characterization of isolated ck cells

... observe EpCAM +CK- cells (0/15), whereas we observed the presence of EpCAM -CK+ cells in samples (2/15 = 13%) when cells were enriched by CD45 depletion The median number of CK+ cells was 2/ 10 ... the presence of single EpCAM or CK positive cells In blood samples, found to be negative for presence of EpCAM +CK+ CD45- cells, EpCAM -CK +CD45- cells were detected in 38% (14/37) of peripheral ... median of the number of these cells was (range: 1-43)/10 mL blood EpCAM+CKCD45- cells were detected in only two cases The detection rate of EpCAM -CK+ CD45- cells was significantly higher than of EpCAM +CK- CD45...

Ngày tải lên: 18/06/2014, 19:20

... observe EpCAM +CK- cells (0/15), whereas we observed the presence of EpCAM -CK+ cells in samples (2/15 = 13%) when cells were enriched by CD45 depletion The median number of CK+ cells was 2/ 10 ... the presence of single EpCAM or CK positive cells In blood samples, found to be negative for presence of EpCAM +CK+ CD45- cells, EpCAM -CK +CD45- cells were detected in 38% (14/37) of peripheral ... median of the number of these cells was (range: 1-43)/10 mL blood EpCAM+CKCD45- cells were detected in only two cases The detection rate of EpCAM -CK+ CD45- cells was significantly higher than of EpCAM +CK- CD45...

Ngày tải lên: 20/06/2014, 03:20

... Identification of IL-4-producing cells of MLN in 4get mice 108 Figure 4.2.3 Kinetics of IL-4-eGFP+ CD4 T cells in MLN of 4get mice 110 Figure 4.2.4 Kinetics of IL-4-eGFP+ innate cells in MLN of 4get ... family of innate effector cells involved in asthma are natural killer T cells (NKT cells) with a unique feature of both classical T cells and NK cells Particularly, invariant NKT cells (iNKT cells) ... Isolation of CD4 T cells by magnetic separation 60 2.3.2 Adoptive transfer of CD4 T cells 61 2.3.3 Sorting of CD4 T cells 61 2.3.4 CFSE labeling of CD4 T cells ...

Ngày tải lên: 10/09/2015, 09:04

... (Yoto et al., 1996) The sensitivity of this PCR assay allowed detection of as few as 10 copies of B19 genome In addition, we sequenced the B19 isolates consisted of 4561 bp covering NS1, VP1 and ... Discussion The etiology of hepatitis remains obscure in 3-10% of cases in Europe [5] and the United States [6] and up to 30% of cases in Asia [7, 8] Particularly, the cause of fulminant hepatitis ... National Study Group of Fulminant Hepatitis Clinical epidemiology of fulminant hepatitis in Japan before the substantial introduction of liver transplantation: an analysis of 1309 cases in a 15-year...

Ngày tải lên: 26/10/2012, 10:03

... compartment (mol.L-1) VB is the volume of right reservoir (cm3) A and L are the membrane cross-sectional area and thickness, respectively α is the slope of the linear plot of methanol concentration in ... hydrophobic property of PS polymer promoted the formation of these polymer aggregates As can be seen, the thermally induced polymerization of styrene, as well as the blending of PS and SPS polymers, ... uptake of the PVDF membrane decreasing from 22.05% to 14.31%, which can explain the hydrophobic property of PS polymer However, the water uptake of PVDF-SPS membrane is larger than that of the...

Ngày tải lên: 05/09/2013, 16:11

... tumour cells A M Luciani et al For 1D spectra, deconvolution of spectra of the type shown in Fig 1AÂ, with ML signals of a very low intensity, was performed as a rst step Deconvolution of spectra of ... Example of the deconvolution pattern of the methylene region in the 1D 1H NMR spectra of MCF-7 cells (A); integration regions for selected cross peaks in the 2D 1H NMR spectra of MCF-7 cells (B) ... pattern of the same region (C) and of the reference signal (CÂ) (p.p.m.) Table Mean values of parameters d (p.p.m.), Dm (Hz) and IR after deconvolution of 1D spectra from PCA extracts of HeLa cells...

Ngày tải lên: 18/02/2014, 13:20

... 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28), respectively These data showed that VGE fusion protein of EcR ... switch The current versions of bipartite EcR gene switch have very low background activity of reporter gene in the absence of ligand and Functional characterization of EcR gene switches are activated ... understanding of the molecular mechanism of EcR gene switches in mammalian cells and development of an efficient EcR gene switch that showed low background activity in the absence of ligand and...

Ngày tải lên: 16/03/2014, 12:20

... log of the observed first-order rate kinetics of inactivation constant against log of EDTA concentration n, the slope of the plot, was calculated as 0.44 and estimates the number of molecules of ... of APN by detergents In order to evaluate the solubilizing efficiency of detergents, samples of 200 lL midgut homogenate at a concentration of 13 guts per mL (which contains  50 lg protein) of ... the incubation mixture of ConA with APN at a concentration of A B C Fig Inactivation of A pisum APN by EDTA at 37 °C Reaction mixtures contained different concentrations of EDTA in 100 mM Tris...

Ngày tải lên: 16/03/2014, 12:20

... of the formation of intracellular organelles and without alteration of the morphology of vacuolar compartments, resulting in the profound inhibition of the endosomal ⁄ lysosomal degradation of ... processing of wildtype cathepsin E, the transfected cells were labeled 220 A B Fig Immunoblot analysis of the cell extract of HEK-293T cells expressing wild-type cathepsin E HEK-293T cells were ... Pulse–chase analysis of the mutants lacking the propeptide and ER-retention sequence (A) Schematic representation of the structures of the mutants lacking the propeptide and most of the ER-retention...

Ngày tải lên: 16/03/2014, 14:20

... characteristics of the fabricated SiNWs solar cell under dark and light conditions the thickness of Si base substrate more thinly For improvement of conversion efficiency of the SiNWs solar cells, the ... Therefore, further reduction of the reflectance will be expected from the optimization of the synthesis conditions of SiNWs For analysis of the electrical properties, the I–V curve of the fabricated SiNWs ... at λ b 700 nm [20] The difference of the reflectance might be the effect of synthesis conditions, such as thickness of metal film and SiH4 gas flow The reduction of reflectance is very helpful to...

Ngày tải lên: 16/03/2014, 15:12

... Rutter J, Reick M, Wu LC & McKnight SL (2001) Regulation of Clock and NPAS2 DNA Binding by the Redox State of NAD Cofactors Science 293, 510–514 Reppert SM & Weaver DR (2002) Coordination of circadian ... alignments of PAS-B and PAS-A of NPAS2, PAS-B of HIF2a, a heme-binding PAS domain of BJ FixL and PAS-A of Ec DOS Amino acids at 266, 289, 300, 302, 329, and 335 with bold H of PAS-B of NPAS2 were ... differences in the method used to prepare the isolated domains Dioum et al prepared the isolated domains by denaturation of an insoluble suspension of E coli cells overexpressing the protein and then...

Ngày tải lên: 16/03/2014, 22:20

... RhoCT19N and RhoCQ14L cDNA were obtained by quickchange punctual mutations of the wt cDNA of RhoC in pPC86 (kind gift of P Chevray of the University of Texas, Houston, TX, USA and D Nathans from ... in most cells, suggestive of a translocation of p76RBE protein to a vesicular compartment due to the presence of activated RhoB (Fig 4D) Regulation of p76RBE mRNA in vitro in thyroid cells As ... Genome Browser) Specific association of p76RBE with GTP-bound form of RhoB The presence of an HR1 domain and the high degree of homology with Rhophilin in the NH2 part of the protein suggested that...

Ngày tải lên: 23/03/2014, 21:20

... h resulted in 95% of cells arrested in G0 ⁄ G1 phases, with less than 5% of the cells in other cell cycle phases Figure shows that the addition of fresh serum-free medium to cells starved for ... number of cells in the S phase On the other hand, addition of serum-containing medium (Fig 3, M+) almost completely restored the control pattern of the cell cycle phases, with  70% of cells in ... staining on the whole cell surface in most of the cells (green, G); some cells still have vinculin staining similar to starved cells (arrow) (D, H) Cells cultivated for 96 h in serum-free MEM...

Ngày tải lên: 30/03/2014, 08:20

... Rutter J, Reick M, Wu LC & McKnight SL (2001) Regulation of Clock and NPAS2 DNA binding by the redox state of NAD cofactors Science 293, 510–514 13 Reick M, Garcia JA, Dudley C & McKnight SL (2001) ... FEBS 2531 Characterization of bHLH-PAS-A of NPAS2 Y Mukaiyama et al Table Resonance Raman spectra of Fe(II)–CO complexes of the basic helix–loop–helix (bHLH)-PAS-A and PAS-A domains of neuronal ... spectra of CO adducts of heme proteins provide valuable information about the heme pocket [17,32] Low-frequency and high-frequency regions of the resonance Raman spectra of the Fe(II)–CO complex of...

Ngày tải lên: 30/03/2014, 11:20

... function of tumor-educated myeloid cells was measured by their ability to inhibit the proliferation of autologous T cells in the following Suppression Assay: T cells isolated from 30 mL of PBMC ... (CD33+) cells were then isolated, checked for viability, and tested for suppressive function by coculture with fresh, autologous T cells in the presence of T cell stimuli Use of irradiated tumor cells ... shown from Suppression Assays of CD33+ or CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cells from co-culture (p

Ngày tải lên: 18/06/2014, 19:20

... Phylogenetic analysis of the N gene sequences of wild-type MVs isolated in Turkey Phylogenetic analysis of the N gene sequences of wild-type MVs isolated in Turkey Sequences of the Turkish viruses ... from of the IgM negative cases were taken days after rash onset when the sensitivity of IgM detection is low Comparison of the N gene sequences of the Turkish viruses with the sequences of the ... sequences of 21 of these MV isolates were identical, though they came from different regions of Turkey Although the Turkish viruses were clearly in genotype D6, the sequences of the more recently isolated...

Ngày tải lên: 19/06/2014, 08:20

... Journal of Occupational Medicine and Toxicology 2007, 2:1 Background A variety of isolated and perfused kidney models has been used for the study of renal functional parameters ... Figure The U/P quotient of creatinine U/Pcrea versus of creatinine; for isolated kidney water reabsorption) The U/P quotient of creatinine U/Pcrea versus urine flow VU for isolated kidney experimental ... of the isolated kidney In this respect, considering the nephron's handling of substrates, the tubular reabsorption of sodium is a further prominent mechanism and the fractional reabsorption of...

Ngày tải lên: 20/06/2014, 00:20

... A/black-neckedgrebe/Germany/R1393/20072 A/chicken/Afghanistan/1573-65/2006 91 A/chicken/Afghanistan/1573-47/2006 A/chicken/Afghanistan/1573-92/2006 A/chicken/Afghanistan/1207/2006 A/chicken/Viet ... 98 A/chicken/Sudan/2115-10/2006 A/chicken/Sudan/2115-12/2006 96 A/chicken/Sudan/1784-10/2006 A/Indonesia/CDC699/2006 A/duck/Vietnam/1/2005 44 97 60 85 A/chicken/Thailand/PC-168/2006 A/chicken/Thailand/PC-170/2006 ... olor/Astrakhan/Ast05-2-3/2005 A/chicken/Nigeria/BA210/2006 A/chicken/Nigeria/957-20/2006 A/chicken/Nigeria/1047-54/2006 A/chicken/Nigeria/1047-34/2006 A/chicken/Nigeria/1047-30/2006 77 A/ostrich/Nigeria/1047-25/2006...

Ngày tải lên: 20/06/2014, 01:20

... Phylogenetic analysis of the N gene sequences of wild-type MVs isolated in Turkey Phylogenetic analysis of the N gene sequences of wild-type MVs isolated in Turkey Sequences of the Turkish viruses ... from of the IgM negative cases were taken days after rash onset when the sensitivity of IgM detection is low Comparison of the N gene sequences of the Turkish viruses with the sequences of the ... sequences of 21 of these MV isolates were identical, though they came from different regions of Turkey Although the Turkish viruses were clearly in genotype D6, the sequences of the more recently isolated...

Ngày tải lên: 20/06/2014, 04:20