biochemical functions in metabolism and protein modification
Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... USA) and transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein ... CM (1998) Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA Nucleic Acids Res 26, 5388–5393 17 Stauffer ME & Chazin WJ (2004) Physical interaction ... origin-containing DNA in vitro with purified proteins Proc Natl Acad Sci USA 84, 1834– 1838 Wold MS & Kelly T (1988) Purification and characterization of replication protein A, a cellular protein...
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Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes potx
... structures, chemical modifications and cross-linking in protein mixtures, complexes, and assemblies Proteins A single protein, a list of proteins from a mixture or from a protein complex or a complete ... and artificially introduced cross-links in proteins Cross-links impose distance constraints on amino acid residues that can be used to model the 3-D structure of proteins and protein complexes [3–7] ... the amino terminus and at lysine residues, and because modification at lysine residues prevents cleavage by trypsin Accordingly, analysis using virtualmslab including modifications with NEM in the...
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Báo cáo khoa học: "Comparative analysis of heart functions in micropigs and conventional pigs using echocardiography and radiography" docx
... aeroK ,ytisrevinU lanoitaN luoeS ,ettimmoC esU dna eraC laminA lanoitutitsnI eht fo serudecorP noitarepO dradnatS eht htiw ecnadrocca ni tuo deirrac erew stnemirepxe lamina llA slaminA sdohteM ... htiw deniatniam dna ,detalitnev yllacinahcem ,detabutni ,liatkcoc enimatelit/mapezaloz a dna ,enizalyx ,eniporta htiw detacidemerp dna thginrevo detsaf erew slamina ehT noitcnuf caidrac fo tnemssessa ... ,tar( slamina yrotarobal llams ro namuh eht ot derapmoc sA noitalitnev lacinahcem dna aisehtsena lareneg rednu erew yeht elihw sgiporcim eht no demrofrep saw yhpargoidracohcE stluseR tnacifingis...
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Báo cáo y học: "GC- and AT-rich chromatin domains differ in conformation and histone modification status and are differentially modulated by Rpd3" doc
... pairs of interacting chromatin segments (via protein/ protein/DNA cross-links) Second, cross-linked chromatin is solubilized and then digested and ligated at low concentration so that cross-linked ... efficiency due to cross-linking of proteins to restriction sites and that any differences in cross-linking in GC- and ATrich domains should be detectable as differences in their susceptibilities ... datalevelcross-linking efficiency in GC- and Additionalforbetweenquantify andcross-linking and efficiencies of GC- here data file withand seven histone modifications in relation Relationship file in is...
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Báo cáo sinh học: " The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment" docx
... envelopment and virusinduced cell fusion [30] In this manuscript, we show that the amino and carboxyl termini of UL20p contain distinct domains that function in infectious virion production and intracellular ... encoding glycoprotein B (gB) [13,14], and the UL53 gene coding for glycoprotein K (gK) [15-19] Of these four membrane associated proteins, only UL20 and gK are absolutely essential for the intracellular ... Hoflack B: A tyrosine-based motif and a casein kinase II phosphorylation site regulate the intracellular trafficking of the varicella-zoster virus glycoprotein I, a protein localized in the trans-Golgi...
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Báo cáo hóa học: " Correlating novel variable and conserved motifs in the Hemagglutinin protein with significant biological functions" docx
... The HA protein is on the surface of the influenza particle and is involved in receptor attachment and binding and antigenic determinants This study reports unique regions in the protein that ... MEME block and their conservation in the years studied, in addition to its overlap with a single receptor binding site infers block importance in selective and specific receptor binding and host ... Binding Site A (Green), Antigenic Binding Site B (Magenta), Antigenic Binding Site C (Red), Antigenic Binding Site D (Red) tional modification, enhance pathogenicity by mutating receptor binding...
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Báo cáo y học: "Activator protein 1 (Fos/Jun) functions in inflammatory bone and skin disease" pps
... genes encoding the Ca2+-binding proteins S100a8 and S100a9 in keratinocytes upon deletion of JunB and Jun, both in vivo and in vitro The S100a8 and S100a9 genes map to the PSORS4 region and have ... Bennett B, Karin M, Yang L, Manning AM, Firestein GS: c-Jun N-terminal kinase is required for metalloproteinase expression and joint destruction in inflammatory arthritis J Clin Invest 2001, 108:73-81 ... activator protein transcription factor The dimeric activator protein (AP-1) transcription factor is composed of Jun and Fos proteins Jun proteins form homodimers or heterodimers with Fos proteins through...
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Báo cáo y học: "Circulating retinol binding protein 4 in critically ill patients before specific treatment: prognostic impact and correlation with organ function, metabolism and inflammation" pot
... of insulin resistance into the circulation, thereby providing a link between adipocytokines, inflammation and systemic insulin resistance [7,8] Retinol binding protein (RBP4), a 21-kDa protein ... PM: Retinol-binding protein- 4 in experimental and clinical metabolic disease Expert Rev Mol Diagn 2008, 8:289-299 10 Wolf G: Serum retinol-binding protein: a link between obesity, insulin resistance, ... TK, Vanden Berghe G: Adiponectin, retinol-binding protein 4, and leptin in protracted critical illness of pulmonary origin Crit Care 2009, 13:R112 16 Gressner OA, Koch A, Sanson E, Trautwein C,...
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Tài liệu Use Variables and Functions in T-SQL pptx
... Initialing Local Variables in T-SQL To initialize the variables, you will use the SET command, shown in these two lines of code: SET @Cust_Id = 'ANTON' SET @Order_Date = '11/27/1996' By setting ... the initial values, you are then ready to use the variables within the rest of your procedure, any way that you need them, again, by using the @varname syntax Utilizing Built -In Functions Within ... a Windows Form Then place the controls listed in Table 6.2 and seen in Figure 6.3 with the following properties set Table 6.2 Control Property Settings for This How-To Object Property Setting...
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Tài liệu Báo cáo khoa học: Domain deletions and substitutions in the modular protein evolution doc
... domains, for example ‘set6’ contained proteins with six domains Each protein from a given set containing proteins of length N domains was compared with each protein from the set containing proteins ... domains that were found to form single-domain proteins; b fraction of deleted domains that were found to form singledomain proteins; c average number of neighbours for all domains in the protein ... domain deletions at the termini regions are partly related to domain versatility and their ability to function outside a multidomain protein (to form single-domain proteins) The events involving...
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Tài liệu Báo cáo khoa học: Abstract Integration of Metabolism and Survival PP-1 The metabolic switch in liver methionine metabolism pptx
... significant increase in integrin alpha6 and decrease in integrin alpha v and beta4 expression levels In the presence of fibronectin and/ or HGF, a significant increase in integrin alpha3 and alpha5 expression ... activates the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and WASP interacting protein (WIP) in neuroblastoma cell line, SH-SY-5y ... examining a protein – DNA interaction In this method the protein- dependent association of two fluorescent-labelled DNA fragments each containing about half of a sequence defining a protein- binding...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... H, Martinez R, Canoll P, Peles E, Musacchio JM, Plowman GD, Rudy B & Schlessinger J (1995) Protein tyrosine kinase Pyk2 involved in Ca2+induced regulation of ion channel and MAP kinase functions ... and Gq-mediated pathways on calcium ⁄ calmodulin, Pyk2, and Src kinase J Biol Chem 272, 19125–19132 Luttrell LM (2002) Activation and targeting of mitogenactivated protein kinases by G -protein- coupled ... lacking the Ca2+ ⁄ CaM-binding IQ domain, did not increase the basal or serotonininduced ERK1 ⁄ phosphorylation The ERK1 ⁄ phosphorylation was inhibited in the presence of the calcium in ux inhibitor,...
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Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc
... (PMA), insulin and ATP on the distribution of protein kinase C (PKC) isoforms Hepatocytes cultured for h and 48 h were incubated with 0.1 lM PMA, 10 nM insulin or 0.1 lM ATP for min, and subsequently ... glycogen synthase, in an insulindependent manner [49], supports the hypothesis of crosstalk between insulin and integrin-signalling pathways The lack of an insulin-elicited increase in DAG, shown here ... dexamethasone is known to retain cuboidal hepatocyte morphology in cultures and to in uence actin polymerization [48], the differences in insulin signalling (and also in EGF signalling) observed between...
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Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
... insulin and amino acids in isolated rat hepatocytes Eur J Biochem 269, 3742–3750 Meijer, A.J & Sauerwein, H.P (1999) Amino acid-dependent signal transduction and insulin sensitivity Curr Opin Clin ... limiting Therefore, the effect of different activators of AMPK and the effect of inhibitors of protein phosphatases on the amino-acid-induced activation of ACC and p70S6K were examined in freshly ... 129/111; glutamine and leucine, 530/ 500 mUÆg cells)1, n ¼ 2) Inhibitors of protein phosphatases exert different effects on ACC and p70S6K activities The effect of two inhibitors of protein phosphatases,...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... glycine, l-alanine, l-proline, l-lysine, l-serine, b-alanine, taurine, sarcosine, dimethylglycine, glycine betaine, trimethylamine N-oxide, and cytidine 2¢-3¢ cyclic monophosphate were also obtained ... misfolded proteins [5–8] and remove protein aggregation [9–12] Mechanisms of protein osmolyte interactions, the effect of osmolytes on protein stability, and how osmolytes correct protein misfolding ... effects that urea has on protein function [16–19] Despite significant advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship...
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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
... GAMT in EAC and S180 cells and in sarcoma tissue indicates that the upregulated proteins are entirely tumor-cell specific On the other hand, the in ux and efflux rate of creatine in both EAC and ... arginase I and ODC direct ornithine towards polyamine synthesis and the mitochondrial enzymes arginase II and ornithine aminotransferase favor the channeling of ornithine to proline ⁄ glutamine ... estimation of proline and ornithine J Biol Chem 199, 91–95 45 Van Pilsum JF, Martin RP, Kito E & Hess J (1956) Determination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methyl-guanidine...
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Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... suggesting that the DNA polymerase activity of this enzyme may be important for plant cells On the other hand, the N-terminal BRCT domain proposed to mediate protein protein interactions involved in ... 000 g for 20 min, and the soluble protein fraction was collected as the crude extract and loaded on to 10 mL His-Bind Resin (Novagen) The column was washed with 50 mL binding buffer and then with ... 552 amino acid residues with a molecular mass of 60.9 kDa OsPol k protein contains a BRCT domain at the N-terminus and a Pol X domain at the C-terminal region, similar to the overall domain organization...
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Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
... containing CLP and CLAP with a phosphoprotein stain did not reveal phosphate additions to these proteins Only lanes in the gel containing the known phosphoproteins ovalbumin, b-casein and pepsin ... domains or putative GPI unit, if one exists in CLAP as it does in Drosophila fasciclin I and many other fasciclin-containing proteins [36,46] Fas I domains in proteins are almost always found in ... fasciclin domains in CLAP with other fasciclin I containing proteins is clear (Fig 9) Using BLAST (http://www.ncbi.nlm.nih.gov/BLAST) to identify related proteins, a putative cell adhesion protein...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D ... disrupting protein protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon...
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