cellular+applications+requiring+in+vivo+self+assembly

... of the resulting PANI/magnetite nanorods prepared by in situ self- assembly method As shown in Fig 2A, PANI/magnetite nanorods has an unique nanorods structure in shape, ca 80 nm in diameter and ... can be further increased by increasing the thickness of magnetite coating Summary In summary, PANI/magnetite nanorod composites were synthesized through DBSA micelle-assisted self- assembly method ... Materials Results and discussion Aniline was obtained from Beijing Xingjin Chemical Factory, and was distilled at a reduced pressure before use DBSA (Anyang Xingya Chem Ltd.) was used as the surfactant...

Ngày tải lên: 20/03/2014, 13:04

... the protein The self- cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self- cleavage was also observed in all investigated bacterial SPase I including enzymes ... the selfcleavage is happening in vivo within the bacterial cells In the current study, we have shown that phospholipid enhances the activity of self- cleavage, and self- cleavage results in the inactivation ... explain why self- cleavage is happening in vivo, one speculation is that it may be involved in regulating the activity of the enzyme It is not difficult to imagine that bacteria may secrete proteins...

Ngày tải lên: 23/03/2014, 21:21

... Phenylalanine G Gly Glycine H His Histidine I Ile Isoleucine K Lys Lysine L Leu Leucine M Met Methionine N Asn Asparagine P Pro Proline Q Gln Glutamine R Arg Arginine S Ser Serine T Thr Threonine V ... peptide/protein having an N- terminal cysteine and a peptide/protein containing a C-terminal thioester.[16] In the case of expressed protein ligation (EPL), intein-mediated splicing is used to ... against PTP1B binding peptide 2.3.2 Microplate investigation on the binding of PTP1B-ABDF 54 against c-Src catalytic domains 2.3.3 Microplate investigation on the binding of PTP1B-ABDF 59 against...

Ngày tải lên: 04/10/2015, 15:45

... particularly useful in diagnosing incident cancers in otherwise normal tissues Indeed, this approach discriminates normal and neoplastic tissues in various cancer types, including CLL, breast cancer, ... of essential proteins responsible for accelerating the cell cycle, including cyclin D and retinoblastoma [56,57] Moreover, alterations in the machinery responsible for processing miRNA contributes ... RNA-binding-domain protein that is a homologue of the HIV transactivating response RNA-binding protein (TRBP) The functional strand of the miRNA duplex guides the RNA-induced silencing complex (RISC)...

Ngày tải lên: 16/03/2014, 01:20

... protein’s ability to support cell growth indicates that this Cys, which is conserved in Gpi3/Pig-A proteins although not in other EX7E-containing proteins, is not important for enzyme activity in ... E297D-expressing strains were temperature-sensitive Secondly, membranes containing Gpi3p-E297D had no detectable GlcNAc-PI synthetic activity, whereas those containing Gpi3p-E289D retained in vitro ... 14C signal in each sample migrating at the position of [14C]GlcNAc-PI on the chromatogram in (C) were quantified using a Phosphorimager and plotted as relative intensities in vivo findings not support...

Ngày tải lên: 30/03/2014, 20:20

... stress in an occupational setting such as those in the automotive assembly industry Therefore, in the present study, we evaluate the effectiveness of a short duration SMT in reducing self- perceived ... 15 minutes warming up stretching exercises [13] This was followed by low intensity walking and jogging routines [16] Participants were then taken through a series of rhythmic dancing and running ... relaxation training (10 minutes) on Japanese female medical co-workers indicated that salivary immunoglobulin A (s-IgA) levels in the relaxation group increased after relaxation training compared...

Ngày tải lên: 20/06/2014, 00:20

... salts in solution: indeed, the marginal staining by rhodamine suggests that the proportion of depolymerized tubulin in the film is low Fluorescence microscopy of the latter revealed that it contained ... produced interesting results, even though the resulting combing patterns were difficult to reproduce and strongly inhomogeneous across the surface (see Fig S2 in supporting information) Finally, ... dilution in water, it may also comprise depolymerized tubulin Interestingly, this film seems to have occasionally been pinned during drying by the individual MTs, (this is clearly highlighted in Fig...

Ngày tải lên: 22/06/2014, 18:20

... salts in solution: indeed, the marginal staining by rhodamine suggests that the proportion of depolymerized tubulin in the film is low Fluorescence microscopy of the latter revealed that it contained ... produced interesting results, even though the resulting combing patterns were difficult to reproduce and strongly inhomogeneous across the surface (see Fig S2 in supporting information) Finally, ... dilution in water, it may also comprise depolymerized tubulin Interestingly, this film seems to have occasionally been pinned during drying by the individual MTs, (this is clearly highlighted in Fig...

Ngày tải lên: 22/06/2014, 22:20

... sensoring the in vivo molecular environment surrounding the nanoparticles and tracing the in vivo biodistribution of CLIO in liver, spleen and kidneys [38] Obviously, due to the excellent in vivo ... years, NIRF labeling has played an increasingly important role in in vivo studies [28-33] Therefore, this study investigated the applicability of the IRDy800CW-MNPs in this field The in vivo studies ... biodistribution is independent of the routes of administration, such as intravenous injection [13-15,18,53,75,78,79], intraperitoneal injection [26], intratracheally instillation [77], and inhalation...

Ngày tải lên: 11/08/2014, 00:22

... 7) In vivo morphometric and volumetric findings were found to correlate well with prior ex vivo studies in vertebrate livers (Table 6), though in vivo findings see marginally higher hepatocellular ... captures, illustrating resorufin fluorescence (red) in the liver (L) and gall bladder (GB) (C1 and C2) In vivo imaging of region of interest in frame B3 (white square) showing in vivo phenotype of ... good for investigating blood to bile transport, IHBPs, EHBPs and intestinal lumen 10–50 μM 7-Ethoxyresorufin DMSO 30/45 In vivo imaging of CYP1A activity Uptake via gill, in vivo metabolism in gill...

Ngày tải lên: 13/08/2014, 13:20

... Kang in the group I would like to thank the internal and external examiners for spending precious time in examining my thesis and providing valuable comments I am thankful to A/Prof Yang Kun-Lin ... micelle-p53 encoding plasmid.14 In another study, different combinations of alanine and phenylalanine in FA32 (while keeping the same hydrophilic chain) were synthesized to examine the effect of ... explore the self- assembly of peptides and specific applications Microscopic insights are indispensable for elucidating self- assembly at a molecular level However, few simulation/modeling studies...

Ngày tải lên: 09/09/2015, 08:13

... “open” or “closed” integrin conformation and thus regulates ligand binding The extracellular binding to integrins The extracellular binding can stimulate integrins to become clustered in the panel ... assembly of actin filaments It in turn promotes more integrin clustering, thus enhancing the matrix binding and organization by integrins in a positive feedback system As a result, ECM proteins, ... medicine was introduced in 1999, with the aim of combining together tissue engineering and cellular therapeutics (Fishman et al 2011) In most cases, both regenerative medicine and tissue engineering...

Ngày tải lên: 09/09/2015, 18:57

... characterized by insulin resistance and insulin secretion deficiency At present, there is no a single medication which treats type diabetes by improving both insulin resistance and insulin secretion ... glycine-rich linker is flexible, which allows the specific engineering of hinge regions into proteins to achieve desired functional motions [17] We used a glycine-rich short peptide including 15 amino ... fusion protein in inclusion body was refolded in urea gradient refolding buffer And then, the refolded protein was incubated with enterokinase to remove the His-tag The fusion protein without...

Ngày tải lên: 25/10/2012, 11:10

... Micelles 6.4 Self- Assembly at the Liquid–Solid Interface 6.5 Self- Assembly at the Liquid–Liquid Interface 6.6 Self- Assembly at the Gas–Solid Interface 6.7 Interface-Induced Chiral Self- Assembly References ... µm 100 µm cm atomic self- assembly molecular self- assembly colloidal (mesoscopic) self- assembly biological self- assembly interfacial self- assembly Figure 1.1 Classification of self- assemblies based ... building units for this initial self- assembly, issue will be discussed in and the DNA is the primary self- aggregate detail in Chapter DNA is then self- assembled into a primary structure of protein...

Ngày tải lên: 15/11/2012, 10:12

... anti-inflammatory activity is measured by the model of carrageenin-induced edema in hind paw of the rat Results: 10 Polyoxychalcones were obtained with good yields and the results of in vivo ... affect to the in vivo anti-inflammatory activity of tested chalcones The polyoxychalcone which possessing a hydroxy group at position C2 of A ring and at least one group methoxy in B may be consider ... nhóm methoxy vòng B làm tăng tác dụng kháng viêm in vitro in vivo chalcon - Nhóm 2’-hydroxy có ảnh hưởng định đến tác dụng kháng viêm in vitro in vivo chalcon Tăng tác động kháng viêm cho chalcon...

Ngày tải lên: 15/11/2012, 14:03

... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson, ... i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma...

Ngày tải lên: 23/04/2013, 21:38

... triphosphate BAP : 6-Benzylaminopurine CV : Correlation of Variants D : DMSO : Dimethyl sulphoxide EMS : Ethyl methane sulphonate G : G ch Ki : Kinetin (6-Furfurylaminopurine) LSD : Least Significant ... tớnh nụng sinh h c c a m t s dũng hoa ng Ti n (Gerbera jamesonii) t bi n t o c nuụi c y in vitro v in vivo 1.2 M c ớch v yờu c u c a ti 1.2.1 M c ớch ỏnh giỏ c cỏc c tớnh nụng sinh h c c ... 47 Bi u 6: Kh nng sinh tr ng v nhõn nhanh c a cỏc d ng ch i ng Ti n t bi n in vitro (gi ng T) 50 Bi u 7: Kh nng sinh tr ng v nhõn nhanh c a cỏc d ng ch i ng Ti n t bi n in vitro (gi ng D)...

Ngày tải lên: 14/12/2013, 16:09

... prostaglandin D2 in adipogenesis in vivo Y Fujitani et al indicate that overexpression of H-PGDS increases insulin sensitivity in vivo Adipocyte differentiation ex vivo Finally, we examined whether ... improvement in peripheral insulin resistance through the activation of PPARc in WAT, it is possible that PGD2 stimulates pancreatic islets to increase insulin secretion Indeed, serum insulin levels ... were increased after treatment with thiazolidinediones in diabetic mice through regulation of insulin production in pancreatic islet cells [39–41] Thus, the increased insulin level seen in H-PGDS...

Ngày tải lên: 16/02/2014, 09:20