binding of metal complexes on the surface of macromolecular carriers
Handbook of Polymer Synthesis Second Edition Episode 11 pptx
... change of the electrostatic potential, etc Kind and strength of the formed bonds between metal and ligand depend on the ionisation potential of the metal ion, its electron affinity and the donor properties ... Characteristic of both investigated systems is the strong compression of hydrogel volume with increasing amount of the metal ion Attention must be paid to the fact of the influence of the degree of macroligand ... macroligand ionization on the character of the conformational change of the linear segments of the gel It is seen that under high pH the swelling coefficient of the PEI gel passes through a maximum in the...
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Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
... solutions were prepared The first was the gel-filtration elution buffer containing the 43-kDa protein incubated for 30 at room temperature; and the second was the gel-filtration elution buffer containing ... Representative surface plasmon resonance sensorgrams of binding between the 43-kDa protein and the 4-kDa peptide were dependent on concentration Details of the procedure are described in the Materials ... the function of these molecules is to bind to their target proteins to regulate or inhibit their activities, it is assumed that the function of the 4-kDa peptide also relates to the regulation...
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Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc
... FACSCalibur (Becton-Dickinson, San Jose, CA, USA) Surface biotinylation of proteins To analyze the stability of CD38 on the plasma membrane of the different Ba/F3 mutants, labeling of the surface proteins ... homodimers (data not shown) These results suggest that the stabilization of CD38 homodimers by crosslinkers depends strongly on the conformation and orientation between the CD38 monomers It has been ... extended expression on the plasma membrane Enzyme activity is not dependent on the presence of stable homodimers As the mutations in the cytoplasmic region and the a1-helix of CD38 affected dimer...
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báo cáo hóa học:" Unsaturated phosphatidylcholines lining on the surface of cartilage and its possible physiological roles" potx
... contributions YC contributes to the conception and design, the conduction of the experiment, the collection, analysis and interpretation of data, the drafting the manuscript and acquisition of ... also synthesized and secreted in other parts of the body such as in the peritoneal cavity and joints [4,5], where its adsorption on the surfaces of tissues at these sites has been demonstrated ... in the lung regions of the body The present results offer further support to the Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and Research 2007, 2:14 opinion...
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Báo cáo toán học: " Spin-related tunneling through a nanostructured electric-magnetic barrier on the surface of a topological insulator" potx
... polarized along the direction of the vector (π × z), the magnetic field bends the trajectory of the electrons, resulting in a rotation of the spin of the transmitted electrons The reflected electrons suffer ... trajectories of the electrons and the outgoing electrons will propagate in the same direction as the incident ones The spin of the reflected electron, with reversed longitudinal wavevector and conserved ... barrier on the surface of a three-dimensional topological insulator We found that (1) the propagating behavior of electrons in such system exhibits a strong dependence on the direction of the incident...
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Báo cáo lâm nghiệp: "Interactions of ozone and pathogens on the surface structure of Norway spruce needle" potx
... infection in ambient air fumigation The fumigation with higher ozone concentrations could be sterilizing The effect of the ozone fumigation and fungal infection on the epicuticular wax structure seemed ... study The ozone concentration used in the experiment carried out by Schmitt et al (1987) was too low (100 ,ug) to cause significant surface erosion in the material analyzed in our study Ozone concentrations ... the needles is interesting at least as an intensifying factor in the erosion caused by other air pollutants In the current study, the surface in the needles infection in ambient air fumigation...
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Báo cáo y học: "Identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture" doc
... impact on improving the laboratory diagnosis of prosthetic hip joint infections Conclusion A wide range of bacteria can be found on the surface of prosthetic hip joints removed at revision arthroplasty ... methods One of the major problems in accurately determining the infection rate is the difficulty in isolating, by traditional culture methods, the bacteria from the surface of the prosthetic hip ... culture of the same samples The clinical interest of this study is the presence of any organism on the prosthetic hip joints and the role, if any, that they have in initiating, pro- Page of 11...
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Báo cáo y học: " HIV-1 neutralization by monoclonal antibody against conserved region 2 and patterns of epitope exposure on the surface of native viruses" pot
... of this epitope on the surface of this HIV-1 subtype B However, this study is preliminary and further experiments will be required to confirm these observations Conclusion The differences amongst ... http://www.jibtherapies.com/content/7/1/5 Peptides corresponding to conserved neutralizable epitopes on C1 and C2 regions of gp120 (HIV-1 CRF01_AE) The designation of peptides corresponding C1 (C1E1 ... nm The cutoff is defined as the mean value of absorption of serum samples from mice immunized with normal saline or that of fresh culture medium Immunization and monoclonal antibody production...
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Báo cáo y học: "HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
... than the level of HLA-DR in the cell lysates (5ul of a in 10 dilution, from x 106 PBMC) The shed HLA-DR therefore seemed to represent a small fraction of the total HLA-DR of the cell, however the ... respective surface expression Our results support the observations seen in the study of Fumeaux et al (2002) demonstrating that a modification of HLADR, post-translationally, may be one mechanism responsible ... expression in regulated in sepsis The demonstration that GM-CSF increased the shedding of HLA-DR from the cell surface and also increased HLA-DR gene transcription may have implications for the therapy...
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Effect of urban emissions on the horizontal distribution of metal concentration in sediments in the vicinity of Asian large cities
... mgZn/kg based on the uniform distribution of these metals in the sediments of the lake The higher concentration in the northwest part of the lake is probably due to reverse flow of the polluted Pasig ... untreated wastewater contained higher concentrations of lead and zinc The data on the metal concentration of wastewater in the Philippines is not available In the case of other countries, Karvelas ... Accuracy of the measurement was confirmed through the analysis of Laguna Lake samples, as shown in the result section of the paper In addition, the accuracy was also confirmed through the monitoring...
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Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
... because these are the most distant pair of haems in the structure and are therefore expected to have the weakest interaction [33] The pH dependence of the chemical shifts of the NMR signals of the ... contribution to the reduction potential of the centres is small [40,41], and also with the experimental observation for monohaem cytochromes that the effect of anion binding or complex formation on the reduction ... undisturbed by the replacement of Fe by Zn in the rubredoxin given the identical structures of the two protein forms [38,39] Therefore, at the concentrations used in the NMR experiments over 90% of the...
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
... to the iron(III) of Fe(ToCPP) and Fe(ToCPP))13G10 The second part of our results concerns the binding of imidazole derivatives on the iron of Fe(ToCPP) either alone in solution or inside the binding ... (Table 1) The binding of the ®rst CN± ligand to the iron was thus more easy in the hydrophobic binding pocket of the antibody than the binding of the second one, most probably because of the steric ... iron of Fe(porphyrin))13G10 complexes: (A) binding of H2O2 on the iron of 13G10-(Fe(ToCPP), (B) binding of two CN± ligands on the iron of 13G10-(Fe(ToCPP), (C) binding of H2O2 and imidazole on...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... demonstrating the utility of the grafting of functional peptides with randomized edge sequences for optimizing the orientation of the grafted peptide on a scaffold protein In addition, when the ... the design of and screening for small binding proteins The preparation of the small binding proteins with binding function further allows us to increase the binding strength by multibinding approaches, ... structural analyses and the construction of artificial libraries allow constructive selection and functionalization not only of antibody fragments, but also of small, nonantibody proteins (Fig...
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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
... than the M3 in MtSTP Crystal contacts at the active site The differential occurrence of the metal ions correlates with the conformation of the flap subdomain (132–174) and the binding of adjacent ... binding of the third metal ion suggesting, like Pullen et al [9], that rearrangement of the flap domain in HsSTP might lead to binding of a third metal ion The implications of the M3 binding and ... adjacent STP monomers over the active sites of monomers C and D, but not of A and B (Fig 3A) For monomer C, which contains three metal ions, the monomer binding over the active site is monomer A...
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Báo cáo khoa học: A kinetic approach to the dependence of dissimilatory metal reduction by Shewanella oneidensis MR-1 on the outer membrane cytochromes c OmcA and OmcB potx
... for the in situ immobilization and containment of contaminant metals and radionuclides in aqueous waste streams and subsurface environments, as some of these metals precipitate upon reduction ... reduction, as characterized by IC50s of 10.7 lm and 81.4 lm for inhibition of OmcA and OmcB, respectively Modes of inhibition of either OmcA or OmcB by V(V) The modes of inhibition of either OmcA- ... no upregulation or downregulation of FR (P ¼ 0.83) On the basis of the fact that FR is not subjected to regulation under the applied conditions, and deducing from Fig 2C that all other c-type...
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Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
... additional contacts with the molecule A 2164 Catalytic site The catalytic reaction of glucoamylases proceeds with inversion of configuration at the anomeric carbon which requires a pair of carboxylic ... addition revealed the presence of a second acarbose (substrate analogue) ˚ binding site on the surface of the enzyme, 25 A remote from the catalytic site The key residues involved in the binding at ... glucoamylases demonstrate that mutations of the amino acids proposed to be involved in binding of the surface acarbose caused reduction of enzyme adsorption on starch, proving that these amino acids...
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Báo cáo Y học: Membrane embedded location of Na+ or H+ binding sites on the rotor ring of F1F0 ATP synthases ppt
... different positions on the fatty acid chain The uorescence yields depend on the spinlabel position and the concentration of the labelled lipids The relation of these results to the depth of the uorophore ... same residue of the enzyme This conclusion was corroborated by the inhibition of PCD labeling in the presence of Na+ which resembles the effect of this coupling ion on the reaction of cE65 with ... accessibility of the binding site from the aqueous environment The modication of the binding sites by DCCD was specically protected by Na+ that conrms the direct access of Na+ to the c subunit...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... respect to the role of HSP70 in the inhibition of the release of Smac and apoptosis GAPDH Ratio of HSP70 to GAPDH 14 # 12 # 10 * The role of the ATP -binding domain of HSP70 in the prevention of the ... cytotoxicity The protective effects of HSP70 were correlated with the level of HSP70 expression because the clone with higher HSP70 expression demonstrated a more significant reduction of the apoptotic ... Fig ATP -binding domain of HSP70 is essential for the inhibition of H2O2-induced activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation...
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