calcium signalling in plant cells under environmental stress

... Arsenite contamination is rampant in Bangladesh, India, Japan, the USA and many other areas in the world Drinking arsenite-contaminated water has been associated with increased incidence of skin, lung, ... pathway in removing oxidative stress- induced DNA lesions is still unclear, we sought to understand the role of NER in oxidative stress- induced damage protection and telomere-mediated chromosome integrity ... Low GKM, Ting APL, Gopalakrishnan K, Sethu S, Manikandan J, Hande MP Role of DNA repair factors in telomere integrity and genome maintenance in mammalian cells under oxidative stress International...

Ngày tải lên: 14/09/2015, 08:45

... sugar metabolizing enzymes in both sugar and ROS signalling pathways and their Sugar signalling and antioxidant networks in plants co-operation with antioxidant networks in plant cells are presented ... metabolized Invertases (INVs) can in uence plant growth and development either directly (e.g by in uencing source–sink relationships or by acting as a true regulatory protein) or indirectly via sugar signalling ... signalling and biosynthesis gene expression [41] and HXK-dependent signalling interacts positively and negatively with auxin and cytokinin signalling, respectively [19] Glucose and ethylene signalling...

Ngày tải lên: 15/03/2014, 11:20

... knowledge concerning the regulatory complex in animal cells, there is very little comparable information about plant cells It remains a challenge to understand how metabolism can be regulated in a complex ... plants, and preliminary promoter analyses have been conducted only for the E3 genes from A thaliana [19] Thus, the increase in our understanding of regulation of PDC activity in plant cells over the ... expression in flowers might instead reflect mRNAs present in the pollen Mitochondrial PDC Product inhibition As with their mammalian and microbial counterparts, plant PDCs employ a multisite ping-pong kinetic...

Ngày tải lên: 17/03/2014, 09:20

... and was instrumental in gaining new insight into mitochondrial Ca2+ handling, i.e the topic of this review [13] In the chimeric cDNA, an aequorin moiety including an HA1 tag was fused in frame ... Mitochondrial calcium signalling in cell death cytes inhibition of mitochondrial Ca2+ uptake almost doubled the rate of propagation of the calcium wave across the cell [47] In contrast, in BHK cells inhibition ... Mitochondrial calcium signalling in cell death dynamically control it in living cells, is a field of study that literally boomed in the past decade Indeed, although the idea that mitochondria can form an interconnected...

Ngày tải lên: 30/03/2014, 20:20

... 23 μM in U251MG cells and 24 μM in U343MG cells under normoxic conditions after an incubation time of 24 h (Figure 1) In addition, longer incubation with BA led to increased cytotoxicity in both ... cytotoxicity in human malignant glioma cells in a dose-dependent manner (Figure 1) In addition, the cytotoxic effects of BA were increased in both cell lines under hypoxic conditions (Figure 2) In accordance ... line B16F10, BA improved vincristine-induced cytotoxic effects in vitro, in addition to reducing the number of metastases in vivo [7] Sublines of the colon cancer cell line SNUC5 that are resistant...

Ngày tải lên: 09/08/2014, 09:21

... certain 21 receptors move into lipid microdomains in the outer leaflet of the plasma membrane lipid bilayer These domains are coupled to microdomains in the inner leaflet that contain signaling kinases, ... rafts/caveolae in PACAP-induced neuritogenesis and the pertinent signaling pathways in PC12 cells The current study shows that PACAP induces neurite outgrowth in PC12 cells by induction of translocation ... hairpin-loop conformation in the lipid bilayer There are three major domains in the structure of caveolins The scaffolding domain (SCF) (19-21 amino acids in length) is essential for both caveilin...

Ngày tải lên: 12/09/2015, 08:16

... involving the nanoparticle transport routes and mechanisms in human cells A549 cells are a well characterised in vitro model and have been extensively used for assessing cytotoxicity, including ... clinical medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin8, Ireland 2Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Naughton Institute, Trinity ... Scientific Inc., USA) Briefly, cells seeded in 96-wells plates as described above were incubated with the above mentioned SiO2NP, for different intervals as previously indicated in the text, and in addition...

Ngày tải lên: 11/08/2014, 00:23

... stored in an ice box or in a refrigerator maintained at 4°C We started the experiment within 24 h of sampling Tetracycline hydrochloride (Tokyo Chemical Industry, Japan) was dissolved in predetermined ... culture method using the R2A agar medium (Eiken Chemical, Japan) containing 100 mg/L tetracycline The inoculated plates were incubated at 20°C for d After incubation, the colonies obtained on the ... colonies were inoculated in sterilized water samples containing 10 mg/L tetracycline and incubated with shaking at 20°C for d The samples whose absorbance at 356 nm decreased after incubation were...

Ngày tải lên: 05/09/2013, 10:15

... DOM in the original river water sample seems to fit into two linear portions indicating two classes of binding sites In contrast, DOMs in the samples after and weeks fitted to a single line, ... of zinc binding sites in river water DOM Fig represents the variation of DOC, UV254 and SUVA during the river water incubation for weeks in the preliminary study During the first days of incubation, ... slopes of the lines in a particular binding site class Conditional stability constant for the strong type of binding site remained at log K1' = 7.2 while that in weak type remained at log K2'...

Ngày tải lên: 05/09/2013, 10:15

... phosphorylated in response to convulxin (data not shown), suggesting that it may associate with GPVI in the absence of the FcR c-chain Convulxin also induced an increase in calcium in GPVI-expressing Jurkat ... GPVIspecific ligand convulxin, resulting in phosphorylation of the protein kinase Syk, therefore reproducing the proximal events of GPVI signalling in platelets The FcR c-chain is We next performed ... c-chain was observed in cells expressing both wild-type GPVI and FcR c-chain (K562/F1-c), but not in cells expressing only the FcR c-chain (K562/pRc-c) (Fig 6A) This demonstrates that convulxin...

Ngày tải lên: 22/02/2014, 07:20

... receptors can interact directly with structural proteins such as b-actin, a-actinin, laminin a3 and integrin b2 in HEK cells, some of which might initiate cytoskeletal rearrangements following receptor ... mechanistic insight into the P2X7-triggered signalling pathways regulating neurite formation in N2a cells P2X7 receptor inhibition leads to increased neurite formation in N2a cells, this effect occurring ... ATP from interneurons in the dorsal horn region [21], trigeminal nucleus pars caudalis neurons [22] and spinal cord substantia gelatinosa neurons [23] Interestingly, purinergic signalling is already...

Ngày tải lên: 07/03/2014, 02:20

... EhADH2, indicating the presence of two catalytically independent domains, the N-terminal domain, displaying ALDH activity, and the C-terminal domain, containing an iron-binding domain, which is involved ... to inactivation under aerobic conditions Our results indicated that, under saturating O2 conditions, the enzyme was fully inactivated after a short incubation (30 min) At this time, EhPFOR inactivation ... E Pineda et al Fermenting enzymes and oxidative stress in Entamoeba samples was also applied where indicated The number of independent preparations assayed is indicated in parentheses Modeling...

Ngày tải lên: 15/03/2014, 23:20

... b-CN(1–25)4P] This result, obtained in an experimental model consisting of in vitro cells, is in agreement with results obtained using animals or everted intestinal tissue [21–24] It is FEBS ... only maintain Ca2+ in an absorbable form but also interact with the plasma membranes of certain cells, facilitating Ca2+ uptake 5006 Concerning the conflicting results of human studies, some in favor ... Intestinal absorption of calcium in rats given diets containing casein or aminoacid mixture: the role of casein phosphopeptides Br J Nutr 49, 67–76 38 Sato R, Noguchi T & Naito H (1986) Casein...

Ngày tải lên: 16/03/2014, 05:20

... during winter and anoxia during summer, in combination with the changes in salinity, are associated with high mortality in this species [25–27] In view of CHH involvement in response to stress in ... of PO staining with PO-CHH antiserum (A, B) Intrinsic multipolar cells, located in anterior (A) and posterior (B) bars (C, D) Cells at · 1200 magnification (E) PO-CHH staining shown in nerve fibers ...  3-fold increase in glucose and a more than 30-fold increase in lactate, whereas the levels in controls remained constant The arginine kinase gene (AK) and the eukaryotic translation initiation...

Ngày tải lên: 16/03/2014, 06:20

... examined the effects of SA on stress response in mammalian cells using a simple screening system, and revealed that SA is a potent Hsp inducer in mammalian cells, thereby protecting cells against ... HeLa cells were grown in Eagle’s Minimum Essential medium (Nissui Pharmaceutical) containing 10% bovine serum in a CO2 incubator at 37 °C Screening for Hsp modulators A reporter plasmid containing ... into C3H10T1/2 cells (1 · 107 cells per 100 mm dish) with 30 lL lipofectAMINE reagent (Invitrogen) according to the manufacturer’s instructions, and incubated for 48 h Cells were then maintained...

Ngày tải lên: 17/03/2014, 10:20

... matrix MPLI-induced apoptosis of brain cells The final question is whether MPLI-induced elevation of brain dopamine levels results in changes in the brain cells To examine this, brains dissected ... flows into the brain from the hemolymph This was confirmed by the in ux of [3H]dopamine into the brain after its injection into the hemocoel of MPLI-injected larvae (Fig 2) Radiolabeled dopamine incorporated ... the injection Dopamine incorporation into MPLI-treated larval brains The delay in reaching maximal brain dopamine level compared with maximal hemolymph dopamine level suggested that dopamine...

Ngày tải lên: 23/03/2014, 21:20

... coverslips and maintained in DMEM, containing 2% horse serum, for 24 h before prosaposin treatment (10 nm for at 37 °C) and labelling Cells were rinsed, and fixed in 4% formaldehyde in NaCl ⁄ Pi for ... +prosaposin Prosaposin/GM1 70 Cells with yellow staining (%) LRP-1 Control C Gα0 Control Control A Prosaposin signalling through lipid rafts Fig Scanning confocal microscopic analysis of prosaposin, ... Prosaposin signalling through lipid rafts M Sorice et al or filipin III almost completely abolished the prosaposin-induced SphK1 activation, indicating that the prosaposin-triggered signalling pathway...

Ngày tải lên: 24/03/2014, 00:21

... PIN genes in dicotyledonous Arabidopsis have been studied in detail, including AtPIN1–AtPIN4 and AtPIN7, which act in auxin efflux transport, but the function of AtPIN5, AtPIN and AtPIN8 remains ... protein containing a Mem_trans domain (PF03547.11) was chosen, and after removing the PIN-like proteins, the remaining proteins were considered SbPIN proteins AUX and LAX1–3 protein sequences in ... linker domain connecting the first nucleotide-binding domain and the second transmembrane domain is seen in all SbPGPs A second nucleotide-binding domain in the C-terminus of SbPGP10 proteins is absent...

Ngày tải lên: 29/03/2014, 09:20

... containing CCh, which induced a second calcium increase and a further increase in Ins(1,4,5)P3 (Fig 3Aa) Both of these calcium increases were accompanied by increased Ins(1,4,5)P3, indicating ... contain calcium- binding domains [6], and other Ins(1,4,5)P3-metabolizing enzymes, including inositol polyphosphate 3-kinases and inositol polyphosphate 5-phosphatases, are regulated by calcium ... intracellular calcium concentration, the increase in Ins(1,4,5)P3 was sustained In addition, the subsequent increase in calcium was less effective at enhancing the increase initial receptor-activated Ins(1,4,5)P3...

Ngày tải lên: 30/03/2014, 03:20

... during CRC carcinogenesis, an effective cytotoxic effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells ... cyclin A2 in 5-FU-treated KIAA0247-overexpressing cells was 69% of that in these cells without 5-FU treatment However, this cyclin A2 downregulation was not detected in the shLuc cells Cyclin ... determined the expression of the uncharacterized gene, KIAA0247, in human gastrointestinal tissues and colonic cell lines Results indicated that KIAA0247 ubiquitously expresses in gastrointestinal...

Ngày tải lên: 18/06/2014, 19:20