ad G107, remained unchanged The isolated EF-hand from STIM1 forms dimer in solution ... and presence of Ca2+ Results and Discussion The isolated EF-hand motif from STIM1 retains its helical structure The helix–loop–helix EF-hand motif from STIM1 was grafted into CD2 with each side ... synthesis or cleavage Shaw et al [9] first reported that an isolated EF-hand III from skeletal troponin C dimerizes in the presence of Ca2+ EFhands from parvalbumin and calbindin D9K have also been shown...
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Báo cáo khoa học: Detailed structure of lipid A isolated from lipopolysaccharide from the marine proteobacterium Marinomonas vaga ATCC 27119T pot
... of lipid A from M vaga Extraction of defatted M vaga ATCC 27119T cells with phenol/water [22] yielded LPS in the water phase According to the TLC data, the crude lipid A isolated from LPS by ... elucidation of lipid A from the marine proteobacterium M vaga To the best of our knowledge, this is the second complete structure of lipid A from a marine bacterium (lipid A from wild-type Ó FEBS ... acid has an amide-type of linkage [signals at m/z 332 and 314.4, which result from the loss of the C10 : (3OH) residue from the fragment ions at m/z 520.59 and 502.56, are in good agreement with...
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Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf
... in several mammalian cell lines: TE671 (from human cerebellar medulloblastoma), COS7 (from monkey kidney fibroblasts), GH3 and cerebellum granular cells from rat, using the technique described ... insight concerning the evolutionary links with other peptides isolated from the Buthidae scorpions The only plausible indication emerging from this analysis is that the C-terminal arrangement of this ... were edited and aligned using CLUSTAL-X [21] BLAST Gene cloning of phaiodotoxin Total RNA was isolated from venomous glands situated at the last postabdominal segment (telson) of one Anuroctonus...
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Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
... information was obtained from LC ⁄ MS ⁄ MS analyses of peptides generated from digestions using trypsin and V8 acid protease Four interpretable mass spectra were obtained from collision-induced ... strain used in this report A Delta class GST from the German cockroach Detection of IgE against GSTs from the German cockroach A pooled serum sample obtained from a panel of 16 patients allergic to ... Discussion A novel GST has been identified and purified from the German cockroach in this study Amino acid sequences obtained from the purified GST as well as from cDNA 1798 clones suggested that the enzyme...
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Báo cáo khoa học: Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells pot
... carcinogenesis are indole-3carbinol from cruciferous vegetables such as brussel sprouts and broccoli [12], curcumin from the root of Curcuma [13], and epigallocatechin gallate from tea [14] Some of these ... [30,31] Resveratrol is present in grapes, red wine and peanuts, brassinin is from Chinese cabbage, and deguelin is from an African plant, Mundule sericea The success of these natural products ... of information about its expected antiproliferative activity, nontoxic nature, and from information received from the population that uses the plant for medicinal purposes Then, selected plant...
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Antibiotic resistant enterococci and staphylococci isolated from flies collected near confined poultry feeding operations potx
... staphylococci and enterococci isolated from flies were obtained from the homogenized samples (i.e., internalized bacteria), and approximately one-third were obtained from exterior washes The results ... isolates from poultry litter and two isolates from fly samples The nearly homologous staphylococcal gene, msr(A/B), was observed in four isolates from poultry litter and one isolate from fly ... Tn1545/916, were recovered from four enterococcal isolates from poultry litter and six from fly isolates, all of which also contained the erm(B) gene (Table 4) Two fly isolates from traps and placed...
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Báo cáo Y học: Recombinant pronapin precursor produced in Pichia pastoris displays structural and immunologic equivalent properties to its mature product isolated from rapeseed pptx
... addition, rproBnIb has been found to bind IgE from the sera from allergic individuals Isolation of total RNA and cDNA synthesis Total RNA was isolated from rape seeds (Herborem) as described previously ... Sera and antibodies Sera from hypersensitive untreated individuals who exhibited positive skin-prick test and RAST (classes 3–6) to mustard seed extract, and a serum from a patient allergic to ... rproBnIb (diluted : 1000 and : 80 000, respectively), a pool of sera from patients allergic to mustard (diluted : 10) and a serum from a patient allergic to rapeseed flour (diluted : 10) The signal...
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Báo cáo Y học: Chemical structures and immunolocalization of glycosphingolipids isolated from Diphyllobothrium hottai adult worms and plerocercoids ppt
... Ten GSLs were isolated from adults (less polar ones shown in Fig 2A; more polar ones in Fig 2B) and designated as A-1 through A-10 (ÔAÕ stands for adult) Five GSLs were isolated from plerocercoids ... GalSEGLx Lane 4, total GSLs from adult worms Lane 5, total GSLs from plerocercoids Monohexosylceramides GSLs corresponding to CMH were purified as three fractions from adult worms (Fig 2A) GLC ... globotriaosylceramide, and globotetraosylceramide was purchased from Matreya A standard mixture of partially methylated alditol acetates was from BioCarb Anti-paramyosin mAb PM was donated by T Nakamura...
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Báo cáo " An active PAH-degrading microbial consortium developed from dioxin-contaminated sediments via enrichment technique " pdf
... homogenous stage of medium containing carbazol was shortened obviously from the first transferring step to the last one (from weeks to days) Moreover, the growth of microbes in the enrichment ... Degradation of polycyclic aromatic hydrocarbons (PAHs) by a bacterial consortium enriched from mangrove sediments Environ Intern 31 (2005) 149 [16] Dang T C H., Mai A T., Nguyen Q V., Trinh K S., ... characteization of new bacteria degrading high-molecular weight polycyclic aromatic hydrocarbons isolated from a 2-year enrichment in a two liquid-phase culture system J Appl Microbiol 94 (2003) 301...
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Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus pptx
... antibacterial peptide, moronecidin, isolated from the skin and gill of hybrid striped bass, exhibits a broad spectrum of antibacterial activity [10] A lysozyme-like peptide from rainbow trout (Oncorhynchus ... 65-kDa glycoprotein isolated from the rainbow trout (O mykiss, formerly Salmo gairdneri), has also been found to have strong antibacterial properties [15] Glycosylated proteins from the hydrophobic ... antibacterial fractions from Phenyl Sepharose chromatography; II, pooled antibacterial fractions from gel filtration chromatography; III, pooled antibacterial fractions from chromatofocusing The...
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Báo cáo khoa học: A new highly toxic protein isolated from the death cap Amanita phalloides is an L-amino acid oxidase pdf
... studying toxic proteins isolated from the fruit bodies of the death cap A phalloides, we detected a novel cytotoxic protein that differed from all previously described toxic proteins from that mushroom ... peptides PCR reactions with different combinations of primers were performed with cDNA from mRNA isolated from the whole fruit body The primer combinations TTC CCA GAG ATC GAG TCA ATG CGT (3¢- ... protein, toxophallin, was isolated from the fruit body of the death cap A phalloides [16] Its physico-chemical, chemical and biological properties differ distinctly from those of the other known...
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Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii pdf
... residues determined by N-terminal degradation from full-length pyridylethylated proteins The dashed line shows sequence results obtained from an HPLC -isolated peptide after V8 protease digestion ... pulses of 30 s and watts) Inclusion bodies were isolated by centrifugation (12 000 g, 10 min, C), and several ể FEBS 2004 Prolyl endopeptidase inhibitor from P sauvagii (Eur J Biochem 271) 2119 contaminants ... the cooperativity of the effect Hemagglutination Hamster, mouse and human erythrocytes were isolated from blood anticoagulated with EDTA, and, after removal of plasma and buffy coat by mild centrifugation,...
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Báo cáo sinh học: " Phenotype and envelope gene diversity of nef-deleted HIV-1 isolated from long-term survivors infected from a single source" pptx
... represent sequences cloned from subjects C98, C18 and D36, respectively E, clones from early viruses; L, clones from late viruses C18E and C98E clones cosegregated separately from C18L and C98L clones, ... remained a long-term nonprogressor HIV-1 isolated from PBMC on consecutive occasions was used in this study (Table 1) The time between isolations ranged from to years For the purpose of this report, ... highlight the heterogeneity in replication capacity by nef-deleted HIV-1 strains isolated from multiple subjects infected from a single source Coreceptor usage The results of the preceding experiments...
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Báo cáo sinh học: " Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus)" ppt
... mostly on the source of the virus isolate; id est Parapoxviruses isolated from sheep will be classified as orf virus, virus isolated from cattle as pseudocowpox [11,12] However, several molecular ... congruent with our results The close clustering of the translated sequences obtained from virus isolated from reindeer (two Norwegian; 1999, 2000, and one Finnish; 1994) with the amino acid sequences ... isolates), Johan Krogsrud (isolates from cattle), Terje Josefsen, Karen Sørensen and Torill Mørk (isolates from sheep and goats), Arnoldus S Blix (isolates from musk oxen), Colin McInnes (orf...
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báo cáo hóa học:" Tissue specific characteristics of cells isolated from human and rat tendons and ligaments" pptx
... to compare human tendon/ligaments from different sources, cells from pairs of ACL and PTs isolated from the same subjects and during the same procedure were isolated They were then compared in ... to dexamethasone between tendon cells isolated from different tendons but from the same subject To further characterise any intrinsic differences in cells from different tendons we looked at the ... stated otherwise, all chemicals were purchased from Sigma-Aldrich (Poole, Dorset U.K.), tissue culture media from Lonza (Wokingham, U.K.) and plasticware from Nunc (Nottingham, U.K.), or Greiner Bio...
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