cells for tissue engineering

... CULTURE OF CELLS FOR TISSUE ENGINEERING Culture of Specialized Cells Series Editor R Ian Freshney CULTURE OF CELLS FOR TISSUE ENGINEERING Gordana Vunjak-Novakovic and ... of Cells for Tissue Engineering is a new volume in the John Wiley series Culture of Specialized Cells, with focus on procedures for obtaining, manipulating, and using cell sources for tissue engineering ... effort to provide a user-friendly reference for sourcing, characterization, and use of cells for tissue engineering, for researchers with a variety of backgrounds (including basic science, engineering, ...

Ngày tải lên: 22/03/2014, 15:20

... vivo tissue engineering unless specified An essential requirement for tissue engineering is to provide cells with a favorable environment for tissue regeneration In the case of in vitro tissue engineering, ... attachment, the barrier for cells to retain the site for tissue regeneration, the guide for cells to create a tissue giving the contour of the regenerated tissue, the carrier for the sustained release ... capillary formation is impossible in the in vitro tissue engineering This may be the reason for very limited applications of in vitro tissue engineering mostly to epidermal production Tissue engineering...

Ngày tải lên: 25/10/2013, 08:20

... scaffolds, which are used for framework and initial support for the cells to attach, proliferate and differentiate, and form an extracellular matrix, is one area of tissue engineering [2] The goal ... of three-dimensional scaffolds for tissue engineering Chem Eng Res Des 2007, 85:1051-1064 Guarino V, Cause F, Ambrosio L: Bioactive scaffolds for bone and ligament tissue Expert Rev Med Dev 2007, ... phosphate for tissue engineering scaffold Sung-Min Kim1, Soon-Aei Yi1, Seong-Ho Choi2, Kwang-Mahn Kim1, and Yong-Keun Lee*1 Department and Research Institute of Dental Biomaterials and Bioengineering,...

Ngày tải lên: 21/06/2014, 17:20

... with engineered tissues and scaffolds containing endothelial cells or endothelial progenitor cells This proposed approach is novel for improving neovascularization in tissue engineering 1.3 Regulation ... reduce the formation of a fibrotic sheath around an implanted tissue engineered construct would therefore be of great benefit in tissue engineering 20 1.11 Collagen Biosynthesis Collagen forms the ... Healing Regenerative medicine and tissue engineering often inevitably require surgery for implantation of tissue engineered constructs Surgery creates wounds, therefore provoking a wound healing...

Ngày tải lên: 11/09/2015, 14:29

... nutrients to cells in the interior of a tissue construct; while fibrosis may occur as a result of a foreign body reaction to encapsulate the tissue construct with fibrous collagenous tissue Prolyl ... Biosynthesis in vitro and in vivo 99 4.8 Applications in Tissue Engineering: Pre-vascularizing PLLA Scaffolds 101 4.9 Advantages of Using PHi in Tissue Engineering 105 CONCLUSIONS 108 REFERENCES 111 v ... approach to improve vascularization of tissue engineered constructs is also novel Coupled with anti-fibrotic properties, PHi may be a potent aid for tissue engineering, by tackling both the problem...

Ngày tải lên: 12/09/2015, 08:18

... materials for tissue engineering applications In tissue engineering, a temporary matrix is required to serve as an adhesive substrate for the implanted cells and a physical support to guide the formation ... and practical applications for this material Among the potential explorations identified for chitin based materials is in the emerging field of tissue engineering Tissue engineering provides an ... These matrixes have the potential to provide a structural framework for cells and facilitate formation of new tissues in tissue engineering The second part of the thesis will investigate the chemical...

Ngày tải lên: 17/09/2015, 17:20

... Tissue Engineering Two-dimensional matrices, in the form of thin films, have special applications in tissue engineering The earliest and most successful application of 2D matrices in tissue engineering ... ability to fabricate 3D free-form scaffolds and the built scaffolds have potential for use in tissue engineering applications Keywords: Rapid Prototyping, 3D Scaffold, Tissue Engineering, Chitosan, ... Introduction 1.1 Tissue Engineering The need to produce tissues and organs for organ transplant due to the acute shortage of tissues and organs [Mooney and Mikos, 1999], and a possible mismatch of tissue...

Ngày tải lên: 30/09/2015, 13:49

... application in tissue engineering Microassembly technique has shown much potential in facilitating tissue regeneration tasks In this work, an automatic system is developed for building 3D tissue engineering ... implementation of force control, especially the control of the assembly force or the insertion force This thesis develops techniques for efficient z-axis microassembly based on force control of ... the proliferation and spread of seeded cells in vitro and in vivo (Zhang, 2005) For example, matrix-producing connective tissue cells or anchorage-dependent cells taken from a patient can be seeded...

Ngày tải lên: 30/09/2015, 14:24

... CellTracker™Blue x Chapter Introduction 1.1 Tissue engineering 1.1.1 Overview of tissue engineering In the field of tissue engineering, principles of engineering and life sciences are integrated ... 109] In order for spheroid to form in this way, different substratums are required for different types of cells For example, primary hepatocytes spheroid can be formed by culturing cells on positively ... combining principles from tissue engineering, cell surface engineering and chemistry Instead of using classical tissue engineering approach, which involves seeding cells into polymer scaffold...

Ngày tải lên: 05/10/2015, 21:23

... insight into the field of nanotechnology and tissue engineering A/Prof Phan Toan Thang, from the Department of Surgery (NUS / Division of Bioengineering), for providing their expertise knowledge and ... focuses mainly on using nanometer scale fibers for biomedical applications This is especially so in fabrication of the nanofibrous scaffold for tissue engineering (TE) This technique produces non-woven ... fibroblast cells are expanded and seeded onto a bioabsorbable scaffold under aseptic conditions The cells are tested throughout the process for bacteria, viruses, fungi, and mycoplasma The cells attach...

Ngày tải lên: 26/11/2015, 22:39

... fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells ... hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells for their ... be determined for functional cartilage tissue engineering 2.4 Cartilage formation and regeneration using ESCs To date, there is only one study that has utilized hESC-derived cells for cartilage...

Ngày tải lên: 11/09/2015, 09:57

... Hematopoietic stem cells and marrow stromal cells As hematopoietic stem cells (HSCs) and marrow stromal cells (also known as mesenchymal stem cells) (MSCs) are more accessible than other cells mentioned ... Olfactory ensheathing cells (OECs) Olfactory ensheathing cells (OECs) are glial cells ensheathing the axons of the olfactory receptor neurons These cells have properties of both Schwann cells and astrocytes, ... and Bunge [31] for review) The core approach of tissue engineering consists of provision of an interactive environment between cells, scaffolds and bioactive molecules to promote tissue repair...

Ngày tải lên: 19/06/2014, 10:20

... stem/progenitor cells Storage of putative stem/progenitor cells is also an attractive research target in tissue engineering Collection of cells for tissue engineering is less invasive than conventional tissue ... Skin Cells for Optimal Skin Reconstruction by Tissue Engineering 195 Moulin VJ, Mayrand D, Laforce-Lavoie A, Larochelle S and Genest H Chapter Mesenchymal Stem Cells for Cell Therapy and Tissue ... improved before widespread practical use of tissue engineering in hospitals or clinics In this chapter, the current status of cell culture media used for clinical tissue engineering and the need for...

Ngày tải lên: 28/06/2014, 05:20

... our remaining stem cells for tissue regeneration and repair? In conclusion, MSCs derived from adult tissue present an exciting progenitor cell source for applications of tissue engineering and regenerative ... stem cells To seriously consider the applications of MSCs for regeneration and tissue engineering, two key fundamental questions regarding these cells must be addressed: what exactly are these cells? ... responsible for such phenotypic conversion remain unknown, these findings hold much promise for the future of tissue engineering and regeneration [134] Mesenchymal stem cells versus embryonic stem cells...

Ngày tải lên: 09/08/2014, 01:21

... Therefore, the exogenous stem/progenitor cells, such as embryonic stem cells (ESCs), bone marrowor fat-derived mesenchymal stem cells (MSCs), and recently amniotic fluid stem/progenitor cells, ... development of lung tissue constructs composed of primary lung cells has been developed For example, the development of a tissueengineered model of human bronchiole consisting of primary cells and designed ... (EGF), connective tissue growth factor, FGFs, and their receptors) [4] The epithelial cells and inflammatory cells of the airways also release inflammatory mediators such as transforming growth...

Ngày tải lên: 10/08/2014, 05:21

... pulpal involvement) The lack of any enamel forming cells in the enamel of fully developed erupted teeth precludes the potential for cell-based approaches for enamel regeneration In contrast, the ... highly vascular and innervated pulpal tissue, forming a tightlyregulated "dentin-pulp complex" During primary tooth formation, dentin is produced by odontoblastic cells located within the pulp Following ... mesenchymal progenitor cells into odontoblast-like cells [15] B Conventional techniques for inducing pulpal repair Calcium hydroxide has long been the "gold standard" for pulp capping [16] Its...

Ngày tải lên: 11/08/2014, 23:22

... Bioreactor Stimulation of Mesenchymal Stem Cells and Endothelial Progenitor Cells Cocultures in 3D Honeycomb Scaffolds for Vascularised Bone Tissue Engineering Tissue Engineering Part (A) 2012 Oct 26 doi:10.1089/ten.TEA.2012.0187 ... SH Teoh Cellular interactions of endothelial progenitor cells and mesenchymal stem cells for vascularised bone tissue engineering Tissue Engineering and Regenerative Medicine International Society ... Biochemical Cues .26 2.6 Bone Tissue Engineering 30 2.6.1 Four-Stage Bone Formation in Bone Tissue Engineering 30 2.6.2 Strategies in Bone Tissue Engineering .32 xi 2.6.2.1...

Ngày tải lên: 09/09/2015, 10:16

... Frozen cells were thawed when the cells were required for 31 experiments Since most cells were frozen after the second passage, cells used for the experiments were mostly at passage number four Cells ... border enzyme GGT, cells were incubated with phenol red-free media For measuring GGT activity in static culture, the cells were cultivated for at least days after seeding Cells had formed a confluent ... soluble factors Endothelial cells have also been shown to impact the performance of renal proximal tubule cells in vitro For instance, bovine aortic macrovascular cells regulate PTC sodium 26...

Ngày tải lên: 09/09/2015, 10:19

... neointimal hyperplasia [84, 106] 2.3 Vascular Tissue Engineering (VTE) Vascular tissue engineering (VTE) is an interdisciplinary field involving the principles of engineering and life sciences to develop ... TCP Tissue Culture Plate TEBV Tissue Engineered Blood Vessel TGF-β1 Transforming Growth Factor Beta UV Ultraviolet UV-Vis Ultraviolet-Visible vSMC Vascular Smooth Muscle Cell VTE Vascular Tissue ... development of the vSMCs and connective tissue cells through signalling As ECs are in direct contact with the blood, they perform a critical role in all aspects of tissue homeostasis [38] Quiescent...

Ngày tải lên: 10/09/2015, 09:08