division of labour in plant cells
Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... confirmed by sequencing DNA samples of 51 healthy individuals. Interestingly, this SNP had not yet been registered in any SNP databases despite of its high frequency of 20% in samples of patients ... of samples containing the C/T SNP downstream of exon 10 led to the identification of cleavage products of approximately 101 bp and 444 bp in size in samples either containing the heterozygous ... (SNP) in the intron sequence flanking the 3’ end of exon 10 in 7 out of 35 (20%) patient samples. The SNP consisted of a C > T exchange (Figure 1 C-E) and was positioned at nt 67 of the intron...
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The noneffects of class on the gendered division of labor in the home
... mode of production level of analysis, involving comparisons of capitalist class structures with different kinds of noncapitalist classstructures, or at a more concrete level of analysis, involving ... gender is the problem of bargaining power between men and women within households. Parti-cularly in an era in which gender roles are being challenged, the division of labor in the household should ... andgender. Instead of asking how the gender division of labor withinfamilies varies across locations within a class structure, the focus of analysis would be on how the gender division of labor...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... was obtained with scFv4A-d1-EGFP and 7A-d1-EGFP expressed in parallel.Interestingly, in the case of 4A (but not 7A), we founda minor percentage of cells with a faint cytoplasmicstaining (arrow). ... FRAP:measuring protein mobility and activity in living cells. Nature Cell Biol 3, 145–147.34 Bachmair A, Finley D & Varshavsky AK (1986) In vivohalf-life of a protein is a function of its amino-terminalresidue. ... killing following binding(recently reviewed in [1]). Whilst a variety of cell typesincluding plant cells, fungal cells and even bacteria [2]have been described as hosts, mammalian cells are themost...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... POUhomeodomain family proteins bind to this consensus motifand regulate gene transcription in diverse cells throughinteraction with a variety of partner DNA binding proteins,including Sp1 family members ... thepivotal role of fgl2 in a murine model for murine hepatitisvirus strain 3 (MHV-3) induced fulminant hepatic failure[21,22]. Administration of neutralizing antibodies againstfgl2 conferred ... development of a normal fetus and in regulatingimmune responses within the intestine. Induction of fgl2 in macrophages in response to viruses results in theproduction of a potent coagulant activity...
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Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx
... withuntreated cells. As expected, cells exposed to KN93also developed more neurites than control cells, point-ing to a key role of this kinase in the control of neuritogenesis in N2a cells (Fig. 5E).Involvement ... currents in this cell line. Pharmacological inhibition of the P2X7 receptor and its knockdown by small hairpin RNA interferenceresulted in increased neuritogenesis in cells cultured in low serum-contain-ing ... adhesion kinase is upstream of phosphati-dylinositol 3-kinase ⁄ Akt in regulating fibroblast survival in response to contraction of type I collagen matricesvia a beta 1 integrin viability signaling...
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Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx
... andnucleolin are involved in the endocytosis of hLf into CHOK1 cells. Fig. 7. Binding of human lactoferrin (hLf) to nucleolin expressed onMDA-MB-231 cells. The figure shows the green fluorescence intensitybound ... binding to surface nucleolin suggests that they shouldshare a common binding site in nucleolin, i.e. the C-terminaltail containing nine RGG repeats. The conservation of thisnucleolin domain ... theseFig. 10. Prediction of nucleolin-binding sites in human lactoferrin (hLf).Sequences of the N2 and C2 domains of hLf, bovine lactoferrin (bLf)and human transferrin (hTf) were aligned and...
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Báo cáo khoa học: Sugar signalling and antioxidant network connections in plant cells pptx
... is capable of reducing oxidant-induced mod-ifications of proteins during exposure of yeast cells toH2O2[101]. The ability of trehalose to reduce intracel-lular oxidation during dehydration ... remain unclear [43].Plants possess three types of invertase: acid cell wallinvertases (glycoside hydrolase family 32) located in the apoplast (i.e. the continuum of cell walls of adja-cent plant ... ethylenesignalling converge at the level of EIN3 protein stabil-ity [42].Role of sucrose splitting enzymesSucrose is one of the most widespread disaccharides in nature. In higher plants it represents...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... 1A,B).The inhibition of insulin signalling in the liver is pri-marily reflected by the attenuation of the insulin-medi-ated inhibition of gluconeogenic gene expression. AsEffect of TNFa on insulin ... critical in maintaining cir-culating glucose levels and, in a preliminary investigation using the humanhepatoma (HepG2) cell line in this study, we demonstrated the role of TNFa in the regulation of ... immunofluoresence stainingwith anti-Foxa2 IgG. In cells incubated with 50 nminsulin, Foxa2 was fairly strongly detected in the cyto-sol, when compared with cells incubated in the absence of insulin. Although...
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Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf
... sequestering of intracel-lular iron m ay protect from oxidative damage induced by awide range of stresses [38]. Indeed, an increase of ferritinmRNA has been observed in A. thaliana leaves photo-inhibited ... ferritins are mainly cytosolic proteins, the plant ones appear to be localized in chloroplasts of plant cells or, more i n general, in plastids [8,15]. In this work it isshown t hat ferritins ... drops of blocking buffer: 1% (w/v) bovine serumalbumin, 20% (v/v) normal goat serum in 0.1MTris-buffered saline (pH 7.4), and then incubated for 2 h in Tris-buffered saline (pH 7.4) containing...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... many proteins involved in apoptosis, including the expression profiles of proteins involved in the mito-chondrial (bcl-2, bax, and bcl-x, etc.) and stress reac-tion-related pathways of apoptosis ... transfected cells and control cells. Interestingly, the phosphorylation of JNK/SAPK was inhibited by the Int. J. Med. Sci. 2007, 4 40Figure 2 PowerBlot patterns of JAR cells. Forty elements of ... overexpressed in cytotrophoblasts, and a decrease in the expression of Bcl-2 and increase of apoptotic cells are seen in preeclampsia. Thus antiapoptotic proteins can play an important role in preventing...
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