expression and function of stromal androgen receptor in prostate cancer

... (6.25 µg/test) and streptavidin-FITC in the presence of various amounts of nonbiotinylated JTA009 (thick line: µg/test; thin line: µg/test; thick broken line: 10 µg/test; thin broken line: 25 µg/test) ... tion of human inducible costimulator ligand expression and function J Immunol 2000, 164:4689-4696 Ling V, Wu PW, Miyashiro JS, Marusic S, Finnerty HF, Collins M: Differential expression of inducible ... (phosphate-buffered saline [PBS] containing 0.1% bovine serum albumin and 0.1% sodium azide) and incubated on ice for five minutes with 10 µg purified human immunoglobulin (Cappel, ICN, Aurora, OH, USA) and/ or...

Ngày tải lên: 09/08/2014, 07:20

... Characterization of hHK-1- and endokinins-induced responses in guinea pig airways Contractile effects of hHK-1 and endokinins in isolated guinea pig airways Human hemokinin-1 induced concentration-dependent ... antagonists and the role of hemokinin in T lymphopoiesis Blood 2003, 102(6):2165-2172 16 Weinstock JV: The role of substance P, hemokinin and their receptor in governing mucosal inflammation and granulomatous ... the expression of hHK-1 and the contractile and inflammatory effects of this peptide in human airways Thus, the aims of the present study were to determine the presence of tachykinins, tachykinin...

Ngày tải lên: 12/08/2014, 11:22

... time that expression of CB1 in isolated cortical neurons was affected in both strains of R6 transgenic HD mice It appeared that the amino terminus of mutant huntingtin containing an expanded polyglutamine ... the expression of mutant huntingtin and the exact mechanism by which mutant huntingtin alters the function of such factors This work describing the gene structure of CB1 and its pattern of expression ... levels of mutant huntingtin protein are lower in the R6/1 mice compared to R6/2 [10] and that neuronal intranuclear inclusions (NIIs) containing the human transgene-encoded amino terminus of human...

Ngày tải lên: 16/03/2014, 18:20

... development and function of the immune response In addition we will discus their abnormal patterns of expression associated with cancer and why manipulating their levels of expression is such a promising ... role in the development and maintenance of tolerance and immune T cells Finally, miR-181 family is considered oncogenic Overexpression of miR-181 is observed in breast, pancreas and prostate cancers ... which miR-155 expression is controlled and finding its targets will bring more understanding of the mechanisms of tumor induction by miR-155 76 miR-181 miR-181 is highly expressed in the thymus...

Ngày tải lên: 08/08/2014, 17:20

... expression of JAM-C in a pattern similar to that found in human arthritic tissues Expression of the protein was mainly found in the lining layer and was associated with vessels in the sublining ... tissues We investigated JAM-C expression in human OA and RA synovial samples by IHC using polyclonal antibodies against human JAM-C Interestingly, expression of JAM-C was mainly found in the lining ... given at the base of the tail On day 21, arthritis was induced by intra-articular injection of 100 μg of mBSA in 10 μl of phosphate-buffered saline (PBS) into the left knee joint of mBSA-immunized...

Ngày tải lên: 09/08/2014, 10:20

... MO, USA) Intracellular staining for cytokines was performed using BD cytofix/cytoperm and BD perm/wash reagents with GolgiStop as recommended by BD Pharmingen Intracellular cytokine staining was ... smoke-induced inflammation and expression of chitinases and chitinase-like molecules To investigate the impact of cigarette smoke exposure on chitinase expression, BALB/c, C57BL/6, and CD1 mice ... the inflammatory process and induction of BRP-39 in a model of smoke exposure, we sought to investigate whether IL-13 was essential for the induction of BRP-39 in models of allergic airway inflammation...

Ngày tải lên: 12/08/2014, 13:22

... the incorporation of the pyrimidine analogue BrdU instead of thymidine into the DNA of proliferating cells Five thousand cells in 200 µl of % FBS /DMEM containing 0.8 mg/ml of G418 were seeded in ... tyrosine kinase with four cadherine-like repeats in its extracellular domain and an intracellular tyrosine kinase domain The intracellular domain of Ret consists of 14 tyrosine residues (a short isoform ... signaling receptor for GDNF family ligands proves the existence of a Ret independent signaling pathway NCAM can associates with GFRα1 and promotes highaffinity binding of GDNF and NCAM, resulting in...

Ngày tải lên: 05/10/2015, 22:31

... domain in the N-terminus PX domains occur in a variety of proteins involved in cell signaling, membrane trafficking, and polarity establishment, and function as phosphoinositide-binding modules in ... (F) Expression of Noxo1b, Noxo1c, Nox1, and Noxa1 in various human cell lines: androgen- independent prostate cancer cells (LNCaP), androgen- independent prostate cancer cells (PC3 and DU145), and ... androgenindependent prostate cancer LNCaP cells, and the androgen- independent prostate cancer PC3 and DU145 cells (Fig 2)], indicating that Noxo1c regulates Nox1 in co-operation with Noxa1 in a single cell In...

Ngày tải lên: 19/02/2014, 06:20

... An et al Manduca sexta Spatzle ¨ Introduction A prominent feature of the innate immune systems of insects is the activation of serine proteinase cascade pathways in hemolymph, which function to ... SPE and Easter [31] Injection of either of these M sexta proteinases into larvae stimulated the expression of antimicrobial peptide genes, suggesting that they might function in activation of ... contained a 30 bp insertion in the ¨ ORF, beginning at nucleotide 516 This resulted in insertion of a 10 amino acid segment (TREIDYPETI) and one substitution (Ser fi Gly) at the C-terminal end of...

Ngày tải lên: 06/03/2014, 09:22

... There is increasing evidence that SFs are key players in the pathogenesis of RA by invading and eroding hyaline cartilage SFs, activated locally, produce a variety of cytokines, chemokines and matrix-degrading ... factor, insulin-like growth factor-binding proteins), morphogens, enzymes, numerous cytokines and chemokines, interleukins, and so forth [52] It can be hypothesized that a stringent control of the ... higher expression of HexA compared with HexB in SFs and SM samples This raises the intriguing possibility of intraarticular expression of the rare hexosaminidase S, responsible for degradation of...

Ngày tải lên: 09/08/2014, 14:20

... Up-regulation of XAF1 mRNA and protein by somatostatin and Octreotide in prostate cancer cell lines To examine the regulatory effects of somatostatin and Octreotide on XAF1 mRNA and protein expression, prostate ... somatostatin and Octreotide on XAF1 expression in prostate cancer cell lines We found that somatostatin and Octreotide up-regulated XAF1 mRNA and protein expression in prostate cancer cell lines The ... regulating the activities of phosphotyrosine phosphatases [23] In the current study, we examined XAF1 mRNA and protein expression in four cell lines, and determined regulatory effects of somatostatin...

Ngày tải lên: 10/08/2014, 10:20

... other cytokines induce expression of c-fos and the c-jun oncogene [22-25], we deduced that abnormal expression of c-Fos can be observed in VMC In our experiment, protein expression of c-Fos increased ... signs of cell infiltration in the hearts of uninfected control mice Expression of c-Fos oncogene protein in VMC mice A few c-Fos oncogene protein positive cardiomyocyte nuclei were seen in mice of ... potent inducer of apoptosis in pro-B cells and Ig class-switching B cells c-Fos induced apoptosis is further supported by findings that induction of c-Fos expression is an early event in many...

Ngày tải lên: 12/08/2014, 01:22

... degree of interindividual variability was seen in the staining intensity and number of positive lymphocytes for PRL-1 Central Nervous System Expression of PRL-1 and -2 in the brain was heaviest in ... between the intensity of PRL-1 or PRL-2 staining and the localization of the staining, nor between the localization of staining and any of the clinicopathological parameters examined There were ... longitudinal axis of the digestive tract, increasing from esophagus and cardiac stomach to pyloric stomach and proximal small intestine, then decreasing again in the distal intestine and the...

Ngày tải lên: 24/08/2014, 11:44

... Figure 1.5 Adenosine deamination to inosine by ADAR A Hydrolytic deamination converts adenosine to inosine B Base-pairing of adenosine to uridine (top), base-pairing of inosine to cytidine (bottom) ... regulatory light chain-interacting protein and retinoblastoma-binding protein 1-like miR-155 Overexpressed in CLL, B-cell, Hodgkin’s and Burkitt lymphomas and in human breast cancer SHIP1, CEBPB, ... Analysis of Adenosine-to-Inosine editing of miR376 cluster in gliomas 63 Introduction and aims 63 3.2 Editing analysis of primary miRNAs in gliomas 3.3 Editing analysis of primary miRNAs in glioma...

Ngày tải lên: 10/09/2015, 08:28

... therapeutics for prostate cancer candidate predictor of efficacy in both prostate and breast cancer Finally, the observed sensitivity to dasatinib of prostate cancer cell lines with expression of basal ... degrees of co -expression as well as mutually exclusive expression of AR and CK5 in this data set, reminiscent of the expression pattern of these two genes in basal, intermediate and luminal cells of ... level of uPA expression is enhanced [34] In addition, RNA interference-induced knockdown of uPA inhibits invasion, survival and in vivo tumorigenicity of prostate cancer cells [35] In the clinic,...

Ngày tải lên: 14/08/2014, 08:20

... Brinkmann, A.O & Trapman, J (1997) Functional in vivo interaction between the amino-terminal, transactivation domain and the ligand binding domain of the androgen receptor Biochemistry 36, 1052–1064 ... subdomain AR3)13 in N/C interaction and the role of individual amino acid residues in and flanking the 23 FQNLF27motif in AR16)36 in N/C interaction Yeast protein interaction assays indicated that AR3)13 ... modulates androgen receptor N/C interaction (A) Interaction of AR.NTDwt and N-terminal deletion mutants with AR.LBD in the presence of lM DHT in the yeast protein interaction system In each experiment...

Ngày tải lên: 17/03/2014, 10:20

... and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across a range of ... v-KIND derivatives: v-KIND, full-length v-KIND; DKIND1, deletion of KIND1; DKIND2, deletion of KIND2; DKIND1 + 2, deletion of both KIND1 and KIND2; DRasN, deletion of RasN; DGEF, deletion of ... in the KIND2 domain of v-KIND contain the core MAP2 binding module To identify the core MAP2-binding site within the KIND2 domain (amino acids 456–620), we analyzed five EGFP-fused KIND2 domain...

Ngày tải lên: 14/02/2014, 19:20

... post-injury and remained elevated for almost a month [19,20] The increase in GIF expression in response to these different types of brain injury was often observed in glial cells accumulating ... important insights into the functional roles of this protein in the brain, and in particular its ability to inhibit neurite outgrowth (as described above) The functional role of GIF synthesis and secretion ... against Ab pathogenesis and neurotoxicity in AD (Fig 2) Conclusion The discovery of a brain-specific MT isoform, GIF, in 1991, sparked considerable interest in understanding the role of all MTs in...

Ngày tải lên: 16/02/2014, 15:20

... of participate in the formation of EpI-resistant a7 receptors, or an ACh-binding protein reveals the ligand-binding domain of that the functional properties of the nAChR are modified in nicotinic ... ligand-binding domain of the a6 subunit and the transmembrane and intracellular domains of the a3 subunit was used in this study PIA selectively blocks rat and human nAChRs that contain a6b2 interfaces ... domain of each subunit contributes to the formation of the hydrophilic pore ACh binding protein has structural and functional homology to the extracellular ligand binding domain of the nAChR, and...

Ngày tải lên: 19/02/2014, 12:20

... Thr54fiMet121 in CfAFP-501 (B) Overlap of isoforms 337 and 501 using the main chain of residues 4–33 in both proteins show that despite the > 10-fold difference in TH activity, fish and insect AFPs ... structure and PDB code shown below each panel (A) Overlap of isoform 337 with the structure of the longer isoform CfAFP-501 using the main chain of residues Thr23fiAsn90 in isoform 337 and residues ... arrangement of polar groups that could bind ice The inability of researchers to propose a consistent model explaining the type III AFP/ ice-binding in terms of hydrogen bonding has led to the proposal of...

Ngày tải lên: 19/02/2014, 16:20