capillary electrophoresis mass spectrometry ce ms

... interferences may be formed, as illustrated in Table For quadrupole ICP -MS (ICP-QMS) the established and most effective method of reducing polyatomic interferences in high purity matrices is the ... pressure of even the most volatile solvent (such as acetone) is sufficiently reduced to allow stable plasma operation Removal of carbon The presence of high levels of organic solvent in the sample ... “tongue”, visible in the central channel of the plasma, is seen to stop well before sample cone orifice This indicates that the organic matrix has been decomposed, see Figure Once the optimum oxygen...

Ngày tải lên: 05/03/2014, 21:20

... T-Wave thứ hai: cho MS2 TOF: Lựa chọn V mode/ W mode GC -MS Tách GC Xác định MS Có thư viện phổ chuẩn Interface Mass spectrometer acts as a detector for GC LC -MS • Tách LC Xác định MS • Khó khăn: loại ... nhận số lượng chúng tỷ lệ với cường độ tín hiệu Máy MS tứ cực chập (Trip Quad) TSQ (Thermo) WS 2005 Waters Micromass Q-Tof Premier Waters Micromass Q-Tof Premier Phun: Z-spray T-Wave = ion optics ... phun điện ESI (Electrospray Ionization) LC -MS • Tách LC Xác định MS • Khó khăn: loại ảnh hưởng dung môi thành phần pha động; thư viện phổ • Để ghép nối LC MS: • giao diện ion hoá hoá học áp suất...

Ngày tải lên: 24/05/2014, 20:42

... high-performance liquid chromatography coupled with diode array detector and mass spectrometry (HPLCDAD-ESI -MS) and to assess the quality of two commercial mycelial AC products Methods Plant Powdered mycelium ... natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI -MS/ MS) Anal Chim Acta 2006, ... Leung: Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD -MS) Chinese Medicine 2010...

Ngày tải lên: 13/08/2014, 15:21

... Interferences 5.1 General In certain cases, isobaric and non-isobaric interferences may occur The most important interferences in this respect are coinciding masses and physical interferences from ... FeC, SOO 68Zn Ba++, Ce+ + FeN, PCl, ArS, FeC, SS, ArNN, SOO Se Zn In the presence of elements in high mass concentrations, interferences may be caused by the formation of polyatoms or doubly charged ... accordance with ISO 5667-1, ISO 5667-2, and ISO 5667-3 Due to the extremely high requirements concerning purity in trace and ultratrace analysis any impurity shall be avoided The mass concentrations...

Ngày tải lên: 03/07/2016, 18:45

... metal surfaces that promote decomposition 6.10.2 The GC /MS interface should allow the capillary column or transfer line exit to be placed within a few mm of the ion source Other interfaces, for ... (DECAFLUOROTRIPHENYLPHOSPHINE, DFTPP) Mass (M/z) Relative Abundance Criteria Purpose of Checkpoint1 51 10-80% of the base peak Low -mass sensitivity 68

Ngày tải lên: 22/03/2014, 14:20

... 6:118 processes and possibly to find new therapeutical targets or diagnostic markers for the disease Combining 2-dimensional gel electrophoresis (2-DE) with mass spectrometry (MS) have recently ... West Pico solution (Pierce Biotechnology, Rockford, USA) The chemiluminescence was detected using MAN-X X-ray system (Fujifilm Science Imaging systems, USA) Developed films for quantitative analysis ... Vivaspin 500 concentrators (Vivascience, Hannover, Germany) supernatants were concentrated and desalted The protein concentration was determined using BCA Protein Assay Kit (Pierce Biotechnology,...

Ngày tải lên: 12/08/2014, 18:22

... following modifications: for every MS scan four CID-induced MS/ MS scans were acquired; MS mass tolerance was set to +/10 ppm for precursors; and +/- 0.6 Da for MS/ MS fragment ions An in-house script ... Differential in-gel electrophoresis; ESI: Electrospray ionization; nLC: Nano-flow liquid chromatography; LTQ: Linear ion trap mass spectrometer; MS: Mass spectrometry; MS/ MS: Tandem mass spectrometry; ... Healthcare/Amersham Bioscience) Excised spots were washed twice with 100 μL of 50 mM ammnonium bicarbonate, once with 100 μL of 75% acetonitrile and once with 100 μL of 100% acetonitrile and left to...

Ngày tải lên: 13/08/2014, 13:20

... first order mass spectrometry, the source collision-induced dissociation (source CID) and CID were set at and respectively to obtain the first order precursor ions The quasi -MS/ MS and MS/ MS spectra ... which are referred to as quasi -MS/ MS /MS ions The comparison of the spectra of quasi -MS/ MS and of quasi -MS/ MS /MS may yield the quasi-third order precursor ions The quasi -MS4 spectra may be obtained ... spectrometry; LC-ESI /MS: liquid chromatography-electrospray mass spectrometry; HPLC-DAD: highperformance liquid chromatography-diode array detection; MS/ MS: tandem mass spectrometry; RDA: retroDiels-Alder;...

Ngày tải lên: 13/08/2014, 15:21

... Monitoring) MRM (Multiple Reaction Monitoring) Đối với MS tứ cực chập ba, máy đo khối phổ hai lần liên tiếp (MS/ MS), kỹ thuật ghi phổ MS/ MS có độ nhạy cao thường sử dụng SRM MRM + SRM: cô lập ... với thiết bị phân tích khối Giao diện kết nối GC vào MS tương đối đơn giản Sơ đồ khối cấu tạo thiết bị GC /MS- MS (kiểu tứ cực chập ba) − Với LC /MS, đầu LC dòng dung môi pha động đưa trực tiếp toàn ... cho MS; dễ dàng bảo dưỡng vận hành; thời gian lưu píc sắc kí đồng giảm thiểu tối đa doãng píc Với thiết bị LC /MS, phận ion hoá thiết kế phù hợp với giao diện kết nối LC với MS thường đặt phía MS...

Ngày tải lên: 11/04/2016, 16:30

... mL/min Mass spectrometry was carried out using a 5973 Mass Selective Detector (Hewlett Packard, Wilmington, DE, USA) in electron-ionization mode with an ionization voltage of 70 eV and ion source ... triplicate Recovery percentages of EDTA from Milli-Q and tap water samples are shown in Table In the case of Milli-Q water samples, excellent recovery percentages at each concentration were obtained, ... (concentration: < 0.1 μg/L), EDTA was detected at comparatively high concentration in other river water samples The median concentration of EDTA in river water samples was 115 μg/L and the concentrations...

Ngày tải lên: 05/09/2013, 10:15

... of benzyl acetate, which was produced by cluster formation due to the relatively high concentration of benzyl acetate in the resultant mixture This assignment is supported by its MS/ MS spectrum, ... (m/z 91 by losing one acetic acid) is clearly seen in the MS/ MS spectrum of m/z 151, confirming that the ion at m/z 151 represents protonated benzyl acetate The benzyl acetate signal increased ... monitoring, tandem mass spectrometry (MSn) helps to identify unknown species, validate proposed intermediates and further understand the reaction mechanisms To demonstrate this, an acetylation reaction...

Ngày tải lên: 14/02/2014, 08:20

... mascot distiller v2.1.1.0 (Matrix Science) Search parameters for peptide and MS ⁄ MS mass tolerance were ± 0.1 Da and ± 0.1 Da, respectively, with allowance for two missed cleavages made from ... gel-assisted digestion and mass spectrometry to detect differences in the protein expression profiles of membrane fractions from tumoral and adjacent normal mucosa from colorectal cancer patients Differentially ... prior to MS analysis In our method, we decided to analyze the sample directly by RPLC -MS ⁄ MS three times to obtain a confident protein identification result Using the iTRAQ labeling mass spectrometry, ...

Ngày tải lên: 16/02/2014, 15:20

... in a QSTAR o-MALDI mass spectrometer (Applied Biosystems Inc., Bellarica, CA, USA) MS ⁄ MS analysis was conducted with the QSTAR o-MALDI mass spectrometer The TOF region acceleration voltage ... structural polymorphism of ApoC1 that has been found Circumstantial evidence such as mass difference from the common protein form and an enhanced level of N-terminal truncation suggested that all ... C1 Table MS ⁄ MS analysis of relevant ApoC1 peptides identified by BIOANALYST software To conserve space, ions are rounded to decimal place The m ⁄ z values were accurate to three places ND, not...

Ngày tải lên: 19/02/2014, 05:20

... expression was induced by the addition of isopropyl thio-bD-galactoside (IPTG) to a final concentration of 1.0 mM After h, the cells were collected by centrifugation, and the cell pellets were ... responsible for optimizing the process to produce high yields of fully active ONC This experimental design may also be extended to other protein systems where the undesired presence of additional residues ... MALDI-TOF MS, the two reactions involved in the activation process of (Met1)-ONC (M23L) and established a rapid procedure to activate the enzyme, without the need for reducing it once purified...

Ngày tải lên: 19/02/2014, 12:20

... underivatized oligosaccharides by negative-ion electrospray mass spectrometry (ES -MS) with collision-induced dissociation (CID) and MS/ MS scanning with low picomole sensitivity [13,14] Several ... 220 °C at °CÆmin)1 and to 310 °C at 10 °CÆmin)1 ES -MS Negative-ion ES -MS and CID MS/ MS were carried out on a Micromass (Manchester, UK) Q-Tof mass spectrometer Nitrogen was used as desolvation ... 0.8 1.8 3.3 Glucitol 4-mono-O-acetyl -4Glcol 1.2 1.2 1.4 0.7 Galactitol 1,5-di-O-acetyl 1,2,5-tri-O-acetyl 1,3,5-tri-O-acetyl 1,2,3,5-tetra-O-acetyl 1,3,5,6-tetra-O-acetyl Gal1-2Gal1-3Gal1-2,3Gal1-3,6Gal1-...

Ngày tải lên: 19/02/2014, 12:20

... potentials (E°) of the electron acceptor more negative than )200 mV generally promote Si-face stereospecificity and redox potentials more positive than )200 mV promote Re-face stereospecificity at C4 of ... glucose-6-phosphate dehydrogenase is Si-face specific (E° ¼ )330 mV) and the NAD-dependent malate dehydrogenase is Re-face specific (E° ¼ )170 mV) Most ethanol dehydrogenases are Re-face specific (E° ¼ 200 mV), ... melanogaster is Si-face specific For recent literature on the subject see Berk et al [11] The following eight F420-dependent enzymes have been analysed and shown to be Si-face specific: F420reducing...

Ngày tải lên: 20/02/2014, 03:20

... 51 MS/ MS ÔHumanÕ (67051 entries) 45 MS/ MS ÔHumanÕ & ÔmyosinÕ (144 entries) MS/ MS: BAP31 human 10 MS/ MS: no identification 30 MS/ MS b- or c-actin 36 MS/ MS c-actin MS/ MS: c-actin variants MS/ MS: ... myosin and actin by LC -MS/ MS Analysis of MS/ MS spectra (n) Database entries (n) Identification 28-kDa band 15 MS/ MS Human (67051 entries) 42-kDa band 42 MS/ MS 39 MS/ MS Human (67051 entries) ÔHumanÕ ... c-actin variants MS/ MS: no identification 35 MS/ MS: myosin heavy chain non-muscle B 16 MS/ MS: no identification 39 MS/ MS: myosin heavy chain non-muscle B MS/ MS: myosin variants 346 A Ducret et al (Eur...

Ngày tải lên: 20/02/2014, 23:20

... Rmsd bond lengths (A) Rmsd bond angles () Number of all atoms Number of protein atoms Number of waters Number of inhibitor atoms Baverage (A2) Protein main chain atoms Protein side chain atoms ... ion cyclotron resonance mass spectrometry: a primer Mass Spectrom Rev 17, 135 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron resonance for proteomics and ... using soft ionization mass spectrometry Int J Mass Spectrom 216, 127 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry Mass Spectrom Rev 23,...

Ngày tải lên: 07/03/2014, 17:20

... carbon atoms of OAA/Asp become the first three carbon atoms of aKG/Glu (see Fig 7), while the carbon atoms of acetyl-CoA (AcCoA) become the last two carbon atoms of aKG/Glu The carbon atoms of AcCoA ... have been produced, but either accumulated intracellularly or excreted extracellularly at concentrations lower than the limits of the detection methods To account for these uncertainties, the ... data reconciliation in linear balance systems in general and [31,58] for data reconciliation analysis in metabolite balance systems) indicated that the extracellular net excretion rates of ammonia...

Ngày tải lên: 08/03/2014, 08:20

... until MS analysis High mass MALDI-TOF MS High -mass MALDI-TOF mass spectra of the intact protein complexes were obtained using a MALDI-TOF mass spectrometer (Voyager DE STR; Applied Biosystems, ... resonance mass spectrometry for protein interaction analysis: application to a calmodulin ⁄ target peptide complex Anal Chem 77, 495–503 19 Tang XT, Munske GR, Siems WF & Bruce JE (2005) Mass spectrometry ... measuring complex and high mass samples Proceedings of the 55th ASMS Conference on Mass Spectrometry, Indianapolis, MP082 40 Zimmer JS, Monroe ME, Qian WJ & Smith RD (2006) Advances in proteomics data...

Ngày tải lên: 15/03/2014, 00:20