chuyên đề studying gene expression and function

... [30,31] However, our in vitro mutagenesis and expression study did not link these mutations to an altered b2GPI gene expression [32] Although, in vitro mutagenesis and expression study not rule out ... mutation and is associated with significantly lower b2GPI plasma and mRNA levels and the low occurrence of APA Furthermore, this mutation reduces the expression of the luciferase (Luc) reporter gene ... genotype (lanes 1, and 4) Effect of the )1CfiA mutation on reporter gene expression To further confirm that the )1CfiA mutation is associated with low expression of the b2GPI gene, we performed...

Ngày tải lên: 31/03/2014, 07:20

... plants (shrubs, trees) [20] As genetic selection and breeding activities in trees are being expanded to include genetic mapping and molecular markers, candidate genes like HD-Zip III are considered ... doi:10.1186/1471-2229-10-273 Cite this article as: Côté et al.: Gene family structure, expression and functional analysis of HD-Zip III genes in angiosperm and gymnosperm forest trees BMC Plant Biology 2010 ... to the two major phylogenetic clades RVB and C8, each of which is divided into two subclades [27] Floyd et al (2006) [21] and Prigge and Clark (2006) [22] conducted phylogenetic investigations...

Ngày tải lên: 11/08/2014, 11:21

... observed between function and tissue specificity is accounted for by the age of the gene The relationship between phyletic age and tissue specificity is not explained by a gene' s function (test ... expressed Figure Expression distribution of mouse proteins functional groups Expression distribution of mouse protein functional groups (a) The metabolic functional group includes enzymes and transporter ... hypothesis that function and specificity are independent of given age Conceptually, the genes are divided up according to age and a separate contingency table for specificity and function is formed...

Ngày tải lên: 14/08/2014, 14:21

... compilation ª 2006 FEBS 3673 Expression and function of Noxo1c R Takeya et al Expression of Noxo1b and Noxo1c in various human tissues The expression patterns of the Noxo1b and Noxo1c messengers were ... FEBS R Takeya et al Expression and function of Noxo1c A B C D F E G FEBS Journal 273 (2006) 3663–3677 ª 2006 The Authors Journal compilation ª 2006 FEBS 3667 Expression and function of Noxo1c ... Noxo1b and Noxo1c (data not shown) FEBS Journal 273 (2006) 3663–3677 ª 2006 The Authors Journal compilation ª 2006 FEBS 3665 Expression and function of Noxo1c R Takeya et al Expression of Noxo1b and...

Ngày tải lên: 19/02/2014, 06:20

... discussions and Edith Trejo and Carlos Zadikian for technical assistance We are indebted to Drs J J Cazzulo and C Labriola for their generous gifts of a cruzipaincontaining plasmid and primers A and ... [10] In the present Foreign ADC gene expression in T cruzi work we used ADC-transgenic protozoa to follow the time-course modulation of foreign gene expression and to investigate whether this ... the parasite genome organization Results and Discussion Expression of the oat ADC gene in T cruzi In order to study the expression of the foreign ADC gene in T cruzi epimastigotes, we transformed...

Ngày tải lên: 19/02/2014, 07:20

... activation and NAMI-A- induced down regulation of c-myc gene expression NAMI-A inhibits c-myc gene transcription and protein expression To establish whether the decrease in c-myc mRNA expression ... regulates c-myc gene expression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in ... FEBS 2002 NAMI-A affects c-myc gene expression and MEK/ERK phosphorylation (Eur J Biochem 269) 5867 Fig NAMI-A and PD98059 completely inhibits PMA-induced c-myc gene expression (A) Serum-starved...

Ngày tải lên: 21/02/2014, 01:21

... pathways in which one or Gene Expression and Signal Transduction (A) Tryptophan operon 5′ DNA Regulatory gene i Transcription Promoter p Operator o 3′ Gene E Gene D Gene C Gene B Gene A Transcription ... on other genes Other important protooncogenes that encode nuclear transcription factors include MYC and MYB Both phytochrome (see Chap- Gene Expression and Signal Transduction ter 17) and gibberellin ... discussion of eukaryotic gene expression with a brief overview of transcriptional regulation in prokaryotes However, it is now clear that Gene Expression and Signal Transduction gene regulation in...

Ngày tải lên: 07/03/2014, 21:20

... zebrafish SULT1 ST1 and ST2 mRNAs and (B) Western blot analysis of zebrafish SULT1 ST1 protein (A) Detection of zebrafish SULT1 ST1 and ST2 mRNAs in cultured zebrafish cells (lanes and 3) and whole zebrafish ... Bacterial expression and purification of recombinant zebrafish cytosolic STs To amplify the two zebrafish ST cDNAs for subcloning into the prokaryotic expression vector pGEX-2TK, two sets of sense and ... cytosolic STs, several gene families have been categorized within the cytosolic ST gene superfamily Two major gene families among them are the phenol ST (PST) family (designated SULT1) and hydroxysteroid...

Ngày tải lên: 08/03/2014, 02:20

... analysis in WT and TTR KO BMSCs of (B) osteocalcin expression, normalized for the expression of b-actin, at days 3, and 14, (C) osteocalcin expression normalized for the expression of GAPDH and HPRT ... expression Using brain as the positive control of NPY expression, we detected NPY in MC3T3-E1 cells and in both WT and TTR KO BMSCs (Fig 4A) Furthermore, both WT and TTR KO BMSCs on days 3, and ... of WT and TTR KO mice (3 months old) Scale bars: 50 lm B C A C 268 B Fig NPY and PAM expression in bone cells from WT and TTR KO mice (A) NPY RT-PCR analysis in brain, MC3T3-E1 cells, and BMSCs...

Ngày tải lên: 15/03/2014, 09:20

... regulating the gene expression of rate-limiting enzymes of gluconeogenesis and glycogenolysis, including phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), and by regulating ... lipoprotein and several other liver-specific functions, and, most importantly, these functions are stable through passages They are therefore valuable in the study of several hepatic functions and other ... cells, and the ensuing effect on gluconeogenesis and glucose output Our results show that, in the presence of TNFa, insulin-induced inhibition of gluconeogenesis and glucose output is attenuated and...

Ngày tải lên: 16/03/2014, 02:20

... breakdown of ECM and facilitate cell invasion and metastasis Increased expression of uPAR is associated with invasive cancer cell phenotypes and a poor prognosis The ability to decrease uPAR expression ... decreased [9] We have previously cloned the human uPAR gene and characterized its promoter and transcription factors, including two AP-1 and one NF-jB motifs [10–12] We have also found that colon ... uPAR expression in colon cancer invasion and metastasis [13] To develop a potential tool for uPAR gene therapy, we used a catalytic DNA enzyme (DNAzyme) approach The DNAzymes were originally generated...

Ngày tải lên: 16/03/2014, 22:20

... sensitive, and that the pA1lacO-1 promoter and the lacI q1 gene are surrounded by a DNA fragment originating from upstream the gyrA gene and a fragment containing the N-terminal part of the gyrA gene ... digested with the enzymes DraI and EcoRV and a 842-bp fragment containing the DNA region upstream of topA and the topA promoter was isolated and inserted into pUC19 (New England Biolabs) digested with ... were obtained from and used as recommended by New England Biolabs and Boehringer Mannheim Plasmid and ATP, ADP extraction Aliquots (0.8 mL) were removed from cell cultures and placed into an...

Ngày tải lên: 18/03/2014, 01:20

... metabolism and HBV gene expression H Y Kim et al the liver, intestine, kidney and adipose tissue via the regulation of the expression and function of genes involved in bile acid synthesis, uptake and ... metabolism and HBV gene expression Fig The effects of bile acids on HBV gene expression in hepatocyte cell lines (A) Chang liver, HepG2 and Huh7 cells were transfected with the 1.3x HBV-luc construct and ... metabolism and HBV gene expression Fig The effect of AP-1 and C ⁄ EBPs on bile acids-induced HBV gene expression (A) Chang liver cells were cotransfected with AP-1-luc or 3xC ⁄ EBP-luc construct and...

Ngày tải lên: 22/03/2014, 21:21

... of SREBP gene expression is not the initial trigger for the glutamine effects If induction of the SREBP gene expression serves as the initial trigger for the glutamine effects, the gene expression ... in the induction of SREBP-1a gene expression When endogenous Sp1 expression was reduced to  20% of normal with gene- specific small interfering RNA (siRNA), the gene expression of SREBP-1a was significantly ... processed by S1P and S2P after its translocation from ER to the Golgi in response to ER stress Unexpectedly, glutamine suppressed the gene expression of ATF6 (Fig 7A) However, the gene expression of...

Ngày tải lên: 28/03/2014, 22:21

... and P2Y1 and P2Y12 receptor protein expression and functional activity Examination of the effects of specific pharmacological agents (a single agonist and two specific antagonists of P2Y1 and P2Y12 ... Lithuania) Band intensities were determined by densitometric analysis using ingenius genesnap genetools software (Syngene Bioimaging, Cambridge, UK) Only statistically significant immunoblot band intensity ... P2Y receptor subtypes have been cloned and pharmacologically characterized Of these, P2Y2 responds to ATP and UTP, whereas P2Y1 and P2Y12 respond to ADP P2Y1 and P2Y2 receptors are both coupled...

Ngày tải lên: 30/03/2014, 08:20

... (2000) Expression of silicatein and collagen genes in the marine sponge Suberites domuncula is controlled by silicate and myotrophin Eur J Biochem 267, 48784887 Muller WEG (2005) Spatial and temporal ... Obrecht-Pfumio S, Krol A, Thisse B & Lescure A (2003) Spatial and temporal expression patterns of selenoprotein genes during embryogenesis in zebrafish Gene Expr Patterns 3, 525–532 22 Pisera A (2003) Some ... PAGE As shown in Fig 3, the predominant band identified corresponds to a 14-kDa protein (lanes a and b) Weaker bands corresponding to sizes of 35 kDa and 23 kDa were also detected if larger amounts...

Ngày tải lên: 30/03/2014, 20:20

... proinflammatory gene expression in C/EBPb-/- mixed glial cultures Expression of pro-inflammatory (NOS2, IL-1b, IL-6 and TNFa) and anti-inflammatory (IL-4 and TGFb1) genes in C/EBPb+/+ (white bars) and C/EBPb-/- ... the anti-inflammatory genes IL-4 and TGFb1 Absence of C/EBPb results in significant decreases in LPS-induced expression of NOS2 and in LPS+IFNg-induced expression of NOS2 and IL-1b Results are ... proinflammatory gene profiles and neurotoxicity in glial cultures from C/EBPb-null mice Our results show for the first time that absence of C/EBPb attenuates proinflammatory gene expression and abrogates...

Ngày tải lên: 19/06/2014, 22:20

... antiviral, IFN-stimulated genes (ISGs) As specific and sensitive markers we used the ISG56 gene which is induced both by IFNs and by virus infection [38,39] and the MxA gene which is exclusively ... cytokine-inducing viruses activated IP-10 and RANTES expression in HEK 293 cells as expected (Fig 4B, upper and lower panels) Thus, SARS-CoV induces IP-10 gene expression in Caco-2 cells, but not in ... study, and was responsible for drafting and finalizing the manuscript All authors read and approved the final manuscript Acknowledgements We thank Otto Haller for support and helpful comments, and...

Ngày tải lên: 20/06/2014, 01:20

... and identified gene expression patterns altered during the development of chemoresistance Among the genes that are consistently elevated we identify previously known genes such as ABCB1 and genes ... generate novel drug resistant ovarian cancer cell lines independently selected for resistance to cisplatin, doxorubicin or paclitaxel, and we use gene expression profiling to identify genes and ... of gene expression changes This suggested different mechanisms of resistance for the different drugs Comparison of gene expression between sensitive and resistant lines revealed numerous genes...

Ngày tải lên: 20/06/2014, 08:20

... MicroRNAs: genomics, biogenesis, mechanism, and function Cell 2004; 116: 281–297 Tili E, Michaille JJ, Gandhi V, et al miRNAs and their potential for use against cancer and other diseases Future ... in development and function of the immune response In addition we will discus their abnormal patterns of expression associated with cancer and why manipulating their levels of expression is such ... targets of miRNAs Elucidating miRNA-target genes and their mechanism of action has given valid clues in understanding the physiological role of miRNAs in vivo, and is paving the platform for future...

Ngày tải lên: 08/08/2014, 17:20